Review
Critical Care Medicine
Penny S. Reynolds, Cynthia W. Garvan
Summary: The study found that there are significant deficiencies in animal-based research reporting, including lack of ethical oversight information, basic animal descriptive information, and best-practice methods. Researcher need to enhance transparency, improve experimental design skills to promote the quality of research method reporting.
Review
Medicine, General & Internal
Xi Peng, Guocheng Rao, Xinqiong Li, Nanwei Tong, Yan Tian, Xianghui Fu
Summary: This article comprehensively summarizes and discusses the applications, advantages, and limitations of commonly used animal models for human T1DM, as well as provides an overview of the latest human tissue bioengineering models for T1DM research. By combining these models with a better understanding of the pathophysiology of T1DM, we can gain deeper insights into disease development and ultimately improve therapeutic responses and outcomes.
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
(2023)
Review
Metallurgy & Metallurgical Engineering
Yu Sun, Heike Helmholz, Regine Willumeit-Roemer
Summary: Degradable magnesium implants show promise for fracture treatment, but there are considerable variations in model selection and study design, highlighting the need for a standardized reference model. This systematic review aims to provide comprehensive guidance for future preclinical research on magnesium implants for fracture treatment.
JOURNAL OF MAGNESIUM AND ALLOYS
(2021)
Review
Immunology
Yinxi Zhou, Jinghua Xia, Shuonan Xu, Tao She, Yanning Zhang, Ying Sun, Miaomiao Wen, Tao Jiang, Yanlu Xiong, Jie Lei
Summary: The development and growth of tumors pose a significant and ongoing threat to human life globally. Despite the remarkable progress achieved by advanced therapeutic strategies such as immune checkpoint therapy and CAR-T in treating solid and hematological malignancies, the malignant initiation and progression of cancer remains controversial and requires further research. Experimental animal models not only have great advantages in simulating tumor occurrence, development, and malignant transformation mechanisms, but also can be used to evaluate the therapeutic effects of diverse clinical interventions, gradually becoming indispensable in cancer research. This paper reviews recent research progress in mouse and rat models, focusing on spontaneous, induced, transgenic, and transplantable tumor models, aiming to provide guidance for future studies on malignant mechanisms and tumor prevention.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Medicine, Research & Experimental
Suryapriya Ulaganathan, Arunkumar Pitchaimani
Summary: Alzheimer's disease (AD) is a progressive and irreversible neurodegenerative disorder, and understanding its underlying mechanisms is still a challenge. Current animal models fail to accurately mimic the pathology of AD, both in familial and sporadic variants. This review highlights the gaps in AD models and discusses recent advancements in in vitro and chimeric AD models.
Review
Clinical Neurology
Sarinnapha M. Vasunilashorn, Nadia Lunardi, John C. Newman, Gregory Crosby, Leah Acker, Ted Abel, Seema Bhatnagar, Colm Cunningham, Rafael de Cabo, Laura Dugan, Joseph A. Hippensteel, Yumiko Ishizawa, Shouri Lahiri, Edward R. Marcantonio, Zhongcong Xie, Sharon K. Inouye, Niccolo Terrando, Roderic G. Eckenhoff
Summary: Delirium is a common, morbid, and costly syndrome closely associated with Alzheimer's disease (AD) and AD-related dementias (ADRD). While human studies have improved our understanding of delirium in terms of incidence, risk factors, outcomes, and management, limited knowledge exists about the neurobiology of delirium. This article discusses the use of preclinical and translational animal models to advance our understanding of delirium neurobiology and develop new prevention and treatment strategies. Recommendations for the development and validation of these models are provided to enhance awareness in this important field.
