Article
Pediatrics
Laura Gallardo-Alfaro, Patricia Lorente-Montalvo, Margarita Canellas, Eugenia Carandell, Antonio Oliver, Estrella Rojo, Beatriz Riera, Joan Llobera, Oana Bulilete, Alfonso Leiva, Anaida Obieta, Victoria Pascual, Pau Pericas, Carlos Raduan, Elsa Segura, Veronica Vega
Summary: A prospective diagnostic study was conducted in primary health care centers in Mallorca, Spain to evaluate the accuracy of the PanbioT Rapid Antigen Test for SARS-CoV-2. The study found that the test had higher sensitivity for patients referred by a general practitioner or pediatrician due to symptoms, compared to those who were asymptomatic or referred due to epidemiological exposure.
Article
Multidisciplinary Sciences
Marie Therese Ngo Nsoga, Ilona Kronig, Francisco Javier Perez Rodriguez, Pascale Sattonnet-Roche, Diogo Da Silva, Javan Helbling, Jilian A. Sacks, Margaretha de Vos, Erik Boehm, Angele Gayet-Ageron, Alice Berger, Frederique Jacquerioz-Bausch, Francois Chappuis, Laurent Kaiser, Manuel Schibler, Adriana Renzoni, Isabella Eckerle
Summary: In a high incidence setting in Switzerland, the study showed that using Panbio COVID-19 Ag Rapid Test on oropharyngeal swabs, if conducted by trained individuals and meeting quality standards, can meet the WHO criteria for Ag-RDTs (sensitivity >= 80% and specificity >= 97%).
Article
Virology
Felipe Perez-Garcia, Juan Romanyk, Pena Gomez-Herruz, Teresa Arroyo, Ramon Perez-Tanoira, Manuel Linares, Ines Perez Ranz, Andrea Labrador Ballestero, Helena Moya Gutierrez, Ma Jesus Ruiz-Alvarez, Juan Cuadros-Gonzalez
Summary: The study showed that both CerTest and Panbio Ag-RDTs demonstrated excellent specificity but lower sensitivity. Higher sensitivity was observed when Ct values were <25, while significantly lower sensitivity was noted with Ct >25. Samples taken within the first 5 days of symptom onset showed higher sensitivity for both tests. Both Ag-RDTs exhibited excellent agreement with each other and with PCR for samples with high viral loads or those taken within the first 5 days after symptom onset.
JOURNAL OF CLINICAL VIROLOGY
(2021)
Article
Virology
Reza Soleimani, Corentin Deckers, Te-Din Huang, Pierre Bogaerts, Stephanie Evrard, Isaline Wallemme, Boutaina Habib, Pauline Rouze, Olivier Denis
Summary: This study evaluated the performances of two rapid antigen testing devices in detecting SARS-CoV-2 in nasopharyngeal samples, showing relatively high sensitivity, especially with lower cycle threshold values, and 100% specificity for both devices.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Virology
Jean-Louis Bayart, Jonathan Degosserie, Julien Favresse, Constant Gillot, Marie Didembourg, Happy Phanio Djokoto, Valerie Verbelen, Gatien Roussel, Celine Maschietto, Francois Mullier, Jean-Michel Dogne, Jonathan Douxfils
Summary: This study evaluated the analytical performance of rapid antigen detection tests for the Delta and Omicron variants. The results showed poor performances for the rapidly antigen detection tests, especially for samples with low viral loads, particularly for the Omicron strain.
Review
Biology
Eleni Karlafti, Dimitrios Tsavdaris, Evangelia Kotzakioulafi, Georgia Kaiafa, Christos Savopoulos, Smaro Netta, Antonios Michalopoulos, Daniel Paramythiotis
Summary: Self-taken nasal rapid antigen tests have advantages such as rapid result reading and low cost. They have considerable specificity and some remarkable sensitivity. Therefore, they have a wide range of utility but cannot completely replace rt-PCR tests.
