Journal
NATURE IMMUNOLOGY
Volume 23, Issue 7, Pages 1021-+Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41590-022-01255-6
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Funding
- Ministry of Science and Technology of China [2018YFA0507402]
- National Natural Science Foundation of China [81761128009, 32000667]
- Shanghai Science and Technology Innovation Action [21ZR1470600]
- Youth Innovation Promotion Association of the Chinese Academy of Sciences [2022264]
- Affiliated Hospital of Guangdong Medical University `Clinical Medicine+' CnTech Co-operation Project [CLP2021B001, CLP2021B017]
- Discipline Construction Project of Guangdong Medical University [4SG21231G]
- Project of Zhanjiang City [2018A01025, 2020A01016, 2021A05052]
- Natural Science Foundation of Guangdong Province, China [2021A1515011062, 2022A1515011731]
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Sun and colleagues demonstrate that the secretion of IL-33 after allergen exposure is mediated by stress granule assembly and the generation of a neo-form p40 fragment of gasdermin D. These findings provide insights into the molecular mechanisms of allergen-induced airway inflammation and offer potential targets for the treatment of allergic respiratory diseases.
Sun and colleagues describe that the secretion of interleukin-33 is dependent on a p40 N-terminal fragment of gasdermin D, whose generation is independent of inflammatory caspase-1 and caspase-11. Interleukin-33 (IL-33), an epithelial cell-derived cytokine that responds rapidly to environmental insult, has a critical role in initiating airway inflammatory diseases. However, the molecular mechanism underlying IL-33 secretion following allergen exposure is not clear. Here, we found that two cell events were fundamental for IL-33 secretion after exposure to allergens. First, stress granule assembly activated by allergens licensed the nuclear-cytoplasmic transport of IL-33, but not the secretion of IL-33. Second, a neo-form murine amino-terminal p40 fragment gasdermin D (Gsdmd), whose generation was independent of inflammatory caspase-1 and caspase-11, dominated cytosolic secretion of IL-33 by forming pores in the cell membrane. Either the blockade of stress granule assembly or the abolishment of p40 production through amino acid mutation of residues 309-313 (ELRQQ) could efficiently prevent the release of IL-33 in murine epithelial cells. Our findings indicated that targeting stress granule disassembly and Gsdmd fragmentation could reduce IL-33-dependent allergic airway inflammation.
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