Article
Chemistry, Medicinal
Yang Wang, Ge Gao, Xiangpin Wei, Yang Zhang, Jian Yu
Summary: UBE2T plays a crucial role in mediating resistance of glioblastoma cells to temozolomide (TMZ) treatment by regulating the Wnt/I3-catenin signaling pathway. Inhibition of UBE2T sensitizes GBM cells to TMZ, while UBE2T overexpression promotes TMZ resistance. Targeting UBE2T may be a potential strategy to overcome TMZ resistance in GBM.
DRUG DESIGN DEVELOPMENT AND THERAPY
(2023)
Article
Chemistry, Multidisciplinary
Reena Jatyan, Deepak Kumar Sahel, Prabhjeet Singh, Rajeev Sakhuja, Anupama Mittal, Deepak Chitkara
Summary: In this study, the conjugation of fatty acids with temozolomide improved its anticancer potential against glioblastoma multiforme. The synthesized conjugates showed a stronger inhibitory effect on tumor cells in vitro and improved survival rate in an animal model.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Biochemistry & Molecular Biology
Yan Lu, Meng Tian, Jiongbo Liu, Kuanhong Wang
Summary: The study identified LINC00511 as a lncRNA associated with TMZ resistance in GBM, upregulated in resistant cells and correlated with poor prognosis in patients. LINC00511 was found to regulate Wnt/beta-catenin signaling, impacting TMZ sensitivity in GBM cells. These findings suggest LINC00511 as a potential therapeutic target and biomarker for GBM patients.
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Zhourui Ma, Shizhong Cai, Qianwei Xiong, Wei Liu, Hongliang Xia, Zhenhong Zhu, Zhijian Huang, Xiangming Yan, Qian Wang
Summary: This study reveals the role of WNT signaling in the chemoresistance of glioblastoma multiforme (GBM) to temozolomide (TMZ). The loss of function of p53 in GBM downregulates miR-34a expression, leading to the activation of WNT signaling and the resistance to TMZ. By targeting the miR-34a/WNT6 axis, drug sensitivity of p53-mutant GBM cells can be restored and overall survival can be extended.
Review
Biochemistry & Molecular Biology
Michael Latour, Nam-Gu Her, Santosh Kesari, Elmar Nurmemmedov
Summary: The WNT signaling pathway plays a crucial role in glioblastoma genesis and therapy resistance, presenting a therapeutic challenge. Current targeted therapies under clinical investigation may offer new treatment options.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Ophthalmology
Yueru Li, Basma Baccouche, Olamide Olayinka, Anara Serikbaeva, Andrius Kazlauskas
Summary: The study reveals that VEGF/anti-VEGF controls endothelial cell permeability by regulating multiple members of the Wnt pathway, and activating this pathway helps strengthen the endothelial cell barrier. FDA-approved agents such as LiCl may serve as adjuvants to anti-VEGF therapy for blinding conditions like diabetic retinopathy.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Yan Zou, Yibin Wang, Sen Xu, Yanjie Liu, Jinlong Yin, David B. Lovejoy, Meng Zheng, Xing-Jie Liang, Jong Bae Park, Yuri M. Efremov, Ilya Ulasovand, Bingyang Shi
Summary: A new type of GBM-cell membrane camouflaged and pH-sensitive biomimetic nanoparticles have been developed for combined TMZ and CDDP therapy for GBM. The results show that these nanoparticles can efficiently transport drugs across the blood-brain barrier and specifically target GBM, allowing for controlled release at GBM sites and demonstrating potent anti-GBM effects without obvious side effects.
ADVANCED MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Marlyn D. Laksitorini, Vinith Yathindranath, Wei Xiong, Fiona E. Parkinson, James A. Thliveris, Donald W. Miller
Summary: Chronic exposure to ethanol can enhance blood-brain barrier permeability, but this effect can be reversed by activating the Wnt signaling pathway.
