Article
Biochemistry & Molecular Biology
Yu-Jen Chen, Chia-Tien Hsu, Shang-Feng Tsai, Cheng-Hsu Chen
Summary: Chronic allograft dysfunction (CAD) is a major condition affecting long-term kidney graft survival. In this study, the correlation between plasma miR-21 and the severity of CAD was investigated. RNA sequencing was performed and differentially expressed miRNAs in CAD were identified. MiR-21-5p, miR-20a-5p, and miR-101-3p were found to be associated with the TGF-beta pathway and CAD. These findings may lead to the development of early prediction miRNA biomarkers and therapeutic tools for CAD in the field of kidney transplantation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Chairmandurai Aravindraja, Syam Jeepipalli, Krishna Mukesh Vekariya, Ruben Botello-Escalante, Edward K. L. Chan, Lakshmyya Kesavalu
Summary: This study aimed to investigate the global miRNA expression kinetics during the progression of periodontitis in mice infected with T. denticola. The results showed that T. denticola infection led to bacterial colonization and increased alveolar bone resorption. A total of 70 miRNAs with differential expression were identified, and miR-486, miR-126-3p, and miR-126-5p were found to be potential invasive biomarkers of T. denticola in periodontal disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Michele Manganelli, Ilaria Grossi, Manuela Ferracin, Paola Guerriero, Massimo Negrini, Michele Ghidini, Chiara Senti, Margherita Ratti, Claudio Pizzo, Rodolfo Passalacqua, Sarah Molfino, Gianluca Baiocchi, Nazario Portolani, Eleonora Marchina, Giuseppina De Petro, Alessandro Salvi
Summary: The study investigated the longitudinal levels of cell-free long ncRNA GAS5 and microRNAs miR-126-3p and miR-23b-3p in patients with hepatocellular carcinoma (HCC) undergoing sorafenib treatment. The results highlighted the significant downregulation of miR-23b-3p in untreated HCC patients and its diagnostic relevance.
Article
Oncology
Chiara Verdelli, Irene Forno, Annamaria Morotti, Riccardo Maggiore, Gilberto Mari, Leonardo Vicentini, Stefano Ferrero, Elisabetta Kuhn, Valentina Vaira, Sabrina Corbetta
Summary: Tumors of the parathyroid glands display a distinct miRNA profile from normal glands, with miR-126-3p downregulated in parathyroid adenomas. Localization of miR-126-3p to endothelial cells and tumor-associated myofibroblasts suggests its role in promoting neoangiogenesis through VEGFA overexpression.
ENDOCRINE-RELATED CANCER
(2021)
Article
Cell Biology
Kevin Bassand, Laurent Metzinger, Meriem Naim, Nesrine Mouhoubi, Oualid Haddad, Vincent Assoun, Naima Zaidi, Odile Sainte-Catherine, Amena Butt, Erwan Guyot, Olivier Oudar, Christelle Laguillier-Morizot, Angela Sutton, Nathalie Charnaux, Valerie Metzinger-Le Meuth, Hanna Hlawaty
Summary: The study revealed the significant role of miR-126 in CXCL12-induced angiogenesis, with inhibition of miR-126-3p affecting cell angiogenesis and migration capabilities. Furthermore, SPRED-1, a target of miR-126-3p, plays a crucial role in the process of angiogenesis.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Nina P. Jordan, Samuel J. Tingle, Victoria G. Shuttleworth, Katie Cooke, Rachael E. Redgrave, Esha Singh, Emily K. Glover, Hafiza B. Ahmad Tajuddin, John A. Kirby, Helen M. Arthur, Chris Ward, Neil S. Sheerin, Simi Ali
Summary: The down-regulation of miR-126-3p is associated with EndMT, with its over-expression preventing the process and maintaining endothelial characteristics while repressing fibronectin expression. Lineage tracing in cardiac and kidney fibrosis models revealed that a significant subpopulation of endothelial-derived cells undergo EndMT and express mesenchymal markers. MiR-126-3p expression is restricted to endothelial cells and down-regulated in fibrotic kidney and heart tissue, making it a potential target for miRNA-based therapeutics in fibrotic organs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, Research & Experimental
Peng Zeng, Jie Yang, Lipei Liu, Xiaoxiao Yang, Zhi Yao, Chuanrui Ma, Haibo Zhu, Jiamin Su, Qian Zhao, Ke Feng, Shu Yang, Yan Zhu, Xiaoju Li, Wenguang Wang, Yajun Duan, Jihong Han, Yuanli Chen
Summary: The study showed a positive correlation between calcification in human aortic valves and ERK1/2 activity. Inhibition of ERK1/2 reduced atherosclerosis and calcification in animal models, as well as calcification in SMCs and arterial rings. The reduction of calcification was due to decreased expression of ALP, BMP2, and RUNX2, and activation of DKK1 and LRP6 expression, thereby inactivating Wnt signaling pathways in SMCs.
