Article
Chemistry, Analytical
Ying Zhang, Bei Gao, Luis Valdiviez, Chao Zhu, Tara Gallagher, Katrine Whiteson, Oliver Fiehn
Summary: Stable isotope tracers are commonly used with gas chromatography coupled to mass spectrometry for enzyme activity and metabolic pathway studies. In this research, three commercially available instruments were used to analyze samples from a stable isotope labeling study on Rothia mucilaginosa metabolism. The study found that all three GC-MS instruments can be used for stable isotope tracing studies, with high-resolution GC-QTOF MS offering additional capabilities in identifying the chemical structures of unknown compounds.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Ying Shi, Huang-Fei Jin, Min-Zhen Shi, Jun Cao, Li-Hong Ye
Summary: In this study, a miniaturized solid phase extraction method and supercritical fluid chromatography combined with ion mobility quadrupole time-of-flight mass spectrometry were used for the qualitative and quantitative analysis of carbamate pesticide residues. The method was validated and applied successfully to the enrichment and detection of carbamate residues in ginger samples.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Chu -Fan Wang, Liang Li
Summary: Chemical isotope labeling (CIL) LC-MS is a powerful tool for metabolome analysis that offers improved coverage and accuracy. This study compares quadrupole time-of-flight (QTOF) and Orbitrap mass spectrometers for metabolite detection in CIL LC-MS. The results show that instrument type has a significant effect on metabolite detection, and combining data from different instruments can increase the number of detected peak pairs.
ANALYTICA CHIMICA ACTA
(2022)
Article
Agriculture, Multidisciplinary
Johannes Wellmann, Conny Traenkner, Nicolas Dostert, Esther-Corinna Schwarze, Silke Hillebrand, Jakob P. Ley, Peter Winterhalter
Summary: A workflow for comprehensive semi-polar metabolite profiling was developed and validated using liquid chromatography coupled with electrospray ionization ion mobility quadrupole time-of-flight mass spectrometry. This workflow has the capability to distinguish different cultivars of Hydrangea macrophylla ssp. serrata and obtain a list of metabolites responsible for the differentiation. Suspect screening of phenyldihydroisocoumarins provides valuable information for the biosynthesis of phyllodulcin.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Food Science & Technology
Zi-Xuan Yue, Jun Cao
Summary: In this study, matrix solid phase dispersion (MSPD) microextraction combined with supercritical fluid chromatography-ion mobility quadrupole time-of-flight mass spectrometry (SFC/IM-QTOF-MS) was used to analyze the multipolar compounds in plant tea. The method achieved good separation and quantitative analysis of active ingredients in tea.
Article
Chemistry, Applied
Hui Zheng, Xiao-Ting Zhen, Yan Chen, Si-Chen Zhu, Li-Hong Ye, Si-Wei Yang, Qiu-Yan Wang, Jun Cao
Summary: A simple and sensitive in situ antioxidation process combined with matrix solid-phase dispersion method was established for extracting chiral flavonoids in citrus fruit. The method showed satisfactory limits of detection and good recoveries for the four target compounds. The IC50 values of DPPH radical-scavenging activities were obtained to evaluate the antioxidant capacity of the samples.
Article
Food Science & Technology
Shizuka Saito-Shida, Satoru Nemoto, Hiroshi Akiyama
Summary: The study demonstrated the applicability of LC-QTOF-MS method for quantitative and confirmatory analysis of pesticide residues in cereal grains and legumes, with high signal intensities and accuracy, suitable for regulatory routine analysis.
Article
Food Science & Technology
Jian Li, Junmei Ma, Yan Zhang, Lei Zheng
Summary: A rapid method was developed for the determination of 19 polyphenols in tea using ultra-high performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry (UPLC-Q-TOF MS). The method exhibited simplicity, rapidity, and accuracy, making it suitable for the rapid screening and quantitative analysis of polyphenols in tea.
FOOD SCIENCE AND HUMAN WELLNESS
(2022)
Article
Environmental Sciences
Sofia Barreales-Suarez, Stephane Azoulay, Miguel Angel Bello-Lopez, Rut Fernandez-Torres
Summary: In this study, an uptake assay of pharmaceuticals by Juncus sp. and Salicornia europaea was conducted at three concentration levels. The results showed that plants irrigated at high levels of pharmaceuticals were significantly affected, while plants at other levels displayed no apparent physiological effects. Ibuprofen was not detected in any sample, and some pharmaceuticals were not detected in Salicornia europaea samples.
