Article
Biochemistry & Molecular Biology
Luckika Panthiya, Jiraporn Tocharus, Amnart Onsa-ard, Waraluck Chaichompoo, Apichart Suksamrarn, Chainarong Tocharus
Summary: The study found that HHC can improve vascular function and attenuate vascular remodeling in hypertensive rats. Its potential mechanisms of action may involve antioxidant and anti-inflammatory effects.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2022)
Article
Pharmacology & Pharmacy
Andre Felipe Rodrigues, Mihail Todiras, Fatimunnisa Qadri, Natalia Alenina, Michael Bader
Summary: The study found that FVB/N mice without angiotensin peptides do not experience hypotension but regulate blood pressure through compensatory mechanisms. However, these mice also exhibit renal morphological and physiological changes, which may make them a model for drug-resistant hypertension.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Article
Multidisciplinary Sciences
Nuria Benseny-Cases, Elena Alvarez-Marimon, Ester Aso, Margarita Carmona, Oxana Klementieva, Dietmar Appelhans, Isidre Ferrer, Josep Cladera
Summary: This study used infrared imaging to identify early-stage amyloid aggregates in the brains of APP/PS1 transgenic mice for the first time, showing a potential therapeutic effect by decreasing these aggregates. The non-fibrillary species identified may serve as potential pharmacological targets for Alzheimer's disease, and early detection of these structures could be useful for non-invasive diagnostic techniques.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Pablo Nakagawa, Javier Gomez, Ko-Ting Lu, Justin L. Grobe, Curt D. Sigmund
Summary: High sodium intake is associated with increased risk of cardiovascular diseases, while deficiency in renin-b may lead to higher susceptibility to salt-induced hypertension.
Article
Biochemistry & Molecular Biology
Angus Lindsay, Adam J. Trewin, Kate J. Sadler, Claire Laird, Paul A. Della Gatta, Aaron P. Russell
Summary: The study found that stress-resistant mdx mice show higher activity levels and better physiological function in response to behavioral stress, including greater exercise and muscle oxidative capacity, as well as less severe muscle histopathology. Female mdx mice exhibit higher activity levels following behavioral stress compared to males, a response that can be eliminated after ovariectomy but restored with estradiol.
Article
Biochemistry & Molecular Biology
Andrea Zemba Cilic, Mladen Zemba, Matija Cilic, Sanja Strbe, Spomenko Ilic, Jaksa Vukojevic, Zoran Zoricic, Igor Filipcic, Antonio Kokot, Ivan Maria Smoday, Iva Rukavina, Alenka Boban Blagaic, Ante Tvrdeic, Bozidar Duplancic, Vasilije Stambolija, Darko Marcinko, Anita Skrtic, Sven Seiwerth, Predrag Sikiric
Summary: In this study, we used BPC 157 and NO agents to counteract ketamine-induced negative-like schizophrenia symptoms. BPC 157 was found to alleviate cognitive dysfunction, social withdrawal, and anhedonia, and also had an additional anxiolytic effect. L-NAME and L-arginine had different effects on different symptoms. The gene expression studies in brain tissue showed that BPC 157 had a specific effect on ketamine-specific targets.
Article
Pharmacology & Pharmacy
Ayoub Amssayef, Mohammed Ajebli, Mohamed Eddouks
Summary: Lichens, a symbiotic combination of a fungus and a green alga or cyanobacterium, have long been used in traditional medicine for their effectiveness against skin disorders, diabetes, and hypertension. This study revealed that the aqueous extract of Oakmoss has antihypertensive and vasorelaxant properties, showing significant effects in hypertensive rats.
CLINICAL AND EXPERIMENTAL HYPERTENSION
(2021)
Article
Multidisciplinary Sciences
Valerio Licursi, Wei Wang, Elena Di Nisio, Francesco P. Cammarata, Rosaria Acquaviva, Giorgio Russo, Lorenzo Manti, Mariangela Cestelli Guidi, Emiliano Fratini, Gihan Kamel, Roberto Amendola, Pietro Pisciotta, Rodolfo Negri
Summary: The study investigates the potential impact and diffusion extent of proton beam therapy on surrounding tissues, using transcriptome analysis of biomarker genes and assessment of lipid alterations. Evidence of transcriptional modulation at considerable distance from the beam's target is shown, indicating a possible diffusion of regulatory molecules from high dose irradiated cells to distant tissues.
