Review
Chemistry, Physical
Necdet Adanir, Zohaib Khurshid, Jithendra Ratnayake
Summary: This systematic review evaluates the effectiveness of decellularized dental pulp extracellular matrix (DP-ECM) in pulpal regeneration. Although the results of the studies suggest that DP-ECM may stimulate pulpal regeneration, the presence of bias and methodological deficiencies limit the ability to draw definitive conclusions. Further research, both clinical and pre-clinical, is needed to provide more conclusive evidence.
Article
Dentistry, Oral Surgery & Medicine
C. Katata, J. Sasaki, A. Li, G. L. Abe, J. E. Nor, M. Hayashi, S. Imazato
Summary: Vascularized DPSC constructs, formed by inducing endothelial differentiation, showed enhanced blood supply and pulp regeneration when transplanted into human pulpless teeth, compared to DPSC constructs without prevascularization. This study demonstrates the potential of vascularized DPSC constructs as a biomaterial for novel dental pulp regeneration.
JOURNAL OF DENTAL RESEARCH
(2021)
Review
Biochemistry & Molecular Biology
Zhuo Xie, Zongshan Shen, Peimeng Zhan, Jiayu Yang, Qiting Huang, Shuheng Huang, Lingling Chen, Zhengmei Lin
Summary: Treatment of pulpal and periapical diseases faces challenges, but significant progress has been made in achieving true pulp tissue regeneration through technologies such as stem cells, biomaterials, and growth factors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Ella Ohlsson, Kerstin M. Galler, Matthias Widbiller
Summary: Efforts in tissue engineering to repair damaged dental pulp tissue have shown positive results, but clinical measures cannot distinguish between true regeneration and repair. Therefore, research models are crucial in understanding treatment outcomes and advancing dental pulp regeneration. This review provides information on various in vitro and in vivo models, discusses the differences between monolayer and three-dimensional cell cultures, presents a new in vivo transplantation model for dental pulp regeneration, and introduces different animal models used for in vivo research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Matthias Widbiller, Andreas Rosendahl, Melanie Woelflick, Moritz Linnebank, Benedikt Welzenbach, Karl-Anton Hiller, Wolfgang Buchalla, Kerstin M. Galler
Summary: In this study, a chairside protocol using endogenous dentin matrix proteins for dental pulp regeneration was designed. The effects of disinfectants on growth factors in the root canal were determined to develop a translational protocol for clinical application. It was found that growth factors can be successfully extracted from root canals and used for tissue engineering.
Review
Dentistry, Oral Surgery & Medicine
Parisa Noohi, Mohammad J. Abdekhodaie, Mohammad H. Nekoofar, Kerstin M. Galler, Paul M. H. Dummer
Summary: This article reviews recent advances in the design and fabrication of scaffolds for the regeneration of pulp-dentine complexes via tissue engineering approaches. Different types of scaffolds and their advantages and limitations are covered. The importance of vascular network establishment for successful pulp-dentine complex regeneration and the corresponding scaffold design strategies are discussed.
INTERNATIONAL ENDODONTIC JOURNAL
(2022)
Review
Engineering, Biomedical
Leanne de Silva, Paulina N. Bernal, A. J. W. Rosenberg, Jos Malda, Riccardo Levato, Debby Gawlitta
Summary: The development of tissue engineering strategies for treatment of large bone defects is crucial, and creating a vascular network is vital for tissue engineered bone constructs.
ACTA BIOMATERIALIA
(2023)
Article
Cell & Tissue Engineering
M. T. Bergamo, Z. Zhang, T. M. Oliveira, J. E. Nor
Summary: The study found that DPSCs contain a unique sub-population of cells with high VEGFR1 expression that are primed to differentiate into vascular endothelial cells, offering the possibility of purifying stem cells with high vasculogenic potential for regeneration of vascularized tissues or in the treatment of ischemic conditions.
