Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1478, Issue -, Pages 60-67Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2016.11.060
Keywords
Collagen crosslink analysis; Silica hydride column; Mass spectrometry; Mature crosslinks
Funding
- Leather and Shoe Research Association (LASRA(C)), Palmerston North, New Zealand through the New Zealand Ministry of Business and Innovation [LRX0801]
Ask authors/readers for more resources
We have developed a novel chromatographic analytical method for the simultaneous quantitation of collagen crosslinks. Seven non-derivatised crosslinks could be separated on a Cogent Diamond Hydride HPLC column using either isocratic or gradient conditions then detected by mass spectrometry. The total run time was less than 10 min which is significantly shorter than that previously reported. This is the first method in which histidinohydroxylysinonorleucine (HHL) and histidinohydroxymero-desmosine (HHMD) were separated and identified by mass spectrometry without the need for pre- or post-column derivatization. The CVs of the retention times of all seven crosslinks were less than 1% and the limit of detection (LOD) and the limits of quantitation (LOQ) were 0.07-0.13 pmol/mu L and 0.20-0.38 pmol/mu L, respectively. This novel method was used for the routine analysis and quantitation of crosslinks in different animal skins in which potential new collagen crosslinks were identified that are as yet undocumented. (C) 2016 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available