Review
Agriculture, Dairy & Animal Science
Francisco A. Uzal, Federico Giannitti, Javier Asin
Summary: Yellow lamb disease is a poorly understood condition in young sheep caused by certain strains of Clostridium perfringens type A. It is characterized by hemolysis, jaundice, and anemia. Diagnosis of the disease is challenging due to the presence of the microorganism in the intestines of healthy sheep. There is currently no specific vaccine for yellow lamb disease.
Article
Biochemistry & Molecular Biology
Julia Bruggisser, Ioan Iacovache, Samuel C. Musson, Matteo T. Degiacomi, Horst Posthaus, Benoit Zuber
Summary: This study describes the structure of Clostridium perfringens beta-toxin (CPB) and its formation of an octameric pore complex with unique structural features. The research also identifies CPB as a member of a new subclass of the hemolysin-like family. Additionally, the study demonstrates that the structure of the pore can be modified without affecting its pore-forming ability, making it attractive for macromolecule sensing and nanotechnology. The cryo-electron microscopy structure of the CPB octameric pore will contribute to future developments in nanotechnology and basic research.
Article
Food Science & Technology
Masahiro Nagahama, Keiko Kobayashi, Masaya Takehara
Summary: The study found that lysosomal proteases cathepsin B and L facilitate the internalization of iota-toxin by activating ASMase, indicating their role in the cytotoxicity caused by the toxin.
Article
Agriculture, Dairy & Animal Science
Jill Derix, Richard Ducatelle, Bart Pardon, Evi Croes, Niels Groot Nibbelink, Linda Van Deurzen-Duineveld, Filip Van Immerseel, Evy Goossens
Summary: Necro-hemorrhagic enteritis in calves, caused by Clos-tridium perfringens type A, is a fatal disease affecting calves in intensive rearing systems. Lack of development of active immunity against alpha toxin is the main trigger. This study investigates the effect of milk replacer components on alpha toxin production and the effect of lactose on antibody production.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Microbiology
Keita Yanagimoto, Eiji Haramoto
Summary: This study elucidated the distribution of PLC-negative C. perfringens type F in sewage and proposed independent detection of this strain. The presence in sewage may reflect community hygiene, highlighting the importance of the study.
MICROBIOLOGY SPECTRUM
(2021)
Article
Pathology
Llorenc Grau-Roma, Mauricio Navarro, Sohvi Blatter, Christian Wenker, Sonja Kittl, Francisco A. Uzal, Horst Posthaus
Summary: Several outbreaks of necrotic enteritis-like disease in lorikeets were consistently isolated Clostridium perfringens. The alpha toxin gene of C. perfringens was detected in a high percentage of affected lorikeets, while the beta toxin gene was also found in a significant number of cases. Other toxin genes were detected inconsistently and in fewer samples.
VETERINARY PATHOLOGY
(2021)
Article
Veterinary Sciences
Mohammad Ali Shamshirgaran, Mehdi Golchin, Mahmoud Salehi, Reza Kheirandish
Summary: This study aimed to evaluate the recombinant Lactobacillus casei expressing the C-terminal domain of alpha-toxin from Clostridium perfringens as a potential probiotic-based vaccine candidate for immunizing broiler chickens against necrotic enteritis (NE). The results showed that orally immunized broiler chickens with the recombinant vaccine strain were significantly protected against experimental NE challenge and developed specific serum anti-alpha antibodies. Additionally, the immunized birds exhibited higher body weight gains compared to control groups during the challenge experiment.
BMC VETERINARY RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
John W. Finnie, Francisco A. Uzal
Summary: Clostridium perfringens type D epsilon toxin (ETX) causes severe neurological disease in ruminant livestock and is of worldwide distribution. The toxin can reach the brain through the bloodstream, leading to neurologic syndromes. ETX is also listed as a potential bioterrorism agent.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Luis F. Soto, Ana C. Romani, Gabriel Jimenez-Avalos, Yshoner Silva, Carla M. Ordinola-Ramirez, Rainer M. Lopez Lapa, David Requena
Summary: This study computationally screened the whole proteome of Clostridium perfringens and identified highly immunogenic proteins, domains, and epitopes. They found potential recombinant vaccine candidates, a potential sub-unit vaccine, and designed a multi-epitope protein. These findings are important for the development of safe and effective vaccines against C. perfringens infection.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Jie Li, Xiaoyu Huang, Kaihui Xie, Juanli Zhang, Jiaojiao Yang, Zunqiang Yan, Shuangbao Gun
Summary: S100A9 plays an important role in C. perfringens type C-induced infectious diarrhea. Overexpression of S100A9 exacerbates cell damage and inflammatory response, inhibits cell proliferation, and disrupts cell-cell tight junctions. In contrast, inhibition of S100A9 expression alleviates inflammatory injury caused by infectious diarrhea.