JOURNAL OF NEUROCHEMISTRY
(2021)
Article
Pharmacology & Pharmacy
Zhuqing Song, Xiuxian Huang, Jieqiong Wang, Feiyan Cai, Ping Zhao, Fei Yan
Summary: The ultrasound-mediated BBB opening combined with liposomal temozolomide formulation has been successfully applied in the treatment of glioblastoma, significantly inhibiting tumor growth and prolonging animal survival time without significant side effects.
Article
Biochemistry & Molecular Biology
Yan-Qing Fei, Ru-Ting Shi, Yang-Fan Zhou, Jin-Ze Wu, Zhi Song
Summary: Mannose inhibits glioma cell growth and enhances sensitivity to TMZ, possibly through the inhibition of MGMT and activation of the Wnt/beta-catenin pathway.
NEUROCHEMISTRY INTERNATIONAL
(2022)
Article
Biochemistry & Molecular Biology
Amir Barzegar Behrooz, Zahra Talaie, Fatemeh Jusheghani, Marek J. Los, Thomas Klonisch, Saeid Ghavami
Summary: Glioblastoma (GBM) is a devastating type of brain tumor, and targeting the Wnt/β-catenin and PI3K/Akt/mTOR (PAM) signaling pathways, which play crucial roles in GBM progression and invasion, may provide important advancements in alternative treatment strategies for GBM.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Philippe Aretz, Donata Maciaczyk, Suad Yusuf, Rudiger V. Sorg, Daniel Haenggi, Hongjia Liu, Hongde Liu, Tikam Chand Dakal, Amit Sharma, Ramakrishna Bethanabatla, Silke Neumann, Jarek Maciaczyk
Summary: The study reveals the crucial role of beta-catenin in attracting monocytes towards GBM cells and suggests that the interaction between beta-catenin and CCL2 may contribute to the maintenance of GSCs by modulating immune cell interaction and promoting GBM growth and recurrence.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Shengchao Xu, Chengke Luo, Dikang Chen, Lu Tang, Quan Cheng, Ling Chen, Zhixiong Liu
Summary: The study aimed to elucidate the role and mechanism of circMMD in tumor treating fields (TTF) treatment of glioblastoma (GBM). The results showed that circMMD expression was elevated in GBM and high expression was associated with poor prognosis. TTF intervention reduced circMMD synthesis, suppressing GBM proliferation and increasing TTF-mediated apoptosis. The reduction of circMMD promoted the interaction between FUBP1 and FIR, leading to decreased DVL1 transcription. Additionally, decreased circMMD enhanced miR-15b-5p activity to degrade FZD6. Ultimately, the diminished expression of DVL1 and FZD6 suppressed the activation of Wnt/beta-catenin pathway.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2023)
Article
Oncology
Dandan Fan, Qi Yue, Jian Chen, Cong Wang, Ruilin Yu, Ziyi Jin, Shujie Yin, Qinyue Wang, Luo Chen, Xueling Liao, Chengyuan Peng, Jianpin Zhang, Zhonglian Cao, Ying Mao, Ruimin Huang, Liang Chen, Cong Li
Summary: The study demonstrated that immunosuppressive genes were upregulated in IDH1(WT) glioma patients, and blocking Wnt/beta-catenin signaling improved the hostile immunosuppressive microenvironment, enhancing the treatment efficacy for IDH1(WT) GBM.
Article
Environmental Sciences
Ai Mai, Shu-Wen Ye, Jia-Yu Tu, Jun Gao, Zhan-Fang Kang, Qian-Ming Yao, Wei-Jen Ting
Summary: This study demonstrates that thymoquinone (TQ) can effectively cross the blood-brain barrier and has therapeutic potential for the treatment of glioblastoma, especially when used in combination with temozolomide (TMZ).
ENVIRONMENTAL TOXICOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Magdalena Socha, Anna Szoszkiewicz, Dorota Simon, Aleksander Jamsheer
Summary: Partial 16p trisomy syndrome is a rare disorder characterized by psychomotor retardation, growth deficiency, cleft palate, and facial dysmorphism. We report a patient with this syndrome who carries a complex de novo 16p13.3 microduplication without translocation or associated monosomy. The duplicated region does not involve CREBBP and neighboring genes, but the patient exhibits typical dysmorphic features.
