Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 1031, Issue -, Pages 163-171Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2016.07.036
Keywords
Aloe; Response surface methodology; LC-ESI-MS/MS; Multivariate analysis
Funding
- Chungnam National University (Daejeon, Republic of Korea)
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Aloe arborescens, Aloe barbadensis and Aloe ferox are the most widely cultivated and used among 500 aloe species due to their potent bioactivity. However, the difference of aloe species is neglected and labeled only one name Aloe in the market without specifying aloe species discrimination in general. Furthermore, differences in bioactivity and side effects from different aloe species have not been well investigated. This study develops an effective method for simultaneous qualitative and quantitative determination of three common aloe species using high performance liquid chromatography-tandem mass spectrometry (HPLC MS/MS) and high performance liquid chromatography-diode array detection (HPLC-DAD). The extraction conditions were optimized by response surface methodology (RSM) based on methanol concentration, extraction time and solvent-to-material ratio. A partial least squares-discriminant analysis (PLS-DA) was used to identify the three aloe species. The developed HPLC MS/MS method coupled with multivariate analysis can be applied to discriminate three aloe species successfully. (C) 2016 Published by Elsevier B.V.
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