EUROPEAN CELLS & MATERIALS
(2021)
Article
Engineering, Biomedical
Sangil Min, David Cleveland, In Kap Ko, Ji Hyun Kim, Hee Jo Yang, Anthony Atala, James J. Yoo
Summary: By incorporating vascular endothelial growth factor (VEGF) into the vascular scaffold, improved angiogenic capability was achieved, promoting vascularization, reducing apoptosis of implanted cells, and forming hybrid renal tubule-like structures.
ACTA BIOMATERIALIA
(2021)
Article
Cell & Tissue Engineering
Jing Li, Youming Zhu, Na Li, Tao Wu, Xianyu Zheng, Boon Chin Heng, Duohong Zou, Jianguang Xu
Summary: By overexpressing ETV2, human dental pulp stem cells (DPSCs) showed significantly enhanced differentiation into endothelial cells, with increased angiogenic potential both in vitro and in vivo. Proteomic analysis revealed upregulation of VEGF receptors, indicating enhanced VEGF signaling. These results suggest that DPSCs could be a promising cell source for tissue engineering applications.
CELL TRANSPLANTATION
(2021)
Article
Dentistry, Oral Surgery & Medicine
Renan Dal-Fabbro, W. Benton Swanson, Leticia C. Capalbo, Hajime Sasaki, Marco C. Bottino
Summary: The current standard for treating irreversibly damaged dental pulp is root canal therapy, but a regenerative approach using tissue engineering and immunomodulatory biomaterials shows promise for better outcomes. This paper provides an overview of the inflammatory process in dental pulp, periapical and periodontal tissue, and discusses recent advances in treating oral diseases using biocompatible materials with immunomodulatory properties. The development of biomaterials that can modulate cells in the dental pulp complex holds significant clinical promise for improving standards of care compared to root canal therapy.
Article
Engineering, Biomedical
Zain Siddiqui, Amanda M. Acevedo-Jake, Alexandra Griffith, Nurten Kadincesme, Kinga Dabek, Dana Hindi, Ka Kyung Kim, Yoshifumi Kobayashi, Emi Shimizu, Vivek Kumar
Summary: Pulp capping and dental pulp regeneration are evolving techniques to regenerate pulp tissue and maintain tooth vitality, with various methods and materials being used in regenerative endodontics.
BIOACTIVE MATERIALS
(2022)
Review
Polymer Science
Vinna K. K. Sugiaman, Jeffrey, Silvia Naliani, Natallia Pranata, Rudy Djuanda, Rosalina Intan Saputri
Summary: The challenge in dentistry is to revitalize dental pulp using tissue engineering technology, which requires a biomaterial scaffold. The scaffold acts as a 3D framework that supports cell growth and provides a suitable environment for cell activation and communication. Selecting a safe, biocompatible scaffold with adequate characteristics is crucial in regenerative endodontics. Natural or synthetic polymer scaffolds have gained attention due to their excellent mechanical properties and good biological characteristics for cell regeneration. This review discusses the latest developments in utilizing these scaffold polymers for dental pulp tissue regeneration.
Review
Dentistry, Oral Surgery & Medicine
Henry F. Duncan
Summary: Therapeutic strategies focused on pulp preservation are important in managing deep caries and exposed pulp in vital teeth. While vital pulp treatments (VPTs) have been used for over a century, recent advancements in regenerative endodontics and biologically based therapies have expanded the indications for VPT to include mature cariously affected teeth and teeth with irreversible pulpitis. However, gaps in scientific knowledge and limited educational dissemination among dentists pose challenges in accurately diagnosing the inflammatory state of the pulp and providing consistent treatment options. This review discusses the current status of VPT, identifies the major problems hindering its clinical acceptance, and offers potential solutions for making VPT a more consistently prescribed evidenced-based treatment in dental practice.
