Article
Biochemistry & Molecular Biology
Lucas Bayones, Samuel Alfonso-Bueno, Mauricio Montenegro, Jose Moya-Diaz, Octavio Caspe, Luciana Gallo, Fernando D. Marengo
Summary: This study investigated the endocytosis and replenishment of the immediately releasable pool (IRP) in mice primary chromaffin cells. The researchers found that not only a fast dynamin-dependent endocytosis occurred after the release of about 50% IRP, but also a fast dynamin-independent endocytosis was activated after depolarizations lasting 25-50ms, which released over 80% of IRP. This dynamin-independent endocytosis was found to be Ca2+- and protein kinase C (PKC)-dependent. Additionally, PKC inhibition also affected the slow component of replenishment observed after total IRP exocytosis.
JOURNAL OF NEUROCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Mauricio Montenegro, Lucas Bayones, Jose Moya-Diaz, Cecilia Borassi, Andres Martin Toscani, Luciana I. Gallo, Fernando D. Marengo
Summary: The study shows that replenishment of immediately releasable pool (IRP) in chromaffin cells consists of a slow and a rapid component, linked respectively to fast endocytosis and cytosolic Ca2+ concentration. Furthermore, the stability of cortical F-actin affects the performance of both rapid replenishment and fast endocytosis.
JOURNAL OF NEUROCHEMISTRY
(2021)
Article
Biology
Sebastien Houy, Joana S. Martins, Noa Lipstein, Jakob Balslev Sorensen
Summary: This study investigated the relationship between phorbolesters and Munc13-1 and ubMunc13-2, and found that phorbolesters only stimulated secretion when ubMunc13-2 was dominant. The results showed that DAG/phorbolesters, ubMunc13-2, and Syt7 are important factors for stimulating dense-core vesicle priming.
Article
Biochemistry & Molecular Biology
M. T. Alonso, P. Torres-Vidal, B. Calvo, C. Rodriguez, A. Delrio-Lorenzo, J. Rojo-Ruiz, J. Garcia-Sancho, S. Patel
Summary: Evidence has shown that acidic organelles can accumulate and release Ca2+ upon cell activation. Monitoring the dynamics of Ca2+ in these compartments is crucial for understanding their physiopathological aspects. Bioluminescent GECIs have advantages in acidic compartments due to their low pH sensitivity, low background noise, no phototoxicity, no photobleaching, high dynamic range, and tunable affinity. This article reviews the use of bioluminescent aequorin-based GECIs targeted to acidic compartments and highlights the need for more measurements in highly acidic compartments.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2023)
Article
Multidisciplinary Sciences
Pengcheng Zhang, David Rumschitzki, Robert H. Edwards
Summary: This study investigates the release of secretory vesicles in mouse adrenal chromaffin cells and discovers diverse modes of release. Through comprehensive analysis, it is revealed that the variations in release cannot be solely explained by stochasticity, but involve distinct mechanisms. Furthermore, the loss of calcium sensor synaptotagmin 7 increases the proportion of slow events without altering the intrinsic properties of either class, suggesting the potential for independent regulation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Simon Lin, Subramanya Pandruvada, Hong Yu
Summary: Treatment with S1PR2 antagonist or S1PR2 shRNA enhanced osteogenesis in BMSCs via different signaling pathways, including increased vesicle trafficking-associated proteins and activation of Wnt/Ca2+ signaling, BMP/Smad signaling.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Marina C. Leite, Fabiana Galland, Maria Cristina Guerra, Leticia Rodrigues, Jessica Taday, Priscila T. Monteforte, Hanko Hirata, Carmem Gottfried, Rosario Donato, Soraya Smaili, Carlos-Alberto Goncalves
Summary: The secretion of S100B by astrocytes is triggered by the increase in intracellular Ca2+, which is caused by the mobilization of Ca2+ from the endoplasmic reticulum. The widely used solvent DMSO acts as a secretagogue for S100B, promoting its secretion both in vitro and in vivo.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Antoine Folcher, Dmitri Gordienko, Oksana Iamshanova, Alexandre Bokhobza, George Shapovalov, Dheeraj Kannancheri-Puthooru, Pascal Mariot, Laurent Allart, Emilie Desruelles, Corentin Spriet, Raquel Diez, Thibauld Oullier, Severine Marionneau-Lambot, Lucie Brisson, Sandra Geraci, Hathaichanok Impheng, V'yacheslav Lehen'kyi, Aurelien Haustrate, Adriana Mihalache, Pierre Gosset, Stephanie Chadet, Stephanie Retif, Maryline Laube, Julien Sobilo, Stephanie Lerondel, Giulia Villari, Guido Serini, Alessandra Fiorio Pla, Sebastien Roger, Gaelle Fromont-Hankard, Mustafa Djamgoz, Philippe Clezardin, Arnaud Monteil, Natalia Prevarskaya
Summary: There is increasing evidence that ion channels play a crucial role in the invasiveness and metastasis of cancer cells. However, the molecular mechanisms of ion signaling in promoting cancer behavior are not well understood, and the complex remodeling during metastasis remains to be explored. This study demonstrates that metastatic prostate cancer cells develop a specific Na+/Ca2+ signaling, which is essential for persistent invasion. The Na+ leak channel, NALCN, is identified as a major initiator and regulator of Ca2+ oscillations required for the formation of invadopodia. The signaling cascade involving NALCN, ion transport proteins, and proto-oncogene Src kinase promotes actin remodeling, secretion of proteolytic enzymes, and enhances cancer cell invasive potential and metastatic lesions in vivo.