Article
Biochemical Research Methods
M. Cobo-Golpe, M. Ramil, R. Cela, I. Rodriguez
Summary: This article presents a fast and sensitive chiral analysis method for determining eight pharmaceuticals in wastewater and sludge samples. The compounds are separated using supercritical fluid chromatography combined with time-of-flight mass spectrometry detection. Methanol and ammonia are used as modifiers in the mobile phase. The method allows for the analysis of eight pairs of enantiomers in 15.5 minutes and has been successfully applied to the analysis of wastewater and sludge samples.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Analytical
Xiaowei Zhang, Xinpeng Diao, Luya Li, Yuqian Zhang, Man Liao, Guohua Zhang, Lantong Zhang
Summary: This study analyzed the metabolic profile of ginkgolide B using ultra-high-performance liquid chromatography quadrupole time-of-flight mass spectrometry, and identified a total of 53 different metabolites. The biotransformation route of ginkgolide B was determined, providing useful information for further research on its pharmacology and mechanism.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Engineering, Environmental
Peng Li, Yujian Lai, Rong-gang Zheng, Qing-cun Li, Xueying Sheng, Sujuan Yu, Zhineng Hao, Ya-qi Cai, Jingfu Liu
Summary: A novel method combining TMAH digestion and DCM dissolution has been developed to extract small microplastics and nanoplastics embedded in soils, sediments, and sludges. The feasibility of this method has been verified and it shows promise for evaluating small solid-embedded microplastic and nanoplastic pollution.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Chemistry, Analytical
Xiao-wei Zhang, Yu-ting Chen, Xue Feng, Lu-ya Li, Ke-wei Song, Yu-peng Sun, Guo-hua Zhang, Lan-tong Zhang
Summary: In this study, the metabolism of celastrol in vivo and in vitro was comprehensively characterized using ultra-high-performance liquid chromatography coupled with hybrid triple quadrupole time-of-flight mass spectrometry. A total of 43 metabolites were identified and characterized, and it was found that the biotransformation capacity of the intestinal tract exceeded that of the liver. These findings provide a basis for understanding the pharmacological mechanisms of celastrol and have potential implications for clinical applications.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Chemistry, Analytical
Li-Hong Ye, Juan Yang, Fang-Ming Liu, Jun Cao
Summary: A method for identification and difference analysis of active compounds in different species of hawthorn using ultrahigh-performance liquid chromatography coupled with mass spectrometry was proposed. The study investigated and optimized various parameters for separation and response, and successfully distinguished different species of hawthorn using mass spectrometry data combined with multivariate analysis.
MICROCHEMICAL JOURNAL
(2021)
Article
Agricultural Engineering
Zainab Syed, Monika Sogani, Anu Kumar, Jayana Rajvanshi, Gopesh Sharma, Kumar Sonu
Summary: This research focused on the biodegradation of synthetic estrogenic compound 17 alpha-ethinylestradiol (EE2) and compared its degradation efficiency and power output in suspension flasks and a Bioelectrochemical System (BES). The results showed that the BES had significantly higher EE2 removal rate and power density. The study also identified a potential pathway for EE2 degradation using the newly isolated strain Rhodopseudomonas palustris MDOC01, and detected important metabolites during the bioelectrochemical treatment process of EE2.
BIORESOURCE TECHNOLOGY
(2022)
Article
Agricultural Engineering
T. Rodriguez-Cabo, I. Rodriguez, M. Ramil, R. Cela
INDUSTRIAL CROPS AND PRODUCTS
(2018)
Article
Biochemical Research Methods
Ariel Fontana, Isaac Rodriguez, Rafael Cela
JOURNAL OF CHROMATOGRAPHY A
(2018)
Article
Environmental Sciences
Iria Gonzalez-Marino, Isaac Rodriguez, Lorena Rojo, Rafael Cela
SCIENCE OF THE TOTAL ENVIRONMENT
(2018)
Article
Environmental Sciences
T. Rodriguez-Cabo, I. Rodriguez, M. Ramil, R. Cela
SCIENCE OF THE TOTAL ENVIRONMENT
(2018)
Article
Biochemical Research Methods
Gabriela Castro, Inmaculada Carpinteiro, Isaac Rodriguez, Rafael Cela
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2018)
Article
Biochemical Research Methods
L. Perez-Mayan, I. Rodriguez, M. Ramil, A. Kabir, K. G. Furton, R. Cela
JOURNAL OF CHROMATOGRAPHY A
(2019)
Article
Environmental Sciences
I Carpinteiro, G. Castro, I Rodriguez, R. Cela
SCIENCE OF THE TOTAL ENVIRONMENT
(2019)
Article
Environmental Sciences
Rosario Rodil, Eugenia Villaverde-de-Saa, Julio Cobas, Jose Benito Quintana, Rafael Cela, Nieves Carro
ENVIRONMENT INTERNATIONAL
(2019)
Article
Biochemical Research Methods
Veronica Castro, Rosario Rodil, Jose Benito Quintana, Rafael Cela, Laura Sanchez-Fernandez, Iria Gonzalez-Marino
JOURNAL OF CHROMATOGRAPHY A
(2019)
Article
Biochemical Research Methods
S. Trinanes, P. Rodriguez-Mier, C. Cobas, E. Sanchez, R. Phan-Tan-Luu, R. Cela
JOURNAL OF CHROMATOGRAPHY A
(2020)
Article
Biochemical Research Methods
L. Perez-Mayan, M. Cobo-Golpe, M. Ramil, R. Cela, I Rodriguez
JOURNAL OF CHROMATOGRAPHY A
(2020)
Article
Biochemical Research Methods
G. Castro, L. Perez-Mayan, I Carpinteiro, M. Ramil, R. Cela, I Rodriguez
JOURNAL OF CHROMATOGRAPHY A
(2020)
Article
Environmental Sciences
L. Perez-Mayan, M. Ramil, R. Cela, I Rodriguez
Article
Biochemical Research Methods
R. Cela, S. Trinanes, C. Cobas
Summary: A new strategy has been developed for computer-assisted methods in reversed-phase liquid chromatographic separations, using latent spectral information in chromatogram raw data files. By utilizing multivariate curve resolution-alternating least squares (MCR-ALS), this technique allows estimation of true spectra for individual components, providing a general approach for chromatography laboratory tools. This strategy is applicable to both known and unknown sample compositions, with the main difference being the need for an initial spectrum discovery process for unknown mixtures.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Gabriela Castro, Leticia Perez-Mayan, Tamara Rodriguez-Cabo, Isaac Rodriguez, Maria Ramil, Rafael Cela
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2018)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)