INTERNATIONAL ENDODONTIC JOURNAL
(2022)
Review
Cardiac & Cardiovascular Systems
Mengcheng Shen, Thomas Quertermous, Michael P. Fischbein, Joseph C. Wu
Summary: The developmental origin of vascular smooth muscle cells plays a crucial role in regional susceptibility and resistance to vascular diseases. Patient induced pluripotent stem cell-derived VSMCs are valuable for research and therapeutic applications, but the heterogeneity in developmental origins poses challenges.
CIRCULATION RESEARCH
(2021)
Article
Nanoscience & Nanotechnology
Hossein Montazerian, Avijit Baidya, Reihaneh Haghniaz, Elham Davoodi, Samad Ahadian, Nasim Annabi, Ali Khademhosseini, Paul S. Weiss
Summary: By adding more dopamine moieties to the GelMA prepolymer solution and conducting alkaline dopamine oxidation, the mechanical and adhesive properties of GelMA hydrogels were significantly enhanced. The optimized hydrogels exhibited increased stretchability and toughness, making them suitable for skin-attachable substrates. Further studies on parameters such as UV light, photoinitiator type, and alkaline dopamine oxidation were conducted to tune the cross-linking density for improved adhesive properties.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Engineering, Biomedical
Junmin Lee, Aly Ung, Hanjun Kim, KangJu Lee, Hyun-Jong Cho, Praveen Bandaru, Samad Ahadian, Mehmet R. Dokmeci, Ali Khademhosseini
Summary: Increasing evidence suggests that fusion of cancer cells with different cell types in the tumor microenvironment may contribute to the generation of metastasis-initiating cells. The role of human mesenchymal stem cells (hMSCs) in fusion with cancer cells is still controversial. In this study, a liver-on-a-chip platform was used to investigate the fusion of liver hepatocellular cells (HepG2) with hMSCs and their invasive potential. It was found that hMSCs may play dual roles in HepG2 spheroids, preventing HepG2 growth while also leading to the generation of highly invasive HepG2-hMSC hybrid cells. These hybrid cells expressed markers associated with stemness, proliferation, epithelial to mesenchymal transition, and matrix deposition, and were responsible for collective invasion following HepG2.
Article
Engineering, Biomedical
Anant Bhusal, Elvan Dogan, Hai-Anh Nguyen, Olga Labutina, Daniel Nieto, Ali Khademhosseini, Amir K. Miri
Summary: This study developed a multi-material DLP-based bioprinter for rapid prototyping of hydrogel-based microfluidic chips. The optimized composite hydrogel bioink allows for a wide range of mechanical properties. The biofabrication approach offers a useful tool for integrating micro-tissue models into organs-on-chips and high-throughput drug screening platforms.
Review
Biotechnology & Applied Microbiology
Maryam Tavafoghi, Fatemeh Nasrollahi, Solmaz Karamikamkar, Mahboobeh Mahmoodi, Sara Nadine, Joao F. Mano, Mohammad A. Darabi, Jamileh Jahangiry, Samad Ahadian, Ali Khademhosseini
Summary: Microneedles (MNs) have been developed as minimally invasive tools for diagnostic and therapeutic applications. There is increasing interest in developing smart multifunctional MN devices for body fluid extraction, biosensing, and drug delivery. The main challenge is integrating multiple modules, such as drug carriers and biosensors, in one miniaturized MN device. Researchers have shown the feasibility of creating smart MNs using biomaterials and microscale technologies. Hydrogel-based MN devices show high potential for biomedical applications. The improvement of biomaterials knowledge and biofabrication techniques will allow the development of more effective personalized therapeutics.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Review
Chemistry, Multidisciplinary
Hossein Montazerian, Elham Davoodi, Avijit Baidya, Sevana Baghdasarian, Einollah Sarikhani, Claire Elsa Meyer, Reihaneh Haghniaz, Maryam Badv, Nasim Annabi, Ali Khademhosseini, Paul S. Weiss
Summary: Hemostatic biomaterials have great potential in wound control, particularly for uncontrolled bleeding associated with damaged tissues, traumatic wounds, and surgical incisions. Current research focuses on enhancing the hemostatic properties of bioactive materials through triggering the coagulation cascade, using biocompatible and biodegradable materials for rapid blood coagulation, and developing tough bioadhesives for efficient incision sealing.
