Article
Chemistry, Applied
Rui Zhang, Xiaosong Zhai, Wentao Wang, Hanxue Hou
Summary: This study investigated the effects of different emulsifiers on the structure and properties of agar/maltodextrin-beeswax films. Emulsifiers with lower hydrophilic-lipophilic balance values were found to improve the distribution and compatibility of beeswax in the film matrix. The incorporation of different emulsifiers had diverse impacts on the film properties, with agar/maltodextrin-beeswax-polysorbate 80 film showing the highest stiffness and agar/maltodextrin-beeswax-glycerol monostearate film exhibiting the best physicochemical properties.
Article
Biochemistry & Molecular Biology
M. Pilar Vazquez-Tato, Julio A. Seijas, Francisco Meijide, Francisco Fraga, Santiago de Frutos, Javier Miragaya, Juan Ventura Trillo, Aida Jover, Victor H. Soto, Jose Vazquez Tato
Summary: The lipophilicity of 15 derivatives of sodium cholate was theoretically determined using the Virtual log P method. The relationship between the octan-1-ol/water partition coefficient and the critical micelle concentration (cmc) was established through a double-logarithm relationship. The study also investigated the demicellization of aggregates formed by sodium cholate derivatives with bulky hydrophobic substituents.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Carlos A. Valdez, Roald N. Leif
Summary: The field of gas chromatography-mass spectrometry (GC-MS) in the analysis of chemical warfare agents, particularly focusing on organophosphorus-based nerve agents, is constantly evolving and essential for the rapid and accurate detection of these lethal agents. Derivatization reactions, primarily silylations and alkylations, have played a crucial role in efficiently detecting products arising from the degradation of these nerve agents for retrospective identification.
Article
Biochemical Research Methods
Fotouh R. Mansour, Emily F. Hilder, Faisal K. Algethami, Taghrid Alomar, R. Dario Arrua
Summary: The study investigated the effect of different chain lengths of polyoxyethylene ethers on the microporogenic properties of Brij surfactants. The objective was to understand the role of each porogen and establish criteria for selecting non-ionic surfactants based on hydrophilic/lipophilic balance (HLB) values. The results showed that increasing the HLB led to higher monolith density and surface areas. The optimal HLB range was found to be 10 to 15. Lower HLB surfactants formed nonporous or less efficient columns, while higher HLB surfactants formed non-permeable columns. Adjusting the HLB was possible by mixing surfactants of different HLB. The prepared monoliths exhibited successful separations of sulfa drugs, nonsteroidal anti-inflammatory drugs, and benzoic acid derivatives, meeting the desired porosity, surface area, morphology, and chromatographic separation requirements.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Review
Chemistry, Analytical
Adriana Leandra Santoro, Pedro H. Vendramini, Deisi L. Braga, Lucas Gelain Martins, Gabriel F. Dos Santos, Gesiane S. Lima, Ljubica Tasic, Marcelo A. Mori
Summary: This review presents the main steps involved in C. elegans lipidomics and metabolomics analyses, discussing the advantages and drawbacks of different methods and listing commonly used protocols and applications.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Mai Otsuka, Akinori Yamaguchi, Hajime Miyaguchi
Summary: A new ion chromatography-tandem mass spectrometry method was developed for the analysis of nerve agent degradation products in human urine. This method successfully detected six conventional nerve agent degradation products and six Novichok agent degradation products, despite their differences in hydrophilicity and acidity. By using an ammonium regeneration solution, the peak shapes were greatly improved compared to the results obtained with the ordinary IC-MS/MS configuration. The method also demonstrated low detection limits and acceptable linearity.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Review
Food Science & Technology
Meng-Lei Xu, Yu Gao, Xiao Wang, Xiao Xia Han, Bing Zhao
Summary: Food safety and quality have become a major concern, with GC-MS/MS being preferred in food safety laboratories for its sensitivity. Multi-target analysis and efficient purification methods are key trends in food safety analysis.
Article
Biochemistry & Molecular Biology
Jin Young Lee, Kyoung Chan Lim, Hyun Suk Kim
Summary: In this study, the degradation products and fragmentation pathways of Novichok (A234) in aqueous solution at different pH levels were investigated using liquid chromatography-tandem mass spectrometry. The results showed that A234 had the fastest hydrolysis rate under acidic conditions, with the same degradation products formed at all pH levels.
