Review
Engineering, Biomedical
Prerak Gupta, Biman B. Mandal
Summary: Vascular tissue engineering is focusing on finding biologically active substitutes with in situ growth potential, with silk biomaterials showing remarkable potential due to their favorable biological and mechanical properties.
ACTA BIOMATERIALIA
(2021)
Review
Biochemistry & Molecular Biology
Minghui Li, Jiaqian You, Qiuyue Qin, Manxuan Liu, Yixin Yang, Kewen Jia, Yidi Zhang, Yanmin Zhou
Summary: Bone tissue engineering (BTE) utilizes silk fibroin (SF) to regulate bone regeneration and stimulate bone formation. SF is a promising material due to its tunable mechanical properties, biodegradability, and biocompatibility. This review introduces the structure and characteristics of SF, summarizes its immunological mechanism for osteogenesis, and emphasizes the various forms of SF biomaterials and their development prospects in BTE. Biomaterials based on SF have great potential in bone tissue engineering, and this review guides future design and research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Chemistry, Physical
Federica Paladini, Mauro Pollini
Summary: Bone tissue engineering is a multidisciplinary research field that aims to create biological substitutes for bone regeneration. The selection of appropriate biomaterials and structures remains a challenge, and silk fibroin is considered a valuable option due to its unique biological properties and processability.
Article
Chemistry, Applied
Weiguang Zhao, Shiyi Cao, Haoxin Cai, Yang Wu, Qi Pan, Heng Lin, Jun Fang, Yinyan He, Hongbing Deng, Zhihong Liu
Summary: This study addresses the challenge of reconstructing multilayered and functional smooth muscle tissues by incorporating cellulose acetate electrospun nanofibers into chitosan/silk fibroin cryogel scaffolds. The composite scaffolds showed improved mechanical properties and enhanced proliferation and infiltration of smooth muscle cells. The findings indicate the potential of these scaffolds for smooth muscle tissue engineering.
CARBOHYDRATE POLYMERS
(2022)
Review
Biochemistry & Molecular Biology
Weizhen Sun, David Alexander Gregory, Mhd Anas Tomeh, Xiubo Zhao
Summary: Tissue engineering involves combining cells, scaffold materials, and growth factors to regenerate or replace damaged tissues or organs. Silk fibroin has attracted attention for its outstanding properties and ability to be reconstructed into various material formats for tissue engineering applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Guangfei Li, Shan Sun
Summary: Silk fibroin scaffolds are widely used materials in tissue engineering, with excellent properties and diverse forms that can be achieved through various fabrication techniques. These scaffolds play a significant role in various tissue engineering applications.
Article
Polymer Science
Alessio Bucciarelli, Mauro Petretta, Brunella Grigolo, Laura Gambari, Alessandra Maria Bossi, Francesco Grassi, Devid Maniglio
Summary: In this study, a crosslinked methacrylated silk (Sil-MA) grid structure was developed, which can recover its shape under UV light and has biocompatibility and osteogenic support. Sil-MA was chemically and physically characterized, and tested for its biocompatibility according to ISO protocols. Digital Light Processing and pneumatic extrusion techniques were used to manufacture the Sil-MA grid structure, demonstrating its shape memory property and potential application in bone regeneration.
Article
Biochemistry & Molecular Biology
Giriprasath Ramanathan, Grace Felciya Sekar Jeyakumar, Uma Tiruchirapalli Sivagnanam, Pedro Fardim
Summary: In this study, a porous hybrid matrix (CEL-COL-SF) was fabricated using microcrystalline cellulose (CEL), collagen (COL), and silk fibroin (SF). The hybrid matrix exhibited interconnected porous architecture, excellent oxygen permeability, and promoted cell adhesion, proliferation, migration, and minerals deposition. It also showed excellent biocompatibility and cell proliferation when tested with fibroblast and osteoblast-like cells. The matrix demonstrated great potential in bone tissue engineering, as it exhibited the sequential events related to bone regeneration and mimicked the atomic ratio of calcium to phosphate in bone.
