Article
Agricultural Engineering
Yinling Gao, Fengqin Wang, Xiaojuan Li, Guotao Mao, Hui Xie, Andong Song, Julio Cesar dos Santos, Zhanying Zhang
Summary: This study investigated the production of citric acid and mannitol by Yarrowia lipolytica CGMCC 2.1506 using glucose and glycerol as carbon sources. Optimal cultivation conditions were determined and applied to enzyme hydrolysates derived from glycerol-assisted instant catapult steam explosion (ICSE) without detoxification, glycerol removal, or nutrient addition. High concentrations of citric acid and mannitol were achieved, demonstrating the potential of this integrated process for biorefining of lignocellulosic biomass.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Microbiology
Panagiota Diamantopoulou, Dimitris Sarris, Sidoine Sadjeu Tchakouteu, Evangelos Xenopoulos, Seraphim Papanikolaou
Summary: Sugar-rich waste streams are a significant source of environmental pollution, but they can be converted into valuable compounds through microbial fermentations. This study examined the potential of non-conventional yeast strains to produce single cell oil (SCO), microbial mass, and citric acid (CA) using expired glucose as a substrate. The results showed that some yeast strains were able to produce high quantities of SCO and CA, making them promising candidates for industrial applications.
Article
Biotechnology & Applied Microbiology
Bilge Sayin Borekci, Mukerrem Kaya, Guzin Kaban
Summary: Three Yarrowia lipolytica strains were screened for acid-production capacity, and the strain Y. lipolytica NRRL Y-1094 exhibited the highest production. Optimal conditions for citric acid production by Y. lipolytica NRRL Y-1094 were determined using response surface methodology, with the fermentation time having the greatest impact. This study lays the foundation for further optimization of citric acid production by Y. lipolytica NRRL Y-1094.
FERMENTATION-BASEL
(2022)
Article
Biochemistry & Molecular Biology
Veronika Urbanikova, Young-Kyoung Park, Daniela Krajciova, Mehdi Tachekort, Milan Certik, Ioana Grigoras, Roman Holic, Jean-Marc Nicaud, Peter Gajdos
Summary: In this study, the oleaginous yeast Yarrowia lipolytica was used as a host for the production of Punicic acid (PuA). By supplementing the medium with pomegranate seed oil, lipids containing PuA esters were accumulated up to 31.2% in Y. lipolytica. Strategies involving the expression of the bifunctional fatty acid conjugase/desaturase gene from Punica granatum (PgFADX) allowed for de novo production of PuA in Y. lipolytica. The optimized promoter for PgFADX expression resulted in a PuA production of 36.6 mg/L. These findings highlight the potential of Y. lipolytica as a promising host for PuA production.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Dimitris Sarris, Erminta Tsouko, Angelos Photiades, Sidoine Sadjeu Tchakouteu, Panagiota Diamantopoulou, Seraphim Papanikolaou
Summary: The global market for citric acid is expanding rapidly and its production using microbial bioprocessing can contribute to resource efficiency and low-carbon technologies. This study tested the potential of three wild-type Yarrowia lipolytica strains for citric acid production and found that one strain (ACA-YC 5033) showed the most promise. The strain was cultured on glucose-based media with partial replacement of fermentation water by olive-mill wastewaters, leading to higher citric acid production.
Article
Biotechnology & Applied Microbiology
Erdem Carsanba, Bilal Agirman, Seraphim Papanikolaou, Patrick Fickers, Huseyin Erten
Summary: The increase in bread wastage leads to ecological problems like methane and CO2 production. To reduce this, a sustainable system of waste bread management should be established. Waste bread has a high potential to be used as feedstock for the production of value-added bio products.
FERMENTATION-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Monireh Marsafari, Fidelis Azi, Shaohua Dou, Peng Xu
Summary: By manipulating subcellular localization and metabolic flux in a co-culture system of microbes, the production of Amorphadiene, a precursor to the antimalarial drug artemisinin, can be significantly improved, providing an alternative method for its synthesis.
MICROBIAL CELL FACTORIES
(2022)
Article
Biotechnology & Applied Microbiology
Patricia Ferreira, Marlene Lopes, Isabel Belo
Summary: This study investigates the use of unconventional bioreactors, including a pressurized stirred tank reactor and an airlift bioreactor, to improve the oxygen mass transfer rate (OTR) and increase citric acid production. The results show that both bioreactor types can successfully enhance OTR and achieve higher citric acid yields, providing cost-saving benefits.