Article
Engineering, Biomedical
Edna Johana Bolivar-Monsalve, Carlos Fernando Ceballos-Gonzalez, Carolina Chavez-Madero, Brenda Guadalupe de la Cruz-Rivas, Silvana Velasquez Marin, Shirley Mora-Godinez, Luisa Maria Reyes-Cortes, Ali Khademhosseini, Paul S. Weiss, Mohamadmahdi Samandari, Ali Tamayol, Mario Moises Alvarez, Grissel Trujillo-de Santiago
Summary: This study presents an effective and practical method for the fabrication of pre-vascularized engineered tissues. By co-extruding cell-laden hydrogels and sacrificial materials through printheads containing Kenics static mixing elements, thin hydrogel filaments containing dozens of hollow microchannels as small as a single cell are continuously and one-step fabricated. The hollow channels in the filaments promote cell viability, metabolic activity, proliferation, and cell alignment.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Materials Science, Biomaterials
Marvin Mecwan, Reihaneh Haghniaz, Alireza Hassani Najafabadi, Kalpana Mandal, Vadim Jucaud, Johnson V. John, Ali Khademhosseini
Summary: Researchers have successfully engineered a thermoresponsive shear-thinning hydrogel, composed of a thermoresponsive polymer and hemostatic silicate nanodisks, as an injectable hemostat. This hydrogel demonstrates rapid mechanical recovery and temperature-dependent blood coagulation, effectively preventing blood loss. It shows comparable efficacy to a commercially available hemostat and can be easily removed using a cold saline wash.
BIOMATERIALS SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Gang Ge, Kalpana Mandal, Reihaneh Haghniaz, Mengchen Li, Xiao Xiao, Larry Carlson, Vadim Jucaud, Mehmet Remzi Dokmeci, Ghim Wei Ho, Ali Khademhosseini
Summary: Adhesive materials have been receiving increasing attention for their excellent sealing ability. However, existing adhesives typically have weak adhesion strength and deteriorate in harsh environments. In this study, a novel ionogel with tunable mechanical properties is developed using a deep eutectic solvent as the medium for photopolymerization. The ionogel demonstrates fast gelation and bonding without external pressure, high adhesion in various environments, and wide applications in packaging, marine engineering, medical adhesives, and electronic assembly.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Cell & Tissue Engineering
Pauline Cheng, Ahmad Rashad, Ankit Gangrade, Natan Roberto de Barros, Ali Khademhosseini, Jonathan Tam, Padmini Varadarajan, Devendra K. Agrawal, Finosh G. Thankam
Summary: Myocardial infarction leads to the loss of cardiomyocytes, but stem cell therapy shows promise in restoring cardiac function. This article discusses the application of different stem cell phenotypes and strategies to promote their differentiation into cardiomyocytes.
TISSUE ENGINEERING PART B-REVIEWS
(2023)
Article
Engineering, Biomedical
Hossein Montazerian, Alireza Hassani Najafabadi, Elham Davoodi, Rasoul Seyedmahmoud, Reihaneh Haghniaz, Avijit Baidya, Wei Gao, Nasim Annabi, Ali Khademhosseini, Paul S. Weiss
Summary: Mussel-inspired catechol-functionalization of degradable natural biomaterials is proposed as a potential approach to achieve bioadhesion for sutureless wound closure. This approach involves a simple oxidative polymerization step before conjugation of catechol-carrying molecules to amplify catechol function in bioadhesion. The modified gelatin with poly(l-DOPA) moieties shows improved wound control and enhanced cohesion, surpassing commercial sealants, and also possesses photothermal responsiveness and antibacterial activity, making it an effective biomaterial design strategy for wound closure applications.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Materials Science, Multidisciplinary
Hossein Montazerian, Shameek Mitra, Alireza Hassani Najafabadi, Rasoul Seyedmahmoud, Yuting Zheng, Mehmet Remzi Dokmeci, Nasim Annabi, Ali Khademhosseini, Paul S. Weiss
Summary: Inspired by mussel adhesion, catechol functionalization has been widely used to impart adhesion to biomaterials. However, the bioadhesion capacity of catechol motifs in hydrogels that crosslink through free-radical-based systems is debated. This study reevaluates the bioadhesion efficacy of catechol functionalized gelatin biomolecules in methacryloyl-modified photo-cross-linkable biomaterials, and investigates the effects of catechol content on adhesion strength and hydrogel integrity. The cytotoxic and immunostimulatory effects of catechol groups are also evaluated for clinical applications.
