PARA: A New Platform for the Rapid Assembly of gRNA Arrays for Multiplexed CRISPR Technologies

Expanding the scope of plant genome engineering with Cas12a orthologs and highly multiplexable editing systems

(2021) Yingxiao Zhang et al.   Nature Communications

Construct design for CRISPR/Cas-based genome editing in plants

(2021) Md Mahmudul Hassan et al.   TRENDS IN PLANT SCIENCE

Multiplexed CRISPR technologies for gene editing and transcriptional regulation

(2020) Nicholas S. McCarty et al.   Nature Communications

Enabling one-pot Golden Gate assemblies of unprecedented complexity using data-optimized assembly design

(2020) John M. Pryor et al.   PLoS One

A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae

(2019) Yueping Zhang et al.   Nature Communications

CHOPCHOP v3: expanding the CRISPR web toolbox beyond genome editing

(2019) Kornel Labun et al.   NUCLEIC ACIDS RESEARCH

Highly multiplexed genome engineering using CRISPR/Cas9 gRNA arrays

(2018) Morito Kurata et al.   PLoS One

Comprehensive Profiling of Four Base Overhang Ligation Fidelity by T4 DNA Ligase and Application to DNA Assembly

(2018) Vladimir Potapov et al.   ACS Synthetic Biology

A multi-purpose toolkit to enable advanced genome engineering in plants

(2017) Tomas Cermak et al.   PLANT CELL

Multiplexed CRISPR/Cas9 Genome Editing and Gene Regulation Using Csy4 in Saccharomyces cerevisiae

(2017) Raphael Ferreira et al.   ACS Synthetic Biology

Augmenting CRISPR applications in Drosophila with tRNA-flanked sgRNAs

(2016) Fillip Port et al.   NATURE METHODS

Dimeric CRISPR RNA-Guided FokI-dCas9 Nucleases Directed by Truncated gRNAs for Highly Specific Genome Editing

(2015) Nicolas Wyvekens et al.   HUMAN GENE THERAPY

A versatile modular vector system for rapid combinatorial mammalian genetics

(2015) Joachim Albers et al.   JOURNAL OF CLINICAL INVESTIGATION

Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system

(2015) Kabin Xie et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A CRISPR/Cas9 toolkit for multiplex genome editing in plants

(2014) Hui-Li Xing et al.   BMC PLANT BIOLOGY

Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector

(2014) Ami M. Kabadi et al.   NUCLEIC ACIDS RESEARCH

Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system

(2014) Tetsushi Sakuma et al.   Scientific Reports

Self-processing of ribozyme-flanked RNAs into guide RNAsin vitroandin vivofor CRISPR-mediated genome editing

(2013) Yangbin Gao et al.   Journal of Integrative Plant Biology

Phytozome: a comparative platform for green plant genomics

(2011) David M. Goodstein et al.   NUCLEIC ACIDS RESEARCH