Article
Multidisciplinary Sciences
Alessandro Moro, Anne van Nifterick, Ruud F. Toonen, Matthijs Verhage
Summary: This study identified dynamins as organizers of dense-core vesicle fusion sites in mammalian neurons, playing a crucial role in the release of neuropeptides and neuromodulators. Genetic or pharmacological inactivation of dynamins strongly impaired dense-core vesicle exocytosis, while synaptic vesicle exocytosis remained unaffected. The study concluded that mammalian dynamins regulate dense-core vesicle fusion sites by modulating the balance between fusogenic and non-fusogenic syntaxin-1, promoting its availability for SNARE complex formation and dense-core vesicle exocytosis.
Editorial Material
Microbiology
April DeMell, Savithramma P. Dinesh-Kumar
Summary: RNAi is an important plant antiviral strategy and its activation is triggered by wound-induced calcium signaling, as demonstrated by Wang et al. (2021) in Cell Host & Microbe. However, viral suppressors of RNAi interfere with calcium signaling components as a counter-defense mechanism.
CELL HOST & MICROBE
(2021)
Article
Multidisciplinary Sciences
Bijeta Prasai, Gideon J. Haber, Marie-Paule Strub, Regina Ahn, John A. Ciemniecki, Kem A. Sochacki, Justin W. Taraska
Summary: By utilizing correlative super-resolution light and electron microscopy, researchers have mapped the location and organization of Rab-GTPases and their effectors on exocytic vesicles, shedding light on their functional roles within cells.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Veronica Costiniti, Guilherme H. S. Bomfim, Maria Neginskaya, Ga-Yeon Son, Erna Mitaishvili, Marta Giacomello, Evgeny Pavlov, Rodrigo S. Lacruz
Summary: This study analyzed the functional features of mitochondria in rat ameloblasts and found an increased mitochondrial Ca2+ handling in the maturation stage, particularly in response to Ca2+ influx via store-operated Ca2+ entry (SOCE). These findings provide insights into the role of mitochondria in enamel and suggest that SOCE and mitochondrial Ca2+ uptake are complementary processes in biological mineralization.
Article
Chemistry, Multidisciplinary
Xiulan He, Andrew G. Ewing
Summary: In the presence of chaotropic anions, the opening of nanometer biological vesicles becomes a two-step process, allowing for independent counting of molecules in each subvesicular compartment. The study revealed a 68% catecholamine binding to the dense-core and differentiated two populations of large dense-core vesicles, with differences in biogenesis speculated to be caused by an increase in electrostatic attraction.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Review
Biochemistry & Molecular Biology
Axel T. Brunger, Jeremy Leitz
Summary: Synaptic neurotransmitter release involves a complex interplay of presynaptic proteins, including SNAREs, synaptotagmin, complexin, and other factors. This review focuses on the interactions between the SNARE complex and synaptotagmin, particularly the validated primary interface. The study also discusses other potential interfaces and presents new data on the tripartite interface and an inhibitor of the primary interface in a reconstituted system of synaptic vesicle fusion.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Blake H. Hummer, Theodore Carter, Breanna L. Sellers, Jenna D. Triplett, Cedric S. Asensio
Summary: This study reports the structure and function of HID-1 protein. The N-terminus of HID-1 mediates its binding with the cell membrane, while the C-terminus serves as the functional domain. A mutation can lead to loss of function.