Article
Thermodynamics
Lin Liu, Mitsuhiro Kubota, Jun Li, Hayato Kimura, Yu Bai, Rongjun Wu, Lisheng Deng, Hongyu Huang, Noriyuki Kobayashi
Summary: Desiccant coated heat exchanger (DCHE) systems have significant potential in improving dehumidification performance. The study investigated the kinetics of silica gel and two aluminophosphate zeolites in an adsorber designed to simulate a DCHE air channel, showing that FAM Z05 had higher dehumidification performance at lower temperatures.
Review
Biochemical Research Methods
Xinyu Liu, Yu Wang, Hailin Cong, Youqing Shen, Bing Yu
Summary: Ion chromatography has made significant progress in the analysis field, with advantages such as speed, high sensitivity, and good selectivity. In recent years, new packing materials for ion chromatography with higher sensitivity and selectivity have been developed.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Applied
Salem Baidas, Khaled Al-Deyain, Xiaoguang Meng, Baoyu Gao
Summary: This study examined the competitive adsorption of perchlorate onto modified reed (MR) in the presence of phosphate and nitrate contaminants. The results showed that MR had a higher adsorption capacity for perchlorate alone, but the presence of phosphate had a stronger competitive effect on perchlorate adsorption. A multicomponent isotherm model was developed to describe the binary adsorption of perchlorate in the presence of competing anions.
ADSORPTION SCIENCE & TECHNOLOGY
(2023)
Article
Chemistry, Physical
Ningyi Chen, Chenhao Ni, Shuang Wu, Du Chen, Bingjun Pan
Summary: Phosphorus control is crucial for mitigating water eutrophication and addressing the scarcity of phosphorus resources. A novel nanocomposite, HNdO-PsAX, was developed for selective removal of phosphate. It exhibited stable and efficient phosphate adsorption over a wide pH range and outperformed the commercial lanthanum-based adsorbent, Phoslock. The specific Nd-P inner-sphere complexation in HNdO-PsAX contributed to its superior affinity towards phosphate. Fixed-bed treatment showed its high capacity for treating wastewater, and the feasibility of regeneration was recognized.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Nanoscience & Nanotechnology
Mojtaba Khanpour Matikolaei, Ehsan Binaeian
Summary: The study focused on a series of zinc-based metal-organic frameworks (MOFs) with different anions, showing that the CF3SO3- anion significantly enhances the ammonia uptake capacity of MOFs. Results indicate that MOFs can achieve high absorption capacity at low relative pressure, and retain good adsorption performance even after multiple regenerations.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Materials Science, Multidisciplinary
Tumisho E. Mabokela, Thabang R. Somo, Thabiso C. Maponya, Mpitloane J. Hato, Edwin Makhado, Katlego Makgopa, Kwena D. Modibane
Summary: In this study, the uptake capacity of CO2 by a metal organic framework (MOF) was investigated using simultaneous thermal analysis (STA) coupled with Fourier transform infrared spectroscopy (FTIR). The results showed that the MOF had the highest adsorption capacity at 25 degrees C and 2.1 bar pressure. The regenerability and structural stability of the MOF were tested over multiple sorption cycles and confirmed by time-based absorbance on FTIR. The combined intrinsic properties of the MOF make it a promising CO2 adsorbent for industrial applications.
Article
Chemistry, Analytical
Zongying Li, Xin Chen, Feifang Zhang, Bingcheng Yang
Summary: This study describes a strong anion exchanger used in ion chromatography, which is synthesized by grafting methacrylamido propyl trimethyl ammonium chloride onto hydrolyzed poly(glycidyl methacrylate-divinylbenzene) substrate. The exchanger exhibits obvious temperature-sensitive properties and large tolerance to samples with high ionic strength.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Thermodynamics
Alessio Sapienza, Vincenza Brancato, Yuri Aristov, Salvatore Vasta
Summary: In this study, plastic adsorbent heat exchangers were developed using 3D printing technology and experimentally tested for adsorption cooling applications. Results showed that the plastic adsorbers are competitive with metallic ones in terms of both thermodynamic and dynamic performance, with significant mass reduction and the ability to manufacture complex geometries. Under the tested operating conditions, the plastic adsorbers delivered a specific cooling power of 1.88-2.40 kW per kg of dry sorbent, while metallic adsorbers reached 2.34 kW/kg.