JOURNAL OF RADIATION RESEARCH AND APPLIED SCIENCES
(2021)
Article
Physiology
Sofie De Moudt, Jhana O. Hendrickx, Cedric Neutel, Dorien De Munck, Arthur Leloup, Guido R. Y. De Meyer, Wim Martinet, Paul Fransen
Summary: This study investigated the longitudinal changes in aortic reactivity and biomechanics in L-NAME treated mice. The results showed that arterial stiffness preceded peripheral hypertension and cardiac hypertrophy, and the underlying mechanisms shifted from early endothelial dysfunction to late-term vascular smooth muscle cell dysfunction.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Anatomy & Morphology
Julia Sophie Ehlers, Katharina Bracke, Viola von Bohlen Und Halbach, Florian Siegerist, Nicole Endlich, Olivervon Bohlen Und Halbach
Summary: The SLC35F1 gene mutation in humans is associated with neurodevelopmental disorders. However, Slc35f1-deficient mice do not exhibit obvious deficits in learning and memory, and neuroanatomical parameters of neuronal plasticity are also unaffected. Therefore, they do not display a neurodevelopmental phenotype.
BRAIN STRUCTURE & FUNCTION
(2023)
Article
Behavioral Sciences
Andrea Zemba Cilic, Mladen Zemba, Matija Cilic, Igor Balenovic, Sanja Strbe, Spomenko Ilic, Jaksa Vukojevic, Zoran Zoricic, Igor Filipcic, Antonio Kokot, Domagoj Drmic, Alenka Boban Blagaic, Ante Tvrdeic, Sven Seiwerth, Predrag Sikiric
Summary: In the rat models, BPC 157 and L-arginine counteracted schizophrenia-like symptoms, while L-NAME was ineffective in some cases. Further studies are needed to determine the precise relationships between BPC 157, dopamine, glutamate, and the NO system for potential clinical applications.
BEHAVIOURAL BRAIN RESEARCH
(2021)
Article
Food Science & Technology
Lin Yuan, Ying Li, Moutong Chen, Liang Xue, Juan Wang, Yu Ding, Jumei Zhang, Shi Wu, Qinghua Ye, Shuhong Zhang, Runshi Yang, Hui Zhao, Lei Wu, Tingting Liang, Xinqiang Xie, Qingping Wu
Summary: The intervention of PFM-SR37-3 and PFM-SR61-2 can lower blood pressure and attenuate renal injury in hypertensive rats. This effect may be achieved by reshaping the gut microbiota and metabolome, regulating specific metabolites in the intestine and blood circulation, and modulating the BP circadian rhythm pathway.
Article
Medicine, Research & Experimental
Soon Y. Tang, Hu Meng, Sean T. Anderson, Dimitra Sarantopoulou, Soumita Ghosh, Nicholas F. Lahens, Katherine N. Theken, Emanuela Ricciotti, Elizabeth J. Hennessy, Vincent Tu, Kyle Bittinger, Aalim M. Weiljie, Gregory R. Grant, Garret A. FitzGerald
Summary: Inhibitors of microsomal prostaglandin E synthase 1 (mPGES-1) show potential in clinical development, but may lead to increased blood pressure response to salt loading in male mice, with the compensatory role of atrial natriuretic peptide (ANP) restrained hypertension. Conversely, female mice do not exhibit this hypertensive response, with estrogen compensating for the deficiency in prostacyclin (PGI2). This suggests a potential risk of hypertensive response to mPGES-1 inhibitors in men with hyperlipidemia on a high-salt diet.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Multidisciplinary Sciences
Marit Jensen, Christian Mueller, Norbert Huebner, Giannino Patone, Kathrin Saar, Chi-Un Choe, Edzard Schwedhelm, Tanja Zeller
Summary: The deficiency of AGAT and the supplementation of creatine and hArg are associated with cardiac miRNA expression, which may influence cardiac (dys)function and cardiovascular disease (CVD).
SCIENTIFIC REPORTS
(2022)
Article
Mathematics, Applied
O. K. Gasymov, A. H. Aydemirova, L. A. Melikova, J. A. Aliyev
Summary: Early diagnosis is crucial for the survival of lung cancer patients, yet effective screening techniques are lacking. Artificial Intelligence was utilized to predict human lung carcinoma based on a biomarker model, showing promise in developing a fast, minimally invasive and cost-effective screening method.
