Article
Chemistry, Applied
Inal Bakhytkyzy, Weronika Hewelt-Belka, Agata Kot-Wasik
Summary: This study demonstrates a new method for comprehensive flaxseed lipidome profiling using LC-Q-TOF-MS and dispersive micro-solid-phase extraction. The method allows for simultaneous monitoring of both polar and non-polar lipids, overcoming the challenge of lipid species abundance.
Article
Neurosciences
Mayssa Hachem, Mirja Kaizer Ahmmed, Houda Nacir-Delord
Summary: Phospholipidomics is a specialized branch of lipidomics that provides valuable insights into the metabolism and activities of phospholipids in brain disorders. By identifying specific phospholipid biomarkers, it has the potential to enhance diagnosis and treatment options for neurological diseases.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Celine Dalle, Jeremy Tournayre, Malwina Mainka, Alicja Basiak-Rasala, Melanie Petera, Sophie Lefevre-Arbogast, Jessica Dalloux-Chioccioli, Melanie Deschasaux-Tanguy, Lucie Lecuyer, Emmanuelle Kesse-Guyot, Leopold K. Fezeu, Serge Hercberg, Pilar Galan, Cecilia Samieri, Katarzyna Zatonska, Philip C. Calder, Mads Fiil Hjorth, Arne Astrup, Andre Mazur, Justine Bertrand-Michel, Nils Helge Schebb, Andrzej Szuba, Mathilde Touvier, John W. Newman, Cecile Gladine
Summary: The oxylipin signature of patients with metabolic syndrome enhances MetS phenotyping and may ultimately help to better stratify the risk of cardiometabolic diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Chemical
Sze Ying Lee, Xiao Hu, David C. Stuckey
Summary: In this study, the extraction of menaquinone was investigated. Ethanol was found to be the most effective extractant, and the technique was optimized using response surface methodology. Furthermore, microwave assisted extraction was studied to enhance the extraction yield of menaquinone. The research proposes a sustainable and efficient single-step process for the recovery of menaquinone, with the advantage of using ethanol as a green extraction solvent.
SEPARATION AND PURIFICATION TECHNOLOGY
(2022)
Article
Chemistry, Analytical
Guisheng Zhou, Jiawei Lu, Tingting Xu, Yan Lu, Wenjun Chen, Jue Wang, Mengying Ke, Qiuxiang Shen, Youjuan Zhu, Jinjun Shan, Shijia Liu
Summary: Rheumatoid arthritis (RA) is a chronic inflammatory disorder characterized by synovial inflammation. Lipid profile changes are observed before RA symptoms, indicating lipid involvement in inflammation. The study identified 36 differential lipid metabolites, with specific biomarkers for RA diagnosis. The lipid biomarkers were associated with disease activity in RA, showing disrupted lipid peroxidation.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Applied
Daniela Couto, Tania Melo, Tiago A. Conde, Ana S. P. Moreira, Paula Ferreira, Margarida Costa, Joana Silva, Rosario Domingues, Pedro Domingues
Summary: This study evaluated the efficiency of lipid extraction from Chlorella vulgaris and compared the effects of food-grade ethanol, ultrasound-assisted ethanol, and dichloromethane/methanol. The results showed that ultrasound-assisted ethanol and dichloromethane/methanol had the highest extraction yields, and the fatty acid profiles were identical among all extracts. However, the polar lipidome of the ethanol extracts was significantly different from the other two extracts. These findings are important for obtaining high yields of omega-3 and omega-6-rich extracts from C. vulgaris for food applications.