Article
Immunology
Lore Van Damme, Natasja Cox, Chana Callens, Michelle Dargatz, Monika Fluegel, Sarah Hark, Frank Thiemann, Stefan Pelzer, Freddy Haesebrouck, Richard Ducatelle, Filip Van Immerseel, Evy Goossens
Summary: Clostridium perfringens produces ECM-degrading enzymes that play a role in avian necrotic enteritis. Different variants of the colA gene encoding collagenase were identified, with some variants linked to necrotic enteritis strains. Truncated collagenases may contribute to the pathology of necrotic enteritis by degrading collagen type IV in the intestinal villi.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Food Science & Technology
Wei Wan, Yue Li, Jing Wang, Zhiying Jin, Wenwen Xin, Lin Kang, Junhong Wang, Xiaoyang Li, Yakun Cao, Hao Yang, Jinglin Wang, Shan Gao
Summary: In this study, a new type of dissolving microneedle patch (dMN) with a nanoparticle adjuvant was developed to enhance immune response and deliver the rETX(Y196E)-C protein vaccine. The dMN vaccine showed good mechanical properties, immunogenicity, and protection, effectively preventing ETX poisoning.
Article
Biotechnology & Applied Microbiology
Mohammad Ali Shamshirgaran, Mehdi Golchin, Elham Mohammadi
Summary: In this study, a recombinant Lactobacillus casei strain expressing the NetB protein of Clostridium perfringens was used as a probiotic-based vector vaccine to immunize broiler chickens against necrotic enteritis. The vaccinated birds showed significant protection against the disease and developed strong antibody responses. This study suggests that a probiotic-based vector vaccine could be a promising candidate for controlling necrotic enteritis in broiler chickens.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Xiu Zeng, Baosheng Liu, Jiao Zhou, Yimin Dai, Chunsheng Han, Linkang Wang, Yunzheng Wu, Jinhua Zhang
Summary: Clostridium perfringens is a common opportunistic pathogen with pathogenicity dependent on toxin production. Analysis of the genome of a piglet-derived strain revealed the presence of two toxin genes, with cpb2 located on a large plasmid.
SCIENTIFIC REPORTS
(2021)
Article
Food Science & Technology
Siyu Zhang, Lingling Ma, Fuyang Song, Dong Wang, Kesong Shi, Yong Li, Jin Zeng, Yujiong Wang
Summary: This study found that Clostridium perfringens toxin CPB-1 induces macrophage and endothelial cell pyroptosis through the NLRP3 inflammasome and Caspase-1 pathway. The results suggest that NLRP3 may be a potential therapeutic target for Clostridium perfringens disease.
Article
Multidisciplinary Sciences
Wael Abu Dayyih, Raghad Layth, Mohammad Hailat, Bayan Alkhawaja, Lina Al Tamimi, Zainab Zakaraya, Aseel Aburumman, Nisreen Al Dmour, Mohamed J. Saadh, Hisham Al-Matubsi, Saed M. Aldalaen
Summary: In this study, it was found that administering date molasses to rats significantly decreased the pharmacokinetic parameters of levetiracetam, which could increase the risk of epileptic seizures.
SCIENTIFIC REPORTS
(2023)
Article
Materials Science, Multidisciplinary
Anjan Kumar, Ali K. Al-Mousoi, Mohamed J. Saadh, Mustafa K. A. Mohammed, G. V. S. S. Sarma, Nafis Ahmad, Ratnesh Tiwari
Summary: The use of tin oxide as an electron transport layer has improved the efficiency and stability of perovskite solar cells.
