Article
Biochemistry & Molecular Biology
Caitlin E. Miller, Fangfang Xu, Yanming Zhao, Wei Luo, Weixiong Zhong, Kristy Meyer, Rani Jayswal, Heidi L. Weiss, William H. St Clair, Daret K. St Clair, Luksana Chaiswing
Summary: This study reveals a novel adaptive response to radiation treatment that promotes cancer recurrence. It shows that hydrogen peroxide (H2O2) can reprogram mitochondrial homeostasis in prostate cancer cells, allowing them to survive and regrow after radiation treatment. The study also demonstrates that radiation induces the release of extracellular vesicles (EVs) carrying mitochondrial antioxidant proteins, and H2O2 enhances the production of these EVs. Furthermore, functional mitochondria enhance cancer cell survival after radiation treatment.
Article
Food Science & Technology
Yang He, Hammed Ayansola, Qihang Hou, Chaoyong Liao, Jiaqi Lei, Yujiao Lai, Qiuyu Jiang, Hori Masatoshi, Bingkun Zhang
Summary: Genistein alleviates colitis caused by Salmonella infection by regulating gut bacteria and intestinal stem cell development, inhibiting goblet cell loss.
MOLECULAR NUTRITION & FOOD RESEARCH
(2021)
Article
Chemistry, Analytical
Jiaxin Hong, Xiaogang Guan, Yao Chen, Xiaodong Tan, Shiya Zhang, Guoqiang Feng
Summary: Mitophagy is a crucial cellular process that plays vital roles in regulating cellular metabolism and mitochondrial quality control. Mitochondrial viscosity, closely associated with mitochondrial status, is a key microenvironmental index. To monitor mitophagy and mitochondrial viscosity changes, a probe called Mito-3 was developed. Mito-3, along with other probes, showed sensitivity to viscosity changes and fluorescence enhancement. These probes were successfully used to locate and visualize mitochondria, as well as monitor mitochondrial viscosity changes in cells. Additionally, Mito-3 was applied to visualize the mitophagy process induced by starvation and revealed an increase in mitochondrial viscosity during mitophagy.
ANALYTICAL CHEMISTRY
(2023)
Article
Neurosciences
Clara Voelz, Pardes Habib, Stefan Koeberlein, Cordian Beyer, Alexander Slowik
Summary: This study investigated the impact of oxygen-glucose deprivation (OGD) and hydrogen peroxide (H2O2) on the expression of miRNA-regulating proteins in human microglial cells, revealing induction of DICER, DROSHA, and AGO2 under hypoxia-like conditions. Additionally, XPO5 and TARBP2 were found to be upregulated in response to H2O2, while DGCR8 responded only to OGD. These findings indicate a potential increase in miRNA synthesis rate under stress conditions.
MOLECULAR NEUROBIOLOGY
(2021)
Article
Environmental Sciences
Faroza Nazir, Qazi Fariduddin, Anjuman Hussain, Tanveer Alam Khan
Summary: This study investigated the effects of brassinosteroids and hydrogen peroxide on tomato plants under copper stress. The results showed that EBL and H2O2 applied through distinct modes could enhance the resilience of tomato plants to copper stress.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Plant Sciences
Rosario Pantaleno, Denise Scuffi, Alex Costa, Elina Welchen, Roberta Torregrossa, Matthew Whiteman, Carlos Garcia-Mata
Summary: Hydrogen sulfide (H2S) is a gaseous signaling molecule that plays a role in physiological processes in plants, including regulating stomatal pore width. In this study, it was found that a mitochondrial-targeted H2S donor called AP39 can induce stomatal closure in a dose-dependent manner. The activity of mitochondrial cytochrome c (CYTc) and/or complex IV was shown to be necessary for AP39-dependent stomatal closure. AP39 also affected the mitochondrial inner potential, cytosolic ATP, hydrogen peroxide levels, and the oxidation of the glutathione pool in guard cells. These findings highlight the interplay between mitochondrial H2S, mitochondrial activity, and stomatal closure.
