Journal
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
Volume 10, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fbioe.2022.940511
Keywords
Zika virus; quantum dots (DQs); light-initiated cycloaddition; fluorescent probe; phenanthrenequinone
Funding
- National Natural Science Foundation of China [81972019, 21904145, 82002253, 81671970, 81772136, 82102444]
- Special Fund of Foshan Summit Plan [2020B019, 2020B012, 2020A015, 2019A006, 2019C002, 2019D008]
- Training Project of National Science Foundation for Outstanding/Excellent Young Scholars of Southern Medical University [C620PF0217]
- China Postdoctoral Science Foundation [2020M682783, 2021M693638]
- Regional Joint Fund of Natural Science Foundation of Guangdong Province [2020A1515110529]
- Guangdong Basic and Applied Basic Research Foundation [2020A1515010754]
- Medical Research Fund of Guangdong Province [A2020322]
- Special Fund for Science and Technology Innovation Strategy of Guangdong Province [2020A1515011402]
- Foundation of Foshan City [FS0AA-KJ218-1301-0034, 2018AB003411]
- Young Scientists Project of the National Key Research and Development Program [2021YFC2302200]
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The study introduced a photo-activated bio-orthogonal cycloaddition technique to construct a fluorogenic probe for labeling and visualizing ZIKV, which can effectively investigate the interaction between ZIKV and host cells.
Zika virus (ZIKV) is a re-emerging flavivirus that leads to devastating consequences for fetal development. It is crucial to visualize the pathogenicity activities of ZIKV ranging from infection pathways to immunity processes, but the accurate labeling of ZIKV remains challenging due to the lack of a reliable labeling technique. We introduce the photo-activated bio-orthogonal cycloaddition to construct a fluorogenic probe for the labeling and visualizing of ZIKV. Via a simple UV photoirradiation, the fluorogenic probes could be effectively labeled on the ZIKV. We demonstrated that it can be used for investigating the interaction between ZIKV and diverse cells and avoiding the autofluorescence phenomenon in traditional immunofluorescence assay. Thus, this bioorthogonal-enabled labeling strategy can serve as a promising approach to monitor and understand the interaction between the ZIKV and host cells.
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