Article
Oncology
George Sharbeen, Joshua A. McCarroll, Anouschka Akerman, Chantal Kopecky, Janet Youkhana, John Kokkinos, Jeff Holst, Cyrille Boyer, Mert Erkan, David Goldstein, Paul Timpson, Thomas R. Cox, Brooke A. Pereira, Jessica L. Chitty, Sigrid K. Fey, Arafath K. Najumudeen, Andrew D. Campbell, Owen J. Sansom, Rosa Mistica C. Ignacio, Stephanie Naim, Jie Liu, Nelson Russia, Julia Lee, Angela Chou, Amber Johns, Anthony J. Gill, Estrella Gonzales-Aloy, Val Gebski, Yi Fang Guan, Marina Pajic, Nigel Turner, Minoti Apte, Thomas P. Davis, Jennifer P. Morton, Koroush S. Haghighi, Jorjina Kasparian, Benjamin J. McLean, Yordanos F. Setargew, Phoebe A. Phillips
Summary: High expression of SLC7A11 in human PDAC tumor stroma, independently prognostic of poorer overall survival. The study demonstrates that PDAC-derived CAFs are highly dependent on SLC7A11 for cystine uptake and glutathione synthesis. Inhibition of SLC7A11 decreases CAF proliferation, reduces their resistance to oxidative stress, and inhibits their ability to support PDAC cell growth.
Article
Multidisciplinary Sciences
Gentaro Izumi, Hideki Nakano, Keiko Nakano, Gregory S. Whitehead, Sara A. Grimm, Michael B. Fessler, Peer W. Karmaus, Donald N. Cook
Summary: This study identified a subpopulation of cDCs in the lung of mice that can promote Th17 differentiation, showing a stepwise developmental progression from immature Ly-6C(+)Ly-6A/E+ cDC2 to mature cDC2. Through single cell RNA sequencing and adoptive transfer experiments, the researchers found that different stages of maturation and phenotype of mouse lung DC can promote Th17 or Th2 CD4(+) T cell differentiation.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Shan Huang, Qimin Zhang, Manyu Zhao, Xing Wang, Yilei Zhang, Boyi Gan, Peijing Zhang
Summary: The study reveals the role of ZRANB1 in ferroptosis resistance and uncovers the mechanisms involving modulation of E3 ligase activity through an unconventional catalytic domain.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Chen-chen Li, Hui-yan Chen, Xiliang Luo, Juan Hu, Chun-yang Zhang
Summary: The integration of multicolor fluorophore-encoded cascade signal amplification with single-molecule detection facilitates simultaneous measurement of different miRNAs in lung cancer tissues, demonstrating high sensitivity, good selectivity, and the capability of multiplexed assay. The method accurately quantifies miR-155 and miR-21, distinguishing their expression between non-small cell lung cancer patients and healthy individuals, validated by quantitative reverse transcription polymerase chain reaction.
Review
Oncology
Maozhen Peng, Ying Ying, Zheng Zhang, Liang Liu, Wenquan Wang
Summary: The reshaping of the pancreatic tumor microenvironment by chemotherapy, including the alteration of immune cells, pancreatic cancer cells, and cancer-associated fibroblast cells, has been studied quantitatively, functionally, and spatially. Understanding how chemotherapy reshapes the tumor microenvironment may lead to new therapeutic strategies to block its adverse tumor-promoting effects and prolong survival.
Article
Agriculture, Dairy & Animal Science
Chao Xu, Jing Shi, Rufeng Huang, Zhengchang Wu, Shenglong Wu, Wenbin Bao
Summary: This study investigates the role of lncRNA-mRNA in immune regulation by studying the transcriptome of Meishan pig spleen. A competing endogenous RNA network involving lncRNA-miRNA-mRNA was identified, which may play a critical role in immune regulation of Meishan pigs.
Article
Cell Biology
Youjing Yang, Yu Ma, Qianmin Li, Yi Ling, Yujia Zhou, Kaimiao Chu, Lian Xue, Shasha Tao
Summary: This study identified STAT6 as a critical regulator of epithelial ferroptosis during acute lung injury (ALI). STAT6 negatively regulates ferroptosis by competitively binding with CBP, inhibiting P53 acetylation and restoring SLC7A11 expression. Overexpression of STAT6 attenuated ALI-induced lung injury. STAT6 may be a potential therapeutic target for the treatment of ALI.