FERMENTATION-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Laura Mitrea, Lavinia-Florina Calinoiu, Bernadette-Emoke Teleky, Katalin Szabo, Adrian-Gheorghe Martau, Bianca -Eugenia Stefanescu, Francisc-Vasile Dulf, Dan-Cristian Vodnar
Summary: This study investigated the ability of Y. lipolytica to biosynthesize succinic acid and citric acid using different carbon sources. The results showed that the highest colony-forming units, cell dry weight, citric acid, and succinic acid production were obtained in cultivations performed on waste cooking oil. These findings highlight the industrial potential of Y. lipolytica in bioconverting lipidic residual biomass with negative environmental implications into valuable organic compounds.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2022)
Review
Biotechnology & Applied Microbiology
Young-Kyoung Park, Rodrigo Ledesma-Amaro
Summary: Yarrowia lipolytica is a promising candidate for industrial bioproduction of chemicals, fuels, foods, and pharmaceuticals, thanks to its natural and engineered traits. In recent years, extensive research has been conducted to assess its potential, improve its features, and scale up its processes. The increasing number of publications and patents related to Y. lipolytica demonstrates its importance and potential in the field. Despite the remaining challenges, it is expected that Y. lipolytica will become one of the preferred choices for industrial applications in the near future.
TRENDS IN BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Zedi Wang, Linlin Zhou, Minrui Lu, Yuwei Zhang, Samina Perveen, Huarong Zhou, Zhiqiang Wen, Zhaoxian Xu, Mingjie Jin
Summary: Yarrowia lipolytica strain was evolved through adaptive laboratory evolution to increase tolerance to ferulic acid, leading to a new strain with improved viability under high concentrations of ferulic acid, showing promise for application in lignocellulose conversion.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Peter Gajdos, Veronika Urbanikova, Maria Vicenova, Milan Certik
Summary: Yarrowia lipolytica has been genetically modified to enhance the production of very long chain fatty acids (VLCFA), such as behenic acid and erucic acid, which are industrially valuable compounds. By overexpressing specific enzymes and blocking fatty acid degradation pathways, the production of VLCFA in Y. lipolytica has been significantly improved.
MICROBIAL CELL FACTORIES
(2022)
Article
Biochemistry & Molecular Biology
Svetlana Kamzolova, Julia N. Lunina, Vladimir A. Samoilenko, Igor G. Morgunov
Summary: Yarrowia lipolytica yeast is capable of synthesizing citric acid, and the concentration of ammonium sulfate significantly affects its growth and citric acid production.
Article
Biochemistry & Molecular Biology
Piotr Juszczyk, Anita Rywinska, Julia Kosicka, Ludwika Tomaszewska-Hetman, Waldemar Rymowicz
Summary: This study aimed to screen Yarrowia lipolytica yeast strains for the efficient and selective biosynthesis of mannitol from glycerol. The results showed that strain S3 had the highest mannitol selectivity at lower glycerol concentrations, and a fed-batch culture system increased mannitol production.
Article
Biotechnology & Applied Microbiology
Lanxin Rong, Lin Miao, Shuhui Wang, Yaping Wang, Shiqi Liu, Zhihui Lu, Baixiang Zhao, Cuiying Zhang, Dongguang Xiao, Krithi Pushpanathan, Adison Wong, Aiqun Yu
Summary: This study developed a microbial cell factory that utilizes waste cooking oil as raw material for the production of itaconic acid. Through gene expression and overexpression, the production yield was significantly improved, achieving a high IA titer in a 5 L bioreactor.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Environmental Sciences
Senba Komesli, Sumeyya Akbulut, Nazli Pinar Arslan, Ahmet Adiguzel, Mesut Taskin
Summary: This study successfully utilized locally isolated cold-adapted bacteria under non-sterile culture conditions to produce lipase and hydrolyze waste frying oils. The most favorable conditions were found to be low temperature, restricted nutrient availability and high inoculum volume, which effectively prevented microbial contaminants.
ENVIRONMENTAL TECHNOLOGY
(2021)
Article
Environmental Sciences
Nazli Pinar Arslan, Mehmet Nuri Aydogan
Summary: This study investigated the feasibility of using sheep wool peptone (SWP) and molasses as substrates for hyaluronic acid (HA) production, finding that SWP-VI was the most favorable for HA production and increasing cell biomass (CB) yield. Commercial protease peptone (PP) was found to be more suitable for lactic acid (LA) production compared to SWP-VI.
WASTE AND BIOMASS VALORIZATION
(2021)
Article
Biotechnology & Applied Microbiology
Yaprak Ilayda Karadayi, Gurkan Aykutoglu, Nazli Pinar Arslan, Mustafa Ozkan Baltaci, Ahmet Adiguzel, Mesut Taskin
Summary: This study revealed the EPS production potential of A. gonensis and the anticancer activity of the produced EPS. It was shown for the first time that the main monomer of bacterial heteropolysaccharides would be xylose.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2021)
Article
Microbiology
Berna Genc, Mesut Taskin, Ahmet Adiguzel
Summary: The study investigated the biological, rheological, and chemical properties of EPS purified from the thermophilic bacterium Anoxybacillus pushchinoensis G11. The EPS showed antibiofilm and antitumor activities, exhibited a heteropolysaccharide composition, and had a molecular weight of 75.5 kDa.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Biochemical Research Methods
Aysenur Yazici, Serkan Ortucu, Mesut Taskin
Summary: This study isolated, identified, and extracted the antifungal polypeptide astucin produced by the fungus A. tubingensis A01, which was found to have antimicrobial and antibiofilm activity against Staphylococcus aureus.