APPLIED THERMAL ENGINEERING
(2021)
Article
Chemistry, Multidisciplinary
Jie Li, Long Chen, Nannan Shen, Rongzhen Xie, Matthew Sheridan, Xijian Chen, Daopeng Sheng, Duo Zhang, Zhifang Chai, Shuao Wang
Summary: Tc-99 is a long-lived radionuclide present in large amounts in used nuclear fuel. Designing anion-exchange materials with optimized Tc uptake property is a research goal beneficial for reducing the volume of secondary radioactive solid waste. However, challenges exist in the design philosophy with difficulties such as charge repulsion, steric hindrance, and insufficient reactive sites.
SCIENCE CHINA-CHEMISTRY
(2021)
Article
Chemistry, Analytical
Gabriela Sanchez-Reyes, Heiner Graalfs, Mathias Hafner, Christian Frech
Summary: Ion exchange chromatography is a commonly used unit operation for the purification of therapeutic proteins, but its performance is influenced by a complex interplay of various parameters. Batch variations in ion exchange resins play a significant role in the robustness of downstream processing steps, with ligand density being a key factor affecting protein adsorption and separation performance. Using a model-based approach can help in understanding the impact of parameter variations, like ligand density, on the overall process.
JOURNAL OF SEPARATION SCIENCE
(2021)
Article
Engineering, Chemical
Yijian Li, Jianbo Hu, Jiyu Cui, Qingju Wang, Huabin Xing, Xili Cui
Summary: In this study, a novel porous material was developed for the adsorptive separation of acetylene/carbon dioxide mixtures. The material exhibited high dynamic breakthrough capacity for acetylene and low regeneration energy, thanks to its unique pore structure and intermolecular interactions.
FRONTIERS OF CHEMICAL SCIENCE AND ENGINEERING
(2022)
Article
Engineering, Environmental
Zhengyang Wang, Xiangyu Bi, Xiaoqing He, Yunchao Xie, Jian Lin, Baolin Deng
Summary: Decentralized and/or point-of-use systems are crucial for addressing challenging water quality issues. Sorption-based approaches, with their simplicity in operation, are ideal for such applications. This study presents a two-sorbent system consisting of Fe2O3-coated mesoporous carbon and nano-Fe2O3-coated activated carbon, which effectively removes arsenate through a capture-and-storage process with a short empty bed contact time.
Article
Engineering, Chemical
Sinuo Tan, Reinhard I. Boysen, Kei Saito, Milton T. W. Hearn
Summary: This study focuses on the dynamic adsorption behavior of proteins with thermoresponsive co-polymer grafted chromatographic materials. The dynamic binding capacity and rate of the ITA sorbent for bhLf were found to be greater at 50 degrees C than at 20 degrees C. Additionally, the electrostatic repulsion effects between negatively charged BSA and the modified surface of the ITA sorbent led to lower binding capacities compared to bhLF.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
C. Arab, R. El Kurdi, D. Patra
Summary: Zn(Cur)O NPs were synthesized and characterized, with adsorption increasing as the nanoparticle amount increases and dye concentration decreases. An acidic medium was found to improve the adsorption process.