APPLIED AND COMPUTATIONAL MATHEMATICS
(2021)
Article
Instruments & Instrumentation
Karolina Augustyniak, Karolina Chrabaszcz, Marta Smeda, Marta Stojak, Katarzyna M. Marzec, Kamilla Malek
Summary: Label-free molecular imaging, particularly with higher magnification in FT-IR microscopy, can provide detailed insights into lung structures and histopathological features, allowing for better evaluation of chemical composition in disease models.
APPLIED SPECTROSCOPY
(2022)
Article
Biochemistry & Molecular Biology
Aleksandra Borek-Dorosz, Marek Grosicki, Jakub Dybas, Ewelina Matuszyk, Marko Rodewald, Tobias Meyer-Zedler, Michael Schmitt, Juergen Popp, Kamilla Malek, Malgorzata Baranska
Summary: The study compared the response to inflammation between isolated human eosinophils and EoL-1 cells in terms of lipid bodies (LBs) using fluorescence, Raman, and coherent anti-Stokes Raman scattering (CARS) microscopy. Differences were observed in the number and chemical composition of LBs, indicating varying manifestations of inflammatory processes depending on the cell type and stimulation.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Chemistry, Analytical
Ewelina Wiercigroch, Pawel Swit, Agnieszka Brzozka, Lukasz Pieta, Kamilla Malek
Summary: In this study, a novel SERS immunoassay is demonstrated, operating in a dual surface enhancement and dual-tag paradigm, which improves the detection sensitivity and reliability.
Article
Spectroscopy
Prasad L. Polavarapu, Jordan L. Johnson, Vijay Raghavan, Grzegorz Zajac, Malgorzata Baranska
Summary: Experimental vibrational Raman and Raman optical activity (ROA) spectra were measured for diacetyl L-tartaric acid (DAT), its esters T1OH and T12OH, and corresponding sodium salts DATNa, T1ONa, and T12ONa. Quantum chemical (QC) predictions using the B3LYP functional and 6-311++G(2d,2p) basis set were used to interpret the ROA spectra. Implicit solvation model (PCM) was inadequate for predicting the ROA spectra of DAT and T1OH in aqueous solutions. However, it satisfactorily predicted the ROA spectra for DATNa and T1ONa. The overwhelming number of conformations for T12OH and T12ONa hindered QC predictions, but predictions made for T1OH and T1ONa can be applied to explain the experimental ROA spectra of T12OH and T12ONa. The study highlights the importance of converting carboxylic acids to sodium salts and QC predictions for shorter alkyl chain analogs in interpreting the ROA spectra of chiral surfactants.
JOURNAL OF RAMAN SPECTROSCOPY
(2022)
Article
Chemistry, Multidisciplinary
Monika Soltys-Mroz, Karolina Syrek, Lukasz Pieta, Kamilla Malek, Grzegorz D. Sulka
Summary: In this study, Fe2O3-TiO2 materials were synthesized by cathodic electrochemical deposition, and the effect of hematite content on the photoelectrochemical activity of the materials was investigated. The results showed that the amount of deposited iron and hematite content increased with deposition time, directly impacting the semiconducting properties and enhancing the photoelectrochemical water oxidation and visible light response of the materials.
Article
Biophysics
Basseem Radwan, Stefano Rocchetti, Ewelina Matuszyk, Magdalena Sternak, Maciej Stodulski, Robert Pawlowski, Jacek Mlynarski, Krzysztof Brzozowski, Stefan Chlopicki, Malgorzata Baranska
Summary: This study introduces a Raman-based imaging method using EdU as a probe to track endothelial cell proliferation, providing an alternative to fluorescence-based assays for assessing endothelial proliferation and regeneration.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Monika Kujdowicz, David Perez-Guaita, Piotr Chlosta, Krzysztof Okon, Kamilla Malek
Summary: A novel approach combining infrared microscopy and machine learning was proposed to automatically predict the presence of cancer, invasiveness, and grade in urothelial bladder carcinoma (BC). The study found that the infrared imaging showed high sensitivity in identifying cancer, invasiveness, and grade, providing a potential ancillary platform for screening bladder biopsies.
