Journal
JOURNAL OF DENTAL RESEARCH
Volume 96, Issue 3, Pages 323-330Publisher
SAGE PUBLICATIONS INC
DOI: 10.1177/0022034516678208
Keywords
odontoblasts; pulp biology; dentinogenesis; reparative dentin; stem cells; dentin sialophosphoprotein
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Funding
- National Institutes of Health (National Institute of Dental and Craniofacial Research) [R01-DE016689, R01-AR055607, R90-DE022526]
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The goal of this study was to examine the contribution of perivascular cells to odontoblasts during the development, growth, and repair of dentin using mouse molars as a model. We used an inducible, Cre-loxP in vivo fate-mapping approach to examine the contributions of the descendants of cells expressing the alpha SMA-CreERT2 transgene to the odontoblast lineage. In vivo lineage-tracing experiments in molars showed the contribution of alpha SMA-tdTomato(+) cells to a small number of newly formed odontoblasts during primary dentinogenesis. Using an experimental pulp exposure model in molars to induce reparative dentinogenesis, we demonstrate the contribution of alpha SMA-tdTomato(+) cells to cells secreting reparative dentin. Our results demonstrate that alpha SMA-tdTomato(+) cells differentiated into Col2.3-GFP(+) cells composed of both Dspp(+) odontoblasts and Bsp+ osteoblasts. Our findings identify a population of mesenchymal progenitor cells capable of giving rise to a second generation of odontoblasts during reparative dentinogenesis. This population also makes a small contribution to odontoblasts during primary dentinogenesis.
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