Design of two-dimensional molybdenum trioxide-immobilized magnetic graphite nitride nanocomposites with multiple affinity sites for phosphopeptide enrichment

Here, two-dimensional magnetic graphite nitride nanosheets were synthesized, over which molybdenum trioxide was distributed evenly. The magnetic nanocomposite served as an enrichment platform for phosphopeptides based on metal oxide affinity chromatography and anion-exchange chromatography. Owing to the abundant affinity sites, positive electricity, and large surface area, the nanocomposite had high selectivity (alpha-casein/beta-casein/bovine serum albumin, 1:1:5000), sensitivity (0.1 fmol), quantitative recovery (91.3%), and good recyclability (10 cycles). The nanocomposite also showed good enrichment performance for phosphopeptides from non-fat milk, human serum, saliva, and A549 cell lysate. The results demonstrated the value of this material in phosphorylated proteomics. (C) 2022 Elsevier B.V. All rights reserved.

Automated summary

In this study, two-dimensional magnetic graphite nitride nanosheets were synthesized and distributed with molybdenum trioxide. The magnetic nanocomposite exhibited high selectivity, sensitivity, quantitative recovery, and recyclability for phosphopeptide enrichment. It also showed excellent performance in enriching phosphopeptides from various samples, highlighting its significance in phosphorylated proteomics.