Article
Biochemical Research Methods
Jakub Idkowiak, Robert Jirasko, Denisa Kolarova, Josef Bartl, Tomas Hajek, Michela Antonelli, Zuzana Vankova, Denise Wolrab, Roman Hrstka, Hana Studentova, Bohuslav Melichar, Karolina Peskova, Michal Holcapek
Summary: Direct infusion of lipid extracts is commonly used in lipid analysis. Flow injection analysis (FIA) provides a simple alternative to shotgun-based approaches. The modified FIA-MS/MS method is robust and suitable for lipid quantitation in human plasma and has potential for clinical applications.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Camillo Morano, Gabriella Roda, Rita Paroni, Michele Dei Cas
Summary: In this study, we evaluated the performance of three different single-phase extraction methods for untargeted lipidomics analysis. We found that a methanol/chloroform mixture produced the most satisfactory results. In addition, we developed a filtration method called tip-tip filtration, which improved sample purity and mass compatibility. This method not only increased reproducibility, but also saved time and money.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Qingqing Wang, Miriam Hoene, Chunxiu Hu, Louise Fritsche, Robert Ahrends, Gerhard Liebisch, Kim Ekroos, Andreas Fritsche, Andreas L. Birkenfeld, Xinyu Liu, Xinjie Zhao, Qi Li, Benzhe Su, Andreas Peter, Guowang Xu, Rainer Lehmann
Summary: Reliability, robustness, and interlaboratory comparability of quantitative measurements in clinical lipidomics studies are crucial. The different ex vivo stability of lipids in blood poses a risk to data interpretation. Clear recommendations for blood sample collection are necessary. In this study, we used UHPLC-high resolution mass spectrometry to investigate the stability of 417 lipid species in EDTA whole blood under different temperature conditions and time points. Our data suggest that cooling whole blood immediately and continuously, and separating plasma within 4 hours, unless focusing on robust lipids, are recommended. Lists of lipids' ex vivo (in)stability and potential biomarkers in whole blood are provided. Overall, our results contribute to the international efforts in achieving reliable and comparable clinical lipidomics studies in the future.
JOURNAL OF LIPID RESEARCH
(2023)
Article
Food Science & Technology
Silvia Guazzotti, Cristina Pagliano, Francesco Dondero, Marcello Manfredi
Summary: This study evaluated the lipidomic profile of rice bran from the Italian rice variety, Roma, using ultra performance liquid chromatography-tandem mass spectrometry. Ethanol and methyl tert-butyl ether/methanol solvents were found to be excellent solvents for lipid extraction from rice bran, with ethanol being the most effective for triglycerides and N-acylethanolamines, and methyl tert-butyl ether/methanol being the most effective for diglycerides, phospholipids, and ceramides. Additionally, extraction with ethanol at 20 degrees C yielded similar results to extraction at 4 degrees C. Therefore, ethanol and methyl tert-butyl ether/methanol can be used to efficiently extract lipids from rice bran, contributing to the valorization of this by-product in a circular economy perspective.
Article
Engineering, Chemical
Nor Qhairul Izzreen Mohd Noor, Rina Syafinaz Razali, Nur Khairina Ismail, Rabiatul Amirah Ramli, Umi Hartina Mohamad Razali, Ahmad Riduan Bahauddin, Nazikussabah Zaharudin, Ashari Rozzamri, Jamilah Bakar, Sharifudin Md. Shaarani
Summary: Growing demand for green and sustainable processing has led to the application of novel extraction technologies for gelatin in the food industry. This review discusses the principles and potential applications of green technologies, as well as factors affecting gelatin quality and various extraction methods. Technologies such as UAE, subcritical water extraction, high-pressure processing, and MAE have shown promise in improving gelatin extraction efficiency and reducing environmental impact.
Article
Biochemistry & Molecular Biology
Ashraf M. Omar, Qibin Zhang
Summary: Lipidomics is the process of fully characterizing lipids in a cell, tissue, organism, or biological system. The extraction of lipids from biological samples is a bottleneck in reliable lipidomic analysis. Different extraction methods have varying lipid recovery and reproducibility. The Folch method is the most effective and reproducible for extracting lipids from mouse pancreas, spleen, brain, and plasma, while the MMC and BUME methods are preferred for extracting mouse liver or intestine.
Review
Chemistry, Analytical
Blanca H. Lapizco-Encinas
Summary: This review article discusses the latest advancements in microscale electrokinetics for the analysis of cells and subcellular materials in clinical applications. It covers the potential of electrokinetic-based methods in liquid biopsies, circulating tumor cells, blood components, stem cells, and subcellular particles. The conclusions summarize the main advancements and future prospects in this field.
