Article
Veterinary Sciences
Michihito Tagawa, Minori Aoki
Summary: This study evaluated the utility of circulating cfDNA in canine patients with oral malignant melanoma (OMM) in assessing therapeutic response and clinical outcomes. The results suggest that measurements of serum cfDNA and DII using LINE-1 might be valuable new biomarkers for monitoring OMM progression in dogs.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Review
Medicine, General & Internal
Ugur Gezer, Abel J. Bronkhorst, Stefan Holdenrieder
Summary: Liquid biopsy is a method of diagnosing pathological conditions by testing biomarkers in body fluids. Cell-free DNA (cfDNA) has significant potential as a biomarker in oncology, prenatal testing, and transplant monitoring. The clinical potential of repeat DNA sequences in cfDNA as an underappreciated marker in liquid biopsy for cancer is highlighted.
Review
Oncology
Fumie Igari, Hisashi Tanaka, Armando E. Giuliano
Summary: Liquid biopsy is a rapidly developing field in oncology that detects cancer through DNA, RNA, and protein analysis in body fluids. It provides real-time reporting of tumor burden and is applicable for screening tumor types without standard screening approaches. Genetic mutation and DNA methylation profiling are the main methods for liquid biopsy.
CRITICAL REVIEWS IN ONCOLOGY HEMATOLOGY
(2022)
Article
Oncology
Lars Sivars, Kristina Hellman, Ylva Crona Guterstam, Stefan Holzhauser, Magnus Nordenskjold, Henrik Falconer, Kolbrun Palsdottir, Emma Tham
Summary: This study aimed to analyze the detectability of cell-free human papillomavirus DNA (ctHPV DNA) in patients with cervical cancer or pre-malignant lesions and its correlation with patient and tumor characteristics and outcome. The results showed a high proportion of ctHPV DNA detection in patients with locally advanced cervical cancer, and its levels were correlated with disease staging. Furthermore, the presence of ctHPV DNA was associated with worse progression-free survival in patients.
GYNECOLOGIC ONCOLOGY
(2022)
Article
Multidisciplinary Sciences
Julie Earl, Silvia Calabuig-Farinas, Maria Eugenia Sarasquete, Laura Muinelo Romay, Sara Lopez-Tarruella, Beatriz Bellosillo Paricio, Marta Rodriguez, Karmele Valencia Leoz, Marta Duenas Porto, Noelia Tarazona, Javier Hernandez Losa, Rodrigo Almeida Toledo
Summary: Liquid biopsy is a diagnostic method based on molecules in blood and other bodily fluids, providing a minimally invasive alternative to solid biopsy while allowing real-time monitoring of tumors. Its applications include early cancer detection, disease progression follow-up, minimal residual disease assessment, and identification of molecular progression and resistance mechanisms. Proper preanalytical procedures such as sample collection, quality, and storage are crucial for reliable analysis.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Oncology
Maria Lamminaho, Jouni Kujala, Hanna Peltonen, Maria Tengstrom, Veli-Matti Kosma, Arto Mannermaa
Summary: The study found that high cfDI is an independent prognostic factor for poor overall survival and breast cancer-specific survival in breast cancer cases, and its inclusion in logistic regression models improves predictive performance, suggesting its prognostic potential.
Article
Biology
Ilana Fox-Fisher, Sheina Piyanzin, Bracha Lea Ochana, Agnes Klochendler, Judith Magenheim, Ayelet Peretz, Netanel Loyfer, Joshua Moss, Daniel Cohen, Yaron Drori, Nehemya Friedman, Michal Mandelboim, Marc E. Rothenberg, Julie M. Caldwell, Mark Rochman, Arash Jamshidi, Gordon Cann, David Lavi, Tommy Kaplan, Benjamin Glaser, Ruth Shemer, Yuval Dor
Summary: The immune cell type-specific methylation patterns in circulating cell-free DNA (cfDNA) can be used for studying human immune cell dynamics and provide a novel biomarker for monitoring immune responses to physiological and pathological processes.
Review
Oncology
Lars Sivars, Kolbrun Palsdottir, Ylva Crona Guterstam, Henrik Falconer, Kristina Hellman, Emma Tham
Summary: In this review, we provide an overview of ctDNA and ctHPV DNA research in cervical cancer. Detection of ctHPV DNA in plasma from patients with cervical cancer is feasible and may serve as a promising prognostic biomarker. However, further studies are needed to determine the presence of ctHPV DNA in pre-malignant lesions.