JOURNAL OF APPLIED GENETICS
(2023)
Article
Biochemistry & Molecular Biology
Magdalena Paczkowska-Walendowska, Andrzej Miklaszewski, Daria Szymanowska, Krystyna Skalicka-Wozniak, Judyta Cielecka-Piontek
Summary: Hot Melt Extrusion (HME) technology was developed to improve the rheological properties of blends containing lyophilized Scutellariae baicalensis root extract and chitosan through the use of different ratios of (Hydroxypropyl)methyl cellulose (HPMC) as amorphous matrix formers. The systems were characterized and tablets were prepared from the extrudates. HPMC-based systems showed delayed release of baicalin due to the swelling behavior of HPMC, and the best tabletability properties were observed in the formulation containing lyophilized extract HPMC 50:50 w/w, which maintained good mucoadhesive properties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Kamil Wdowiak, Andrzej Miklaszewski, Robert Pietrzak, Judyta Cielecka-Piontek
Summary: The aim of this study was to prepare and characterize the amorphous dispersions of hesperetin and piperine to enhance their solubility and bioavailability. The amorphous systems were successfully obtained through ball milling and confirmed by XRPD and DSC studies. Amorphization significantly improved the dissolution rate, apparent solubility, permeability, and biological activities of hesperetin and piperine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Robert Kleszcz, Mikolaj Frackowiak, Dawid Dorna, Jaroslaw Paluszczak
Summary: The aim of this study was to investigate the effects of the combinatorial use of Wnt/beta-catenin and Hedgehog pathway inhibitors on head and neck cancer cells. The results showed that the combination of PRI-724 and vismodegib affected cell cycle distribution, reduced cell migration, and downregulated the expression of CSC markers. Combining PRI-724 with erlotinib or HS-173 was more effective in inducing apoptosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Magdalena Paczkowska-Walendowska, Andrzej Miklaszewski, Judyta Cielecka-Piontek
Summary: The optimization of hesperidin extraction from orange peel was conducted to obtain its highest content, by investigating the influence of extraction mixture and temperature. The results showed that methanol and temperature were key parameters for efficient extraction of bioactive compounds. Furthermore, electrospinning technology was used to prepare nanofibers loaded with hesperidin-rich orange peel extract, which exhibited improved solubility and permeability. These nanofibers demonstrated significant antioxidant and anti-inflammatory activities, suggesting their potential buccal applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Natalia Rosiak, Judyta Cielecka-Piontek, Robert Skibinski, Kornelia Lewandowska, Waldemar Bednarski, Przemyslaw Zalewski
Summary: The influence of ionizing radiation on the physicochemical properties of quercetin and rutin in the solid state was investigated. The results showed that irradiation caused the formation of a small number of free radicals, but did not change the chemical structure or antioxidant properties of the tested flavonoids. Spectroscopic and chromatographic analyses confirmed the radiostability of quercetin and rutin.
Article
Pharmacology & Pharmacy
Natalia Rosiak, Ewa Tykarska, Judyta Cielecka-Piontek
Summary: The aim of this study was to improve the solubility and antioxidant activity of pterostilbene (PTR) by developing a novel amorphous solid dispersion (ASD) with Soluplus (R) (SOL). Three appropriate PTR and SOL weight ratios were selected using DSC analysis and mathematical models. The amorphization process was carried out by dry milling, resulting in complete loss of crystallinity and improved apparent solubility and antioxidant activity of PTR after introduction into ASD.
