Article
Chemistry, Multidisciplinary
De-Fa Hou, Meng-Lei Li, Cong Yan, Ling Zhou, Zheng-Ying Liu, Wei Yang, Ming-Bo Yang
Summary: A sustainable esterification strategy was developed to synthesize cellulose oleate (COE), which can be processed into homogeneous films with flexibility, transparency, and hydrophobicity. This strategy offers advantages in terms of reduced reactant dosage, improved sustainability, and enhanced efficiency, providing a potential green route for industry manufacturing of melt-processable cellulose esters.
Article
Engineering, Chemical
Moritz Hofer, Michael Grube, Christine Friederike Burmeister, Peter Michalowski, Sabrina Zellmer, Arno Kwade
Summary: Mechanochemical syntheses are potential environmentally friendly and scalable processes. In this study, the solid electrolyte Li3PS4 was successfully synthesized in less than 5 hours by systematically varying and optimizing the process parameters in a high energy ball mill. The synthesized electrolyte samples were characterized for their composition, morphology, particle size distribution, and ionic conductivity. The results provide a better understanding of the process-structure-property relations and process efficiency.
ADVANCED POWDER TECHNOLOGY
(2023)
Article
Food Science & Technology
Linhong Fan, Wenxiang Fan, Yuqi Mei, Longchan Liu, Linnan Li, Zhengtao Wang, Li Yang
Summary: Mechanochemical assisted extraction (MCAE) is an efficient, economic, and environmentally-friendly method for extracting bioactive components from complex matrices. It uses high energy mechanical force to separate target components from natural products in aqueous media without the need for harmful organic solvents. MCAE offers a quick and reproducible process with a direct transfer of solutions for subsequent analysis.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2022)
Article
Agricultural Engineering
Si-Chen Zhu, Min-Zhen Shi, Ya-Ling Yu, Jun Cao
Summary: The novel IS-CPR-IL-MCE technique coupled with ultrahigh performance liquid chromatography showed efficient extraction, concentration, and separation of five alkaloids from lotus leaf. The method's parameters were optimized to achieve the highest extraction yield, and it exhibited good linearity, low detection limits, acceptable reproducibility, and satisfactory recovery. The results demonstrated successful application of the proposed method for alkaloid extraction from lotus leaf.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Biochemical Research Methods
Masato Taoka, Kota Kamei, Akiko Kashima, Yuko Nobe, Takahiro Takekiyo, Takamasa Uekita, Tohru Ichimura
Summary: Many ion channels and receptor proteins are potential targets for new drugs. This study presents a novel proteomic sample preparation platform that allows for the processing of sub-microgram level starting materials using an ionic liquid-based sample preparation strategy and an in-StageTip technique. This approach enables the rapid solubilization and analysis of integral membrane proteins from human samples, surpassing the performance of conventional methods.
ANALYTICAL BIOCHEMISTRY
(2023)
Article
Food Science & Technology
Lei Liu, Jun Xi
Summary: Mechanochemical-assisted extraction (MCAE) combined with surfactant instead of alkaline grinding medium can increase the yield of watermelon seeds protein (WSP) under optimal conditions and retain better protein quality. Compared with traditional alkaline extraction, MCAE with surfactant extraction method can more effectively prevent protein denaturation.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Si-Chen Zhu, Ya-Ling Yu, Min-Zhen Shi, Yan Chen, Jun Cao
Summary: In this study, an ionic liquid vesicle-based mechanochemical-assisted extraction (MCAE) method was proposed for the first time and successfully applied for the extraction of weak acid compounds from Mori Fructus. The factors affecting the extraction efficiency were systematically evaluated, and the optimal conditions were determined. Scanning electron microscopy and transmission electron microscopy were used to verify the formation of vesicles and identify the changes in physicochemical properties during the extraction process.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2022)
Article
Chemistry, Analytical
Joshua J. Coon, Laura K. Muehlbauer, Annie Jen, Yunyun Zhu, Yuchen He, Evgenia Shishkova, Katherine A. Overmyer
Summary: Multi-omics analysis is a powerful and increasingly used method to understand complex biological systems. However, the sample preparation process for multi-omics is time-consuming and wasteful. We propose a simple and efficient method, called BAMM, which saves time and achieves comparable results to existing methods.
ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Colin F. Poole
Summary: High-performance thin-layer chromatography is a promising technique for high-throughput separations. The sample preparation is crucial to control matrix interferences and improve the detectability of target compounds. Recent trends have shifted towards bioassays and mass spectrometry. Traditional methods are being replaced by automated instrumental approaches for faster and streamlined sample processing. Liquid-liquid extraction is commonly used, although solid-phase extraction is gaining popularity. Planar solid-phase extraction is increasingly used for complex samples. Factors driving current interest include faster and simpler sample workflows, analysis of more complex samples, and the determination of minor contaminants with high concentration factors.
