Determination of albendazole sulfoxide in human plasma by using liquid chromatography-tandem mass spectrometry

A rapid, simple and sensitive method was developed and validated using liquid chromatography-tandem mass spectrometry (LC-MS/MS) for determination of albendazole sulfoxide (ABZOX) in human plasma. The plasma samples were extracted by protein precipitation using albendazole sulfoxide-d3 as internal standard (IS). The chromatographic separation was performed on Waters Xbridge C18Column (100 x 4.6 mm, 3.5 mu m) with a mobile phase consisting of ammonia solution, water and methanol at a flow rate of 0.70 mL/min. ABZOX was detected and identified by mass spectrometry with electrospray ionization (ESI) in positive ion and multiple-reaction monitoring (MRM) mode. The method was linear in the range of 3-1500 ng/mL for ABZOX. This method was successfully applied to the bioequivalence study in human plasma samples. (C) 2016 Elsevier B.V. All rights reserved.