Article
Biotechnology & Applied Microbiology
Hanxin Xu, Fen Xu, Jiajia Zhao, Caixia Zhou, Jiarong Liu
Summary: The study demonstrates that autologous platelet-rich plasma (PRP) can enhance migration, proliferation, and osteogenic differentiation of dental pulp cells, and this process is strengthened by activating autophagy. Inhibiting autophagy weakens the effects of PRP on cells, while an autophagy activator enhances the promoting effects of PRP.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Applied
Amirabbas Rastegar, Mahboobeh Mahmoodi, Mohammad Mirjalili, Navid Nasirizadeh
Summary: The fabrication of PCL/CS-PRF core-shell nanofibrous scaffold via coaxial electrospinning showed improved mechanical properties and sustained release of PRF. In vitro experiments demonstrated higher ALP activity, calcium deposition, and osteogenic differentiation on the scaffold containing PRF. This suggests that PCL/CS-PRF core-shell scaffolds have potential for bone tissue engineering applications.
CARBOHYDRATE POLYMERS
(2021)
Article
Biotechnology & Applied Microbiology
Ayano Hatori, Daiki Yamakawa, Sarah Al-Maawi, Eva Dohle, Jin Chikira, Yasuyuki Fujii, Megumu Miki, Robert Sader, Daichi Chikazu, Shahram Ghanaati, Yoko Kawase-Koga
Summary: This study investigates the use of PRF-conditioned medium (CM) in enhancing the osteogenic potential of DPSCs. Compared to FBS culture, DPSCs cultured with PRF-CM exhibit superior calcification levels but inferior proliferation rates.
BIOENGINEERING-BASEL
(2023)
Article
Biochemistry & Molecular Biology
Zahra Kargarpour, Jila Nasirzade, Layla Panahipour, Richard J. Miron, Reinhard Gruber
Summary: The study reveals that PRF lysates exhibit potent anti-inflammatory activity in murine mesenchymal cells, reducing cytokine-induced expression of inflammatory mediators and inflammatory response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Maryam Bidar, Aminmohammad Bahlakeh, Mahmoud Mahmoudi, Farzaneh Ahrari, Reza Shahmohammadi, Hamid Jafarzadeh
Summary: This study evaluated the effect of platelet-rich plasma (PRP) and GaAlAs laser on proliferation and differentiation of human dental pulp stem cells. The results showed that laser irradiation could induce pulp cell proliferation and demonstrated a better performance than PRP in this regard.
LASERS IN MEDICAL SCIENCE
(2021)
Article
Dermatology
Tahereh Haghparast-Kenarsari, Majid Abdouss, Saeed Heidari Keshel, Zoheir Heshmatipour, Azam Rahimi, Esmaeil Biazar
Summary: Tannic acid was used as a green cross-linker to improve the properties of platelet-rich fibrin (PRF). The results showed that increasing the cross-linker concentration led to changes in scaffold structure, decreased degradation rate, and increased mechanical strength. The cross-linked scaffolds exhibited good biocompatibility and antibacterial effect.
INTERNATIONAL WOUND JOURNAL
(2023)
Article
Chemistry, Medicinal
Yulla Klinger de Carvalho Leite, Antonia Carla de Jesus Oliveira, Patrick Veras Quelemes, Napoleao Martins Argolo Neto, Camila Ernanda Sousa de Carvalho, Huanna Waleska Soares Rodrigues, Michel Mualem de Moraes Alves, Fernando Aecio de Amorim Carvalho, Daniel Dias Rufino Arcanjo, Edson Cavalcanti da Silva-Filho, Alessandra Durazzo, Massimo Lucarini, Maria Acelina Martins de Carvalho, Durcilene Alves da Silva, Jose Roberto de Souza de Almeida Leite
Summary: In this study, a scaffold based on chitosan and phthalated cashew tree gum was developed and tested as a support for the growth of human mesenchymal stem cells. The scaffold showed high swelling rate and appropriate pore sizes analyzed by scanning electron microscopy. Cells seeded on the scaffold exhibited typical morphology, attachment, and adequate distribution inside the matrix pores.
