Journal
NATURE NEUROSCIENCE
Volume 25, Issue 7, Pages 956-+Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41593-022-01095-5
Keywords
-
Categories
Funding
- NMRC [NMRC/MOHIAFCAT2/005/2015, NMRC/OFLCG/002/2018, MH 095:003\016-0002, MH 095:003\016-0001, CIRG19may0052, MOH-CSAINV19nov-0004, PAEDSCAP-COLLABORATION-2021-002]
- Duke-NUS
- SingHealth AMC
- National Research Foundation Singapore under its NMRC Centre Grant Program [NMRC/CG/M003/2017]
Ask authors/readers for more resources
This study revealed the pro-inflammatory immune microenvironment in human epileptic tissue and direct interaction between microglia and T cells. These findings can contribute to the development of new therapeutics.
Epileptogenic triggers are multifactorial and not well understood. Here we aimed to address the hypothesis that inappropriate pro-inflammatory mechanisms contribute to the pathogenesis of refractory epilepsy (non-responsiveness to antiepileptic drugs) in human patients. We used single-cell cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq) to reveal the immunotranscriptome of surgically resected epileptic lesion tissues. Our approach uncovered a pro-inflammatory microenvironment, including extensive activation of microglia and infiltration of other pro-inflammatory immune cells. These findings were supported by ligand-receptor (LR) interactome analysis, which demonstrated potential mechanisms of infiltration and evidence of direct physical interactions between microglia and T cells. Together, these data provide insight into the immune microenvironment in epileptic tissue, which may aid the development of new therapeutics. Single-cell analysis of immune cells from surgically resected human epileptic brain tissues showed heterogeneity and pro-inflammatory signaling in microglia and evidence for direct interaction of microglia with T cells.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available