Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 1038, Issue -, Pages 12-18Publisher
ELSEVIER
DOI: 10.1016/j.jchromb.2016.10.017
Keywords
Trimethylamine N-oxide; Taurine; Plasma; Urine; Stability; Pre-analytical
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Background: Trimethylamine-N-oxide (TMAO) is produced in the liver from trimethylamine (TMA) and is an important cellular osmolyte and potential atherogenic factor. Taurine is involved in cholesterol metabolism and also serves as a cellular osmolyte. Given their significant biological functions, the development of reliable measurement techniques is crucial to further study their role in health and disease Methods: A new ultrahigh performance liquid chromatography-tandem mass spectrometry method was developed and validated for the simultaneous determination of TMA, TMAO, and taurine in plasma and urine. The method consisted of a deproteinization step using methanol/acetonitrile (15:85) that contained 0.2% formic acid and isotope -labeled internal standards. Samples were separated by centrifugation and injected into the UHPLC system. Quantification was conducted using a triple-quadrupole mass spectrometer detector with electrospray ionization interface in positive mode. Results: The limits of detection ranged from aos to 0.12 mu mol/L. The calibration curves were linear (r >= 0.999) over the range examined (0.15-400 mu mol/L) for all compounds. The inter-and intra-day coefficients of variations were <= 14.5% for TMA and <= 8% for TMAO and taurine. TMAO and taurine were found to be stable in EDTA plasma for at least 14 months at 70 degrees C. Mean recoveries ranged from 95% to 109% and the relative matrix effects were <= 4.0%. The method was applied to study physiological and pre-analytical factors in plasma and urine samples. Conclusions: The new UHPLC-MS/MS method has good accuracy, precision, and recovery. The assay combines simple sample processing with a short run time, making it well suited for high-throughput routine clinical or research purposes. (C) 2016 Elsevier B.V. All rights reserved.
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