Article
Cell & Tissue Engineering
Jian Zhang, Jiankun Wu, Xiuya Lin, Xueli Liu
Summary: This study found that platelet-rich fibrin (PRF) can promote the proliferation and osteo-/odontoblastic differentiation of human dental pulp stem cells (hDPSCs), possibly through the Notch signaling pathway.
CURRENT STEM CELL RESEARCH & THERAPY
(2023)
Article
Biochemistry & Molecular Biology
Cristina- Bucchi, Ella Ohlsson, Josep Maria de Anta, Melanie Woelflick, Kerstin Galler, Maria Cristina Manzanares-Cespedes, Matthias Widbiller
Summary: Pluripotent stem cells derived from the amnion show the ability to adhere to dentin and mineralize in vitro, but exhibit unfavorable proliferation behavior and fail to undergo odontoblastic transition.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Physical
Wei Yin, Gaoxia Liu, Jinhong Li, Zhuan Bian
Summary: The study revealed various cell populations in human dental pulp, including dental pulp stem cells, T cells, macrophages, etc., which support each other through growth signals. Pulp cells have the greatest communication with other cell types, while T cells have the least. T cells and endothelial cells play a role in monitoring bacterial invasion through specific receptor expressions. These findings provide insights into the cellular composition of normal dental pulp.
Article
Biotechnology & Applied Microbiology
Tung Nguyen-Thanh, Bao-Song Nguyen-Tran, Sara Cruciani, Thuy-Duong Nguyen-Thi, Thuan Dang-Cong, Margherita Maioli
Summary: This study examined the effects of platelet-rich fibrin (PRF) scaffolds and autologous uncultured bone marrow mononuclear cells on osteochondral regeneration in rabbits. The histological characteristics of the PRF scaffolds were assessed, and the results showed that the combination of autologous PRF and uncultured bone marrow mononuclear cells promoted the healing of articular cartilage and subchondral bone in the rabbit knees.
BIOENGINEERING-BASEL
(2023)
Article
Biochemistry & Molecular Biology
K. Sarath Kumar, Selvakumar Kritika, Natesan Sundramurthy Karthikeyan, Venkatappan Sujatha, Sekar Mahalaxmi, Cingaram Ravichandran
Summary: The aim of this study was to compare chitosan and cobalt incorporated chitosan scaffold in terms of material characteristics, cytotoxicity, and cell adhesion potential. The results showed that cobalt incorporated chitosan scaffold had an amorphous, porous surface and formed chelation with chitosan. XTT analysis demonstrated that the experimental scaffold was non-cytotoxic. Cell seeding assay revealed enhanced cell adhesion to the cobalt incorporated chitosan scaffold at a 1:1 ratio of 100 mL of 100 mu mol/L cobalt chloride solution in 100 mL of 2% chitosan solution.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Cell Biology
Edward J. Calabrese, Evgenios Agathokleous, Gaurav Dhawan, Rachna Kapoor, Vittorio Calabrese
Summary: This study represents the first assessment of hormetic dose responses by human dental pulp stem cells, focusing on cell renewal and differentiation. The study found that hormetic dose responses are commonly observed in this model, involving a wide range of chemicals including pharmaceuticals, dietary supplements/extracts from medicinal plants, and endogenous agents. The study evaluated the mechanistic foundations, study design considerations, and therapeutic implications of the hormetic dose responses in hDPSCs.
AGEING RESEARCH REVIEWS
(2022)
Article
Cell Biology
Zahra Kargarpour, Layla Panahipour, Michael Mildner, Richard J. Miron, Reinhard Gruber
Summary: Platelet-rich fibrin (PRF) has potent anti-inflammatory activity, however, the components responsible for this effect are unknown. In this study, we investigated whether the lipid fraction of PRF also exhibits anti-inflammatory properties. Our results show that lipids from PRF can significantly reduce the inflammatory response of ST2 bone marrow stromal cells and primary bone marrow macrophages. These findings suggest that the lipid fraction is at least partially responsible for the anti-inflammatory activity of PRF in vitro.