Article
Environmental Sciences
Raluca Elena Ginghina, Adriana Elena Bratu, Gabriela Toader, Andreea Elena Moldovan, Tudor Viorel Tiganescu, Ramona Elena Oncioiu, Panaghia Deliu, Razvan Petre, Gabriel Epure, Munizer Purica
Summary: This study extensively investigated the efficiency of decontamination of sulfur mustard (HD) and soman (GD) using three types of reactive organic suspensions with nanosized adsorbents (ZnO, TiO2, and zeolite). The decontamination solutions were assessed through GC-MS analysis to determine the degradation of the toxic agents over time intervals, while also monitoring the conversion of the chemical warfare agents into decontamination products over a period of 24 hours.
Article
Biochemical Research Methods
Jonathan Bilko, Yiwei Deng
Summary: A method using innovative sample preparation was developed for determination of cocaine on banknotes. A sonication-enhanced technique was used for aqueous extraction of cocaine. Quantitation of cocaine was achieved using HPLC with UV detection, and identification was accomplished using GC-MS. Multiple calibration techniques were incorporated to simultaneously determine cocaine contents and assess matrix effects. Statistical analysis confirmed that matrix effects were not significant with the sample preparation employed. The method is simple and suitable for drug analysis and forensic science applications.
DRUG TESTING AND ANALYSIS
(2022)
Review
Chemistry, Analytical
Khaja Moinuddin Shaik, Bhaskar Sarmah, Gaurav Suresh Wadekar, Pramod Kumar
Summary: This review discusses the recent detection of nitrosamine impurities in pharmaceutical products, including various detection methods, mechanisms of action, sample preparation techniques, and regulatory limits.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Woo-Hyeon Jeong, Jin-Young Lee, Kyoung-Chan Lim, Hyun-Suk Kim
Summary: Nonapeptide adducts containing the active site serine inhibited by ethyl or methyl phosphoramidofluoridate were studied using LC-MS/HRMS, revealing expected biomarkers with a higher abundance of doubly charged ions for trace analysis.
Review
Food Science & Technology
Pau Lian Peng, Lee Hoon Lim
Summary: This literature review provides an overview of polycyclic aromatic hydrocarbons (PAHs), including their background, sources of exposure, occurrence in food and environment, and health hazards. It also presents analytical methods used for the analysis of PAHs in tea, coffee, milk, and alcoholic samples over the past 16 years.
FOOD ANALYTICAL METHODS
(2022)
Article
Toxicology
Mai Otsuka, Akinori Yamaguchi, Hajime Miyaguchi
Summary: In this study, a HILIC-MS/MS method was developed to detect Novichok agent degradation products in human urine. With a simple pretreatment method, the six degradation products of Novichok agents were successfully separated and detected with high sensitivity.
FORENSIC TOXICOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Van-An Duong, Hookeun Lee
Summary: Liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based proteomics is a powerful technique for analyzing cellular, tissue, and fluid proteomes. While LC-MS/MS and data analysis techniques have been well-developed, sample preparation remains a challenging and laborious process. In this review, various methods for sample preparation in proteomics, as well as devices and methods for integrating different steps of sample preparation and peptide fractionation, are outlined and discussed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Analytical
Marcos Tascon, Varoon Singh, Mohammad Huq, Janusz Pawliszyn
ANALYTICAL CHEMISTRY
(2019)
Article
Agriculture, Multidisciplinary
Abir Khaled, Varoon Singh, Janusz Pawliszyn
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2019)
Review
Chemistry, Analytical
Chiranjit Ghosh, Varoon Singh, Jonathan Grandy, Janusz Pawliszyn
JOURNAL OF SEPARATION SCIENCE
(2020)
Article
Chemistry, Analytical
Diana M. Cardenas-Soraca, Varoon Singh, Emir Nazdrajic, Tijana Vasiljevic, Jonathan J. Grandy, Janusz Pawliszyn
Article
Chemistry, Analytical
Jonathan J. Grandy, Virginia Galpin, Varoon Singh, Janusz Pawliszyn
ANALYTICAL CHEMISTRY
(2020)
Article
Engineering, Environmental
Daniel A. Rickert, Varoon Singh, Milaan Thirukumaran, Jonathan J. Grandy, Joao Raul Belinato, Maryam Lashgari, Janusz Pawliszyn
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2020)
Article
Chemistry, Applied
Dominika Gruszecka, Jonathan Grandy, Emanuela Gionfriddo, Varoon Singh, Janusz Pawliszyn
Summary: A novel method using TF-SPME was developed to test for PCAs in commercial cod liver oil samples. The method showed effective extraction of contaminants from oily matrices and provided promising results in detecting specific pollutants in fish oil brands.
