Article
Chemistry, Analytical
Elif Yazici, Suleyman Bodur, Sezin Erarpat, Ulas Emre Arslan, Sezgin Bakirdere
Summary: Acrylamide, a potentially neurotoxic and carcinogenic compound, is commonly found in environmental bodies due to industrial usage. This study compared different calibration strategies and proposed a highly accurate and precise method, LC-ID4-MS/MS, to quantify acrylamide in wastewater samples. The ID4 strategy showed superior accuracy and precision compared to EC and ISC methods.
MICROCHEMICAL JOURNAL
(2023)
Article
Biochemical Research Methods
Garnet McRae, Donald M. Leek, Juris Meija, Bryn Shurmer, Steven J. Lehotay, Joachim Polzer, Jeremy E. Melanson, Zoltan Mester
Summary: A freeze-dried bovine muscle-certified reference material (CRM), called BOTS-1, containing residues of commonly used veterinary drugs, was produced and certified. Liquid chromatography tandem mass spectrometry (LC-MS/MS) methods were used for value assignment, along with isotope dilution and standard addition approaches. Data from multiple organizations were used for value assignment. Results were obtained through an international inter-laboratory comparison. Primary standards were characterized using quantitative NMR (1H-qNMR). The certified mass fractions of the veterinary drug residues were reported with expanded uncertainties.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Medical Laboratory Technology
Shin Aleks, Connolly Shawn, Little Randie, Kabytaev Kuanysh
Summary: This study developed an assay for absolute quantitation of glycated albumin based on isotope dilution liquid chromatography-mass spectrometry. The method showed good analytical performance with a linear correlation between LC-MS/MS and Lucica method. This approach allows simultaneous quantitation of glycated and non-glycated peptides.
CLINICA CHIMICA ACTA
(2021)
Article
Medical Laboratory Technology
Jordi Farre-Segura, Neus Fabregat-Cabello, Chiara Calaprice, Laurent Nyssen, Stephanie Peeters, Caroline Le Goff, Etienne Cavalier
Summary: A rapid and specific LC-MS/MS method for glucagon quantitation has been developed and validated, showing stability and accuracy in comparison to other immunoassay methods. This method could potentially establish a standardized approach for glucagon analysis in routine care settings.
CLINICA CHIMICA ACTA
(2021)
Article
Biochemical Research Methods
Adel Achouba, Pierre Dumas, Pierre Ayotte
Summary: Ergothioneine and selenoneine are structurally related dietary antioxidants and cytoprotectants that may help prevent several chronic diseases associated with inflammation and aging. Researchers have developed a rapid, sensitive, specific, precise, and cost-effective analytical method to simultaneously quantify these compounds in human blood, and successfully applied it to volunteer blood samples.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Medical Laboratory Technology
Lizi Jin, Youli Lu, Xilian Yi, Meiwei Zhang, Jiangtao Zhang, Weiyan Zhou, Jie Zeng, Tianjiao Zhang, Chuanbao Zhang
Summary: This study compared the differences between different methods for measuring serum folate and found significant variations among the assays. Although there was good correlation between the assays, there was a lack of agreement. Therefore, it is important to routinely assess the accuracy and consistency of serum folate assays and promote standardization efforts.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2022)
Article
Biochemical Research Methods
Huu-Hien Huynh, Amandine Boeuf, Maxence Derbez-Morin, Anne-Marie Dupuy, Beatrice Lalere, Vincent Delatour, Joelle Vinh
Summary: An optimized method for quantifying PCT in human serum at sub-microgram per liter level was developed using LC-MS/MS, with linearity established through peptide-based calibration curves and accuracy evaluated using quality control materials. A correction factor was applied to compensate for digestion incompleteness and material loss, enabling accurate quantification with results traceable to SI units. The method showed a twofold lower PCT concentration compared to immunoassay results when applied to serum samples from sepsis patients.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Ashley Beasley-Green, N. Alan Heckert
Summary: The emergence of mass spectrometry (MS)-based methods for protein quantification in clinical applications has necessitated the need for accurate and consistent measurements. A comprehensive approach for estimating the measurement uncertainty of a MS-based procedure for protein quantification is outlined using a bottom-up approach. The uncertainty components of the procedure are evaluated using a cause-and-effect diagram, and statistical equations are derived to determine the overall combined uncertainty.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Carlos Goncalves, Adam Tolgyesi, Katrien Bouten, Fernando Cordeiro, Joerg Stroka
Summary: This paper describes new technical features that improved a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the analysis of Alternaria toxins (ATs) in tomato, wheat and sunflower seeds. The improvements include the use of isotopically labelled internal standards, fine-tuning of sample extraction and solid-phase extraction (SPE), and the use of a more suitable analytical column. The method shows adequate precision and trueness, and has been recognized by the European Committee for Standardization.