JOURNAL OF SEPARATION SCIENCE
(2023)
Review
Food Science & Technology
Adrian Fuente-Ballesteros, Patricia Brugnerotto, Vinh Dinh Nguyen, Ana C. O. Costa, Jose Bernal, Ana M. Ares
Summary: Pesticide residues in royal jelly, a valuable bee product, have received less attention compared to other bee products. The contamination can occur when bees collect pollen or nectar from pesticide-treated flowers or due to inappropriate use of pesticides in beehive treatments. It is crucial to develop effective sample preparation methods for extracting pesticides from royal jelly for subsequent analysis.
Article
Chemistry, Multidisciplinary
Francisco Pena-Pereira, Isela Lavilla, Carlos Bendicho
Summary: This review examines cutting-edge advances in greener analytical methods for sample preparation, focusing on the use of environmentally friendly solvents and adsorbents, integration of unitary steps, miniaturization, and automation. The paper also addresses concluding remarks and additional considerations for further improvements.
CURRENT OPINION IN GREEN AND SUSTAINABLE CHEMISTRY
(2021)
Review
Chemistry, Analytical
Melisa Rodas, Katerina Fikarova, Finnian Pasanen, Burkhard Horstkotte, Fernando Maya
Summary: Zeolitic imidazolate frameworks, with high porosity and chemical stability, have been used to enhance sensitivity and selectivity in analytical methods. In addition to direct use in sample preparation protocols, various strategies for magnetization or incorporation into sorbent particles, monoliths, fibers, stir bars, or thin films have been developed.
JOURNAL OF SEPARATION SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Gabor Bati, Shoba Laxmi, Mihaiela C. Stuparu
Summary: Corannulene, a curved polycyclic aromatic hydrocarbon, has been synthesized on a multigram scale using mechanochemical synthesis. While a mixer mill approach was suitable for gram-scale synthesis, larger scales utilized planetary mills. The yields were highest at a rotation rate of 400 rpm, with an isolated yield of 90% for 15g of corannulene obtained in a single milling cycle. In total, 98g of corannulene was produced using ball milling-based grinding techniques, highlighting the potential for mechanochemistry in the synthesis of highly curved nanocarbons.
Article
Agriculture, Multidisciplinary
Lei Zheng, XingYi Zhu, Jialu Wang, Weike Su
Summary: The study developed mechanochemical-assisted extraction (MCAE) and mechanochemical-assisted enzyme catalysis (MCAEC) methods for extracting and enzymatic hydrolysis of calcium phytate from defatted rice bran, respectively. The optimized MCAE conditions resulted in a calcium phytate yield of 97.0 mg/g, while the optimized MCAEC conditions achieved a hydrolysis rate of 39.7% for calcium phytate. Compared to traditional methods, MCAE and MCAEC demonstrated higher yields and hydrolysis rates, respectively.
CHEMICAL AND BIOLOGICAL TECHNOLOGIES IN AGRICULTURE
(2023)
Review
Chemistry, Analytical
Belen Herce-Sesa, Jose A. Lopez-Lopez, Carlos Moreno
Summary: The incorporation of ionic liquids in LPME for metal extraction has shown great potential in sample preparation for environmental analysis. This has led to the development of more efficient and selective extraction methods, with the use of task specific ionic liquids and deep eutectic solvents. The future development of IL-LPME for metal extraction shows promise for further advancements in the field.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Applied
Noorfatimah Yahaya, Zi-Ao Huang, Binjun Yan, David D. Y. Chen
Summary: The method presented in this study allows for the sensitive determination of bisphenol A and its analogues in bottled tea beverages at the ng/mL level by utilizing dynamic pH junction. The optimized analyte focusing results in enhanced signal detection, with good linearity, low detection limits, high analyte recovery, and promising practical applications.
Article
Chemistry, Multidisciplinary
Dan Cao, Min Liu, Yung-Chih Su, Zehui Yang, Wentao Bi, David Da Yong Chen
Summary: This study investigates the mechanism and influencing factors of dyeing polyester fabrics (PETFs) using deep eutectic solvents (DESs). The research finds that anthraquinone dyes have different interactions with DESs, and successful dyeing of PETF depends on strengthening the interactions between PETF and the dyes. The type of DES, hydrogen bond acceptor-hydrogen bond donor ratio, dye concentration, dyeing temperature, and dyeing time also affect the adsorption and dyeing efficiency.
SUSTAINABLE CHEMISTRY AND PHARMACY
(2022)
Article
Biochemical Research Methods
Rongfan Ren, Hongli Li, Qing Jiang, Xing Wang, David Da Yong Chen
Summary: Characterization of structural isomers of bioactive molecules is important but challenging due to their similar structures. This study demonstrated a simple and robust method using in situ methylation reaction with TMAH and DART ion source to characterize saponin isomers. The method allowed for the identification of ginsenosides in Panax ginseng without the need for chromatographic separation or offline sample pretreatment.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Min Liu, Simin Wang, Wuxia Ge, Wentao Bi, David Da Yong Chen
Summary: This study systematically investigated the influence of host-guest interactions on the analytical performance of direct analysis in real time mass spectrometry (DART-MS) by studying the interactions between cyclodextrins (CDs) and Sudan dyes. The results showed that the host-guest interaction between CDs and Sudan dyes affected the quantitative analysis of the target compounds by lowering the signal intensity. Furthermore, the addition of organic solvents weakened the host-guest interaction and improved the signal intensity in DART-MS, particularly in solid-phase microextraction (SPME).