ALZHEIMERS & DEMENTIA
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Yunjie Yang, Minglei Kang, Chin-Cheng Chen, Lei Hu, Francis Yu, Pingfang Tsai, Sheng Huang, Jiayi Liu, Ryan Turner, Brian Shen, Shaakir Hasan, Arpit M. Chhabra, J. Isabelle Choi, Brett Bell, Michael Pennock, Wolfgang A. Tome, Chanda Guha, Charles B. Simone, Haibo Lin
Summary: A 250 MeV proton research beamline was successfully commissioned and characterized for proton FLASH-RT small animal experiments. The study found that a saturated nozzle monitor ionization chamber can achieve the desired dose and scaling the MU can overcome the challenges caused by the saturation. Additionally, a simple aperture system was designed and validated to provide sharp dose fall-off for small animal experiments. These findings are important for other centers interested in implementing FLASH radiotherapy preclinical research.
Article
Orthopedics
Chengchong Ai, Yee Han Dave Lee, Xuan Hao Tan, Si Heng Sharon Tan, James Hoi Po Hui, James Cho-Hong Goh
Summary: The treatment of osteochondral defects remains challenging, with no consensus on the clinical effectiveness of scaffold-based therapies. This review focuses on bridging the gap between clinical need and preclinical research, providing insight into improving tissue engineering methods. It summarizes current surgical treatments, reviews recent animal studies, and highlights the need for further improvement in these techniques.
JOURNAL OF ORTHOPAEDIC TRANSLATION
(2021)
Article
Dentistry, Oral Surgery & Medicine
Ji-Yeong Lee, Sangyup Kim, Seung-Yun Shin, Jong-Hyuk Chung, Yeek Herr, Hyun-Chang Lim
Summary: In this study, hydraulic pressure-assisted sinus augmentation led to new bone formation in areas distant from the bone access hole, but showed a similar histological healing pattern to conventional sinus augmentation in a rabbit sinus model.
CLINICAL ORAL INVESTIGATIONS
(2022)
Review
Immunology
Min Zhou, Yucheng Tang, Wenjie Xu, Xinyan Hao, Yongjiang Li, Si Huang, Daxiong Xiang, Junyong Wu
Summary: Immunotherapy based on bacteria has shown promising potential in cancer management by modulating immune response through various cellular and molecular pathways. Bacterial antigens synthesized as vaccine candidates offer a novel approach for antitumor immunotherapy. Combination therapy with bacterial vaccine and conventional treatments may enhance therapeutic benefits against cancer. However, the clinical translation of bacteria-based immunotherapy faces challenges regarding biosafety and production standards.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Engineering, Biomedical
Peyman Karami, Theofanis Stampoultzis, Yanheng Guo, Dominique P. Pioletti
Summary: The drive to develop cartilage implants for major defects in the musculoskeletal system has led to an increased focus on developing biomaterial devices for cartilage repair. However, there has been limited attention on the preclinical validation process and approval procedures for these devices. This review paper aims to highlight the various considerations regarding preclinical validation of hydrogel devices for cartilage repair, including regulatory requirements, implantation strategies, device performance aspects, and characterizations.
ACTA BIOMATERIALIA
(2023)
Article
Microbiology
Nhu T. Q. Nguyen, Emmanuelle Gras, Nguyen D. Tran, Nhi N. Y. Nguyen, Hanh T. H. Lam, William J. Weiss, Thien N. M. Doan, Binh An Diep
Summary: The study developed a rabbit model that mimics the clinical features of acute respiratory distress syndrome (ARDS) and evaluated its predictive power for antibiotic efficacy testing. The results showed that a humanized dosing regimen of meropenem with intensive care unit supportive care achieved the highest level of survival, halted the worsening of ARDS biomarkers, and reversed lethal hypotension in the rabbit model.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Dentistry, Oral Surgery & Medicine
Yusuke Hori, Yusuke Kondo, Tomotaka Nodai, Chihiro Masaki, Kentaro Ono, Ryuji Hosokawa
Summary: The study found that while xerostomia itself did not induce bone resorption around implants, it significantly enhanced bone resorption caused by silk ligatures. Histological analysis and real-time PCR showed that xerostomia induced inflammation and osteoclastogenesis around implants with silk ligatures, as well as altered the microbiota on the plaque around implants. Xerostomia was concluded to accelerate mucosal inflammation and osteoclastogenesis, leading to aggravated bone resorption around implants.