Review
Biochemistry & Molecular Biology
Sergio Rius-Perez, Salvador Perez, Michel B. Toledano, Juan Sastre
Summary: Redox signaling through mtROS plays a crucial role in multiple mechanisms of regulated cell death and contributes decisively to the development of inflammatory disorders.
ANTIOXIDANTS & REDOX SIGNALING
(2023)
Article
Chemistry, Multidisciplinary
Xin Wang, Qi Ding, Ying Tian, Wei Wu, Feida Che, Ping Li, Wen Zhang, Wei Zhang, Bo Tang
Summary: This study developed two fluorescent probes to monitor H2O2 in mitochondria and lysosomes. The findings from fluorescence imaging demonstrated that mitochondrial H2O2 is involved in reduced GCase activity in lysosomes in depressed mice, providing a valuable tool for understanding the pathogenesis of depression.
CHEMICAL COMMUNICATIONS
(2022)
Editorial Material
Energy & Fuels
Yuanxing Fang, Xinchen Wang
Summary: Photocatalytic hydrogen peroxide synthesis is a green method to produce this commonly used chemical and potential energy carrier, but its performance is often inadequate. A porphyrin-based photocatalyst has been demonstrated to generate hydrogen peroxide efficiently through an unconventional mechanism involving both photoexcited electrons and holes.
Article
Chemistry, Multidisciplinary
Shaoda Huang, Bingyan Zhang, Huimin Sun, Hongyin Hu, Jinyan Wang, Fang Duan, Han Zhu, Mingliang Du, Shuanglong Lu
Summary: We developed a series of single atom catalysts anchored on bipyridine-rich COFs. The optimal Py-Bpy-COF-Zn shows the highest selectivity of 99.1% and excellent stability in H2O2 production via oxygen reduction, attributed to its high *OOH dissociation barrier. As a proof of concept, it acts as a cathodic catalyst in a homemade Zn-air battery and enables efficient wastewater treatment.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Meng Li, Xiaoming Luo, Shan Lei, Yurong Liu, Huishan Guo, Yu Zhang, Yuantao Pan, Kaiwei Chen, Jing Lin, Peng Huang
Summary: A glycopolymer containing a caged H2S and H2O2 dual-donor is developed to wrap glucose oxidase, which can deplete tumorigenic energy sources and block glycolysis. This strategy induces defects in mitochondrial function and shows good anticancer performance.
ADVANCED MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Ruijun Pan, Xinmiao Lu, Junhui Ju, Qinghua Guan, Yue Su, Chunting Li, Peiyong Li
Summary: A ratiometric fluorescence nanoprobe was developed for CTC enumeration and H2O2 evaluation, effectively identifying CTCs and assessing their viability, providing significant guidance for clinical treatments. The combination of viability CTC count and intracellular H2O2 content was related to the tumor TNM stage, showing promise for clinical applications.
BIOORGANIC & MEDICINAL CHEMISTRY
(2021)
Article
Immunology
Zheng Gong, Qing Li, Jiayuan Shi, Guangwen Ren
Summary: Intracellular cytokine staining (ICS) is commonly used to determine the activation status of immune cells, but the presence of neutrophils can lead to overactivation and death of T cells, potentially misleading immunological research.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Gastroenterology & Hepatology
Lei Sun, Arabella Wan, Zhuolong Zhou, Dongshi Chen, Heng Liang, Chuwei Liu, Shijia Yan, Yi Niu, Ziyou Lin, Siyue Zhan, Shanfeng Wang, Xianzhang Bu, Weiling He, Xiongbin Lu, Anlong Xu, Guohui Wan
Summary: The study identified an RNA-binding protein RALY as a critical regulator in colorectal cancer metabolism, promoting the post-transcriptional processing of miRNAs that downregulate metabolism-associated genes and reprogram mitochondrial metabolism in cancer cells. Increased levels of RALY were associated with poor prognosis in CRC patients expressing low levels of mitochondrion-associated genes, and this effect was facilitated by N6-methyladenosine switch under reactive oxygen species stress. Inhibition of m6A methylation abolished RALY recognition of pri-miRNAs, leading to inhibition of colorectal tumor growth and progression both in vivo and in organoid models.
