Article
Immunology
Marion Le Rochais, Patrice Hemon, Danivanh Ben-guigui, Soizic Garaud, Christelle Le Dantec, Jacques-Olivier Pers, Divi Cornec, Arnaud Uguen
Summary: Persistent inflammation can lead to the development of tertiary lymphoid structures (TLS) resembling secondary lymphoid organs (SLO). Comparing TLS to SLO in digestive tract cancers and inflammatory bowel diseases revealed differences in their structure and functionality. TLS followed a maturation spectrum with distinct stages, and the architectural and functional grading of TLS showed variation across diseases.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Immunology
Yael Glasson, Laure-Agnes Chepeaux, Anne-Sophie Dume, Virginie Lafont, Julien Faget, Nathalie Bonnefoy, Henri-Alexandre Michaud
Summary: Solid tumors have a dynamic ecosystem with various cell types constantly interacting, and their abundance and functional orientation change over time and in response to treatment. High-dimensional imaging mass cytometry (IMC) has emerged as a powerful tool to explore the complexity of tumors at the single-cell level. This review summarizes the potential of IMC in cancer research, from preclinical to clinical studies.
SEMINARS IN IMMUNOPATHOLOGY
(2023)
Article
Biochemical Research Methods
Eric Lee, Kevin Chern, Michael Nissen, Xuehai Wang, I. M. A. X. T. Consortium IMAXT Consortium, Chris Huang, Anita K. Gandhi, Alexandre Bouchard-Cote, Andrew P. Weng, Andrew Roth
Summary: Recent advances in spatial proteomics technologies have allowed for the profiling of multiple proteins in single cells, creating the opportunity to explore spatial relationships between cells. However, current clustering methods do not consider spatial context or prior knowledge about cell populations. In response, the authors developed SpatialSort, a Bayesian clustering approach that incorporates spatial awareness and prior biological knowledge to improve clustering accuracy and perform automated annotation.
Article
Biochemical Research Methods
Sindhura Thirumal, Amoon Jamzad, Tiziana Cotechini, Charles T. Hindmarch, Charles H. Graham, D. Robert Siemens, Parvin Mousavi
Summary: This article presents an open-source software called TITAN for processing and analyzing Imaging Mass Cytometry (IMC) data. TITAN provides a single environment for image visualization, segmentation, analysis, and export of IMC data, making it more user-friendly compared to current methods.
Article
Medicine, General & Internal
Brian Healey Bird, Ken Nally, Karine Ronan, Gerard Clarke, Sylvie Amu, Ana S. Almeida, Richard Flavin, Stephen Finn
Summary: Immune checkpoint inhibitors have changed the way cancer is treated but their effectiveness is limited and they can cause autoimmune side effects. Existing biomarkers are not sensitive or specific. This review discusses emerging tumor and immune tissue biomarkers and the impact of the gut microbiome on treatment outcomes.
Article
Immunology
Roxane Elaldi, Patrice Hemon, Luciana Petti, Estelle Cosson, Belinda Desrues, Anne Sudaka, Gilles Poissonnet, Ellen Van Obberghen-Schilling, Jacques-Olivier Pers, Veronique M. Braud, Fabienne Anjuere, Aida Meghraoui-Kheddar
Summary: The IMC panel with 39 antibodies designed for staining human tumor sections provides a powerful tool for studying tumor immune microenvironment, revealing interactions between immune cells and structural components such as tumor cells.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Oncology
Chao Feng, Xi Wang, Yuting Tao, Yuanliang Xie, Zhiyong Lai, Zhijian Li, Jiaxin Hu, Shaomei Tang, Lixin Pan, Liangyu He, Qiuyan Wang, Tianyu Li, Zengnan Mo
Summary: Muscle invasive bladder cancer (MIBC) is a malignancy with significant heterogeneity. The MIBC tumor microenvironment (TME) is highly complex, comprising diverse phenotypes and spatial architectures. This study used a combination of mass cytometry and imaging mass cytometry to analyze the complexity of the MIBC TME at the single-cell level. The identification of abnormal cell clusters and spatial characteristics provides valuable insights for potential targeted therapies in MIBC patients.
