Article
Cell Biology
Won-Ki Pang, Yoo-Jin Park, Myung-Geol Pang
Summary: This study investigated the potential of sperm RNA markers in assessing various aspects of sperm function and identified genes related to male fertility. The findings provide valuable insights into male infertility.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ana Sanchez-Rodriguez, Ester Sansegundo, Maximiliano Tourmente, Eduardo R. S. Roldan
Summary: Viscosity affects sperm ATP levels and kinematics during capacitation in vitro, with different responses observed in different mouse species.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Joao C. Ribeiro, Raquel L. L. Bernardino, Ana Goncalves, Alberto Barros, Giuseppe Calamita, Marco G. G. Alves, Pedro F. F. Oliveira
Summary: Osmoregulation is essential for sperm function, and aquaglyceroporins play a crucial role in glycerol permeability, which is important for sperm motility. AQP7 is especially significant in glycerol transport, and its impairment may lead to reduced sperm motility in asthenozoospermic individuals.
Article
Biochemistry & Molecular Biology
Fanwen Zeng, Mingming Wang, Ju Li, Chengde Li, Xueqing Pan, Li Meng, Li Li, Hengxi Wei, Shouquan Zhang
Summary: In this study, we found that porcine beta-defensin 129 (pBD129) is widely present in accessory sex glands and coats the surface of sperm. Inhibiting pBD129 on the sperm surface resulted in increased acrosome integrity, tyrosine phosphorylation levels, and Ca2+ influx, leading to a reduced in vitro fertilization cleavage rate. Furthermore, treatment with recombinant pBD129 restored sperm motility in semen contaminated with Escherichia coli.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Reproductive Biology
Naomi C. Bernecic, Simon P. de Graaf, Tamara Leahy, Bart M. Gadella
Summary: This study reveals that both sheep serum and HDLs can induce cholesterol efflux in ram spermatozoa, with HDLs also triggering hyperactivated motility. Inhibition of ABCA1 and SR-BI function only marginally reduces cholesterol efflux, suggesting a specific facilitated pathway for cholesterol efflux in ram spermatozoa. These findings shed light on the complexity of events leading to fertilization and suggest the potential application of HDL supplementation in IVF media.
BIOLOGY OF REPRODUCTION
(2021)
Article
Cell Biology
Mina Pencheva, Donka Keskinova, Pavel Rashev, Yvetta Koeva, Nina Atanassova
Summary: Testicular angiotensin converting enzyme (ACE) plays a crucial role in male reproduction and fertility. Research shows that the proper distribution of tACE in the neck and mid-piece of sperm is essential for normal sperm motility. Different patterns of tACE expression were observed in pathological semen samples compared to normospermia.
Article
Pharmacology & Pharmacy
Jacob Ferreira, Lonny R. Levin, Jochen Buck
Summary: This article discusses the 10 isoforms of adenylyl cyclase that produce the second messenger cyclic AMP in humans, including nine G protein-coupled transmembrane adenylyl cyclases (tmACs; ADCY1-9) and one bicarbonate-regulated soluble adenylyl cyclase (sAC; ADCY10). It highlights the unique druggability of sAC and outlines approaches to target sAC for novel forms of male and female contraception.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ana Sanchez-Rodriguez, Ingrid I. D. Idrovo, Juan Antonio Rielo, Eduardo R. S. Roldan
Summary: This study aimed to analyze changes in spermatozoa of Phodopus hamsters under different incubation conditions. The results showed that different media affected sperm motility, viability, capacitation patterns, and kinematics. Bicarbonate was found to have a role in promoting capacitation, but progesterone did not enhance the percentage of capacitated cells. The study provides insights into the changes associated with capacitation and hyperactivated motility in Phodopus species, but further research is needed to explore optimal media conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Shengnan Cong, Jingjing Zhang, Feng Pan, Lianjun Pan, Aixia Zhang, Jiehua Ma
Summary: Ion channels in the sperm tail, such as CatSper and KSper, play a crucial role in triggering essential physiological reactions necessary for successful fertilization, like progressive motility and hyperactivation. Other ion channels like Hv1, sNHE, ENaC, TRP channels, and CFTR are also found in the flagellum. This review discusses the recent advancements in these ion channels' understanding in the flagellum, their impact on sperm function, and the interplay between them through factors like PH, membrane potential, and cAMP. These ion channels may have potential clinical applications in treating infertility.