Article
Medical Laboratory Technology
Yahui Shen, Huiyu Lu, Guixian Song
Summary: The study found that COPD patients had higher levels of miR-221-3p and miR-92a-3p in their serum compared to healthy volunteers, which were effective biomarkers in diagnosing stable COPD from acute exacerbation COPD. Exposure to cigarette smoke extract (CSE) weakened the viability of 16HBECs and led to increased release of inflammatory cytokines. Suppression of miR-221-3p/miR-92a-3p expression in 16HBECs after transfection with inhibitors reduced CSE-induced inflammation, suggesting these miRNAs as potential diagnostic markers for COPD patients with a smoking history.
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2021)
Article
Medicine, Research & Experimental
Jizi Zhou, Ying Zhao, Ping An, Huanqiang Zhao, Xiaotian Li, Yu Xiong
Summary: This study reveals that circRNA hsa_circ_0002348 is significantly increased in preeclampsia placentas and correlates with the severity of clinical manifestations. It functions as an endogenous sponge for miR-126-3p to upregulate BAK1 expression, thereby regulating trophoblast proliferation and apoptosis. Hsa_circ_0002348 may serve as a potential therapeutic target for preeclampsia.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Medicine, Research & Experimental
Xue Zhang, Peng Wang, Kai Yuan, Maoran Li, Yiting Shen, Huafa Que, Yunfei Wang, Wei Liang
Summary: The circular RNA hsa_circ_0024093 from YAP1 was found to positively regulate YAP1 protein levels in VSMCs, affecting cell proliferation, migration, and apoptosis in the in vitro model of ASO of the lower extremities. Mechanistically, hsa_circ_0024093 was shown to regulate YAP1 protein expression by sequestering miR-889-3p or miR-4677-3p to enhance USP9X expression. Rescue assays further confirmed the regulatory effects of hsa_circ_0024093 on VSMC function in ASO of the lower extremities. These findings offer new insights into the pathogenesis of ASO.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2021)
Article
Health Care Sciences & Services
Milan Hromadka, Zuzana Motovska, Ota Hlinomaz, Petr Kala, Frantisek Tousek, Jiri Jarkovsky, Marketa Beranova, Pavel Jansky, Michal Svoboda, Iveta Krepelkova, Richard Rokyta, Petr Widimsky, Michal Karpisek
Summary: This study indicated that miR-223-3p and miR-126-3p are promising independent predictors of thrombotic events post-AMI, with miR-223-3p levels significantly associated with ischemic endpoints and the miR-126-3p to miR-223-3p ratio being related to risk reduction.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Medicine, General & Internal
Olga Martinez-Arroyo, Ana Ortega, Ana Flores-Chova, Belen Sanchez-Garcia, Ana B. Garcia-Garcia, Felipe J. Chaves, Juan C. Martin-Escudero, Maria Jose Forner, Josep Redon, Raquel Cortes
Summary: Elevated plasma microRNA levels, particularly miR-126-3p, are associated with albuminuria in hypertensive patients, and may serve as prognostic markers for cardiovascular events. Further studies are needed to assess the potential role of miR-126-3p in evaluating endothelial dysfunction.
