Journal
JOURNAL OF CLINICAL MICROBIOLOGY
Volume 54, Issue 8, Pages 2197-2200Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.01156-16
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Funding
- Health Department of the Basque Country Government [2009111012]
- University of the Basque Country EHU/UPV [IT656-13]
- MINECO \ Instituto de Salud Carlos III (ISCIII) [PI13/01708]
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For pneumococcal disease surveillance, simple and cost-effective methods capable of determining all serotypes are needed. Combining a single-tube multiplex PCR with fluorescently labeled primers followed by amplicon analysis using automated fluorescent capillary electrophoresis, each serotype of 92 reference isolates and 297 recently collected clinical isolates was successfully determined.
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