Article
Developmental Biology
Fang Yang, Maria Gracia Gervasi, Gerardo Orta, Darya A. Tourzani, Jose Luis De La Vega-Beltran, Gordon Ruthel, Alberto Darszon, Pablo E. Visconti, P. Jeremy Wang
Summary: The CatSper cation channel is essential for sperm capacitation and male fertility. The study identifies C2CD6 as a subunit of the CatSper complex and demonstrates its role in the formation of calcium signaling nanodomains on flagellar membranes. Deficiency of C2CD6 leads to male sterility due to depleted CatSper nanodomains and impaired sperm function. C2CD6 may facilitate the incorporation of CatSper complex into the flagellar plasma membrane and function as a calcium sensor.
Article
Cell Biology
Juan J. Ferreira, Pascale Lybaert, Lis C. Puga-Molina, Celia M. Santi
Summary: Our study shows that CatSper channels in mouse and human sperm are activated through alkaline conditions and the presence of HCO3-, leading to an influx of Ca2+. Furthermore, HCO3- activates the soluble adenylyl cyclase/protein kinase A pathway, which sensitizes CatSper channels to respond to membrane depolarization, allowing for Ca2+ influx.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Agriculture, Dairy & Animal Science
Dilip Kumar Swain, Pratishtha Sharma, Nadeem Shah, Manisha Sethi, Abhishek Mahajan, Shashikant Gupta, Abhishek Kumar Mishra, Sarvajeet Yadav
Summary: Sperm motility is regulated by complex signaling and communication mechanisms, in which regulatory membrane proteins called sperm ion channels play important roles. Ion channels are involved in the regulation of sperm motility, energy homeostasis, membrane integrity, and fertilization processes.
ANIMAL REPRODUCTION SCIENCE
(2022)
Article
Cell Biology
Marie Bisconti, Baptiste Leroy, Meurig T. T. Gallagher, Coralie Senet, Baptiste Martinet, Vanessa Arcolia, Ruddy Wattiez, Jackson C. C. Kirkman-Brown, Jean-Francois Simon, Elise Hennebert
Summary: This study investigated the presence and activity of two types of ribosomes in mature human spermatozoa. The results showed that both types of ribosomes are localized in different parts of the spermatozoa, and inhibition of mitochondrial ribosomes leads to decreased sperm motility.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Medicine, General & Internal
Rossella Cannarella, Rosita A. Condorelli, Carmelo Gusmano, Nunziata Barone, Nunziatina Burrello, Antonio Aversa, Aldo E. Calogero, Sandro La Vignera
Summary: The study observed a slight decrease in total sperm count and percentage of spermatozoa with normal morphology, while a significant increase in progressive sperm motility was found in men from Eastern Sicily between 2011 and 2020. The total sperm count was significantly lower and a trend of increasing progressive sperm motility was noticed in the quinquennium 2016-2020 compared to 2011-2015. The city of residence did not have a significant influence on sperm parameters.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Olivia Petrone, Steven Serafini, Bess Yi Kun Yu, Valeriy Filonenko, Ivan Gout, Cristian O'Flaherty
Summary: Spermatozoa are susceptible to oxidative stress due to limited antioxidant defences, high polyunsaturated fatty acids content, and inability to synthesize proteins. Reactive oxygen species (ROS) harm spermatozoa, causing oxidative damage to lipids, proteins, and DNA, leading to infertility. Coenzyme A (CoA) functions as a major cellular antioxidant through covalent modification of cysteines (protein CoAlation). This study found that hydrogen peroxide and diamide produced the highest levels of protein CoAlation, reducing sperm motility without impairing viability. Protein CoAlation levels are regulated by 2-Cys peroxiredoxins (PRDXs) and are lower in capacitated spermatozoa. This study reveals the role of PRDXs in regulating protein CoAlation, which contributes to the antioxidant response and redox regulation during sperm capacitation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Irene Sanchez-Ajofrin, Maria Iniesta-Cuerda, Maria Jesus Sanchez-Calabuig, Patricia Peris-Frau, Alicia Martin-Maestro, Jose Antonio Ortiz, Maria Del Rocio Fernandez-Santos, Jose Julian Garde, Alfonso Gutierrez-Adan, Ana Josefa Soler