ELECTRONIC MATERIALS LETTERS
(2023)
Review
Environmental Sciences
Mohamed J. Saadh, Hala Baher, Yuanji Li, Mvnl Chaitanya, Jose Luis Arias-Gonzales, Omer Qutaiba B. Allela, Mohammed H. Mahdi, Juan Carlos Cotrina-Aliaga, Natrayan Lakshmaiya, Salam Ahjel, Ali H. Amin, Gregorio Gilmer Rosales Rojas, Fuad Ameen, Muhammad Ahsan, Reza Akhavan-Sigari
Summary: Multidisciplinary approaches in cancer treatment are essential in coordinating multiple disciplines for effective tumor elimination. Nanocarriers have emerged as promising structures for the treatment of pancreatic cancer by responding to pH and redox conditions, allowing for targeted delivery of therapeutic cargo. Loading drugs onto nanoparticles helps enhance intracellular accumulation in cancer cells, slowing the progression of pancreatic cancer. Nanoparticles can also be used for gene encapsulation, protecting genes from degradation and promoting intracellular accumulation for tumor suppression. In addition, nanotechnology enables selective targeting of pancreatic cancer cells, reducing side effects on normal cells.
ENVIRONMENTAL RESEARCH
(2023)
Review
Immunology
Mohamed J. Saadh, Khadijehsadat Kazemi, Hossein Khorramdelazad, Mohammad Javad Mousavi, Negar Noroozi, Maryam Masoumi, Jafar Karami
Summary: Evidence suggests that T cells play a role in the development of SLE and have different metabolic pathways. Metabolic processes and enzymes determine the function of T cells in inflammatory and autoimmune responses. Dysregulation of metabolism in SLE T cells has been found, and drugs targeting autoreactive T cell metabolism could be a promising therapeutic approach for SLE treatment.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Nutrition & Dietetics
Mohamed J. Saadh, Rashmi Saxena Pal, Jose Luis Arias-Gonzales, Juan Carlos Orosco Gavilan, J. C. Darshan, Mohamed Mohany, Salim S. Al-Rejaie, Abolfazl Bahrami, Mustafa Jawad Kadham, Ali H. Amin, Hrosti Georgia
Summary: This study used Mendelian randomization (MR) analysis to investigate the causal relationship between variable risk factors and various nutrients with inflammatory bowel diseases (IBDs). The results showed that certain genetically predicted risk factors and nutrients are associated with the occurrence of IBDs. These findings provide new evidence and suggestions for the treatment and prevention of IBDs.
Article
Agriculture, Dairy & Animal Science
Mohamed Jamal Saadh
Summary: This study aimed to determine whether red grape seed extract (RGSE) alleviated the changes in brain-derived neurotrophic factor (BDNF) level, acetylcholinesterase activity, oxidative stress, and apoptosis induced by orally administered malathion in a rat model of malathion-induced neurotoxicity. The results showed that RGSE treatment improved the changes in these biochemical parameters, thus substantially controlling oxidative stress and apoptosis in the brains of rats.
Article
Computer Science, Information Systems
Khaled Matrouk, V Srikanth, Sumit Kumar, Mohit Kumar Bhadla, Mirza Sabirov, Mohamed J. Saadh
Summary: This study explores the method of dynamic social network alignment and finds that it can reveal more apparent patterns for aligning "Social Web of Things" networks. Through experiments on real-world datasets, it demonstrates that this method outperforms the current mainstream algorithms in terms of alignment effectiveness.
ACM JOURNAL OF DATA AND INFORMATION QUALITY
(2023)
Article
Pharmacology & Pharmacy
Mohamed Saadh
Summary: The experimental drug Ruthenium Complex II has shown promise as a highly cytotoxic treatment for cancer, with minimal harm to healthy cells. It causes apoptosis in cancer cells and holds great potential as a targeted therapy.
Article
Pharmacology & Pharmacy
Mohamed Saadh
Summary: This study synthesized and studied the potential of ruthenium complex II as a therapeutic agent in cancer treatment. It was found to have cytotoxic activity against MCF-7 cancer cells with an IC50 value of 7.56 μg/mL and cytotoxic activity against MRC-5 normal cells with an IC50 value of 576.6 μg/mL. The ruthenium complex II showed selective cytotoxicity against MCF-7 cancer cells and demonstrated apoptotic activity through the activation of caspase-3.