CLINICAL ORAL IMPLANTS RESEARCH
(2021)
Article
Biotechnology & Applied Microbiology
Xuyang Zhang, Minglu Xu, Qin Xue, Yao He
Summary: We have modified the rat ligature periodontitis model by fixing the thread ligature on the metal steel ligature. The modified model showed the pathological process of periodontitis and demonstrated efficiency and reliability in inducing rat periodontitis. Compared to the thread ligature model and the metal steel ligature model, the modified model was more reliable and had greater ability of bacterial aggregation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Materials Science, Biomaterials
Fengxing Ding, Kaiyan Hu, Xia Liu, Chen Liu, Jinwei Yang, Xinli Shi, Bin Liu, Mei Wu, Zhe Wang, Liyuan Feng, Jiazhen Zhang, Bin Ma
Summary: In this study, the reporting quality of in vivo investigations on the use of degradable metal materials in fracture or bone defect repair was assessed. The results showed that the published experiments lacked transparent, accurate, and comprehensive reporting on key elements of the experimental design and other elements meant to avoid bias.
REGENERATIVE BIOMATERIALS
(2022)
Article
Oncology
Kavita Bisht, Crystal McGirr, Seo-Youn Lee, Hsu-Wen Tseng, Whitney Fleming, Kylie A. Alexander, Taichi Matsumoto, Valerie Barbier, Natalie A. Sims, Gerhard Mueller-Newen, Ingrid G. Winkler, Halvard Bonig, Jean-Pierre Levesque
Summary: The study demonstrates that pro-inflammatory cytokine OSM plays a crucial role in regulating HSC niches in the bone marrow. OSM acts as a negative feedback to inhibit HSPC mobilization and influences HSC homing and proliferation indirectly.
Article
Endocrinology & Metabolism
Hsu-Wen Tseng, Irina Kulina, Dorothee Girard, Jules Gueguen, Cedryck Vaquette, Marjorie Salga, Whitney Fleming, Beulah Jose, Susan M. Millard, Allison R. Pettit, Kate Schroder, Gethin Thomas, Lawrie Wheeler, Francois Genet, Sebastien Banzet, Kylie A. Alexander, Jean-Pierre Levesque
Summary: Neurogenic heterotopic ossifications (NHO) occur in periarticular muscles following severe spinal cord and traumatic brain injuries, with the pathogenesis poorly understood. Research suggests that IL-1 signaling contributes to NHO development after SCI in mice, while other inflammation-related proteins were not found to promote NHO development. Biopsies from NHO patients indicate that IL-1 beta is expressed by macrophages, and IL-1 promotes NHO development by enhancing calcium mineralization and RUNX2 expression in fibro-adipogenic progenitors.
JOURNAL OF BONE AND MINERAL RESEARCH
(2022)
Review
Endocrinology & Metabolism
Hsu-Wen Tseng, Selwin Gabriel Samuel, Kate Schroder, Jean-Pierre Levesque, Kylie A. Alexander
Summary: Inflammasomes play a crucial role in regulating bone dynamics, and animal models have been instrumental in understanding their impact on skeletal health. Dysregulation of inflammasomes is associated with bone pathologies, highlighting the importance of tightly regulating these protein structures for skeletal homeostasis.