Article
Chemistry, Multidisciplinary
Yan Zhao, Xinquan Yu, Weiheng Kong, Rong-Mei Kong, Ensheng Zhang, Lian Xia, Jing Zhang, Fengli Qu, Weihong Tan
Summary: Promoting tumor calcification by inducing mitochondrial metabolic disorder and destroying calcium equilibrium has a potential inhibitory effect on tumor proliferation, which is considered to be a predictor of good prognosis.
Article
Food Science & Technology
Margalida Torrens-Mas, Marina Alorda-Clara, Maria Martinez-Vigara, Pilar Roca, Jorge Sastre-Serra, Jordi Oliver, Daniel Gabriel Pons
Summary: The study found that low doses of XN treatment did not significantly affect cell viability, but led to a general decrease in pro-inflammatory, antioxidant and mitochondrial biogenesis gene expression. Additionally, glucose and oxidative metabolism enzymes were also reduced.
INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION
(2022)
Review
Oncology
Marina Alorda-Clara, Margalida Torrens-Mas, Pere Miquel Morla-Barcelo, Toni Martinez-Bernabe, Jorge Sastre-Serra, Pilar Roca, Daniel Gabriel Pons, Jordi Oliver, Jose Reyes
Summary: Colorectal cancer (CRC) is a highly diagnosed cancer with significant mortality rates, and early detection is crucial for improving patient survival. Current CRC screening programs involve stool tests and colonoscopies, but the use of new biomarkers could enhance screening effectiveness. Omics technologies offer promise in detecting novel biomarkers for CRC across different sample types.
Article
Gastroenterology & Hepatology
Carme Loras, Xavier Andujar, Joan B. Gornals, Vicente Sanchiz, Enric Brullet, Beatriz Sicilia, Maria Dolores Martin-Arranz, Antonio Naranjo, Jesus Barrio, Carmen Duenas, Jose Ramon Foruny, David Busquets, David Monfort, Juan Ramon Pineda, Ferran Gonzalez-Huix, Francisco Perez-Roldan, Vicente Pons, Begona Gonzalez, Jose Reyes Moreno, Empar Sainz, Jordi Guardiola, Marta Maia Bosca-Watts, Fernando Fernandez-Banares, Vicenc Mayor, Maria Esteve
Summary: This study compares the efficacy and safety of endoscopic balloon dilation (EBD) and fully covered self-expandable metal stents (FCSEMS) in the treatment of Crohn's disease strictures, and compares the mean cost of both treatments. The results show that EBD is more effective than FCSEMS for Crohn's disease strictures and both treatments have a good safety profile.
LANCET GASTROENTEROLOGY & HEPATOLOGY
(2022)
Article
Biology
Reyniel Hernandez-Lopez, Margalida Torrens-Mas, Daniel G. Pons, Maria M. Company, Esther Falco, Teresa Fernandez, Javier M. Ibarra de la Rosa, Pilar Roca, Jordi Oliver, Jorge Sastre-Serra
Summary: Metastasis is a crucial factor in the mortality of colorectal cancer (CRC) patients. This study explores the changes in mitochondrial function associated with the metastatic stage of CRC and investigates whether they could be utilized as therapeutic targets. The findings indicate notable differences in mitochondrial functionality between tumor tissue and normal adjacent tissue in metastatic CRC patients. These alterations contribute to the promotion and maintenance of the metastatic phenotype, highlighting the impairment of mitochondrial function in tumor tissue during the metastatic stage.