Article
Chemistry, Multidisciplinary
Inci Sogutlu, Shakir Mahmood Saeed, Mohaned Adil, Anupam Yadav, Evan Abdulkareem Mahmood, Mohamed J. Saadh
Summary: This study developed a novel aqueous two-phase system (ATPS) using deep eutectic solvents (DES) combined with polyethylene glycol dimethyl ether 250 and two types of NADESs. The experimental results showed that the hydrogen bonds of NADESs in the mixed solutions may not be completely disrupted, forming ternary-like systems. Additionally, the binodal data fitted well using the Merchuk and Zafarani-Moattar equations. Furthermore, the ATPSs demonstrated good extraction levels for amino acids such as l-arginine, l-phenylalanine, and l-tyrosine. These findings provide a basis for the development of improved extraction methodologies and exploring new applications in biotechnology and pharmaceuticals.
Article
Materials Science, Multidisciplinary
N. T. Talat, A. A. Dahadha, M. Abunuwar, A. A. Hussien, Wafa'a Odeh
Summary: The study investigated the influence of polyethylene glycol 400, 4000, and polyvinylpyrrolidone K15 as cost-effective, efficient, and eco-friendly inhibitors on the corrosion inhibition of copper in H2SO4 solution through weight-loss, thermometric, and electrical conductance techniques. The addition of PEG 400, PEG 4000, and PVP K15 to the corrosive solutions significantly inhibited the corrosion rates of copper at various temperatures. PVP K15 was found to be a more efficient inhibitor than PEG 400 and PEG 4000, possibly due to the nature of the functional groups and the size of the PVP K15 chains. The adsorption of PEG 400, PEG 4000, and PVP K15 on copper surface followed the Langmuir and Temkin adsorption isotherm models, and thermodynamic aspects of the adsorption process were calculated and discussed.
INTERNATIONAL JOURNAL OF CORROSION AND SCALE INHIBITION
(2023)
Article
Pharmacology & Pharmacy
Lina N. Tamimi, Zainab Zakaraya, Wael Abu Dayyih, Thana Y. ALjaraedah, Rima H. Al Omari, Mohammad Abu Assab, Mohamed J. Saadh, Basim Mareekh, Mohammed F. Hamad
Summary: Current anti-diabetic drugs have many side effects, so alternative strategies with minimal side effects and low cost are needed. This study evaluated the role of selenium yeast (SY) in diabetes mellitus (DM) induced by Streptozotocin (STZ). The results showed that SY can reduce blood glucose and lipid levels, alleviate inflammation, and restore antioxidant capacity, suggesting its potential therapeutic role in diabetes.
JOURNAL OF POPULATION THERAPEUTICS AND CLINICAL PHARMACOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Anjan Kumar, M. I. Sayyed, Michael M. Sabugaa, Mohammed Al-Bahrani, Shilpa Sharma, Mohamed J. Saadh
Summary: Density Functional Theory (DFT) was used to study the interaction between ClCN and CNC. The research suggests that pristine CNC is not suitable for detecting ClCN gas. Various methods, including functionalization and metal decoration, were applied to enhance the properties of CNC. Simulation results show that doping with aluminium and gallium atoms yields promising results.
Review
Medicine, Research & Experimental
Mohamed J. Saadh, Maria V. Mikhailova, Soheil Rasoolzadegan, Mojgan Falaki, Roozbeh Akhavanfar, Jose Luis Arias Gonzales, Amir Rigi, Bahman Abedi Kiasari
Summary: Introduces the emerging therapeutic modality of mesenchymal stem/stromal cells (MSCs) therapy for the treatment of inflammatory bowel disease (IBD). MSCs alleviate dysregulated inflammatory responses through the secretion of anti-inflammatory mediators and their effects on gut immuno-microbiota regulation and the proliferation and differentiation of intestinal cells. Clinical trials are being conducted to evaluate the safety and efficacy of MSCs administration in IBD patients.
EUROPEAN JOURNAL OF MEDICAL RESEARCH
(2023)
Article
Pharmacology & Pharmacy
L. N. Tamimi, Z. Zakaraya, M. Hailat, W. Abu Dayyih, E. Daoud, A. Abed, M. J. Saadh, B. Majeed, H. Abumansour, A. Aburumman, J. M. Majeed, M. Hamad
Summary: This study aimed to explore the combined use of resveratrol and pioglitazone against streptozotocin-induced diabetes mellitus. The combination of these two drugs significantly decreased fasting blood glucose, insulin, HbA1c, and insulin resistance levels, as well as alleviated lipid disturbances and inflammation. It also restored total antioxidant capacity and peroxisome proliferator-activated receptor levels.
EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES
(2023)