CURRENT OSTEOPOROSIS REPORTS
(2022)
Article
Engineering, Biomedical
Agathe Bessot, Jennifer Gunter, David Waugh, Judith A. Clements, Dietmar W. Hutmacher, Jacqui McGovern, Nathalie Bock
Summary: Increasing evidence highlights the important role of bone marrow adipocytes in prostate cancer bone metastases. However, lack of relevant models has hindered the understanding of human BM-adipocyte effects in this microenvironment. This study combines tunable GelMA-based hydrogels with biomimetic culture of human cells to engineer human bone tumor microenvironments containing BM-adipocytes. In vitro culture of human osteoprogenitors, adipocytes, and PCa cells in GelMA hydrogels leads to the formation of mineralized, adipose, and PCa tumor 3D microtissues, respectively. The stiffness of hydrogels regulates osteoblast mineralization and tumor spheroid formation, while coculture with tumor cells induces morphological changes and delipidation in BM-adipocytes. The in vivo model successfully forms a humanized fatty bone microenvironment and demonstrates that adipocytes support early bone tumor growth. This advanced platform combines natural aspects of the microenvironment with tunable properties for bone tumor research.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Engineering, Biomedical
Verena Kast, Ali Nadernezhad, Dagmar Pette, Anastasiia Gabrielyan, Maximilian Fusenig, Kim C. Honselmann, Daniel E. Stange, Carsten Werner, Daniela Loessner
Summary: Pancreatic cancer is a devastating malignancy with limited treatment options. The tumor microenvironment, containing excessive extracellular matrix and stromal cells, acts as a barrier to drug delivery and contributes to therapy resistance and disease progression. To overcome this limitation, a multicellular 3D cancer model is developed to mimic key components of tumor tissues and assess responses to immunotherapy. Combining the CD11b agonist ADH-503 with anti-PD-1 immunotherapy and chemotherapy shows significant reduction in tumor cell viability, proliferation, metabolic activity, immunomodulation, and secretion of immunosuppressive and tumor growth-promoting cytokines.
ADVANCED HEALTHCARE MATERIALS
(2023)
Review
Nanoscience & Nanotechnology
Rodrigo Curvello, Verena Kast, Paloma Ordonez-Moran, Alvaro Mata, Daniela Loessner
Summary: Biology can be used to design materials and approaches for tumour tissue engineering, and biomaterials are necessary for rebuilding tissue organization, composition, and function. This review discusses bioengineering strategies that recreate the pathophysiology of tumour tissues to address questions in cancer research. Tissue engineering has produced innovative tools for cancer research.
NATURE REVIEWS MATERIALS
(2023)
Article
Pharmacology & Pharmacy
Tara Shabab, Onur Bas, Bronwin L. Dargaville, Akhilandeshwari Ravichandran, Phong A. Tran, Dietmar W. Hutmacher
Summary: This study combines melt-extrusion based 3D printing and porogen leaching to develop tunable multiphasic scaffolds for dental tissue regeneration. The scaffolds are highly adjustable in terms of mechanical properties, degradation kinetics, and surface morphology. The research also demonstrates improved cell attachment, proliferation, and extracellular matrix production on these multiscale scaffolds compared to single-scale counterparts, indicating their potential for enhanced tissue regeneration. Furthermore, the study explores the use of these scaffolds as drug delivery devices and shows that sustained drug release can be achieved. These findings strongly support further development of these scaffolds for dental tissue regeneration applications.
Article
Endocrinology & Metabolism
Marjorie Salga, Selwin G. Samuel, Hsu-Wen Tseng, Laure Gatin, Dorothee Girard, Bastien Rival, Valerie Barbier, Kavita Bisht, Svetlana Shatunova, Charlotte Debaud, Ingrid G. Winkler, Julie Paquereau, Aurelien Dinh, Guillaume Genet, Sebastien Kerever, Paer-Selim Abback, Sebastien Banzet, Francois Genet, Jean-Pierre Levesque, Kylie A. Alexander
Summary: Neurogenic heterotopic ossifications (NHO) are ectopic bones that develop in periarticular muscles after severe central nervous system (CNS) injuries. This study demonstrates that lipopolysaccharides (LPS) from gram-negative bacteria exacerbate NHO development in a toll-like receptor-4 (TLR4)-dependent manner, suggesting a functional association between gram-negative bacterial infections and NHO development. These findings highlight the importance of infection management in preventing NHO development in patients.
JOURNAL OF BONE AND MINERAL RESEARCH
(2023)
Article
Engineering, Manufacturing
Buddhi Herath, Markus Laubach, Sinduja Suresh, Beat Schmutz, J. Paige Little, Prasad K. D. V. Yarlagadda, Dietmar W. Hutmacher, Marie-Luise Wille
Summary: A modular design workflow was developed to facilitate the efficient design and manufacture of patient-specific scaffolds for scaffold-guided bone regeneration (SGBR). The workflow allows for semi-automatic filling of defect cavities, patient specificity, ideal surgical scaffold insertion, and generation of scaffolds based on various pore architectures. The real-time feedback from surgeons shortens the time required to finalize a patient-specific scaffold design.
