Journal
JOURNAL OF EXPERIMENTAL BOTANY
Volume 67, Issue 11, Pages 3313-3323Publisher
OXFORD UNIV PRESS
DOI: 10.1093/jxb/erw147
Keywords
Ammonium; Arabidopsis; thaliana; broccoli; glucosinolates; myrosinase; nitrate; nitrogen nutrition; proteomics
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Funding
- Basque Government [IT932-16]
- Spanish Ministry of Economy and Competitiveness - FEDER [AGL2015-64582-C3-1-R, BIO2014-56271-R]
- People Programme (Marie Curie Actions) of the European Union under REA grant [334019]
- ISCIII [PT13/0001]
- FEDER
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A quantitative proteomic approach demonstrates how ammonium nutrition induces glucosinolate biosynthetic and catabolic pathways in Arabidopsis and broccoli.Accessing different nitrogen (N) sources involves a profound adaptation of plant metabolism. In this study, a quantitative proteomic approach was used to further understand how the model plant Arabidopsis thaliana adjusts to different N sources when grown exclusively under nitrate or ammonium nutrition. Proteome data evidenced that glucosinolate metabolism was differentially regulated by the N source and that both TGG1 and TGG2 myrosinases were more abundant under ammonium nutrition, which is generally considered to be a stressful situation. Moreover, Arabidopsis plants displayed glucosinolate accumulation and induced myrosinase activity under ammonium nutrition. Interestingly, these results were also confirmed in the economically important crop broccoli (Brassica oleracea var. italica). Moreover, these metabolic changes were correlated in Arabidopsis with the differential expression of genes from the aliphatic glucosinolate metabolic pathway. This study underlines the importance of nitrogen nutrition and the potential of using ammonium as the N source in order to stimulate glucosinolate metabolism, which may have important applications not only in terms of reducing pesticide use, but also for increasing plants' nutritional value.
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