Article
Cell Biology
Venera Khuzakhmetova, Ellya Bukharaeva
Summary: Studies on the effects of catecholamines on synaptic transmission at neuromuscular junctions have not answered the main question about their mechanism on quantal release. Interest in catecholamines has increased due to their potential use in treating various diseases, but their effects on acetylcholine release and the underlying mechanisms remain unclear. Research on noradrenaline and adrenaline showed differences in their effects on acetylcholine secretion, indicating a specificity that depends on the type of muscle being targeted in clinical practice.
CELLULAR AND MOLECULAR NEUROBIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Ellya Bukharaeva, Venera Khuzakhmetova, Svetlana Dmitrieva, Andrei Tsentsevitsky
Summary: Adrenoceptor activators and blockers are commonly used in the clinical treatment of cardiovascular, pulmonary, and neurodegenerative diseases by regulating neurotransmission at the neuromuscular junction. Understanding the molecular mechanisms of these drugs may lead to the development of highly effective and well-tolerated sympathomimetics for treating various neurodegenerative diseases with synaptic defects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Kenji Takikawa, Hiroshi Nishimune
Summary: Synaptic transmission is crucial for motor control and brain functions. Presynaptic molecules play a vital role in regulating neurotransmitter release. Dysfunction of presynaptic molecules can lead to various neurological disorders and cognitive decline in aging.
Article
Cell Biology
Achmed Mrestani, Martin Pauli, Philip Kollmannsberger, Felix Repp, Robert J. Kittel, Jens Eilers, Soren Doose, Markus Sauer, Anna-Leena Siren, Manfred Heckmann, Mila M. Paul
Summary: During PHP, individual AZs undergo compaction without changes in the copy numbers of AZ proteins. This compaction even occurs at the level of Brp subclusters and RBP subclusters, indicating a reorganization of AZ components. Correlative confocal and dSTORM imaging shows apparent increases in AZ area and Brp protein content during PHP.
Article
Biochemistry & Molecular Biology
Nikita Zhilyakov, Arsenii Arkhipov, Artem Malomouzh, Dmitry Samigullin
Summary: Cholinergic neurotransmission plays a key role in both the peripheral and central nervous systems, but certain aspects of its regulation remain unclear. Studies suggest that nicotine can decrease ACh release by enhancing calcium influx through Ca(v)1 channels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Neurosciences
Xinying Huang, Junjian Jiang, Jianguang Xu
Summary: Understanding the degeneration and regeneration mechanisms of neuromuscular junctions after denervation and reinnervation is clinically valuable and may lead to the development of therapies for maintaining or protecting the structures and functions of NMJs.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2022)
Article
Neurosciences
Kosala N. Dissanayake, Filip Margetiny, Charlotte L. Whitmore, Robert C-C Chou, Cornelia Roesl, Vishwendra Patel, Joseph J. McArdle, Richard Webster, David Beeson, John E. H. Tattersall, David J. A. Wyllie, Michael Eddleston, Richard R. Ribchester
Summary: Intentional ingestion of agricultural organophosphorus insecticides is a significant public health issue in rural Asia, leading to thousands of deaths annually. Cyclohexanol alters synaptic transmission at neuromuscular junctions, reducing postsynaptic sensitivity to acetylcholine while enhancing evoked transmitter release.
JOURNAL OF PHYSIOLOGY-LONDON
(2021)
Article
Developmental Biology
Jun Yu, Marilyn Janice Oentaryo, Chi Wai Lee
Summary: The study demonstrates the essential role of local protein synthesis in presynaptic differentiation at developing neuromuscular junctions. Newly synthesized proteins play a crucial role at agrin bead-neurite contacts. Axonal MT1-MMP translation is shown to be vital in mediating agrin-induced presynaptic differentiation, in addition to the recognized long-range transport of MT1-MMP proteins.
Article
Neurosciences
Matheus P. S. Magalhaes-Gomes, Wallace Camargos, Priscila A. C. Valadao, Rubens S. Garcias, Hermann A. Rodrigues, Jessica N. Andrade, Vanessa P. Teixeira, Ligia A. Naves, Walter L. G. Cavalcante, Marcia Gallaci, Silvia Guatimosim, Vania F. Prado, Marco A. M. Prado, Cristina Guatimosim
Summary: Increased VAChT expression leads to higher levels of ACh release, but may have detrimental effects on the structure and function of the neuromuscular system, including impaired synaptic vesicle cycle and structural alterations of NMJs.