VIRTUAL AND PHYSICAL PROTOTYPING
(2023)
Review
Engineering, Biomedical
Markus Laubach, Frank Hildebrand, Sinduja Suresh, Michael Wagels, Philipp Kobbe, Fabian Gilbert, Ulrich Kneser, Boris M. Holzapfel, Dietmar W. Hutmacher
Summary: The treatment of bone defects is a challenging clinical problem with high reintervention rates and significant healthcare costs. Three-dimensional printing has revolutionized the treatment of bone defects by enabling personalized therapies with highly porous 3D-printed implants. However, there are still challenges to be addressed, including the development of guidelines, clinical trial design, and financing support.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Biotechnology & Applied Microbiology
Mairim Russo Serafini, Alexandra Mowat, Susanah Mustafa, Siamak Saifzadeh, Tara Shabab, Onur Bas, Nicholas O'Rourke, Dietmar W. Hutmacher, Flavia Medeiros Savi
Summary: This study evaluated the use of mPCL scaffolds loaded with PRP for hernia repair in sheep models, and found that the mPCL scaffolds enhanced soft tissue regeneration. After 6 months, the mPCL scaffolds showed similar mechanical properties, collagen, and vascular ingrowth compared to PP meshes. This pilot study supports the use of mPCL as a safe and effective biodegradable scaffold for hernia and vaginal prolapse repair.
BIOENGINEERING-BASEL
(2023)
Meeting Abstract
Hematology
Allison R. Pettit, Lena Batoon, Ostyn Heng, David Hume, Kate Irvine, Simranpreet Kaur, Jean-Pierre Levesque, Graham Magor, Susan Millard, Jacqueline Noll, Andrew Perkins, Liza Raggatt, Cheyenne Sandrock, Anuj Sehgal, David Sester, Khatora Shanae Opperman, Kim Summers, Andy Wu, Andrew Zannettino
EXPERIMENTAL HEMATOLOGY
(2022)
Article
Orthopedics
Markus Laubach, Sinduja Suresh, Buddhi Herath, Marie-Luise Wille, Heide Delbrueck, Hatem Alabdulrahman, Dietmar W. Hutmacher, Frank Hildebrand
Summary: This study reports the successful application of patient-specific SGBR in the treatment of large complex long bone defects, highlighting the importance of scaffold design in promoting bone regeneration.
JOURNAL OF ORTHOPAEDIC TRANSLATION
(2022)
Article
Cell & Tissue Engineering
Hsu-Wen Tseng, Dorothee Girard, Kylie A. Alexander, Susan M. Millard, Frederic Torossian, Adrienne Anginot, Whitney Fleming, Jules Gueguen, Marie-Emmanuelle Goriot, Denis Clay, Beulah Jose, Bianca Nowlan, Allison R. Pettit, Marjorie Salga, Francois Genet, Marie-Caroline Le Bousse-Kerdiles, Sebastien Banzet, Jean-Pierre Levesque
Summary: Neurogenic heterotopic ossifications (NHOs) originate from fibroadipogenic progenitor cells (FAPs) in the injured muscle, rather than satellite cells (SCs). Spinal cord injury upregulates PDGFRα on FAPs, leading to the failure of SCs to regenerate muscle fibers in the injured muscle. These findings were validated using human NHO biopsies.
Article
Clinical Neurology
Kylie A. Alexander, Hsu-Wen Tseng, Irina Kulina, Whitney Fleming, Cedryck Vaquette, Francois Genet, Marc J. Ruitenberg, Jean-Pierre Levesque
Summary: This study found that functional T and B lymphocytes have minimal influence or dispensable contributions to the development of neurogenic heterotopic ossifications (NHO) after experimental spinal cord injury (SCI) in mice.
NEUROTRAUMA REPORTS
(2022)