MATERIALS TODAY CHEMISTRY
(2022)
Article
Instruments & Instrumentation
James W. Beattie, Ruth C. Rowland-Jones, Monika Farys, Hamish Bettany, David Hilton, Sergei G. Kazarian, Bernadette Byrne
Summary: Protein A affinity chromatography is an important step in isolating biotherapeutics with fragment crystallizable regions. The dynamic binding capacity (DBC) analysis assesses the binding of biotherapeutics to a protein A column. This study demonstrates the effectiveness of Raman spectroscopy in monitoring the DBC of protein A resins, providing additional quality control information about aggregation status and protein structure.
APPLIED SPECTROSCOPY
(2023)
Article
Engineering, Biomedical
Wenjuan Wang, Xiaoding Lin, Xiaoyan Dong, Yan Sun
Summary: This study developed a multifunctional theranostic nano-composite, HSA-BFP@CDs, for the diagnosis and treatment of Alzheimer's disease (AD). It demonstrated efficient detection of Aβ plaques and inhibition of Aβ aggregation, as well as scavenging of reactive oxygen species (ROS). The multifunctionality of HSA-BFP@CDs was further confirmed in an AD model, showing its potential for AD diagnosis and treatment.
ACTA BIOMATERIALIA
(2022)
Article
Engineering, Biomedical
Xiaoding Lin, Wei Liu, Xiaoyan Dong, Yan Sun
Summary: This study synthesized epigallocatechin gallate-derived carbonized polymer dots (E-CPDs) for the first time and demonstrated their multifunctional capability in inhibiting and disaggregating A,B fibrils, detecting amyloid plaques, and scavenging intracellular reactive oxygen species. The findings suggest that E-CPDs have great potential in the prevention and treatment of Alzheimer's disease (AD).
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Chemical
Yu Liu, Yu-Tong Li, Ling Liu, Shu Bai, Lin-Ling Yu, Yan Sun
Summary: Polyethylenimine (PEI)-grafted silica nanoparticles were used for electrostatic immobilization of a CO2 conversion enzyme. PEI served as a carbonic anhydrase mimic and assisted the enzyme cascade system for CO2 conversion. The immobilized enzyme showed enhanced stability, activity, reusability, and low cost, making PEI-grafted nanoparticles efficient nanocarriers for immobilizing CO2-converting enzymes in industrial applications.
CHEMICAL ENGINEERING & TECHNOLOGY
(2023)
Article
Engineering, Environmental
Yu Liu, Ling Liu, Zhi Qu, Linling Yu, Yan Sun
Summary: In this study, highly effective and non-cytotoxic laccase-like metallo-nanofibers were discovered, showing higher substrate affinity and catalytic efficiency than industrial laccase. These nanofibers exhibited stability, recyclability, and a wide substrate spectrum for the degradation of phenolic pollutants.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Chemistry, Physical
Jie Gao, Zhenfu Wang, Ranran Guo, Yang Hu, Xiaoyan Dong, Qinghong Shi, Yan Sun
Summary: A chemoenzymatic cascade system (GA&GOx@Au-SiO2) was developed for efficient production of gluconic acid from starch. The system utilized immobilized Au nanoparticles on mesoporous silica nanoparticles, along with co-immobilized glucoamylase and glucose oxidase. The proximity effect of these components facilitated rapid transport of reaction intermediates and timely removal of harmful H2O2, leading to improved gluconic acid yield and biocatalyst stability. The system demonstrated a gluconic acid yield of 97.8% within 30 minutes and retained 83.9% of initial activity after 10 cycles, outperforming the control systems without immobilized Au nanoparticles (57.5% initial activity). This chemoenzymatic cascade system offers a new strategy for enhancing enzymatic reactions involving H2O2 generation.
CATALYSIS SCIENCE & TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Dongjian Huang, Lin Zhang, Yan Sun
Summary: Polyethylene terephthalate (PET) is widely used due to its low cost and high durability, but the accumulation of waste PET has caused serious environmental pollution. Biodegradation of PET catalyzed by PET hydrolase (PETase) is more environmentally friendly, and the BbPETase(CD) from Burkholderiales bacterium is a promising enzyme for this purpose. In this study, the rational design of disulfide bridges in BbPETase(CD) was conducted to enhance its enzymatic performance. Computational algorithms were used to predict mutations, and the N364C/D418C variant with an additional disulfide bond showed improved expression, stability, and activity compared to the wild-type enzyme (WT). The variant also exhibited a significant increase in PET degradation.
