Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1440, Issue -, Pages 260-265Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2016.02.055
Keywords
Prostaglandin; Eicosanoid; LC-MS/MS; Unispray; Electrospray
Funding
- ARIADME, a European FP7 ITN Community's Seventh Framework Programme [607517]
- Ed Sprake (Waters)
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Eicosanoids, including prostaglandins and thromboxanes are lipid mediators synthetized from polyunsaturated fatty acids. They play an important role in cell signaling and are often reported as inflammatory markers. LC-MS/MS is the technique of choice for the analysis of these compounds, often in combination with advanced sample preparation techniques. Here we report a head to head comparison between an electrospray ionization source (ESI) and a new atmospheric pressure ionization source (UniSpray). The performance of both interfaces was evaluated in various matrices such as human plasma, pig colon and mouse colon. The UniSpray source shows an increase in method sensitivity up to a factor 5. Equivalent to better linearity and repeatability on various matrices as well as an increase in signal intensity were observed in comparison to ESI. (C) 2016 Elsevier B.V. All rights reserved.
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