INTERNATIONAL JOURNAL OF CANCER
(2023)
Article
Oncology
Arash Jamshidi, Minetta C. Liu, Eric A. Klein, Oliver Venin, Earl Hubbell, John F. Beausang, Samuel Gross, Collin Melton, Alexander P. Fields, Qinwen Liu, Nan Zhang, Eric T. Fung, Kathryn N. Kurtzman, Hamed Amini, Craig Betts, Daniel Civello, Peter Freese, Robert Calef, Konstantin Davydov, Saniya Fayzullina, Chenlu Hou, Roger Jiang, Byoungsok Jung, Susan Tang, Vasiliki Demas, Joshua Newman, Onur Sakarya, Eric Scott, Archana Shenoy, Seyedmehdi Shojaee, Kristan K. Steffen, Virgil Nicula, Tom C. Chien, Siddhartha Bagaria, Nathan Hunkapiller, Mohini Desai, Zhao Dong, Donald A. Richards, Timothy J. Yeatman, Allen L. Cohn, David D. Thiel, Donald A. Berry, Mohan K. Tummala, Kristi MCIntyre, Mikkael A. Sekeres, Alan Bryce, Alexander M. Aravanis, Michael V. Seiden, Charles Swanton
Summary: In this study, we evaluated several approaches for a circulating cell-free DNA (cfDNA)-based multi-cancer early detection (MCED) test and identified the most promising method, which is the use of whole-genome methylation feature. cTAF is an important predictor of classifier performance and more closely reflects tumor biology.
Article
Biology
Brian Silver, Kevin Gerrish, Erik Tokar
Summary: Cell-free DNA (cfDNA) has the potential to be a noninvasive diagnostic tool for early disease detection. This study investigates cfDNA markers produced by cardiac organoids, showing that cfDNA can be recovered from cardiac organoids and that the release is highest during early differentiation. The study also identifies changes in specific genomic regions and cfDNA markers associated with cardiotoxic drug treatment.
Article
Oncology
Shuxin Zhang, Jun Zhang, Xinlei Hu, Senlin Yin, Yunbo Yuan, Lin Xia, Feng Cao, Xiaoqin Yan, Ziyue Yan, Qing Mao, Dan Xie, Yanhui Liu
Summary: A liquid biopsy method based on sequencing of plasma cfDNA fragments carrying 5hmC has been developed, which can detect brain gliomas and predict the aggressiveness of the tumors.
INTERNATIONAL JOURNAL OF CANCER
(2023)
Article
Medicine, Research & Experimental
Florent Mouliere, Christopher G. Smith, Katrin Heider, Jing Su, Ymke van der Pol, Mareike Thompson, James Morris, Jonathan C. M. Wan, Dineika Chandrananda, James Hadfield, Marta Grzelak, Irena Hudecova, Dominique-Laurent Couturier, Wendy Cooper, Hui Zhao, Davina Gale, Matthew Eldridge, Colin Watts, Kevin Brindle, Nitzan Rosenfeld, Richard Mair
Summary: The study focused on detecting glioma-derived cell-free DNA (cfDNA) in body fluids and found that tumor-derived DNA could be identified in the majority of CSF, plasma, and urine samples with different tumor fractions. They also discovered a significant difference in cfDNA fragment sizes between glioma patients and individuals with non-malignant brain disorders or healthy individuals, which could potentially aid in non-invasive cancer detection using machine learning models.
EMBO MOLECULAR MEDICINE
(2021)
Article
Multidisciplinary Sciences
Gregory E. Alexander, Wendy Lin, Fabian E. Ortega, Madhuvanthi Ramaiah, Byoungsok Jung, Lijuan Ji, Ekaterina Revenkova, Payal Shah, Christian Croisetiere, Jennifer R. Berman, Lane Eubank, Gunjan Naik, Jacqueline Brooks, Andrea Mich, Seyedmehdi Shojaee, Neda Ronaghi, Hemanshi Chawla, Xinyi Hou, Qinwen Liu, Christopher-James A. V. Yakym, Patriss Wais Moradi, Meredith Halks-Miller, Alexander M. Aravanis, Sonya Parpart-Li, Nathan Hunkapiller
Summary: This article reports on the analytical validation of a targeted methylation-based cell-free DNA multi-cancer early detection test. The test showed high sensitivity in detecting cancer samples and accurately predicted the tissue of origin for the cancer signal. No cross-contamination events were observed and the test was not affected by potential interferents.