ANIMAL REPRODUCTION SCIENCE
(2020)
Review
Biochemistry & Molecular Biology
Patricia Peris-Frau, Ana Josefa Soler, Maria Iniesta-Cuerda, Alicia Martin-Maestro, Irene Sanchez-Ajofrin, Daniela Alejandra Medina-Chavez, Maria Rocio Fernandez-Santos, Olga Garcia-Alvarez, Alejandro Maroto-Morales, Vidal Montoro, J. Julian Garde
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Agriculture, Dairy & Animal Science
Beatriz Cardoso, Irene Sanchez-Ajofrin, Cristina Castano, Olga Garcia-Alvarez, Milagros Cristina Esteso, Alejandro Maroto-Morales, Maria Iniesta-Cuerda, Jose Julian Garde, Julian Santiago-Moreno, Ana Josefa Soler
Article
Agriculture, Dairy & Animal Science
Irene Sanchez-Ajofrin, Maria Iniesta-Cuerda, Patricia Peris-Frau, Alicia Martin-Maestro, Daniela-Alejandra Medina-Chavez, Carolina Maside, Maria Rocio Fernandez-Santos, Jose Antonio Ortiz, Vidal Montoro, Jose Julian Garde, Ana Josefa Soler
Article
Andrology
Patricia Peris-Frau, Manuel alvarez-Rodriguez, Alicia Martin-Maestro, Maria Iniesta-Cuerda, Irene Sanchez-Ajofrin, Daniela Alejandra Medina-Chavez, Jose Julian Garde, Margarita Villar, Heriberto Rodriguez-Martinez, Ana Josefa Soler
Summary: The study revealed that sperm chromatin condensation decreased gradually during in vitro capacitation of ram spermatozoa, while sperm DNA integrity remained intact. Such changes in chromatin condensation appeared faster after sperm cryopreservation.
Article
Agriculture, Dairy & Animal Science
Jose Antonio Ortiz, Olga Garcia-alvarez, Mariano Amo-Salas, Alejandro Maroto-Morales, Maria Iniesta-Cuerda, Maria del Rocio Fernandez-Santos, Ana Josefa Soler, Jose Julian Garde
Summary: The study found that melatonin implants in yearling Iberian red deer hinds significantly improved their reproductive performance, increased calves' weight, advanced calving date, and had a positive impact on the reproductive outcomes of yearling hinds with low liveweight.
Article
Biochemistry & Molecular Biology
Alejandro Jurado-Campos, Pedro Javier Soria-Meneses, Francisca Sanchez-Rubio, Enrique Niza, Ivan Bravo, Carlos Alonso-Moreno, Maria Arenas-Moreira, Olga Garcia-alvarez, Ana Josefa Soler, Jose Julian Garde, Maria del Rocio Fernandez-Santos
Summary: The use of hydrogel and nanoemulsion carriers for vitamin E showed promising results in preventing oxidative stress in sperm cells, maintaining sperm viability and kinematic parameters. This new formulation of vitamin E could be a significant approach for preserving sperm physiology and protecting against oxidative damage.
Article
Biology
Patricia Peris-Frau, Irene Sanchez-Ajofrin, Alicia Martin Maestro, Carolina Maside, Daniela Alejandra Medina-Chavez, Olga Garcia-alvarez, Maria del Rocio Fernandez-Santos, Vidal Montoro, Jose Julian Garde, Manuel Ramon, Ana Josefa Soler
Summary: Spermatozoa go through diverse changes to achieve fertilizing potential and specific motility pattern. Cryopreservation alters the distribution of motile sperm subpopulations, affecting sperm quality. Changes in sperm motility and tyrosine phosphorylation key events during capacitation can be modified by the cryopreservation process.
Article
Agriculture, Dairy & Animal Science
Daniela Alejandra Medina-Chavez, Ana Josefa Soler, Alicia Martin-Maestro, Silvia Villaverde, Irene Sanchez-Ajofrin, Patricia Peris-Frau, Enrique del Olmo, Alfonso Bisbal, Olga Garcia-Alvarez, Maria del Rocio Fernandez-Santos, Jose Julian Garde
Summary: The study evaluated different methods of storing and freezing Iberian red deer epididymal sperm under field conditions. Samples stored in straws during refrigeration showed better sperm quality, with active mitochondria and lower apoptotic cells. Freezing in liquid nitrogen vapors in a tank or box yielded better results than freezing in dry ice or over a metallic plate.