Review
Microbiology
Jia-Wen Xie, Yun He, Ya-Wen Zheng, Mao Wang, Yong Lin, Li-Rong Lin
Summary: This study assessed the diagnostic accuracy of rapid antigen tests (RAT) compared to RT-PCR for detecting SARS-CoV-2. The results showed that RAT had high sensitivity and specificity, especially in early infection and high viral load cases. Using nasal samples for antigen testing is a reliable alternative to nasopharyngeal sampling.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Microbiology
Michael Korenkov, Nareshkumar Poopalasingam, Matthias Madler, Kanika Vanshylla, Ralf Eggeling, Maike Wirtz, Irina Fish, Felix Dewald, Lutz Gieselmann, Clara Lehmann, Gerd Fatkenheuer, Henning Gruell, Nico Pfeifer, Eva Heger, Florian Klein
Summary: The study evaluated the performance of Standard Q COVID-19 Ag test in detecting SARS-CoV-2-infected individuals using RT-qPCR and virus culture. While the overall sensitivity of the RADT was low, it reliably detected patients with high RNA loads and identified individuals likely to transmit the virus. Negative RADT results corresponded with the lack of viral cultivability in Vero E6 cells, indicating that RADT can be a valuable tool for identifying individuals with high viral loads of SARS-CoV-2.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Editorial Material
Biochemistry & Molecular Biology
Stephen Bustin, Reinhold Mueller, Gregory Shipley, Tania Nolan
Summary: This commentary aims to clarify the true strengths and limitations of molecular testing technology, especially RT-qPCR, and to address and help stop the unfounded speculation surrounding its use.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Guojun Cao, Ke Lin, Jingwen Ai, Jianpeng Cai, Haocheng Zhang, Yiqi Yu, Qihui Liu, Xinyun Zhang, Yi Zhang, Zhangfan Fu, Jieyu Song, Hongyu Wang, Guanmin Yuan, Sen Wang, Ming Guan, Wenhong Zhang
Summary: LAMP-based virus nucleic acid rapid detection can effectively detect and isolate individuals infected with COVID-19 in the early stage, thereby preventing disease transmission.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Virology
Nareshkumar Poopalasingam, Michael Korenkov, Artem Ashurov, Janina Strobel, Irina Fish, Martin Hellmich, Henning Gruell, Clara Lehmann, Eva Heger, Florian Klein
Summary: This study evaluated the performance of the Standard Q COVID-19 Ag Test in detecting SARS-CoV-2 breakthrough infections in vaccinated individuals using RT-qPCR and rapid antigen detection testing. The results showed that RADT testing remains a valuable tool in detecting breakthrough infections with high viral RNA loads.
JOURNAL OF CLINICAL VIROLOGY
(2022)
Article
Multidisciplinary Sciences
Katarzyna Linkowska, Tomasz Bogiel, Katarzyna Lamperska, Andrzej Marszalek, Jaroslaw Starzynski, Lukasz Szylberg, Aleksandra Szwed-Kowalska, Malgorzata Pawlowska, Tomasz Grzybowski
Summary: Although SARS CoV-2 infection may not be as serious a threat as before, the increased transmissibility of the Omicron variant remains a challenge for health care systems. This study evaluates the performance of three SARS-CoV-2 diagnostic tests and finds that all have high specificity and sensitivity for detecting the virus. The LOD values for each test were determined and suggest that the reported outcomes should be based on internal validation rather than manufacturer's declarations.
SCIENTIFIC REPORTS
(2023)
Article
Immunology
Rebecca L. Smith, Laura L. Gibson, Pamela P. Martinez, Ruian Ke, Agha Mirza, Madison Conte, Nicholas Gallagher, Abigail Conte, Leyi Wang, Richard Fredrickson, Darci C. Edmonson, Melinda E. Baughman, Karen K. Chiu, Hannah Choi, Tor W. Jensen, Kevin R. Scardina, Shannon Bradley, Stacy L. Gloss, Crystal Reinhart, Jagadeesh Yedetore, Alyssa N. Owens, John Broach, Bruce Barton, Peter Lazar, Darcy Henness, Todd Young, Alastair Dunnett, Matthew L. Robinson, Heba H. Mostafa, Andrew Pekosz, Yukari C. Manabe, William J. Heetderks, David D. McManus, Christopher B. Brooke
Summary: This study found that RT-qPCR tests are more effective than antigen tests at identifying individuals infected with SARS-CoV-2 prior to or early during the infectious period. Additionally, serial testing multiple times per week can increase the sensitivity of antigen tests.
JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Virology
Xin-Xin Shen, Feng-Yu Li, Meng Qin, Guo-Hao Zhang, Meng-Yi Zhang, Hong Liu, Xiu-Li Sun, Zhen-Jiang Xin, Xue-Jun Ma
Summary: GeneClick is a device for nucleic acid self-testing of SARS-CoV-2, with three modules: a sampling kit, a microfluidic chip-based disposable cartridge, and an amplification reader. Clinical evaluation showed that GeneClick had a sensitivity and specificity of 97.93% and 99.72% respectively, outperforming antigen tests.
Article
Multidisciplinary Sciences
Lisa J. Krueger, Mary Gaeddert, Frank Tobian, Federica Lainati, Claudius Gottschalk, Julian A. F. Klein, Paul Schnitzler, Hans-Georg Kraeusslich, Olga Nikolai, Andreas K. Lindner, Frank P. Mockenhaupt, Joachim Seybold, Victor M. Corman, Christian Drosten, Nira R. Pollock, Britta Knorr, Andreas Welker, Margaretha de Vos, Jilian A. Sacks, Claudia M. Denkinger
Summary: The Abbott PanBio antigen-detecting rapid diagnostic test (Ag-RDT) demonstrated good performance for SARS-CoV-2 testing, with higher sensitivity observed in samples with Ct values <25 and within the first seven days of symptom onset. The test was also rated as easy to use and suitable for point-of-care settings, confirming its WHO recommendation for Emergency Use in resource-limited environments.