Article
Biochemical Research Methods
Ahmed M. M. Abdel-Megied, Isaac A. A. Monreal, Limian Zhao, Alex Apffel, Hector C. C. Aguilar, Jace W. W. Jones
Summary: Emerging and re-emerging zoonotic viral diseases, particularly enveloped viruses like SARS-CoV-2, have a significant impact on public health. The lipid composition of the viral envelope and changes in host cell lipid metabolism during viral infection provide an opportunity to understand how these viruses infect cells. In this study, the lipid composition of VeroE6-TMPRSS2 cells infected with SARS-CoV-2 was characterized, with a focus on the ceramide to sphingomyelin ratio. This lipid profile can help in the development of antiviral strategies and vaccines.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Acoustics
Xue Li, Baokun Qi, Shuang Zhang, Yang Li
Summary: This study found that cactus fruit seed oil (CFO) extracted using traditional solvent is rich in linolenic acid, α-tocopherol, and canolol. Ultrasound-assisted extraction can increase the content of lipid concomitants in CFO, but excessive intensity may cause oxidation of oils. Further analysis showed that CFO has the ability to regulate lipid metabolism balance.
ULTRASONICS SONOCHEMISTRY
(2023)
Article
Chemistry, Analytical
Marc Wolter, Christian Geibel, Matthias Olfert, Min Su, Wolfgang Bicker, Markus Kramer, Wolfgang Lindner, Michael Laemmerhofer
Summary: This study reports a novel stable-bonded amino silica stationary phase obtained by crosslinking surface aminopropyl moieties. The new phase exhibited superior hydrolytic stability and showed excellent separation performance in the separation of therapeutically effective sulfonamides and carbohydrates.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Chemistry, Analytical
Peng Li, Min Su, Madhumita Chatterjee, Michael Lammerhofer
Summary: This article introduces an improved workflow for monitoring metabolites in the glycolysis pathway. The method is validated in different biological samples and shows application in platelets. Additionally, it can be extended to the analysis of phosphorylated and carboxylated metabolites.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biochemical Research Methods
Simon Jaag, Chunmei Wen, Benjamin Peters, Michael Lammerhofer
Summary: This study systematically tests 13 different reversed-phase columns using the gradient kinetic plot method. The results show that columns with different morphologies perform similarly for small proteins, but specific columns perform better for antibody fragments and intact antibodies.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Analytical
Peng Li, Michael Laemmerhofer
Summary: The study developed a method for generating U13C-inositol and U13C-IPx, successfully applicable in biological samples. The U13C analogs as internal standards in biological applications exhibit good stability and accuracy, effectively compensating for sample losses during preparation and matrix effects during ionization.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Kristian Serafimov, Michael Laemmerhofer
Summary: In this study, a targeted approach with wide metabolite coverage was developed for cellular metabolomic analysis using a UHPLC-QTrap-MS system operated in the scheduled multiple reaction monitoring (sMRM) mode. The study compared four different cell extraction protocols and developed two HILIC-MS methods. A total of 161 metabolites were successfully detected in ESI+ mode, and 92 were detected in negative ionization mode. The developed methods were calibrated and validated, showing good accuracy and precision.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biochemical Research Methods
Min Su, Kristian Serafimov, Peng Li, Cornelius Knappe, Michael Laemmerhofer
Summary: The chromatographic behavior of mixed-mode and hydrophilic interaction liquid chromatography (HILIC) was assessed in this study for the separation of metabolites from glycolysis and pentose phosphate pathways. Optimization of chromatographic conditions allowed for selective targeted analysis of glycolytic phosphorylated carbohydrate metabolites using liquid chromatography with tandem mass spectrometry (MS/MS). The separation of pentose phosphate isomers was achieved using mixed-mode HILIC/SAX and HILIC BEH Amide columns. HILIC-MS/MS showed challenges in separating hexose monophosphate metabolites, but the BEH Amide column provided the best results for this separation.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemical Research Methods
Feiyang Li, Shenkai Chen, Sylwia Studzinska, Michael Laemmerhofer
Summary: Ion-pairing reversed-phase liquid chromatography (IP-RPLC) is commonly used for the analysis of nucleic acid related compounds. However, the high concentration of ion-pairing reagent in IP-RPLC is not suitable for coupling with mass spectrometry (MS). This study explored the use of a PBT-bonded silica-based stationary phase for the separation of Patisiran without the need for ion-pairing reagents. The method allowed for MS and MS/MS characterization without contamination or related issues.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemical Research Methods
Ulrich Woiwode, Adrian Sievers-Engler, Michael Laemmerhofer
Summary: In this work, new polymeric chiral stationary phases were prepared and compared with brush-type chiral stationary phases. The polymeric CSPs showed similar enantioselectivity but lower chromatographic efficiencies compared to the brush phases. Oxidation of the polymeric CSPs reduced retention times and allowed for lower counterion concentrations in liquid chromatography-electrospray ionization-mass spectrometry applications. In addition, cryogenic cooling of the column enabled baseline separation of estradiol.