ACS MATERIALS LETTERS
(2023)
Article
Chemistry, Analytical
Safoora Khosravi, Saeid Soltanian, Amir Servati, Ali Khademhosseini, Yangzhi Zhu, Peyman Servati
Summary: Wearable sweat biosensors embedded in textile substrates have gained attention for noninvasive monitoring of health parameters. A flexible electrochemical glucose sensor that can be screen-printed onto a textile substrate is demonstrated, achieving a linear response in the range of 20-1000μM of glucose concentration with high sensitivity and stability. These textile-based biosensors show high selectivity towards glucose and have the potential to impact the next generation of wearable devices.
Review
Automation & Control Systems
Baishali Kanjilal, Yangzhi Zhu, Vaishali Krishnadoss, Janitha M. M. Unagolla, Parnian Saemian, Alessia Caci, Danial Cheraghali, Iman Dehzangi, Ali Khademhosseini, Iman Noshadi
Summary: Ionic liquids (ILs) have unique properties that make them promising candidates for biomedical applications, but their cytocompatibility limitations are enhanced by using bioionic liquids (BILs) derived from biological molecules. BILs can be synthesized and immobilized onto biopolymers, and their functionalization enables the design of responsive actuators and sensors. The cytocompatibility of BIL-functionalized polymers also makes them suitable for power storage and implantable devices. This review focuses on the recent advances of BILs in biomedical applications, specifically their use as functionalization agents for biopolymers and solvents for supermolecular ionic networks.
ADVANCED INTELLIGENT SYSTEMS
(2023)
Article
Chemistry, Multidisciplinary
Bruna Gregatti Carvalho, Aya Nakayama, Hiromi Miwa, Sang Won Han, Lucimara Gaziola de la Torre, Dino Di Carlo, Junmin Lee, Han-Jun Kim, Ali Khademhosseini, Natan Roberto de Barros
Summary: A novel mRNA-releasing matrix based on GelMA microporous annealed particle scaffolds is reported. The sustained release of mRNA complexes achieves indirect intracellular delivery, while direct intracellular delivery is achieved by cell adhesion on the mRNA-releasing scaffolds. This hybrid system demonstrates efficient protein expression, offering potential for mRNA-releasing biomaterials in tissue engineering.
Review
Chemistry, Multidisciplinary
Hossein Montazerian, Elham Davoodi, Avijit Baidya, Maryam Badv, Reihaneh Haghniaz, Arash Dalili, Abbas S. Milani, Mina Hoorfar, Nasim Annabi, Ali Khademhosseini, Paul S. Weiss
Summary: This review provides a biomacromolecular design roadmap for the development of tough adhesive surgical sealants. The intrinsic toughness and elasticity of polymers are achieved through the introduction of strong and dynamic inter- and intramolecular interactions, either through polymer chain design or the use of crosslink regulating additives. Efforts have also been made to promote underwater adhesion through covalent/noncovalent bonds and micro/macro-interlock mechanisms. The measurement and reporting requirements for fair comparisons of different materials and their properties are discussed.
CHEMICAL SOCIETY REVIEWS
(2022)