Article
Biochemical Research Methods
Shimin Chen, Charles Yang, Melanie Downs
Summary: This study developed a mass spectrometry-based method to quantify commercially processed soy ingredients in food, demonstrating its significance in detecting and quantifying soy allergens.
JOURNAL OF PROTEOMICS
(2021)
Article
Medical Laboratory Technology
Anett Engel, Lena Ruhe, Neeraj Singh, Jo Anne Wright, Franziska Liesch, Friederike Bauland, Annika I. I. Ostermann, Tamara Sumalowitsch, Vincent J. T. Schweinsberg, Andrea Geistanger, Johannes Kolja Hegel, Christian Geletneky, Judith Taibon
Summary: An isotope dilution-liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS)-based candidate reference measurement procedure (RMP) was developed for quantification of methotrexate in human serum and plasma. The absolute content of methotrexate in the standard was determined using quantitative nuclear magnetic resonance (qNMR). The method showed good performance in terms of selectivity, specificity, precision, and accuracy.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Food Science & Technology
Wannes Hugo R. Van Hassel, Mirjana Andjelkovic, Benoit Durieu, Viviana Almanza Marroquin, Julien Masquelier, Bart Huybrechts, Annick Wilmotte
Summary: Belgium currently monitors toxic cyanobacterial blooms through regional environmental agencies, but protocols and locations are inconsistent. This study analyzed water samples from five lakes in Wallonia, as well as occasional blooms in Flanders and Brussels. Microcystin concentrations were detected using LC-MS/MS, while the presence of the mcyE gene and dominant cyanobacterial species were determined through PCR and DNA sequencing. The results showed that most samples exceeded the recommended guidelines for microcystin concentrations, and a diverse range of cyanobacterial species were identified. The mcyE gene was present in the majority of samples.
Article
Chemistry, Analytical
Viktoria Lindemann, Jessica Schmidt, Benedikt Cramer, Hans-Ulrich Humpf
Summary: The analysis of trace contaminants in environmental samples is crucial for exposure assessment and evaluation of potential risks to human health. Currently, high resolution mass spectrometry methods are gaining popularity due to their enhanced selectivity, although the complex composition of environmental samples can have significant effects on the performance of mass analyzers.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Feng Yang, Yi Li, Hong Pan, Kaili Wu, Yuanfu Lu, Fuguo Shi
Summary: DOPAL, a toxic metabolite of dopamine, causes neurodegeneration by covalent binding with proteins; a sensitive UPLC-MS/MS method was developed for quantification of DOPAL in rat brain tissue samples; significant increase of DOPAL level was found in the striatum of rats in a 6-OHDA-induced Parkinson's disease model.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Chemistry, Medicinal
E. Sook Yen, Yunli Hu, Rosalynn Molden, Haibo Qiu, Ning Li
Summary: Monitoring and quantifying low abundance host cell proteins (HCPs) in therapeutic proteins is crucial for ensuring product quality and safety. A practical and effective strategy for identifying and quantifying problematic HCPs at low levels has been developed, facilitating downstream purification process improvement. This workflow can be easily adapted to detect and quantify other problematic HCPs at extremely low levels in therapeutic protein drug candidates.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)
