Journal
JOURNAL OF COLLOID AND INTERFACE SCIENCE
Volume 462, Issue -, Pages 19-28Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jcis.2015.09.047
Keywords
Localized Surface Plasmon Resonance; Poly(pentafluorostyrene); Reversible addition-fragmentation chain transfer; Glycocluster effect; Biosensor chips
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Funding
- University of New South Wales (UNSW) Faculty of Engineering Early Career Researcher Faculty Research Grant (ECR/FRG)
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Poly(methyl methacrylate) polymer based Localized Surface Plasmon Resonance biosensor chips were successfully fabricated using glycopolymer brushes carrying glucose moieties for the detection of concanavalin A. Poly(pentafluorostyrene), with pre-determined polymer chain lengths, were synthesized via a reversible addition-fragmentation chain transfer polymerization technique. The synthesized poly (pentafluorostyrene), was subsequently converted into glycopolymers via a para-fluoro-thiol click reaction and grafted onto the surface of sensor chips. The glycocluster effect induced by pendent carbohydrate moieties enabled a stronger affinity for concanavalin A binding, which resulted in a dramatic expansion of the sensors' response range. It was discovered that the longer polymer brushes did not guarantee additional enhancements for the sensor chips. Instead, they could lead to higher detection limits. In this study, the limit of detection for the sensor chips was discovered to be 1.3 nmol L-1 with a saturated response at 1054.2 nmol L-1. In addition to the superior performance, the capabilities of the reported sensor chips can be easily manipulated to detect a diverse range of analytes by clicking various sensing elements onto the polymer brushes. (C) 2015 Elsevier Inc. All rights reserved.
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