Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1443, Issue -, Pages 111-117Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2016.03.026
Keywords
Freeze casting method; Aligned macropores; Monolith; Chromatography
Funding
- Natural Science Foundation of China [21206097, 21476144]
- Science Foundation for The Excellent Youth Scholar of Sichuan University [2014SCU04A04]
- Innovative pilot project of Sichuan province [201510610524]
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In the present study, a freeze casting method combined with particle accumulation was applied to fabricate the aligned macroporous monolith for high-performance protein chromatography. For the preparation, the reactive colloids were first prepared by using glycidyl methacrylate and ethylene glycol dimethacrylate as monomers. Subsequently, these colloids accumulated regularly and polymerized into the aligned macroporous monolith. The aligned porous structure of the monolith was characterized by SEM, mercury intrusion, and flow hydrodynamics. The results revealed that the generated monolith was possessed of aligned macropores in size of about 10 pm and high column permeability. Finally, after being modified with sulfonated groups, the monolith was evaluated for its chromatographic performance. It demonstrated that the aligned macropores endowed the monolith with excellent adsorption capacity and high column efficiency. (C) 2016 Elsevier B.V. All rights reserved.
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