Article
Polymer Science
Reza Samanipour, Sina Farzaneh, Javad Ranjbari, Sheida Hashemi, Arash Khojasteh, Simzar Hosseinzadeh
Summary: In this study, an oxygen-releasing PCL/SP-PVA scaffold was developed and used as a platform for osteogenic differentiation of dental pulp stem cells. The scaffold showed no inhibitory effect on cell growth and provided a suitable matrix for osteogenic differentiation. The results support the use of oxygen-releasing scaffolds as a potential strategy to accelerate bone regeneration.
Article
Dentistry, Oral Surgery & Medicine
Wen-Jin Chen, Jing Xie, Xi Lin, Ming-Hang Ou, Jun Zhou, Xiao-Lang Wei, Wen-Xia Chen
Summary: The study demonstrated that LPS-preconditioned sEVs can enhance the proliferation, migration, angiogenesis, and differentiation of mesenchymal stem cells, leading to structurally improved regenerated tissue similar to normal dental pulp in vivo. These findings suggest that LPS-preconditioned sEVs play a significant role in dental pulp regeneration.
JOURNAL OF ENDODONTICS
(2021)
Article
Dentistry, Oral Surgery & Medicine
Hejia Qin, Jindong Long, Jun Zhou, Liuxian Wu, Fangfang Xie
Summary: The study successfully synthesized phosphate-terminated polyamidoamine dendrimer (PAMAM-PO3H2) and demonstrated their potential as analogs of NCPs for repairing etched dentin. The combination of PAMAM-PO3H2 and PAMAM-COOH shows promise as a feasible therapeutic method for treating dentin caries and dentin hypersensitivity, as indicated by various characterization techniques.
DENTAL MATERIALS JOURNAL
(2021)
Article
Chemistry, Analytical
Feng Luo, Jun Zhou, Kangjing Li, Xinglu Jiang
Summary: This study develops a novel contrast agent for noninvasive gastrointestinal tract imaging to detect pH change. The agent, composed of gold nanoclusters and diatrizoic acid, is highly sensitive to acid-base response. The study demonstrates that the agent can detect pH change in the gastrointestinal tract through optical/CT double-modal imaging.
ANALYTICA CHIMICA ACTA
(2022)
Article
Dentistry, Oral Surgery & Medicine
Xiao-Lang Wei, Ling Luo, Meng-zhu Chen, Jun Zhou, Bin-Yun Lan, Xue-Meng Ma, Wen-Xia Chen
Summary: This study investigated the expression and role of Substance P (SP) in reparative dentinogenesis. It was found that SP contributes to odontoblastic differentiation by binding to the neurokinin-1 receptor (NK1R). These findings provide important insights into the process of reparative dentin formation.
JOURNAL OF ENDODONTICS
(2023)
Article
Anatomy & Morphology
Jun Zhou, Ming-Hang Ou, Xiao-Lang Wei, Bin-Yuan Lan, Wen-Jin Chen, Si-Jia Song, Wen-Xia Chen
Summary: This study investigated the effects of different phenotype macrophages on dental pulp regeneration. M2 macrophages were found to promote the proliferation, migration, and osteogenic differentiation of hDPSCs, while M1 macrophages led to calcium salt deposition and disorganization of collagen fibers.
Article
Chemistry, Physical
Jun Zhou, Kangjing Li, Hejia Qin, Beibei Xie, Haiqin Liao, Xiaoping Su, Cuiping Li, Xuan He, Wenxia Chen, Xinglu Jiang
Summary: Programmed response, carrier-free, and multimodal therapy drug delivery systems (DDS) offer promising solutions to address multidirectional cytotoxic effects, inefficient antitumor effects, and severe side effects in cancer therapy. In this study, a carrier-free DDS called IFNa1b-ICG-DOX (IID) was developed using a one-step method without any additional reagents, combining interferon a1b (IFNa1b), indocyanine green (ICG), and doxorubicin (DOX). The system achieved smart and programmed drug delivery through stimuli-responsive controlled release triggered by low pH and near-infrared (NIR) light. In vivo, the larger size and positive charge of IID in low pH environments facilitated its accumulation in tumor tissue, and NIR light could be used to control the release of the drugs, enabling efficient combined chemotherapy, photothermal therapy, and immunotherapy.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