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Rongfan Ren, Minghui Yuan, Hongli Li, David Da Yong Chen
Summary: Carbohydrates are essential for energy supply and biological functions, but identifying and distinguishing their isomers has been challenging due to their similar structures and possible glycosidic linkages. This study developed an ionization tandem mass spectrometry method to characterize disaccharide isomers by in situ methylation. The method allowed for immediate methylation of hydroxyl groups on disaccharides, enabling their ionization and distinction through mass spectrometry. This approach was used to directly identify disaccharide isomers in real commercial products without complex pretreatment or chromatographic separation.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Applied
Min Liu, Simin Wang, Wentao Bi, David Da Yong Chen
Summary: Deep eutectic systems (DESys) formed by hydrogen bond acceptor (HBA) and polysaccharide (hydrogen bond donor, HBD) in water can enhance the extraction efficiency and retain the physicochemical properties of polysaccharides. This method simplifies the recovery and purification process of polysaccharides.
Article
Engineering, Environmental
Zhaoyang Wang, Tianhao Li, Wentao Bi, Wenling Zhang
Summary: In this study, magnetorheological fluids (MRFs) with a choline chloride/glycerol deep eutectic solvent (DES) as the carrier liquid were developed and studied. The DES showed strong hydrogen bonding interactions with the functional groups on the surfaces of the magnetic particles, leading to improved mechanical properties and sedimentation stability of the MRF. Factors such as the type and water content of DES, the HBA:HBD ratio, and the concentration of magnetic particles were found to significantly influence the magnetorheological properties. The research demonstrates the design and fabrication of a new type of eco-friendly, high-performance MRF for future development of efficient MRFs based on chemical or molecular interactions.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Biochemical Research Methods
Yuanli Dai, Chang Sun, Yunhe Yang, Shuanghao Wang, Huihui Li, David Da Yong Chen
Summary: The thermodynamic properties of molecular recognition in host-guest inclusion complexes were studied using Taylor dispersion analysis (TDA). TDA provided rapid and convergent results for the binding properties of cyclodextrins (CDs) and their derivatives with physiologically active substances. The TDA results were compared with those obtained by nuclear magnetic resonance and affinity capillary electrophoresis (ACE), showing slightly lower binding constants from ACE.
Article
Chemistry, Applied
Zhixin Xiao, Min Liu, Wentao Bi, David Da Yong Chen
Summary: In-depth studies of extraction mechanism using deep eutectic solvents (DES) and ionic liquids (IL) reveal similarities between the two systems. New applications of ILs and DES in the extraction of nutritional natural products are presented. The study systematically investigates the extraction behavior of ChCl and [HMIm][Cl] in DES and IL, respectively, for target compound extraction from Moringa oleifera leaves. The results show that both extraction methods are based on the formation of a DESys, which can be improved by the formation of a new IL deep eutectic system based on hydrogen bond interaction.
Article
Chemistry, Analytical
Xin Geng, Kai Zhang, Hongli Li, David Da Yong Chen
Summary: A robust ambient ionization mass spectrometry method using a modified direct analysis in real time (DART) ion source was developed for online analysis of breath volatiles. The method is fast, simple, versatile, and has the potential to evaluate the true state of human exhaled breath.
Article
Chemistry, Analytical
Binjun Yan, Mengtian Shi, Siyu Cai, Yuan Su, Renhui Chen, Chiyuan Huang, David Da Yong Chen
Summary: Proteomics is essential for understanding biology and diseases, with liquid chromatography-tandem mass spectrometry (LC-MS/MS) being a widely used platform. Data-independent acquisition (DIA) improves the reproducibility of LC-MS/MS in proteomics research. However, existing DIA data processing tools sometimes lead to significant deviations in quantification. This study presents a cross-run ion selection and peak-picking (CRISP) tool that takes advantage of the consistency of DIA data across runs to filter out interfering signals, resulting in improved quantification accuracy.
ANALYTICAL CHEMISTRY
(2023)
Article
Green & Sustainable Science & Technology
Simin Wang, Min Liu, Wuxia Ge, Can Jin, Wentao Bi
Summary: This study explores the efficient extraction of lignin from wood meal using a deep eutectic system (DESys) and characterizes the obtained lignin. Various factors such as DESys components, extraction time, temperature, water content, and solid-liquid ratio were investigated for their impact on the lignin extraction process. The study also examined the utilization of the residue after lignin extraction for the adsorption of cationic pollutants. The results showed that DESys-based extraction yielded lignin with similar properties to traditionally extracted lignin and the residue effectively adsorbed cationic dye pollutants.
JOURNAL OF CLEANER PRODUCTION
(2023)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