EUROPEAN JOURNAL OF INTERNAL MEDICINE
(2023)
Article
Multidisciplinary Sciences
Xinyang Shou, Yimin Wang, Qingyu Jiang, Jun Chen, Qiang Liu
Summary: The study found that miR-126 can inhibit ox-LDL-induced foam cell formation and phagocytosis, and promote the conversion of pro-inflammatory M1 macrophages to anti-inflammatory M2 macrophages. In addition, miR-126 can reduce the expression of vascular endothelial growth factor A (VEGFA) and transcriptional regulator Kruppel-like factor 4 (KLF4). These findings suggest that miR-126 plays an important role in atherosclerosis.
Article
Oncology
Daniela Di Paolo, Francesca Pontis, Massimo Moro, Giovanni Centonze, Giulia Bertolini, Massimo Milione, Mavis Mensah, Miriam Segale, Ilaria Petraroia, Cristina Borzi, Paola Suatoni, Chiara Brignole, Patrizia Perri, Mirco Ponzoni, Ugo Pastorino, Gabriella Sozzi, Orazio Fortunato
Summary: Simultaneously targeting miR-126-3p and miR-221-3p to inhibit tumor growth and metastasis could be a novel therapeutic approach for lung cancer management.
MOLECULAR ONCOLOGY
(2021)
Article
Endocrinology & Metabolism
Wen Lu, Xuan Du, Shengyi Zou, Qionglei Fang, Mengjiao Wu, Huijuan Li, Bimin Shi
Summary: This study demonstrates that exosomes secreted by IFN-γ-pretreated BMSCs (IExos) have enhanced therapeutic efficacy in diabetic wound healing compared to exosomes without IFN-γ treatment (NExos), by promoting angiogenesis. Specifically, exosomal miR-126-3p secreted from IFN-γ-pretreated BMSCs promotes angiogenesis and diabetic wound healing via the SPRED1/Ras/Erk pathway.
JOURNAL OF DIABETES
(2023)
Review
Endocrinology & Metabolism
Shuhui Zheng, Lin Wu, Chao Fan, Jingxia Lin, Yaxing Zhang, Tommaso Simoncini, Xiaodong Fu
Summary: G alpha plays a crucial role in mER-mediated signaling, involving processes such as vascular EC migration, endothelial H2S release, breast cancer cell invasion, neural regulation of energy balance, and osteogenesis.
GYNECOLOGICAL ENDOCRINOLOGY
(2021)
Editorial Material
Endocrinology & Metabolism
Rossella E. Nappi, Tommaso Simoncini
LANCET DIABETES & ENDOCRINOLOGY
(2021)
Review
Pharmacology & Pharmacy
Andrea R. Genazzani, Patrizia Monteleone, Andrea Giannini, Tommaso Simoncini
Summary: Personalized treatment is key to managing menopausal symptoms, with different drugs having distinct pharmacological and clinical properties. Physicians must have a deep understanding of the pharmacology of compounds to adjust therapy to fit the individual patient's characteristics and needs.
EXPERT OPINION ON PHARMACOTHERAPY
(2021)
Editorial Material
Endocrinology & Metabolism
Marta Caretto, Tommaso Simoncini
GYNECOLOGICAL ENDOCRINOLOGY
(2021)
Article
Surgery
Andrea Panattoni, Andrea Giannini, Riccardo Morganti, Paolo Mannella, Alessandra Perutelli, Vito Cela, Tommaso Simoncini
Summary: This preliminary study demonstrated the feasibility of ITM in performing da Vinci Xi TRH even in the first cases of surgery for malignancy such as early stage endometrial endometrioid cancer.