Article
Neurosciences
Peter H. Chipman, Richard D. Fetter, Lauren C. Panzera, Samuel J. Bergerson, Daniel Karmelic, Sae Yokoyama, Michael B. Hoppa, Graeme W. Davis
Summary: Homeostatic plasticity is a physiological process that counteracts neuronal perturbations in the brain. Acute AMPAR-specific antagonism can induce compensatory plasticity, leading to potentiation of presynaptic neurotransmitter release. This process can be induced within minutes and requires postsynaptic NMDARs.
Article
Neurosciences
Ryan J. Durbin, Dante J. Heredia, Thomas W. Gould, Robert B. Renden
Summary: Neurotransmission is influenced by extracellular pH. Alkalization enhances transmitter release and receptor activation, while acidification inhibits these processes and can activate acid-sensitive conductances in the synaptic cleft. The direction of change differs across synapse types. At the mammalian neuromuscular junction (NMJ), the direction and magnitude of pH transients in the synaptic cleft during transmission remain unclear. In this study, researchers investigated the extracellular pH transients at the cholinergic synapse of the mouse NMJ under near physiological conditions and identified their sources. They found that activity-induced intracellular Ca2+ release in the muscle plays a role in cleft alkalization, whereas PMCA inhibition decreases alkalization. This suggests that cholinergic synapses at the mouse NMJ typically alkalize due to cytosolic Ca2+ liberation during activity, unless under highly strenuous conditions where acidification predominates.
JOURNAL OF NEUROSCIENCE
(2023)
Article
Neurosciences
Qiao Ding, Kaamini Kesavan, Kah Meng Lee, Elyse Wimberger, Thomas Robertson, Melinder Gill, Dominique Power, Jeryn Chang, Atefeh T. Fard, Jessica C. Mar, Robert D. Henderson, Susan Heggie, Pamela A. McCombe, Rosalind L. Jeffree, Michael J. Colditz, Massimo A. Hilliard, Dominic C. H. Ng, Frederik J. Steyn, William D. Phillips, Ernst J. Wolvetang, Shyuan T. Ngo, Peter G. Noakes
Summary: A defect in the n-agrin-LRP4-MuSK signaling pathway has been identified in muscle from MND patients for the first time. This signaling pathway plays a crucial role in maintaining the stability of the neuromuscular junction. These findings provide a potential therapeutic target for prolonging muscle function in MND patients.
ACTA NEUROPATHOLOGICA COMMUNICATIONS
(2022)
Article
Critical Care Medicine
Ana Paula Silveira Leite, Carina Guidi Pinto, Felipe Cantore Tiburcio, Kevin Silva Muller, Carlos Roberto Padovani, Benedito Barraviera, Rui Seabra Ferreira Junior, Claudenete Vieira Leal, Cintia Yuri Matsumura, Selma Maria Michelin Matheus
Summary: Peripheral nerve injuries lead to changes in the Agrin-LRP4-MuSK pathway, resulting in disaggregation of AChRs and a shift from epsilon to gamma subunit. This study evaluated the effect of combining tubulization with fibrin biopolymer on AChRs and associated proteins after peripheral nerve injury. The results showed that the tubulization with fibrin biopolymer group exhibited morphological and morphometric similarities to the control group at the neuromuscular junctions. Additionally, there was an increase in S100 and AChR epsilon protein expression, and a decrease in MyoD expression. These positive associations enhanced AChRs stabilization and promoted neuromuscular regeneration, highlighting the potential of tubulization for severe injury repair and the beneficial effect of fibrin biopolymer.
INJURY-INTERNATIONAL JOURNAL OF THE CARE OF THE INJURED
(2023)
Article
Behavioral Sciences
Marc Bosque, Ramon Margalef, Albert Llaveria, Manel M. Santafe
Summary: This study aims to evaluate the relationship between stress and spontaneous neurotransmission. It was found that both acute and chronic stress significantly increased spontaneous neurotransmission. This may be related to the genesis or maintenance of myofascial pain.
BEHAVIOURAL BRAIN RESEARCH
(2023)
Article
Medicine, Research & Experimental
Alexey M. Petrov, Guzalia F. Zakirjanova, Irina Kovyazina, Andrei N. Tsentsevitsky, Ellya A. Bukharaeva
Summary: This study investigated the influence of nerve activity and adrenoceptor stimulation on exocytosis modes in the motor nerve terminal. It was found that high frequency activity promoted neurotransmitter release via kiss-and-run mechanism, while noradrenaline and alpha 2-adrenoreceptors agonist favored full-collapse exocytosis. This has implications for the regulation of respiratory system during stress.