PROCESS BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Monika Rajput, Pritiranjan Mondal, Parul Yadav, Kaushik Chatterjee
Summary: Three-dimensional bioprinting based on digital light processing (DLP) provides opportunities for mimicking human tissues. However, the limited availability of biocompatible and biodegradable bioinks hinders progress in this field. This study presents a silk fibroin-derived bioink for DLP bioprinting, which exhibits bone-like viscoelastic behavior and favorable degradation. The bioink supports cell proliferation and osteogenesis. This work highlights the importance of silk fibroin-derived bioinks in tissue engineering.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Xiaoxuan Wang, Yan Peng, Yang Wu, Shiyi Cao, Hongbing Deng, Zhengguo Cao
Summary: In this study, bilayer-deposited multifunctional nanofiber mats with osteogenic and antibacterial sides were successfully fabricated. These mats showed good biocompatibility, hydrophilicity, mechanical properties, and antibacterial potency. Gene delivery of Runx2 enhanced osteogenesis and bone tissue generation in vivo. These results suggest the excellent prospects of using PCSP mats for bone regeneration.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Natasha Bokhari, Asif Ali, Abida Yasmeen, Hamad Khalid, Sher Zaman Safi, Faiza Sharif
Summary: Soft tissue defects can be resolved with coated silk meshes, which have antimicrobial and wound healing properties. The coating alters the pore size and fiber diameter of the mesh, while also improving its mechanical strength. Among the variants tested, MHRJ showed the best cell viability and wound healing effects. In vivo experiments with rat hernia model confirmed the non-toxic nature and wound healing characteristics of the modified mesh. This study suggests that MHRJ could be a valuable biomedical implant.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Engineering, Biomedical
Joseph Christakiran Moses, Souradeep Dey, Ashutosh Bandyopadhyay, Manoj Agarwala, Biman B. Mandal
Summary: The development of a sandwich-type biofabricated diaphyseal cross-sectional unit, encompassing an outer mechanically robust bioprinted cortical bone shell and an inner engineered bone marrow, holds promise as a potential orthobiologic substitute for the treatment of atrophic nonunion fractures.
ADVANCED HEALTHCARE MATERIALS
(2022)
Review
Medicine, Research & Experimental
Augusto Zuluaga-Velez, Adrian Quintero-Martinez, Lina M. Orozco, Juan C. Sepulveda-Arias
Summary: Silk fibroin nanocomposites have great potential in tissue engineering, with the ability to enhance stability and mechanical performance by incorporating inorganic phases and influencing specific biological responses. However, challenges still exist in their application to other tissues, calling for further research on the interaction between inorganic and organic phases and the associated biological responses.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Engineering, Biomedical
Xin Liu, Yuxiang Sun, Bo Chen, Yan Li, Peng Zhu, Peng Wang, Sen Yan, Yao Li, Fang Yang, Ning Gu
Summary: A novel magnetic SF scaffold (MSFCs) was prepared by an improved infiltration method, exhibiting better crystallinity, magnetocaloric properties, and mechanical strength compared to SF scaffold (SFC). The delayed degradation of MSFCs was attributed to the formation of hydrogen bonds and complexing to tyrosine to inhibit hydrolase by internal iron atoms. Doping MNPs showed a beneficial effect on beta-crystallization of protein and did not affect the function of macrophages.
BIOACTIVE MATERIALS
(2022)
Article
Biophysics
Yun-Fei Shao, Xiangcheng Qing, Yizhong Peng, Hui Wang, Zengwu Shao, Ke-Qin Zhang
Summary: By comparing the effects of microwave-assisted technology and biomineralization methods on the HA coating, this study found that microwave-assisted technology can enhance the deposition efficiency and compressive strength of HA in 3D scaffolds, while also promoting cell adhesion and osteogenic differentiation.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)