Article
Biochemical Research Methods
Sunil Kumar Gaire, Yanhua Wang, Hao F. Zhang, Dong Liang, Leslie Ying
Summary: The study aims to accelerate three-dimensional SMLM imaging by leveraging a computational approach to reconstruct high-density 3D images from low-density ones without compromising resolution, achieving superior reconstruction results and reducing the number of acquired frames.
JOURNAL OF BIOMEDICAL OPTICS
(2021)
Article
Chemistry, Analytical
Jinxiu Wei, Siyao Zhang, Jiangnan Yuan, Zhuyuan Wang, Shenfei Zong, Yiping Cui
Summary: Tumor cell exosomes play a vital role in tumor cell proliferation and metastasis, but we still lack a comprehensive understanding of their appearance and biological characteristics due to their nanoscale size and high heterogeneity. We propose an imaging method called Expansion SMLM (ExSMLM) that combines Expansion Microscopy (ExM) and Single Molecule Localization Microscopy (SMLM) to achieve the expansion and super-resolution imaging of tumor cell exosomes.
Article
Optics
Gwiyeong Moon, Taehwang Son, Hajun Yoo, Changhun Lee, Hyunwoong Lee, Seongmin Im, Donghyun Kim
Summary: The interaction between single emitters and plasmonic structures can enhance optical properties but also causes distortion. By studying axially defocused images, the distortion can be explored and the precise positions of emitters can be retrieved, leading to improved localization accuracy.
LIGHT-SCIENCE & APPLICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Wei-Hong Yeo, Yang Zhang, Amy E. Neely, Xiaomin Bao, Cheng Sun, Hao F. Zhang
Summary: Single-molecule localization microscopy (SMLM) allows visualization of cellular nanostructures in vitro with sub-20 nm resolution. However, understanding the structural information from SMLM images has been challenging. To address this, a Monte Carlo (MC) simulation based on experimental imaging parameters was developed to generate synthetic SMLM images. This MC model was used to optimize a clustering algorithm for the separation of fluorescently labeled nuclear pore complex (NPC) proteins and to generate cellular substructures with different angles of labeling.
Article
Optics
Oumeng Zhang, Zijian Guo, Yuanyuan He, Tingting Wu, Michael D. Vahey, Matthew D. Lew
Summary: Single-molecule orientation-localization microscopy is a powerful tool for studying biochemical processes, but the limited photon budget poses challenges for high-dimensional imaging. A raMVR microscope has been developed with isotropic 3D spatial resolution and precision in measuring molecular orientations, enabling detailed imaging of molecular dynamics within biological and chemical systems.
Article
Microbiology
Fabien Rideau, Audrey Villa, Pauline Belzanne, Emeline Verdier, Eric Hosy, Yonathan Arfi
Summary: Mycoplasmas are important models in biology and problematic pathogens in medicine and veterinary fields. Their small size limits the usefulness of traditional fluorescence imaging methods, but recently developed superresolution imaging techniques can break this limit and offer new insights into the organization of these bacteria. This study describes an efficient protocol to perform superresolution microscopy imaging in Mycoplasma, showcasing the potential of these techniques to study subcellular structures at the nanometer scale.
MICROBIOLOGY SPECTRUM
(2022)
Article
Optics
Eleanor Munger, Miguel Sison, Sophie Brasselet
Summary: Single Molecule Orientation and Localization Microscopy (SMOLM) is gaining popularity in the field of localization microscopy due to its ability to monitor orientation as well as spatial information. This study quantifies the bias introduced by incident polarization in SMOLM readout using analytical derivations, and demonstrates this effect experimentally on single molecules attached to a surface with wobbling.
OPTICS COMMUNICATIONS
(2023)
Article
Cell Biology
Takahiro K. Fujiwara, Taka A. Tsunoyama, Shinji Takeuchi, Ziya Kalay, Yosuke Nagai, Thomas Kalkbrenner, Yuri L. Nemoto, Limin H. H. Chen, Akihiro C. E. Shibata, Kokoro Iwasawa, Ken P. Ritchie, Kenichi G. N. Suzuki, Akihiro Kusumi
Summary: The ultrafast camera developed by Fujiwara et al. has significantly improved the time resolution of single-molecule localization microscopy, revealing the dynamic nano-architecture of focal adhesions and leading to the model of compartmentalized focal adhesion protein islands.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Chemistry, Physical
Weiqing Yang, Zhihong Wei, Yan Nie, Yuxi Tian
Summary: Since its first optical detection in 1989, single-molecule spectroscopy has rapidly developed and been widely applied. However, the low photon emission efficiency of majority of matter limits the applications of photoluminescence-based optical methods. Efforts have been made to directly image nonfluorescent matter using different methods based on light-matter absorption or scattering. In this review, we discuss five popular methods, including dark-field microscopy (DFM), surface plasmon resonance microscopy (SPRM), surface enhanced Raman microscopy (SERM), interferometric scattering microscopy (iSCAT), and photothermal microscopy (PTM), for imaging nonfluorescent particles/molecules. We summarize the principles, applications, advantages, and disadvantages of each method and discuss further development and applications.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2022)
Article
Materials Science, Multidisciplinary
Zachary Hallenbeck, Esther A. Wertz
Summary: This work introduces a new technique that combines super-resolution microscopy with fluorescence lifetime imaging microscopy to study the enhancement of single-molecule decay rate by plasmonic nanoparticles. The technique provides high spatial resolution and temporal resolution, allowing for a better understanding of the mechanisms of plasmon-enhanced emission.
ADVANCED OPTICAL MATERIALS
(2022)
Review
Chemistry, Analytical
Hua Liu, Zhongju Ye, Yanan Deng, Jie Yuan, Lin Wei, Lehui Xiao
Summary: This review describes the basic principles of single-molecule localization microscopy (SMLM), factors affecting imaging quality, recent achievements in the development of fluorescent probes for SMLM, and challenges and future advances in SMLM for biological applications.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Jia-Ye Li, De-Yi Zhang, Sheng Mao, Huan Wang
Summary: Few single-molecule experiments have enabled real-time imaging of functional biomacromolecules in native liquid environments. Liquid phase electron microscopy (LP-EM) has shown promise due to its spatial and temporal resolution. Challenges remain in obtaining high-quality data, which may be improved through new liquid cell platforms and machine learning-based data analysis.
CHINESE JOURNAL OF CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Nazar Oleksiievets, Christeena Mathew, Jan Christoph Thiele, Jose Ignacio Gallea, Oleksii Nevskyi, Ingo Gregor, Andre Weber, Roman Tsukanov, Joerg Enderlein
Summary: This study compares the performance of wide-field and confocal FL-SMLM techniques in different spectral regions and provides practical advice.
Article
Multidisciplinary Sciences
Simao Coelho, Jongho Baek, James Walsh, J. Justin Gooding, Katharina Gaus
Summary: Two-photon direct laser writing enables nanometer-accuracy fabrication of three-dimensional structures, providing high-resolution imaging possibilities for optical microscopy.
NATURE COMMUNICATIONS
(2022)
Article
Biochemical Research Methods
Vincent Ebert, Patrick Eiring, Dominic A. Helmerich, Rick Seifert, Markus Sauer, Soren Doose
Summary: Characterizing the spatial fluorophore distribution using convex hull and related properties of localization clusters can be used for diagnostic purposes, as a parameter for cluster selection, or as a tool to determine localization precision.