Article
Biochemical Research Methods
Xiaoqing Fu, Nourhane Hafza, Friedrich Goetz, Michael Laemmerhofer
Summary: In this study, seven commercially available UHPLC columns were systematically evaluated for their retention and isomer selectivity of branched chain fatty acids (BCFAs). It was found that IG-U column showed the best isomer selectivity for short and medium-chain BCFA isomers, while RP C18 column had good selectivity for long-chain BCFA isomers. Furthermore, an untargeted isomer-selective BCFA profiling method was developed using UHPLC-ESI-MS/MS without pre-column derivatization.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemical Research Methods
Feiyang Li, Ryan Karongo, Despoina Mavridou, Jeannie Horak, Adrian Sievers-Engler, Michael Laemmerhofer
Summary: Enantioselective amino acid analysis is an important technique in pharmaceutical, biomedical, and food sciences. However, simultaneously analyzing all chiral proteinogenic amino acids using a single method with one column and one condition is still challenging. In this study, an enantioselective high-performance liquid chromatography-tandem mass spectrometry (LC-MS) assay using Chiralpak QN-AX as a chiral column was developed, enabling efficient separation of D- and L-amino acids.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemical Research Methods
Feiyang Li, Shenkai Chen, Sylwia Studzinska, Michael Laemmerhofer
Summary: With the increasing use of nucleic acid-based pharmaceuticals, efficient analytical methods are needed for synthetic oligonucleotides. Two-dimensional chromatography techniques, including orthogonal chromatographic methods, are becoming more relevant due to the complexity of the oligonucleotides. In this study, a new polybutylene terephthalate-based stationary phase was tested for the analysis of siRNA, showing higher orthogonality and improved resolution for peak purity evaluation.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Medicinal
Sebastian Vaas, Markus O. Zimmermann, Dieter Schollmeyer, Jason Stahlecker, Marc U. Engelhardt, Janosch Rheinganz, Bernhard Drotleff, Matthias Olfert, Michael La''mmerhofer, Markus Kramer, Thilo Stehle, Frank M. Boeckler
Summary: This article highlights the usefulness of CF2X (X = Cl, Br, or I) moieties as an orthogonal principle to the established (hetero)arylhalides. The chemical/metabolic stability, logP/solubility, and crystal structures of compounds bearing CF2X groups are studied. The interactions, conformational flexibilities, and XB donor capacities of CF2X moieties are analyzed. Furthermore, the implementation of CF2X acetamides in molecular design and their binding modes in JNK3 are investigated.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Ryan Karongo, Jeannie Horak, Michael Laemmerhofer
Summary: This work presents a comprehensive achiral x chiral two-dimensional liquid chromatography separation method for enantioselective amino acid analysis. It utilizes flow splitting and a multi-detector approach for volumetric flow reduction and efficient ionization. The method overcomes limitations of chiral columns and can be used for complex sample mixtures. It has been successfully applied to control the stereointegrity of the therapeutic peptide octreotide.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Chemistry, Analytical
Kristian Serafimov, Yuesra Aydin, Michael Laemmerhofer
Summary: In this study, a fast-targeted liquid chromatography-tandem mass spectrometry method was proposed for accurate analysis of metabolites from the glutathione pathway. The addition of N-Ethylmaleimide reagent to the extraction solvent quickly stabilized the thiol-redox state. This method demonstrated high throughput analysis, sensitivity, and accuracy.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Chemistry, Analytical
Yujia Ying, Huilin Li
Summary: Enzymes play a crucial role as biological catalysts in accelerating biochemical reactions in living organisms. This study proposes a convenient method for monitoring enzymatic catalytic processes using native top-down mass spectrometry. By exploring the heterogeneity of the chymotrypsin sample and using tandem mass spectrometry, the researchers were able to monitor covalent and noncovalent enzymatic complexes, substrates, and products during catalysis. The results demonstrate that this method has the potential to be a promising tool for characterizing biocatalysts.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Kang Yang, Shuaibo Shi, Jinyu Wu, Shaolong Han, Shengdi Tai, Shishen Zhang, Kun Zhang