Article
Oncology
Marina Alorda-Clara, Margalida Torrens-Mas, Reyniel Hernandez-Lopez, Javier M. Ibarra de la Rosa, Esther Falco, Teresa Fernandez, Maria Margarita Company, Jorge Sastre-Serra, Jordi Oliver, Daniel Gabriel Pons, Pilar Roca
Summary: Non-tumor adjacent tissue has an important role in the development and inflammatory process of colorectal cancer. This study analyzed the expression of inflammatory, epithelial-mesenchymal transition (EMT), and metastasis-related proteins in tumor and non-tumor adjacent tissues from colorectal cancer patients. The results showed that tumor tissue had increased levels of metastasis and EMT-related proteins compared to non-tumor adjacent tissue, particularly in stage II. Tumor tissue from stage II also had increased levels of inflammatory-related proteins, which was also observed in non-tumor adjacent tissue from stage II. Additionally, higher expression levels of Vimentin and VEGF-B were associated with lower relapse-free survival in stage II patients. These findings suggest that these proteins could be potential biomarkers for early diagnosis of colorectal cancer.
Article
Biochemistry & Molecular Biology
Toni Martinez-Bernabe, Jordi Oliver, Jorge Sastre-Serra, Daniel Gabriel Pons
Summary: This study analyzed the effect of oxaliplatin on tumor spheres formation efficiency, cell viability, cancer stem cells, and inflammation in primary- and metastatic-derived colorectal tumor spheres. The results showed that primary-derived spheres responded to oxaliplatin by modulating cancer stem cells and stemness properties, while metastatic-derived spheres exhibited an inflammatory response. In addition, the expression of inflammatory markers after oxaliplatin treatment correlated with poor prognosis and a metastatic phenotype. These findings highlight the importance of drug testing and personalized medicine in early-stage colorectal cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Marina Alorda-Clara, Jose Reyes, Marita Grimanesa Trelles-Guzman, Monica Florido, Pilar Roca, Daniel Gabriel Pons, Jordi Oliver
Summary: Colorectal cancer is the third most common cancer worldwide, and the lack of early and specific biomarkers leads to late-stage detection. Tumors release extracellular vesicles (EVs) that have various functions and can be used as a source of biomarkers. Bowel lavage fluid (BLF) obtained during colonoscopy is an underutilized sample that represents EVs from tumor cells and has the potential to be a useful research tool. In this study, EVs from BLF were isolated and characterized, confirming their suitability for CRC study and potential as biomarkers for diagnosis and monitoring.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Ecology
Facundo Luna, Jorge Sastre-Serra, Jordi Oliver, C. Daniel Antenucci
Summary: In mammals, the integrated requirements of all activities must conform to a sustained rate of metabolism for the pup's development and adult life. Partitioning available energy according to short-term priorities allows animals to optimize long-term reproductive success. In this study, the development of thermogenic capacity in altricial rodent pups, specifically Ctenomys talarum, was evaluated. The results showed that non-shivering thermogenesis was absent in the pups until day 60, despite the presence of molecular markers for thermogenesis in brown adipose tissue deposits. However, the total thermogenic capacity became fixed at an early age, indicating an improvement in shivering thermogenesis efficiency after day 10.
FRONTIERS IN ECOLOGY AND EVOLUTION
(2023)
Article
Biochemistry & Molecular Biology
Xavier Capo, Aina Maria Galmes-Panades, Cayetano Navas-Enamorado, Ana Ortega-Moral, Silvia Marin, Marta Cascante, Andres Sanchez-Polo, Luis Masmiquel, Margalida Torrens-Mas, Marta Gonzalez-Freire
Summary: This study investigated the relationship between circulating serum NFL, muscle function, and aging. The results showed that NFL levels increased with age, particularly in men. There were negative associations between NFL levels and measures of muscle function, indicating a decline in muscle performance with increasing NFL. Additionally, NFL levels were correlated with markers of body composition, such as fat mass and muscle mass, predominantly in men. Metabolomic analysis revealed significant associations between NFL levels and specific metabolites, with gender-dependent relationships observed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)