Journal
JOURNAL OF ANALYSIS AND TESTING
Volume 6, Issue 2, Pages 120-128Publisher
SPRINGER SINGAPORE PTE LTD
DOI: 10.1007/s41664-022-00228-w
Keywords
Lateral flow strip; Split aptamer; Sandwich-type; AuNPs; Cocaine
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Funding
- National Natural Science Foundation of China [22090050, 22122410, 21874121]
- National Key Research and Development Program of China [2018YFE0206900]
- Hubei Provincial Natural Science Foundation of China [2020CFA037]
- Zhejiang Provincial Natural Science Foundation of China [LD21B050001]
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In this study, a sandwich-type lateral flow strip assay (LFSA) was developed using a strategy of splitting a single cocaine-recognizing aptamer into two segments. The LFSA showed excellent sensitivity and specificity for the detection of cocaine in urine and sweat samples, with a visual limit of detection as low as 50 μmol/L for urine and 200 μmol/L for sweat.
The sandwich-type lateral flow assays relying on dual aptamers with high sensitivity and specificity have been broadly explored. However, it is unlikely to match a pair of specific aptamers that can bind a small molecular target (e.g., cocaine) simultaneously due to the steric hindrance. In response, we herein introduced the strategy of one divides into two into the construction of sandwich-type lateral flow strip assay (LFSA). Specifically, we split a single cocaine-recognizing aptamer into two segments, either of which was conjugated with gold nanoparticles (AuNPs) or labeled with biotin, serving as signal probe and capture probe, respectively. Upon the presence of the target molecule, a ternary sandwich complex comprised of the two halves of the aptamer and the target formed. Such sandwich-type LFSA exhibited an excellent nonlinear logarithmic response in the range from 10 mu mol/L to 5 mmol/L with R-2 = 0.9994. The sensitive on-site detection of cocaine in artificial biological samples including urine and sweat was achieved within 15 min, with the visual limit of detection as low as 50 mu mol/L for urine and 200 mu mol/L for sweat, and the recovery rates of 83.6-107.4%.
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