Article
Chemistry, Physical
Bulat Faizullin, Aidar Gubaidullin, Tatiana Gerasimova, Ilya Kashnik, Konstantin Brylev, Kirill Kholin, Irek Nizameev, Alexandra Voloshina, Guzel Sibgatullina, Dmitry Samigullin, Konstantin Petrov, Elvira Musina, Andrey Karasik, Asiya Mustafina
Summary: This study introduces a novel heterometallic Agx-Re6 nanocrystalline colloid, which exhibits different surface charges and binding properties in various solution environments. The research found that PEI treatment can affect the surface properties and cytotoxic mechanisms of the colloids.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2022)
Article
Chemistry, Multidisciplinary
Bulat Faizullin, Irina Dayanova, Igor Strelnik, Kirill Kholin, Irek Nizameev, Aidar Gubaidullin, Alexandra Voloshina, Tatiana Gerasimova, Ilya Kashnik, Konstantin Brylev, Guzel Sibgatullina, Dmitry Samigullin, Konstantin Petrov, Elvira Musina, Andrey Karasik, Asiya Mustafina
Summary: This study introduces a simple electrostatically driven approach to engineer highly cytotoxic nanomaterials. These nanomaterials can aggregate and disassemble in lysosomal environments under pH-triggered conditions. The localization extent of these nanomaterials in lysosomes can be determined through their luminescence response, and their cytotoxicity mechanism can be revealed by detecting the rupture of lysosomal membranes.
Article
Biochemistry & Molecular Biology
M. A. Suslov, G. Sibgatullina, D. Samigullin
Summary: This paper describes the design and implementation of a CO2 automation system for a laboratory cell incubator that allows the construction of a controlled CO2 incubator using available laboratory equipment. The article presents test results on cell cultures to demonstrate the functionality of the design. Since CO2 incubators are important and widely used scientific equipment, the development information provided in this article will be useful for a wide range of researchers.
JOURNAL OF EVOLUTIONARY BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Chemistry, Physical
Svetlana Fedorenko, Dinara Farvaeva, Alexey Stepanov, Olga Bochkova, Kirill Kholin, Irek Nizameev, Sergey Drobyshev, Tatiana Gerasimova, Alexandra Voloshina, Elizabetta Fanizza, Nicoletta Depalo, Guzel Sibgatullina, Dmitry Samigullin, Konstantin Petrov, Aidar Gubaidullin, Asiya Mustafina
Summary: This study introduces the factors controlling the encapsulation of oleate-oleylamine-stabilized Cu((2-x))S nanoparticles into silica nanocomposites through the water-in-oil microemulsion method. The study also explores their fluorescence, aggregation behavior, and therapeutic function. The results show that recharging the Cu((2-x))S nanoparticles aids in their encapsulation, while interfacial post-treating of the nanocomposites affects aggregation behavior and ROS generation. The treated nanocomposites exhibit higher cytotoxicity under low power NIR-irradiation, attributed to the heating-induced increase in ROS generation.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
(2022)
Article
Chemistry, Medicinal
Iliza Ramazanova, Maxim Suslov, Guzel Sibgatullina, Konstantin Petrov, Svetlana Fedorenko, Asiya Mustafina, Dmitry Samigullin
Summary: Magnetic nanoparticles (MNPs) are actively used in various biomedical applications. This study focuses on the influence of magnetic field on MNPs internalized into living cells and the manipulation of MNPs using electromagnetic needles. The study also evaluates the attractive force and viscosity of HeLa cancer cells. The findings provide insights into the potential applications of MNPs in biomedicine.
Article
Biochemistry & Molecular Biology
Marat A. Mukhamedyarov, Aydar N. Khabibrakhmanov, Venera F. Khuzakhmetova, Arthur R. Giniatullin, Guzalia F. Zakirjanova, Nikita V. Zhilyakov, Kamilla A. Mukhutdinova, Dmitry V. Samigullin, Pavel N. Grigoryev, Andrey V. Zakharov, Andrey L. Zefirov, Alexey M. Petrov
Summary: Amyotrophic lateral sclerosis (ALS) is a disease characterized by skeletal muscle denervation, motor neuron loss, and respiratory failure. Mutations in the FUS gene are a common genetic cause of ALS, resulting in degeneration. In mutant FUS mice at the pre-onset stage, early structural and functional alterations in diaphragm neuromuscular junctions (NMJs) were observed. Lipid peroxidation and decreased lipid raft staining were found in the mutant mice. Despite preserved end-plate structure, increased levels of presynaptic proteins and impaired neurotransmitter release were observed in FUS mice. These findings suggest that alterations in membrane properties, synapsin 1 levels, and calcium kinetics could be early indicators of nascent NMJ pathology in ALS.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Leysan Vasileva, Gulnara Gaynanova, Farida Valeeva, Grigory Belyaev, Irina Zueva, Kseniya Bushmeleva, Guzel Sibgatullina, Dmitry Samigullin, Alexandra Vyshtakalyuk, Konstantin Petrov, Lucia Zakharova, Oleg Sinyashin
Summary: Liposomes modified with tetradecyltriphenylphosphonium bromide loaded with ff-tocopherol and donepezil hydrochloride were designed for intranasal administration. The physicochemical characteristics of the liposomes were within the desired range. In vitro release analysis showed that the cationic surfactant-modified nanocontainers could penetrate into rat motoneurons mitochondria. Imaging of rat brain slices confirmed the penetration of nanocarriers into the brain. Experiments on transgenic mice demonstrated that intranasal administration of the liposomes resulted in improved learning abilities and reduced plaque formation in the brain.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Andrei N. Tsentsevitsky, Chulpan R. Gafurova, Kamilla A. Mukhutdinova, Arthur R. Giniatullin, Nikita S. Fedorov, Artem I. Malomouzh, Alexey M. Petrov
Summary: In this study on the diaphragm neuromuscular junctions of mice, it was found that the release of neurotransmitters is regulated by secretory sphingomyelinases (SMases), which enhance mobilization of synaptic vesicles and facilitate full fusion mode of exocytosis while suppressing neurotransmitter transmission when acting on vesicular membranes.