Review
Oncology
Ayanthi A. Wijewardene, Marthe Chehade, Matti L. Gild, Roderick J. Clifton-Bligh, Martyn Bullock
Summary: Liquid biopsies are a novel technique that assess for circulating tumor cells (CTC), circulating tumor DNA (ctDNA), and microRNA in peripheral blood samples of cancer patients. The diagnostic role of liquid biopsy in oncology has expanded in recent years, especially in lung, colorectal, and breast cancer. In thyroid cancer, liquid biopsy's role in diagnosis or prognosis is transitioning from the lab to clinical practice.
Article
Medical Laboratory Technology
Ymke van der Pol, Norbert Moldovan, Sandra Verkuijlen, Jip Ramaker, Dries Boers, Wendy Onstenk, Johan de Rooij, Idris Bahce, D. Michiel Pegtel, Florent Mouliere
Summary: This study analyzed the impact of collection tube, plasma processing time, and physiology on cfDNA, and found that different collection tubes and processing times did not affect the fragment sizes of cfDNA, but could impact the genome-wide fragmentation patterns and fragment-end sequences. Additionally, physiological conditions minimally influenced the outcome of cfDNA fragmentomic methods.
CLINICAL CHEMISTRY
(2022)
Article
Oncology
Claudia Koch, Simon A. Joosse, Svenja Schneegans, Okka J. W. Wilken, Melanie Janning, Desiree Loreth, Volkmar Mueller, Katharina Prieske, Malgorzata Banys-Paluchowski, Ludwig J. Horst, Sonja Loges, Sven Peine, Harriet Wikman, Tobias M. Gorges, Klaus Pantel
Article
Biochemistry & Molecular Biology
Francesco Pantano, Martine Croset, Keltouma Driouch, Natalia Bednarz-Knoll, Michele Iuliani, Giulia Ribelli, Edith Bonnelye, Harriet Wikman, Sandra Geraci, Florian Bonin, Sonia Simonetti, Bruno Vincenzi, Saw See Hong, Sofia Sousa, Klaus Pantel, Giuseppe Tonini, Daniele Santini, Philippe Clezardin
Summary: This study identified ITGA5 as a mediator of breast-to-bone metastasis, with high expression correlating with poor bone metastasis-free survival. Silencing ITGA5 impaired tumor cell functions and bone metastasis, while inhibition with M200 showed similar effects.
Article
Biochemistry & Molecular Biology
Desiree Loreth, Moritz Schuette, Jenny Zinke, Malte Mohme, Andras Piffko, Svenja Schneegans, Julia Stadler, Melanie Janning, Sonja Loges, Simon A. Joosse, Katrin Lamszus, Manfred Westphal, Volkmar Mueller, Markus Glatzel, Jakob Matschke, Christoffer Gebhardt, Stefan W. Schneider, Iwona Belczacka, Beate Volkmer, Ruediger Greinert, Marie-Laure Yaspo, Patrick N. Harter, Klaus Pantel, Harriet Wikman
Summary: CTCs can serve as a disease surrogate for brain metastases in lung, melanoma, and breast cancer patients, with CD44 and CD74 expression as prognostic markers. Detection of CTCs using a size-based microfluidic approach and a semi-automated cell recognition system allows further characterization of tumor cells possibly derived from brain metastases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Maha Elazezy, Sandra Schwentesius, Luisa Stegat, Harriet Wikman, Stefan Werner, Wael Y. Mansour, Antonio Virgilio Failla, Sven Peine, Volkmar Mueller, Jean Paul Thiery, Majid Ebrahimi Warkiani, Klaus Pantel, Simon A. Joosse
Summary: This study reveals that keratin 16 is a critical protein promoting cancer metastasis, associated with epithelial-mesenchymal transition (EMT) and positive regulation of cell motility, with its expression in circulating tumor cells correlating with shorter relapse-free survival in patients. Therefore, keratin 16 could serve as a prognostic marker to identify cancer patients at higher risk of metastasis.