Article
Chemistry, Analytical
Koyo Ido, Tomoko Ariga, Yanbei Zhu, Akiko Hokura, Kazumi Inagaki
Editorial Material
Instruments & Instrumentation
Lianhua Dong, Zhiwei Sui, Jing Wang, Vincent Tang, Winnie Chum, Foo-wing Lee, Della Sin, Melina Perez-Urquiza, Malcolm Burns, Mojca Milavec, Chaiwat Prawettongsopon, Kate R. Griffiths, Jacob L. H. McLaughlin, Sachie Shibayama, Sema Akyurek, Muslum Akgoz
Article
Instruments & Instrumentation
M. Sargent, H. Goenaga-Infante, K. Inagaki, L. Ma, J. Meija, A. Pramann, O. Rienitz, R. Sturgeon, J. Vogl, J. Wang, L. Yang
Article
Environmental Sciences
Keisuke Nakamura, Takamitsu Otake, Nobuyasu Hanari, Akiko Takatsu
JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES
(2019)
Article
Biochemical Research Methods
Sachie Shibayama, Shin-ichiro Fujii, Kazumi Inagaki, Taichi Yamazaki, Mariko Yoshioka, Satoko Matsukura, Akira Sasaki, Tetsushi Suyama, Naohiro Noda, Yuji Sekiguchi, Akiko Takatsu
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2019)
Article
Biochemical Research Methods
Thi Thanh Huong Tran, Juok Kim, Nordiana Rosli, Inkyu Mok, Kyong Hwa Oh, Hwashim Lee, Seon-Pyo Hong, You-Xun Jin, Liqing Wu, Jing Wang, Yohei Sakaguchi, Tomoya Kinumi, Akiko Takatsu, Sook-Kyung Kim, Ji-Seon Jeong
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2019)
Article
Chemistry, Applied
Takashi Yarita, Shinsuke Inagaki, Ayano Miyamoto, Taichi Yamazaki, Migaku Kawaguchi, Hajime Uchida, Akiko Takatsu, Toshiyuki Suzuki
Editorial Material
Instruments & Instrumentation
Zoltan Mester, Philippe Corbisier, Stephen L. R. Ellison, Yunhua Gao, Chunyan Niu, Vincent Tang, Foo-Wing Lee, Melina Perez-Urquiza, Angel Ramirez Suarez, Malcolm Burns, Mojca Milavec, Kanjana Wiangnon, Kate R. Griffiths, Jacob L. H. McLaughlin, Sachie Shibayama, Akiko Takatsu, Muslum Akgoz, Maxim Vonsky, Andrei Runov, John Emerson Leguizamon Guerrero
Article
Chemistry, Analytical
Koyo Ido, Rina Matsushita, Shin-ichiro Fujii, Shin-ichi Miyashita, Tomonari Umemura, Akiko Hokura, Kazumi Inagaki
ANALYTICAL SCIENCES
(2020)
Article
Biochemical Research Methods
Takema Hasegawa, Sachie Shibayama, Yukiko Osumi, Hiroshi Sentsui, Megumi Kato
Summary: A single-molecule assay using digital ELISA has been developed for ultra-sensitive virus detection. This study evaluated the linearity and sensitivity of digital ELISA and established a measurement system for detecting recombinant viral proteins and influenza viruses. The system showed high specificity and robustness, with a low limit of detection for both recombinant viral proteins and viruses.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Tomoko Ariga, Tomohiro Narukawa, Yanbei Zhu, Shin-ichi Miyashita, Izumi Kudo, Masae Kocuchi, Kazumi Inagaki
Article
Chemistry, Analytical
Tomoko Ariga, Yanbei Zhu, Kazumi Inagaki
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
(2019)
Article
Chemistry, Analytical
Fang-hsin Lin, Shin-ichi Miyashita, Kazumi Inagaki, Yi-Hung Liu, I-Hsiang Hsu
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
(2019)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)