CELL DEATH & DISEASE
(2022)
Article
Multidisciplinary Sciences
Adolfo Flores-Saiffe Farias, Alejandro Chavez-Badiola, Gerardo Mendizabal-Ruiz, Roberto Valencia-Murillo, Andrew Drakeley, Jacques Cohen, Elizabeth Cardenas-Esparza
Summary: We propose the first automatic method for segmenting all morphological structures during the different developmental stages of the blastocyst. Our method is able to automatically segment blastocysts from different laboratory settings and developmental phases, increasing the knowledge base for embryo selection.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Jessica Pingel, Hans Martin Kjer, Fin Biering-Sorensen, Robert Feidenhans'l, Tim B. Dyrby
Summary: Synchrotron X-ray computed tomography (SXCT) allows for 3D imaging of tissue and enables the investigation of muscle fiber atrophy. The study examined healthy muscle fibers and muscle fibers at different stages of atrophy, revealing new insights into the micro-architecture of atrophied muscles. 3D analysis identified fiber splitting, fiber swelling, and fiber buckling, which could not be observed or quantified using 2D histological analysis.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Luhong Leng, Xiaoni Zhang, Weichao Liu, Zhiqiang Wu
Summary: Carnations are popular ornamental flowers with varied flower colors, which are mainly due to the accumulation of flavonoid compounds. MYB and bHLH transcription factors regulate the expression of anthocyanin biosynthetic genes. This study identified 106 MYB and 125 bHLH genes in the carnation genome and revealed key genes (DcaMYB13 and DcabHLH125) responsible for the formation of red petals in carnations. The findings provide valuable information for further studies on the regulation of anthocyanin biosynthesis in carnations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Kangli Wang, Rujia Dai, Yan Xia, Jianghua Tian, Chuan Jiao, Tatiana Mikhailova, Chunling Zhang, Chao Chen, Chunyu Liu
Summary: The study showed that DNA methylation and gene expression are coordinated across different human tissues and developmental stages, with correlations being tissue- and development-specific. These correlations are important for cellular functions, complex traits, and human diseases, highlighting the diverse roles of genetic and epigenetic factors in regulating gene expression.
Article
Multidisciplinary Sciences
A. Szostek-Mioduchowska, A. Wojtowicz, A. Sadowska, B. Moza Jalali, M. Slyszewska, K. Lukasik, A. Gurgul, T. Szmatola, M. Bugno-Poniewierska, G. Ferreira-Dias, D. J. Skarzynski
Summary: This study compares the transcriptome profiles of mare endometrium in different stages of endometrosis and identifies differentially expressed genes related to inflammation, metabolism, fibrosis, and cellular homeostasis. It also reveals the regulatory role of cytokines in cellular homeostasis, metabolism, and fibrosis signaling pathways. Furthermore, the study uncovers the importance of ADAMTS genes in the development of endometrosis and their response to cytokines in fibrotic endometrium and endometrial fibroblasts.
SCIENTIFIC REPORTS
(2023)
Article
Environmental Sciences
Rafiya Mushtaq, Mahinder Kumar Sharma, Javid Iqbal Mir, Sheikh Mansoor, Khalid Mushtaq, Simona Mariana Popescu, Abdul Raouf Malik, Hamed A. El-Serehy, Daniel Ingo Hefft, Sajad Ahmad Bhat, Sumati Narayan
Summary: Efficient irrigation management is crucial for plant growth and nutrient uptake. Drip irrigation at 100% ETc increased leaf area by 60% compared to rainfed conditions. Optimum irrigation levels significantly influence nutrient concentrations in leaf tissues.
Article
Environmental Sciences
Chuchu Xi, Xiaoqian Shi, Yujing Wang, Jing He, Shan Jiang, Bo Niu, Ying Chen, Fang Zhao, Zhengyu Cao
Summary: Perinatal exposure to bifenthrin (BF) has been found to affect neurodevelopment. This study investigated the susceptible time period for BF exposure and its possible mechanisms. The results showed that BF exposure during late gestation caused neurobehavioral deficits in offspring, but these deficits remitted with age. The study also revealed that altered protein levels of VGluT1, NR1, NR2A, NR2B, and VGAT1, as well as increased gene expression levels of Il-1β, Il-6, and Tnf-α, were associated with BF exposure-induced neurobehavioral impairments.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Plant Sciences
Feng Xiao, Yang Zhao, Xiurong Wang, Yanan Sun
Summary: This study investigated the growth and development of G. sinensis thorn (Zao Jiao Ci) by measuring the content of soluble sugar and starch and performing transcriptome sequencing. The results showed changes in the sugar and starch content during thorn formation, and identified several genes that may be related to thorn maintenance and growth. Gene Ontology enrichment analysis revealed terms related to development regulation and photomorphogenesis. The study also validated the transcriptome data using real-time quantitative PCR. Overall, this research provides a comprehensive understanding of G. sinensis thorn development.