Summary: H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Julia Kuligowski, Alvaro Perez-Rubio, Marta Moreno-Torres, Polina Soluyanova, Judith Perez-Rojas, Ivan Rienda, David Perez-Guaita, Eugenia Pareja, Ramon Trullenque-Juan, Jose Castell, Marcha Verheijen, Florian Caiment, Ramiro Jover, Guillermo Quintas
Summary: This study introduces a novel variable selection approach called cluster PLS (c-PLS) to assess the joint impact of variable groups selected based on biological characteristics on the predictive performance of a multivariate model. The usefulness of c-PLS is shown using miRNomic and metabolomic datasets obtained from the analysis of liver tissue biopsies.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Meixue Lai, Lijie Zhong, Siyi Liu, Yitian Tang, Tingting Han, Huali Deng, Yu Bao, Yingming Ma, Wei Wang, Li Niu, Shiyu Gan
Summary: This study presents a method for constructing wearable sweat electrolyte sensors using carbon fiber-based solid-contact ion-selective electrodes (SC-ISEs). By using carbon fibers extracted from commercial cloth as electrode material, the cost and reproducibility issues of flexible SC-ISEs were addressed. The results showed that the carbon fiber-based SC-ISEs exhibited reversible voltammetric and stable impedance performances, and had high reproducibility of standard potentials between normal and bending states.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Yang, Ying Liu, Min Deng, Peipei Wang, Dan Cheng, Songjiao Li, Longwei He
Summary: A near-infrared fluorescent probe has been developed to accurately measure the levels of peroxynitrite (ONOO-) in the endoplasmic reticulum (ER) in acute lung injury (ALI). The probe demonstrated rapid response, high selectivity, good sensitivity, and enhanced fluorescence intensity in response to ONOO-. It was successfully used to detect changes in ONOO- levels and showed significant increases in an ALI cell model and an ALI mouse model.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yanping Wang, Yuemei Chen, Kejun Li, Jinrong Zhou, Xin Yuan, Mei Zhang, Ke Huang
Summary: In this work, a portable analytical system based on point discharge chemical vapor generation atomic emission spectrometry (PD-CVG-AES) coupling with gold filament enrichment was designed. The highly sensitive analysis of Hg2+ indirectly realized the detection of ascorbic acid (AA). The measurement is based on the fact that Ag+ can decrease the concentration of Hg2+ by forming Ag-Hg amalgam in the presence of the reductant SnCl2, while AA can pre-reduce Ag+ to Ag0, leading to the generation of silver nanoparticles (Ag NPs). The developed novel analytical strategy broadens the application of microplasma-based AES and offers a higher level of sensitivity compared to current AA detection techniques.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Wei Lang, Jia-Mei Qin, Qian-Yong Cao
Summary: A novel polymer-based probe P1 was successfully synthesized for fluorescently ratiometric sensing of H2S with high selectivity and sensitivity. A smartphone sensing platform was constructed to conduct visual quantitative detection of H2S. P1 can be employed in evaluating the level fluctuations of H2S in living cells, testing water samples/wine samples, and monitoring food freshness.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Siriwan Teepoo, Jongjit Jantra, Khaunnapa Panapong, David Taiwo Ajayi
Summary: A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Xiaohan Zhao, Anyu Wang, Lingzi Zhai, Jiuhe Gao, Sizhe Lyu, Yingshan Jiang, Tian Zhong, Ying Xiao, Xi Yu
Summary: A novel method using polystyrene-coated magnetic nanoparticles for extracting monohydroxy polycyclic aromatic hydrocarbons from urine samples was investigated. The proposed method is simple, sensitive, and efficient, with desirable sensitivity for analyzing low-abundance metabolites in large volumes of complex urine samples.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Dongjuan Wang, Xiuqian Ding, Jinling Xie, Juan Wang, Guanhao Li, Xin Zhou
Summary: In this study, a three-in-one sensor was developed for real-time detection of biogenic amines (BAs) with high sensitivity and selectivity. The sensor showed multimodal responses and could be used to fabricate portable devices for on-site non-destructive assessment of food spoilage indicators.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Jinting Meng, Zihao Xu, Shasha Zheng, Hongqun Yang, Tianfu Wang, Hong Wang, Yingwei Zhang
Summary: This study demonstrates the development of a cascade signal amplification system using a multi-pedal DNA walker and strand displacement reactions for the electrochemical detection of miRNA-155. The biosensor exhibited high sensitivity, selectivity, and the potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2024)