Article
Environmental Sciences
Weihua Fan, Jiaqi Jin, Zuotao Zhang, Lu Han, Keyuan Li, Chongyang Wang
Summary: The consortium 5H was found to have great potential for eliminating PAH pollution in saline/hypersaline environments, as it showed the ability to effectively degrade PAHs under different salinities, initial PAH concentrations, and pH conditions, while producing intermediates with low phytotoxicity.
ENVIRONMENTAL POLLUTION
(2022)
Article
Microbiology
Rui Chen, Zhenhua Zhao, Tao Xu, Xiaoqiang Jia
Summary: In this study, a highly efficient natural PHE-degrading microbial consortium HJ-SH was isolated from soil contaminated with PHE. The consortium showed a degradation efficiency higher than any other natural or engineered strains reported so far. Seven dominant strains were identified from the consortium, and one strain, SH-4, showed the strongest PHE degradation ability. The consortium exhibited high tolerance for PHE and was capable of degrading other organic pollutants as well. An artificial consortium HJ-7 was constructed, which outperformed the natural consortium HJ-SH in terms of reproducibility, application stability, and modification potential.
Article
Environmental Sciences
Surabhi Awasthi, Reshu Chauhan, Yuvraj Indoliya, Abhishek Singh Chauhan, ShashankKumar Mishra, Lalit Agrawal, Sanjay Dwivedi, Shiv Naresh Singh, Suchi Srivastava, Poonam C. Singh, Puneet Singh Chauhan, Debasis Chakrabarty, Sudhakar Srivastava, Rudra Deo Tripathi
Summary: This study investigated the mechanisms of microbial consortium-mediated growth promotion and arsenic amelioration in rice using transcriptome and proteome analysis. The results indicated that the microbial consortium significantly reduced arsenic toxicity, improved growth of root hairs, and affected the expression of various genes and proteins related to nutrient transport, defense, and metabolism in rice plants exposed to arsenic stress.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Engineering, Environmental
Longfei Jiang, Chunling Luo, Dayi Zhang, Mengke Song, Weiping Mei, Yingtao Sun, Gan Zhang
Summary: This study investigated the role of ryegrass in phenanthrene degradation in soils using DNA stable isotope probing (SIP) and metagenomics to unravel potential explanations for conflicting results in phytoremediation studies. The research found that ryegrass can enhance phenanthrene biodegradation efficiency and impact microbial community structure in the rhizosphere through carbohydrate metabolism, particularly fumaric acid. This suggests that rhizosphere effect on phenanthrene degradation efficiency may depend on the presence of active degraders with advantages in carbohydrate and fumaric acid metabolism.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Yinzhuang Zhu, Mingmei Ai, Xiaoqiang Jia
Summary: Through the design and construction of an artificial microbial consortium, this study successfully enhanced the production of mcl-PHA. A xylose-utilizing E. coli and a mcl-PHA-producing P. putida worked synergistically to achieve efficient accumulation of mcl-PHA.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Agricultural Engineering
Congfeng Xu, Xin Su, Jinghong Wang, Fangzheng Zhang, Guinan Shen, Yuan Yuan, Lei Yan, Hongzhi Tang, Fuqiang Song, Weidong Wang
Summary: The lignin-degrading bacterial consortium LDC efficiently degrades rice straw lignin in mineral salt medium. The microbial community structure and enzyme expression levels vary greatly during different incubation phases, with significant up-regulation of lignin degradation enzymes. This provides valuable microbial resources and data support for the industrialization of rice straw bio-pulping.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Lihua Zou, Shuiping Ouyang, Yueli Hu, Zhaojuan Zheng, Jia Ouyang
Summary: The study developed a synthetic microbial consortium consisting of engineered Pseudomonas putida and lactic acid production specialist Bacillus coagulans to produce lactic acid from highly toxic lignocellulosic hydrolysate. The microbial coculture system demonstrated strong potential for producing valuable products from lignocellulosic hydrolysates containing complex inhibitors by recruiting consortia of robust microorganisms with desirable characteristics.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Review
Biochemical Research Methods
Shawn Kulakowski, Deepanwita Banerjee, Corinne D. Scown, Aindrila Mukhopadhyay
Summary: Microbial bioconversion is an important approach to produce sustainable chemicals and commodities. However, in industrial-scale bioproduction, anaerobic conditions are preferred due to the high cost of oxygen transfer. Anaerobic bioconversion usually has limited substrate utilization, lower product yields, and reduced final product diversity compared to aerobic processes. This study introduces advances in microbial strain engineering, such as redox cofactor engineering, genome-scale metabolic modeling, and functional genomics, to improve bioproduction processes under low oxygen conditions and enable industrial-scale bioproduction systems.
