Fabrication of a novel nano-biosensor for efficient colorimetric determination of uric acid

In this study, a novel nano-biosensor for uric acid detection has been designed and fabricated by embedding uricase into a copper nanozyme, which possessed outstanding peroxidase-like activity and could efficiently mimic horseradish peroxidase (HRP). After optimizing the preparation conditions and characterization, we successfully applied the nano-biosensor to detect uric acid levels. The equation of linear regression was prescribed as y = 0.0064x + 0.3299, while its correlation coefficient is 0.9886. The colorimetric sensor exhibited a linear response range over 1-50 mu M and a relatively low limit of detection (LOD) of 0.6 mu M. The nano-biosensor has the advantages of easy preparation, good reusability and excellent storage stability, while this one-step colorimetric method is simple to be operated and has high selectivity for detection of the uric acid levels in actual human samples.

Automated summary

In this study, a novel nano-biosensor for uric acid detection has been designed and fabricated by embedding uricase into a copper nanozyme, which possesses outstanding peroxidase-like activity. The nano-biosensor has easy preparation, good reusability, excellent storage stability, and shows high selectivity for detection of uric acid levels in actual human samples.