Article
Biochemistry & Molecular Biology
Aleksandra Borek-Dorosz, Anna Pieczara, Krzysztof Czamara, Marta Stojak, Ewelina Matuszyk, Katarzyna Majzner, Krzysztof Brzozowski, Arianna Bresci, Dario Polli, Malgorzata Baranska
Summary: In this study, the functionality of inflamed cells was verified by analyzing their ability to uptake and accumulate exogenous saturated fatty acids. The results showed that the uptake of fatty acids in inflamed cells is dependent on their concentration and type, providing a better understanding of endothelial cell inflammation and its impact on their fat buffering capacity. The microscopic methods used in the analysis showed complementarity in studying fatty acid uptake by endothelial cells from different perspectives.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biophysics
Anna Pieczara, Aleksandra Borek-Dorosz, Szymon Buda, William Tipping, Duncan Graham, Robert Pawlowski, Jacek Mlynarski, Malgorzata Baranska
Summary: This study uses a Raman probe, 3-OPG, to track metabolic changes in endothelial cells. The results show that 3-OPG is a sensitive sensor for studying glucose metabolism and its changes in cellular inflammation. This research is important for understanding the role of endothelial cell metabolism in disease development.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Biochemistry & Molecular Biology
Karolina Augustyniak, Aleksandra Pragnaca, Monika Lesniak, Marta Halasa, Agata Borkowska, Ewa Pieta, Wojciech M. Kwiatek, Claudine Kieda, Robert Zdanowski, Kamilla Malek
Summary: Circulating endothelial cell progenitors from the bone marrow have potential in repairing endothelium damage. This study used label-free Raman and FTIR imaging to distinguish between brain endothelium and endothelial progenitor cells in mice. The results showed that lipid droplets play a key role in differentiating these two cell types.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Spectroscopy
Vikas Kumar, Till Reichenauer, Luca Supovec, Dennis Jansen, Nikolai Brodt, Grzegorz Zajac, Agnieszka Domagala, Malgorzata Baranska, Jochen Niemeyer, Sebastian Schluecker
Summary: CARS-ROA is a coherent technique for chirally sensitive vibrational spectroscopy, which has the advantage of significantly reducing acquisition times compared to traditional ROA. In this study, the first demonstration of heterodyne-detected CARS-ROA of chiral molecules in an achiral solvent was achieved using a conventional broadband CARS spectrometer. This work is a crucial step towards kinetic reaction monitoring in organocatalysis with short acquisition times.
JOURNAL OF RAMAN SPECTROSCOPY
(2023)
Article
Chemistry, Analytical
Basseem Radwan, Amrutha Prabhakaran, Stefano Rocchetti, Ewelina Matuszyk, Tia E. Keyes, Malgorzata Baranska
Summary: The cellular uptake and bioavailability of anti-inflammatory antioxidant astaxanthin (AXT) can be improved via liposomal encapsulation. Endothelial cells (EC), which line blood vessels, play important roles in maintaining cardiovascular homeostasis. Dysfunction of EC is associated with various diseases and is closely related to oxidative stress and inflammation. Liposomal encapsulation of AXT showed enhanced uptake and anti-inflammatory effects, especially in reducing lipid unsaturation, lipid droplets, and intercellular adhesion molecule 1 (ICAM-1) overexpression in EC.
Review
Chemistry, Analytical
Adriana Adamczyk, Sylwia Orzechowska, Anna Maria Nowakowska, Krzysztof Brzozowski, Katarzyna Majzner, Malgorzata Baranska
Summary: This article presents the recent advances in using stimulated Raman scattering (SRS) microscopy for spectroscopic analysis of cancer cells to identify pathological changes and understand the mechanism of metastasis. SRS microscopy, with its stronger signals and ability to time-resolve vibrational motions, offers rapid analysis of single cells. The article showcases the development of modern Raman imaging technology based on nonlinear optics and its application in analyzing various tumor cells, particularly leukemia.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Ewa Machalska, Grzegorz Zajac, Malgorzata Baranska, Petr Bour, Dorota Kaczorek, Robert Kawecki, Joanna E. Rode, Krzysztof Lyczko, Jan Cz Dobrowolski
Summary: In this study, we discovered that the recently discovered ECD-Raman effect is dominant over natural Raman optical activity in atropisomeric naphthalenediimides. We investigated the type of molecular structure information that can be obtained from the spectra. We found that the spectra depended significantly on solute substitution and/or solvent change. Furthermore, the spectra could be accurately predicted by theory, providing an interesting tool for monitoring the chirality of binaphthyl compounds.
CHEMICAL COMMUNICATIONS
(2022)