Article
Biochemistry & Molecular Biology
Guzalia F. Zakirjanova, Arthur R. Giniatullin, Chulpan R. Gafurova, Artem I. Malomouzh, Nikita S. Fedorov, Arthur N. Khaziev, Andrei N. Tsentsevitsky, Alexey M. Petrov
Summary: This study investigates the effects of enzymatic cholesterol oxidation on neuromuscular transmission in mice diaphragms. The results show that cholesterol oxidation disrupts lipid-ordering in synaptic membranes, decreases synaptic delay and exocytosis probability, enhances neurotransmitter and dye release, and inhibits extracellular acetylcholine accumulation. Additionally, cholesterol oxidation affects the mode of exocytosis and may be related to lipid raft disruption.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2023)
Article
Biochemistry & Molecular Biology
Julia G. Odnoshivkina, Guzel V. Sibgatullina, Alexey M. Petrov
Summary: This study discovers that neurotransmitter release from sympathetic terminals plays a crucial role in heart regulation. Monitoring presynaptic exocytotic activity in mice atrial tissue, it is found that high [K+]o depolarization causes the release of FFN511, a false fluorescent neurotransmitter, and this release is augmented by the neurotransmitter uptake inhibitor reserpine. In conclusion, neurotransmitter release is essential for heart regulation, and can be influenced by factors such as depolarization and neurotransmitter uptake inhibitors.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2023)
Article
Biochemistry & Molecular Biology
Leysan Vasileva, Gulnara Gaynanova, Darya Kuznetsova, Farida Valeeva, Anna Lyubina, Syumbelya Amerhanova, Alexandra Voloshina, Guzel Sibgatullina, Dmitry Samigullin, Konstantin Petrov, Lucia Zakharova
Summary: This research demonstrates the potential of cationic liposomes to target mitochondria in tumor cells and selectively release mitochondrial toxins. This finding is significant for the development of anticancer therapies.
Article
Biochemistry & Molecular Biology
Andrei N. Tsentsevitsky, Venera F. Khuzakhmetova, Ellya A. Bukharaeva, Alexey M. Petrov
Summary: This study found that alpha 2-adrenoreceptors (ARs) are important regulators of neuromuscular transmission through G protein-gated inwardly rectifying potassium (GIRK) channels, and synaptic vesicle exocytosis depends on calcium influx through voltage-gated calcium channels (VGCCs).
NEUROCHEMICAL RESEARCH
(2023)
Article
Chemistry, Physical
Olga Bochkova, Svetlana Fedorenko, Artem Mikhailov, Gennadiy Kostin, Maxim Mikhailov, Maxim Sokolov, Julia Elistratova, Kirill Kholin, Maxim Tarasov, Yulia Budnikova, Guzel Sibgatullina, Dmitry Samigullin, Irek Nizameev, Vadim Salnikov, Ivan Yakovlev, Darina Rozhentsova, Anna Lyubina
Summary: This study reports the synthesis of silica nanoparticles incorporated with two photoactive components, which exhibit both synergistic and anti-synergistic effects. The amino-group modification on the surface of the nanoparticles facilitates the deposition of cluster units, resulting in heterometallic core-shell nanoparticles. The protein corona formed on the surface of these nanoparticles promotes high colloidal stability and efficient cellular internalization. Cytotoxicity measurements demonstrate the synergistic effect of the nanoparticles in dark conditions and the anti-synergistic effect in photo-induced cytotoxicity.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
(2024)