CURRENT OPINION IN BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Ruolin Qin, Yinzhuang Zhu, Mingmei Ai, Xiaoqiang Jia
Summary: In recent years, microbial consortia composed of engineered Pseudomonas putida and Escherichia coli have become the focus of research for their high polymer yield and synthesis capacity in mcl-PHA production.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Asmaa Mawad, Hiba Albasri, Abdel-Gawad Shalkami, Saad Alamri, Mohamed Hashem
Summary: The study demonstrates that the constructed bacterial consortium showed higher efficiency in degrading phenanthrene compared to pure strains. Degradation rates were enhanced in the presence of intermediates pathway but reduced in the presence of catechol. Ring-cleavage dioxygenases were induced in all treatments.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2021)
Article
Biotechnology & Applied Microbiology
Patrick Bongartz, Isabel Bator, Kristina Baitalow, Robert Keller, Till Tiso, Lars Mathias Blank, Matthias Wessling
Summary: The bioeconomy plays a crucial role in mitigating greenhouse gas emissions and climate change, but faces limitations in industrialization due to economic and technical obstacles. This study presents a synergistic combination of simulations and experiments to design an efficient bubble-free aeration bioreactor.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Environmental Sciences
Qiang Pu, Xiao-Ting Fan, Hu Li, Xin-Li An, Simon Bo Lassen, Jian-Qiang Su
Summary: The study found that sub-inhibitory cadmium contamination significantly increased plasmid transfer frequency in aquatic bacterial communities, suggesting that cadmium may facilitate plasmid conjugation.
ENVIRONMENTAL POLLUTION
(2021)
Review
Microbiology
Dimple S. Pardhi, Rakeshkumar R. Panchal, Vikram H. Raval, Rushikesh G. Joshi, Peter Poczai, Waleed H. Almalki, Kiransinh N. Rajput
Summary: Microbial surfactants are amphiphilic substances that lower surface tension and have various characteristics, making them useful in industries like petroleum, cosmetics, and food. They are biodegradable and have low toxicity, making them environmentally friendly.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Patrick Bongartz, Tobias Karmainski, Moritz Meyer, John Linkhorst, Till Tiso, Lars M. Blank, Matthias Wessling
Summary: Bioreactors play a crucial role in the production of biopharmaceuticals, biomaterials, and sustainable substitutes for chemicals. However, the traditional aeration technique used in bioreactors has limitations such as shear stress, foaming, and sterility concerns. This study presents a membrane stirrer (MemStir) that combines simulations and experiments, providing efficient bubble-free aeration. The MemStir demonstrated excellent performance in a foam-free recombinant production process, delivering high oxygen transfer rates and demonstrating its potential for various applications including animal cell cultivation.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Rameshwar Tiwari, Chandran Sathesh-Prabu, Sung Kuk Lee
Summary: The present study describes the production of propionic acid (PA) using the microbial cell factory Pseudomonas putida EM42 and its ability to utilize levulinic acid (LA) derived from biomass. By engineering the strain and optimizing the bioprocess, the PA production was significantly improved, providing a new alternative route for sustainable and industrial production of PA.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)