Article
Biochemistry & Molecular Biology
Anna Stasilowicz-Krzemien, Natalia Rosiak, Andrzej Miklaszewski, Judyta Cielecka-Piontek
Summary: To improve the bioavailability of caffeic acid, delivery systems were developed using ball milling and freeze-drying techniques to prepare solid dispersions of caffeic acid and magnesium aluminometasilicate. The 1:1 mass ratio solid dispersion obtained from ball milling was the most effective, and its identity was confirmed using X-ray powder diffraction and Fourier-transform infrared spectroscopy techniques. Screening tests demonstrated the improved anti-neurodegenerative activity of caffeic acid with enhanced solubility, inhibiting acetylcholinesterase, butyrylcholinesterase, tyrosinase, and showing antioxidant potential. In silico studies identified the domains of caffeic acid that interacted with enzymes relevant to neuroprotective activity. Furthermore, improved permeability through gastrointestinal tract and blood-brain barrier mimicking membranes validated the in vivo screening tests for anti-neurodegenerative activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Anna Stasilowicz-Krzemien, Piotr Szulc, Judyta Cielecka-Piontek
Summary: To improve the bioavailability of cannabinoids upon oral administration, researchers have developed co-dispersion cannabinoid delivery systems using biopolymers and carriers, which enhances the solubility and permeability of cannabinoids. The results demonstrate that the co-dispersion cannabinoid delivery systems have improved dissolution and permeability, showing promise for increased bioavailability of cannabinoids.
Article
Biochemistry & Molecular Biology
Magdalena Paczkowska-Walendowska, Andrzej Miklaszewski, Bozena Michniak-Kohn, Judyta Cielecka-Piontek
Summary: Despite the wide range of effects of polyphenols, their low oral bioavailability limits their usefulness. In this study, red vine leaf extract with optimal antioxidant and anti-inflammatory properties was obtained using the Design of Experiment approach. Nanofibers containing the extract were then developed to improve the release and buccal penetration of resveratrol. This innovative strategy enhances the utilization of active compounds for the development of nutraceuticals.
Article
Biochemistry & Molecular Biology
Anna Stasilowicz-Krzemien, Szymon Sip, Piotr Szulc, Judyta Cielecka-Piontek
Summary: Cannabis leaves contain a range of antioxidants that offer health benefits, and utilizing them can be cost-effective. This study aimed to assess the antioxidant activity of hemp leaves from different cultivars and analyze their cannabinoid profiles. The choice of extractant and extraction conditions significantly influenced the extraction efficiency and antioxidant activity of the active compounds. Ultrasonic-assisted extraction using methanol yielded the highest cannabinoid profile and antioxidant potential in Bialobrzeska leaf extracts.
Article
Biochemistry & Molecular Biology
Anna Stasilowicz-Krzemien, Judyta Cielecka-Piontek
Summary: Lupuli flos extracts exhibit significant antioxidant and enzyme inhibitory activities for neuroprotection. The extract obtained under 5000 PSI and 50 degrees C shows the most beneficial neuroprotective effects. Hydroxypropyl-beta-cyclodextrin in combination with magnesium aluminometasilicate shows the greatest improvement in dissolution profiles for the active compounds.
Article
Biochemistry & Molecular Biology
Anna Stasilowicz-Krzemien, Szymon Sip, Piotr Szulc, Jaroslaw Walkowiak, Judyta Cielecka-Piontek
Summary: Cannabis sativa, a versatile plant with promising potential for various biological activities, was extracted using supercritical carbon dioxide. Cannabinoids, particularly cannabidiol (CBD) and cannabidiolic acid (CBDA), were the dominant compounds found in the extracts. Different hemp varieties exhibited varied antioxidant and neuroprotective properties.
Article
Chemistry, Medicinal
Magdalena Paczkowska-Walendowska, Lidia Tajber, Andrzej Miklaszewski, Judyta Cielecka-Piontek
Summary: This study prepared solid dispersions containing polydatin-rich Polygoni cuspidati extract by hot melt extrusion, aiming to enhance the release of poorly water-soluble compounds. The formulation based on Kollidon (R) VA64 exhibited the best tabletability properties and mucoadhesive properties.
Article
Medicine, General & Internal
Malgorzata Jamka, Agata Czochralska-Duszynska, Edyta Madry, Aleksandra Lisowska, Katarzyna Jonczyk-Potoczna, Judyta Cielecka-Piontek, Pawel Bogdanski, Jaroslaw Walkowiak
Summary: This study aimed to evaluate the effects of CLA supplementation on bone mineral density and content in overweight or obese women. The results showed that three-month CLA supplementation did not improve bone health in this population. However, long-term intervention studies are needed to further investigate the potential benefits of CLA on bone health.
MEDICINA-LITHUANIA
(2023)