INTERNATIONAL JOURNAL OF MEDICAL ROBOTICS AND COMPUTER ASSISTED SURGERY
(2021)
Article
Immunology
Giorgio Bogani, Luca Lalli, Francesco Sopracordevole, Andrea Ciavattini, Alessandro Ghelardi, Tommaso Simoncini, Francesco Plotti, Jvan Casarin, Maurizio Serati, Ciro Pinelli, Alice Bergamini, Barbara Gardella, Andrea Dell'Acqua, Ermelinda Monti, Paolo Vercellini, Innocenza Palaia, Giorgia Perniola, Margherita Fischetti, Giusi Santangelo, Alice Fracassi, Giovanni D'Ippolito, Lorenzo Aguzzoli, Vincenzo Dario Mandato, Luca Giannella, Cono Scaffa, Francesca Falcone, Chiara Borghi, Mario Malzoni, Andrea Giannini, Maria Giovanna Salerno, Viola Liberale, Biagio Contino, Cristina Donfrancesco, Michele Desiato, Anna Myriam Perrone, Giulia Dondi, Pierandrea De Iaco, Simone Ferrero, Giuseppe Sarpietro, Maria G. Matarazzo, Antonio Cianci, Stefano Cianci, Sara Bosio, Simona Ruisi, Lavinia Mosca, Raffaele Tinelli, Rosa De Vincenzo, Gian Franco Zannoni, Gabriella Ferrandina, Marco Petrillo, Giampiero Capobianco, Salvatore Dessiole, Annunziata Carlea, Fulvio Zullo, Barbara Muschiato, Stefano Palomba, Stefano Greggi, Arsenio Spinillo, Fabio Ghezzi, Nicola Colacurci, Roberto Angioli, Pierluigi Benedetti Panici, Ludovico Muzii, Giovanni Scambia, Francesco Raspagliesi, Violante Di Donato
Summary: In this study, a prognostic nomogram was developed to assess the risk of cervical dysplasia persistence/recurrence after primary conization. Variables such as diagnosis of CIN3, presence of high-risk HPV types, positive endocervical margins, HPV persistence, and omission of HPV vaccination after conization were found to significantly increase the risk of cervical dysplasia persistence/recurrence. The nomogram can be a useful tool for counseling women about their risk and the importance of HPV vaccination after conization.
Article
Endocrinology & Metabolism
Alessandro D. Genazzani, Christian Battipaglia, Tabatha Petrillo, Nicola Piacquadio, Fedora Ambrosetti, Melania Arnesano, Elisa Semprini, Alessandra Sponzilli, Veronica Tomatis, Tommaso Simoncini
Summary: Polycystic ovary syndrome (PCOS) is a syndrome characterized by 2 out of 3 established criteria. Recently, insulin resistance (IR) has become a focus of attention. In this study, overweight/obese PCOS patients were treated with DCI and ALA for 3 months, and it was found that this treatment improved hormonal, metabolic profiles, and liver function.
GYNECOLOGICAL ENDOCRINOLOGY
(2022)
Review
Endocrinology & Metabolism
Alice Antonelli, Andrea Giannini, Peter Chedraui, Patrizia Monteleone, Marta Caretto, Alessandro D. Genazzani, Paolo Mannella, Tommaso Simoncini, Andrea R. Genazzani
Summary: Depressive disorders and anxiety states are common in women during vulnerable periods, and sex hormones play a key role in the development of these disorders by affecting neuronal plasticity and mood regulation.
GYNECOLOGICAL ENDOCRINOLOGY
(2022)
Article
Obstetrics & Gynecology
E. Russo, G. Misasi, M. M. Montt-Guevara, A. Giannini, T. Simoncini
Summary: This study aimed to evaluate the effects of ospemifene on OAB symptoms in postmenopausal women with vulvovaginal atrophy. The results showed that ospemifene treatment can reduce OAB symptoms, decrease bladder sensitivity, and improve quality of life.
Article
Obstetrics & Gynecology
S. Palacios, R. E. Nappi, M. J. Cancelo, S. Sanchez, T. Simoncini
Summary: Vulvovaginal atrophy (VVA) is a chronic condition in postmenopausal women, with underdiagnosis and undertreatment. Treatment options for moderate to severe VVA include local estrogens, dehydroepiandrosterone (DHEA), and oral ospemifene. A 5-year post-authorization safety study (PASS) was conducted to study the incidence of venous thromboembolism (VTE) in women receiving ospemifene. The results of the study led to regulatory changes in the labeling of ospemifene, expanding its indication and removing risk management measures.