Article
Biochemical Research Methods
Christian Ieritano, Daniel Rickert, Joshua Featherstone, John F. Honek, J. Larry Campbell, J. C. Yves Le Blanc, Bradley B. Schneider, W. Scott Hopkins
Summary: The study demonstrates that adding solvent vapor to DMS can stabilize protonated peptides, reduce collision-induced fragmentation, and lower temperatures. Additionally, the solvation propensity of solvents aids in stabilizing charge density.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Chemistry, Analytical
Milaan Thirukumaran, Varoon Singh, Yohei Arao, Yuka Fujito, Masayuki Nishimura, Tairo Ogura, Janusz Pawliszyn
Summary: Probe electrospray ionization (PESI) has been successfully integrated with solid-phase microextraction for highly sensitive quantitation of drugs of abuse. The optimal desorption solvent for SPME-PESI-MS/MS was determined, and a method for quantifying drugs of abuse in small volumes of plasma without matrix modification was developed. Intra-day accuracy for eight drugs of abuse was within acceptable ranges, with some exceptions noted for specific compounds.
Article
Chemistry, Analytical
Liqin Chen, Varoon Singh, Daniel Rickert, Abir Khaled, Janusz Pawliszyn
Summary: The study developed two pretreatment methods, TF-SPME and PFSPE, for the analysis of biogenic monoamines in urine. Results showed that the TF-SPME method outperformed the PFSPE method in terms of extraction efficiency, matrix effects, and linear range, ensuring higher accuracy of simultaneous detection of all compounds of interest.
Article
Chemistry, Analytical
Yu Jing, Varoon Singh, Liqin Chen, Janusz Pawliszyn
Summary: The study introduces a high-throughput method for the simultaneous detection of four OPFR metabolites in urine samples, which is reliable, accurate, and compliant with the guidelines of the Food and Drug Administration.
Article
Chemistry, Multidisciplinary
Khaled Murtada, Virginia Galpin, Jonathan J. Grandy, Varoon Singh, Freddy Sanchez, Janusz Pawliszyn
Summary: A Car/PDMS based TF-SPME method was developed for identifying VOCs in hot tub air and human breath samples. Membranes with a 5% particle ratio were found to be optimal for extraction efficiency, with Car/PDMS coating capturing the highest amounts of target compounds. Both Car/PDMS and NCar/PDMS showed good repeatability for all analytes tested, making them suitable for various applications.
SUSTAINABLE CHEMISTRY AND PHARMACY
(2021)
Article
Biochemical Research Methods
Daniel Rickert, German Augusto Gomez-Rios, Varoon Singh, Janusz Pawliszyn
Summary: This study evaluated the effect of spatial positioning of coated-blade spray (CBS) devices with respect to the mass spectrometry (MS) inlet when coupling to different MS platforms. The results showed that optimizing the MS parameters, such as the position of the CBS tip regarding the MS inlet, is vital to exploit the full quantitative potential of this technology.
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2022)
Article
Multidisciplinary Sciences
Margot De Spiegeleer, Vera Plekhova, Jozefien Geltmeyer, Ella Schoolaert, Beata Pomian, Varoon Singh, Kathleen Wijnant, Kimberly De Windt, Volter Paukku, Alexander De Loof, Inge Gies, Nathalie Michels, Stefaan De Henauw, Marilyn De Graeve, Karen De Clerck, Lynn Vanhaecke
Summary: In recent years, ambient ionization mass spectrometry (AIMS) has allowed for direct analysis of biofluid metabolome. However, AIMS procedures are hindered by analytical and practical limitations, which affect metabolome coverage. This study aimed to develop biofluid-specific metabolome sampling membranes (MetaSAMPs) to improve AIMS. Customized MetaSAMPs made of hydrophilic and lipophilic polymers demonstrated superior metabolome coverage and transport stability compared to crude biofluid analysis. This new approach was successfully validated and has potential applications in metabolic health stratification.
Article
Chemistry, Multidisciplinary
Chiranjit Ghosh, Varoon Singh, Jonathan Grandy, Janusz Pawliszyn
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