JOURNAL OF PROTEOMICS
(2021)
Article
Biotechnology & Applied Microbiology
Elanur Tuysuz, Hakan Ozkan, Nazli P. Arslan, Ahmet Adiguzel, Mustafa O. Baltaci, Mesut Taskin
Summary: A protein hydrolysate from sheep wool was successfully prepared using the locally isolated keratinolytic bacterium Bacillus licheniformis EY2, and converted to wool peptone WP, which showed lower production cost and superior cell growth performances compared to commercial peptones. This study presents a environmentally friendly approach for reducing waste sheep wool while providing a cost-effective alternative for microbial cultivation.
BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR
(2021)
Article
Biochemistry & Molecular Biology
Nazli Pinar Arslan, Osman Nuri Keles, Nurdan Gonul-Baltaci
Summary: This study found that both Ag NPs and TiO2 NPs could enter the mouse testis through the blood-testis barrier and accumulate in the tissue, but Ag NPs did not significantly impact mitochondrial dynamics in testicular cells, while TiO2 NPs resulted in decreased sperm motility and increased mitochondrial fission.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2022)
Article
Energy & Fuels
Nazli Pinar Arslan, Handan Cinar-Yilmaz, Dilek Vural-Keles, Meryem Doymus, Ferah Yilmaz, Mesut Taskin
Summary: This study aimed to produce exopolysaccharide (EPS) and mycelial biomass from the edible mushroom Lentinus edodes using peptone prepared from waste sheep wool. Wool peptones were found to be richer in minerals, especially Na and Cl, and more favorable for EPS synthesis compared to commercial peptones. However, commercial peptones resulted in relatively higher mycelial biomass concentrations.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Microbiology
Muhammed Enes Kiziler, Tugba Orak, Meryem Doymus, Nazli Pinar Arslan, Ahmet Adiguzel, Mesut Taskin
Summary: This study investigates the effects of fungal quorum sensing molecules (tyrosol and farnesol) on carotenoid synthesis in the yeast Rhodotorula glutinis and prodigioin synthesis in the bacterium Serratia marcencens. The results show that tyrosol supplementation increases carotenoid synthesis, while farnesol supplementation increases prodigiosin synthesis. The addition of these molecules on day 3 compared to day 1 leads to a greater increase in pigment synthesis. The findings suggest the potential of using farnesol and tyrosol as novel inducers for large-scale production of microbial pigments.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Engineering, Environmental
Fatih Kayar, Meryem Doymus, Hakan Ozkan, Nazli Pinar Arslan, Ahmet Adiguzel, Mesut Taskin
Summary: In this study, chitinous material was produced from locally isolated Penicillium crustosum using molasses as substrate. Chitosan was prepared from the chitinous material and its antibacterial activity was investigated. The chitosan showed higher antibacterial efficiency compared to commercial chitosan, making it potentially useful for biomedical applications. This study provides important insights into the production and antibacterial properties of chitosan.
JOURNAL OF POLYMERS AND THE ENVIRONMENT
(2022)
Article
Biotechnology & Applied Microbiology
Mehmet Akif Omeroglu, Nurdan Gonul-Baltaci, Nazli Pinar Arslan, Ahmet Adiguzel, Mesut Taskin
Summary: This study investigates the microbial conversion of waste baklava syrup (WBS) into biofuels and bioproducts. The results show that useful compounds such as citric acid, levan, and lipids can be produced through microbial fermentation at optimal concentrations of WBS. This study is the first to report the use of WBS as a substrate in microbial studies.
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
(2022)
Article
Energy & Fuels
Fatma Altinok, Seyda Albayrak, Nazli Pinar Arslan, Mesut Taskin, Esra Aygun, Melda Sisecioglu, Ahmet Adiguzel
Summary: The lipase of Anoxybacillus gonensis UF7 was successfully purified and characterized in this study. It exhibited high activity and stability at pH 8.0 and 60 degrees C. The purified lipase was also found to be effective as a catalyst for biodiesel production, with a transesterification yield of 87.7%.
Article
Biotechnology & Applied Microbiology
Mehmet Akif Omeroglu, Seyda Albayrak, Nazli Pinar Arslan, Hakan Ozkan, Ahmet Adiguzel, Mesut Taskin
Summary: The present study tested the usability of low-cost sheep wool peptone (SWP) as an organic nitrogen source in the production of six industrially important enzymes. SWP was found to be superior to commercial tryptone peptone (TP) in the production of four enzymes. This study is the first to investigate the usage of sheep wool protein hydrolysate (SWP) as an organic nitrogen source or a peptone in the fermentative production of microbial enzymes.
