Journal
PROGRESS IN NATURAL SCIENCE-MATERIALS INTERNATIONAL
Volume 32, Issue 4, Pages 443-455Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.pnsc.2022.06.006
Keywords
Flax protein; Silk fibroin; 8-TCP; Electrospinning; Docking; Bone regeneration
Funding
- DST, New Delhi, India [EMR/2016/002562]
- Indian Council of Medical Research [45/39/2020-/BIO/BMS]
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A novel dual protein-based composite nanofiber scaffold was developed to promote cartilage tissue regeneration. Experimental results demonstrated improved cell adhesion and proliferation, as well as good biological compatibility and antibacterial properties.
The process of bone tissue regeneration involves an intricate network of a biological macromolecules that includes proteins and peptides to support stimulation and healing response. Here, we have fabricated a novel flaxseed/silk fibroin based (dual protein) composite nanofibers using 8-TCP to improve the biological properties and promote cartilage tissue regeneration along with a controllable rate of biodegradation. The physiochemical properties of PVA/8-TCP/FP:SF were characterized by FTIR, XRD, SEM, porosity, contact angle measurements and mechanical evaluation etc. This study demonstrates the biological performance of the developed nanofibers by using hemocompatibility, biodegradation ability, and apatite deposition in stimulated body fluid. Moreover, the cell viability assay was performed on MG-63 osteoblast cells and long-term antibacterial rate against E. coli and S. aureus was analysed. Docking results of flax protein and silk fibroin protein further confirmed the stable modes of intramolecular bonding and the most stable electrostatic values using theoretical calculations. Taken together, the experimental results proved that the dual protein-based fibrous scaffold was found to be up-regulated in terms of biological activity with enhanced cell adhesion and proliferation.
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