Review
Obstetrics & Gynecology
Santiago Palacios
Summary: VVA is a chronic and progressive disease that affects sexuality and quality of life. It can be prevented and treated through long-term and sequential treatment. Current therapeutic options include over-the-counter topical products, hormone therapy, and prescribed medications. However, adherence is low due to concerns about efficacy, long-term estrogen use, and treatment cost.
Article
Obstetrics & Gynecology
Amerigo Ferrari, Giaele Moretti, Ilaria Corazza, Paolo Mannella, Tommaso Simoncini, Manila Bonciani
Summary: This study aims to assess the coverage of influenza and Tdap vaccines during pregnancy, explore predictors of vaccination related to socioeconomic and maternity pathways, and identify specific patterns of vaccination uptake. The results showed that pertussis vaccine had a higher coverage than influenza vaccine. High socioeconomic status, attending private gynecologists, and receiving vaccine information were the main predictors of vaccination. Three vaccination clusters were identified, and it is recommended that health workers and policymakers focus on pregnant women who are less likely to be vaccinated to promote vaccine information and encourage wider uptake.
INTERNATIONAL JOURNAL OF GYNECOLOGY & OBSTETRICS
(2023)
Article
Surgery
Tommaso Simoncini, Andrea Panattoni, Mustafa Aktas, Jozef Ampe, Cornelia Betschart, Alexander L. A. Bloemendaal, Stephan Buse, Giuseppe Campagna, Marta Caretto, Mauro Cervigni, Esther C. J. Consten, Hugo H. Davila, Jean Dubuisson, Eloy Espin-Basany, Bernardina Fabiani, Jean-Luc Faucheron, Andrea Giannini, Brooke Gurland, Dieter Hahnloser, Ralf Joukhadar, Paolo Mannella, Liliana Mereu, Jacopo Martellucci, Guillaume Meurette, Maria Magdalena Montt Guevara, Carlo Ratto, Barry A. O'Reilly, Christl Reisenauer, Eleonora Russo, Steven Schraffordt Koops, Shahab Siddiqi, Alessandro Sturiale, Gabriele Naldini
Summary: This study aimed to address the role of robotic pelvic floor reconstructive procedures through expert opinion. The results showed that robotic surgery has significant technical advantages over standard laparoscopy and is particularly important for complex reconstructive procedures, although benefits can be found in less challenging cases as well. However, more research is needed to objectively assess the specific settings where robotic surgery would provide the most benefit.
SURGICAL ENDOSCOPY AND OTHER INTERVENTIONAL TECHNIQUES
(2023)
Article
Obstetrics & Gynecology
Amerigo Ferrari, Paolo Mannella, Alessia Caputo, Tommaso Simoncini, Manila Bonciani
Summary: This prospective cohort study aimed to investigate the epidemiology of pregnancy-related urinary incontinence (UI) and its related risk factors, with a focus on women's characteristics related to maternity pathway utilization. The study utilized patient-reported data obtained from a systematic survey conducted in Tuscany, Italy. The results showed that the prevalence of UI ranged from 4.4% in the first trimester to 23.7% in the third trimester. Older, overweight/obese, and unemployed women had a higher occurrence and severity of UI. Factors such as high-risk pregnancy, discomfort during pregnancy, mode of delivery, and pregnancy examinations were found to be associated with the risk of UI. Performing pelvic-floor-muscle training during pregnancy was found to be protective against UI.
INTERNATIONAL JOURNAL OF GYNECOLOGY & OBSTETRICS
(2023)
Article
Psychology, Multidisciplinary
Lucia Ponti, Martina Smorti, Simon Ghinassi, Paolo Mannella, Tommaso Simoncini
Summary: This study explores the relationship between childbirth as a traumatic event and the quality of postnatal attachment, as well as the role of depressive symptomatology in this relationship. The findings indicate that postpartum stress symptoms have a positive impact on maternal depressive symptomatology and a negative impact on the quality of postnatal attachment. Additionally, a traumatic childbirth experience has both a direct and indirect effect on postnatal attachment, as it also influences the level of postnatal depression which, in turn, negatively affects the quality of the postnatal attachment bond.
CURRENT PSYCHOLOGY
(2022)