Article
Chemistry, Multidisciplinary
Si Liu, Yan Sun
Summary: A facile and versatile polyelectrolyte-assisted encapsulation approach is presented, which enables the confinement of a cofactor-dependent multienzyme cascade within hydrogen-bonded organic frameworks. The system features excellent cargo loading, cascade activity recovery, and cofactor recycling capabilities.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemistry & Molecular Biology
Zhi Qu, Kun Chen, Lin Zhang, Yan Sun
Summary: This study introduces salt bridges into the flexible region of IsPETase to enhance its thermal stability. The designed variants showed higher melting points and more compact tertiary structures compared to the wild type, resulting in increased release of degradation products.
Article
Chemistry, Physical
Ziyi Yang, Zhenfu Wang, Xiaoyan Dong, Yan Sun
Summary: Gluconic acid is a valuable commodity in various industries, and the development of efficient and cost-effective production methods has become a research priority. Enzymatic synthesis has emerged as a promising approach for the production of gluconic acid due to its advantages.
Article
Biochemical Research Methods
Haotian Huang, Xiaoyan Dong, Yan Sun, Qinghong Shi
Summary: This study synthesized a biomimetic peptide ligand (bPL) SepFF-bPL for antibody purification and found that it effectively removed antibody aggregates but had poor removal of host cell proteins (HCPs) and DNA. To address this issue, chromatin extraction and anion exchange adsorption were introduced before SepFF-bPL chromatography for initial contaminant removal, reducing aggregation and loading burden. The purification train achieved high antibody recovery (68.7%) and low levels of HCPs (23 ppm) and DNA (below the limit of detection), meeting the requirements for clinical applications.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemistry & Molecular Biology
Yin Wu, Yan Sun
Summary: Mutations of surface positive-charged residues enhance the organic solvent resistance of DhaA, and the introduction of cationic polymers further improves its performance in organic solvents. Cationic polymers protect DhaA from damage, increase the stability of the enzyme, and enhance the substrate concentration.
Article
Engineering, Multidisciplinary
Bushu Peng, Shaoying Xu, Yue Liang, Xiaoyan Dong, Yan Sun
Summary: This study investigates the impact of phenol-soluble modulin (PSM) alpha 3, a protein secreted by Staphylococcus aureus, on the aggregation of amyloid beta-protein (A beta) related to Alzheimer's disease (AD). The results show that PSM alpha 3 monomer inhibits A beta aggregation and changes the aggregation pathway, while PSM alpha 3 oligomers promote A beta aggregation. Molecular dynamics simulations reveal the molecular interactions between A beta and PSM alpha 3 of different structures.
Article
Engineering, Environmental
Zixuan Li, Ziyi Yang, Qinghong Shi, Yan Sun
Summary: In this study, a defective metal-organic framework, dPCN-224, was synthesized and used for immobilizing laccase to effectively degrade BPA. The immobilized laccase showed improved performance in terms of activity recovery, stability, and removal efficiency compared to free laccase. The results demonstrate the potential of laccase@dPCN-224 for addressing environmental BPA pollutions.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2023)
Article
Engineering, Chemical
Renwen Tian, Yan Sun
Summary: The use of α-synuclein as a fusion chaperone resulted in improved enzymatic performance of PETase, with the fusion enzymes exhibiting higher activity and stability compared to PETase alone. The fusion proteins showed significantly enhanced PET degradation capability, possibly due to the binding affinity of the fused α-synuclein domain for PET.
CHINESE JOURNAL OF CHEMICAL ENGINEERING
(2023)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)