Article
Oncology
Laura Brylka, Katharina Jaehn-Rickert, Anke Baranowsky, Mona Neven, Michael Horn, Timur Yorgan, Harriet Wikman, Stefan Werner, Andreas Luebke, Michael Amling, Bjoern Busse, Klaus Pantel, Thorsten Schinke
Summary: Breast cancer cells often metastasize to bone, where their interaction with bone remodeling cell types enhances osteolytic bone destruction. By injecting breast cancer cells into immunocompromised mice, it was found that there were more tumor cells in the spine compared to femora, and the presence of a transgene did not affect their spreading and metastatic outgrowth.
Article
Biochemistry & Molecular Biology
Outi Huttala, Desiree Loreth, Synnove Staff, Minna Tanner, Harriet Wikman, Timo Ylikomi
Summary: Vascularization plays a crucial role in the tumor microenvironment. This study focuses on decellularized in vitro capillaries and their impact on cancer cell growth patterns and drug responses. The researchers observed two distinct growth patterns - network and cluster - when cancer cells were grown on decellularized capillaries, with network formation associated with metastatic properties. Additionally, drug responses of patient-derived cells cultured on decellularized capillaries demonstrated better correlation with clinical findings compared to cells cultured on plastic.
Article
Oncology
Markus Leffers, Johanna Herbst, Jolanthe Kropidlowski, Katharina Prieske, Anna Lena Bohnen, Sven Peine, Anna Jaeger, Leticia Oliveira-Ferrer, Yvonne Goy, Barbara Schmalfeldt, Klaus Pantel, Linn Wolber, Katharina Effenberger, Harriet Wikman
Summary: In cervical cancer patients, the combination of CADM1 and MAL gene methylation as markers in liquid biopsy showed high sensitivity and specificity for discriminating cervical cancer from healthy controls. This liquid biopsy assay also demonstrated high sensitivity in stage I-II patients.
Review
Cell Biology
Thais Pereira-Veiga, Svenja Schneegans, Klaus Pantel, Harriet Wikman
Summary: The interaction between circulating tumor cells (CTCs) and blood cells plays a crucial role in metastatic progression and anti-metastatic therapies.
Article
Oncology
Christine Nitschke, Benedikt Markmann, Marie Tolle, Jolanthe Kropidlowski, Yassine Belloum, Mara R. Goetz, Hartmut Schluter, Marcel Kwiatkowski, Marianne Sinn, Jakob Izbicki, Klaus Pantel, Cenap Gungor, Faik G. Uzunoglu, Harriet Wikman
Summary: This study used a microfluidic-based approach to detect circulating tumor cells (CTCs) in patients with pancreatic ductal adenocarcinoma (PDAC), and assessed the expression of retinoic acid receptor responder 1 (RARRES1) on CTCs for the first time. The results showed that the detection rate of CTCs was higher during follow-up, and high CTC counts during follow-up in resected patients were associated with early tumor relapse. Combining CTC detection and RARRES1 expression could predict a worse prognosis in patients after curative operation during follow-up.
Article
Biochemistry & Molecular Biology
Christine Nitschke, Benedikt Markmann, Leonie Konczalla, Jolanthe Kropidlowski, Thais Pereira-Veiga, Pasquale Scognamiglio, Martin Schonrock, Marianne Sinn, Marie Tolle, Jakob Izbicki, Klaus Pantel, Faik G. Uzunoglu, Harriet Wikman
Summary: This study found that the detection of CAMLs can provide prognostic information in PDAC patients, particularly when combined with CTCs. This indicates the potential of liquid biopsy marker analyses.
Article
Biochemistry & Molecular Biology
Paul van der Leest, Melanie Janning, Naomi Rifaela, Maria L. Aguirre Azpurua, Jolanthe Kropidlowski, Sonja Loges, Nicolas Lozano, Alexander Sartori, Darryl Irwin, Pierre-Jean Lamy, T. Jeroen N. Hiltermann, Harry J. M. Groen, Klaus Pantel, Leon C. van Kempen, Harriet Wikman, Ed Schuuring
Summary: Analysis of ctDNA using a suitable diagnostic-grade platform can guide treatment decisions and monitor disease progression in cancer patients. This multicenter study evaluated the use of UltraSEEK(R) Lung Panel and MassARRAY(R) System for ccfDNA analysis in NSCLC patients, comparing it with ddPCR and tumor tissue NGS. The results showed high concordance between UltraSEEK(R) and ddPCR, as well as a correlation between ctDNA level decrease detected with UltraSEEK(R) and improved treatment outcomes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)