Journal
JOURNAL OF BIOTECHNOLOGY
Volume 228, Issue -, Pages 58-66Publisher
ELSEVIER
DOI: 10.1016/j.jbiotec.2016.04.027
Keywords
Spores; Adsorption; Biolistic; Gene gun; DNA vaccine
Categories
Funding
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2011/13805-1]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [11/13805-1] Funding Source: FAPESP
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Bacillus subtilis spores represent a suitable platform for the adsorption of proteins, enzymes and viral particles at physiological conditions. In the present work, we demonstrate that purified spores can also adsorb DNA on their surface after treatment with cationic molecules. In addition, we demonstrate that DNA coated B. subtilis spores can be used as particulate carriers and act as an alternative to gold microparticles for the biolistic (gene gun) administration of plasmid DNA in mice. Gene gun delivery of spores pre-treated with DODAB (dioctadecyldimethylammonium bromide) allowed efficient plasmid DNA absorption and induced protein expression levels similar to those obtained with gold microparticles. More importantly, we demonstrated that a DNA vaccine adsorbed on spores can be loaded into biolistic cartridges and efficiently delivered into mice, which induced specific cellular and antibody responses. Altogether, these data indicate that B. subtilis spores represent a simple and low cost alternative for the in vivo delivery of DNA vaccines by the gene gun technology. (C) 2016 Elsevier B.V. All rights reserved.
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