Review
Medical Laboratory Technology
Pey Yee Lee, Yeelon Yeoh, Nursyazwani Omar, Yuh-Fen Pung, Lay Cheng Lim, Teck Yew Low
Summary: MALDI imaging is a powerful technology that allows for global molecular mapping of biomolecules in tissues without the need for labeling. It has shown great potential in probing tumor heterogeneity and cancer-related molecular events, providing unique insights into molecular content and spatial details.
CRITICAL REVIEWS IN CLINICAL LABORATORY SCIENCES
(2021)
Article
Chemistry, Analytical
Hua Tian, Louis J. Sparvero, Tamil Selvan Anthonymuthu, Wan-Yang Sun, Andrew A. Amoscato, Rong-Rong He, Hulya Bayr, Valerian E. Kagan, Nicholas Winograd
Summary: This study developed a new methodology for comprehensive lipidomic and metabolomic profiling of individual cells on frozen-hydrated tissue sections, combined with cell-type specific lanthanide antibodies analysis. The research revealed distinct variations of distribution and intensities of key ions in different types of cells within the tumor microenvironment. The feasibility of employing SIMS imaging for multi-omics profiling at the single-cell level in the same tissue section opens up possibilities for gaining new insights into cellular interactions in various tissue microenvironments.
ANALYTICAL CHEMISTRY
(2021)
Article
Immunology
Melissa A. Meyer, Huy Q. Dinh, Ahmad Alimadadi, Daniel J. Araujo, Nandini Chatterjee, Norma A. Gutierrez, Yanfang Peipei Zhu, Emma L. Hunter, Shu Liang, Gregory Seumois, William B. Kiosses, Sergio D. Catz, Pandurangan Vijayanand, Christian Ottensmeier, Catherine C. Hedrick
Summary: Recent research has found that neutrophils in melanoma patients are composed of unique subsets with specific functions. Using mass cytometry, this study identified CD79b(+) neutrophils that are present in the blood of early-stage melanoma patients and in tumors of head and neck cancer patients. Machine learning analysis showed that CD79b expression in peripheral blood neutrophils is important in identifying melanoma incidence. CD79b(+) neutrophils have characteristics of neutrophils but display transcriptional and surface-marker phenotypes reminiscent of B cells, and they are primed for NETosis, express higher levels of antigen presentation-related proteins, and have an increased capacity for phagocytosis.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Oncology
Simon Gray, Christian H. Ottensmeier
Summary: NSCLC remains a significant cause of morbidity and mortality, and the effectiveness of ICIs treatment is limited for most patients. The use of MAB technologies to analyze TME can provide insights into treatment susceptibility, resistance, and prognosis factors, and discover new therapeutic approaches.
Review
Immunology
Marco Antonio Fonseca-Montano, Karla Itzel Vazquez-Santillan, Alfredo Hidalgo-Miranda
Summary: Breast cancer is a common and deadly malignancy in women, with alterations in genes and signaling pathways playing a significant role. Abnormal expression of long non-coding RNAs (lncRNAs) affects the tumor immune microenvironment and behavior of breast cancer. This review highlights the role of lncRNAs as modulators of the antitumoral immune response and immune microenvironment in breast cancer, and suggests their potential as biomarkers and targets for immunotherapy.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Felix J. Hartmann, Dunja Mrdjen, Erin McCaffrey, David R. Glass, Noah F. Greenwald, Anusha Bharadwaj, Zumana Khair, Sanne G. S. Verberk, Alex Baranski, Reema Baskar, William Graf, David Van Valen, Jan Van den Bossche, Michael Angelo, Sean C. Bendall
Summary: This antibody-based method allows profiling of metabolic protein expression and regulation in single cells using mass spectrometry while characterizing cellular phenotype. The approach was validated using mass cytometry and applied to clinical samples, revealing tissue-restricted, metabolically repressed immune cells in human colorectal carcinoma. The combination with multiplexed ion beam imaging unveiled the spatial organization of metabolic programs in human tissues.
NATURE BIOTECHNOLOGY
(2021)
Article
Biochemical Research Methods
Zijian Feng, Yuli Hu, Xin Wang, Yiyang Li, Youyi Yu, Jie He, Hongxia Li, Ting Zhang, Lulu Zhang, Guangxia Shen, Xianting Ding
Summary: In this study, a rapid semi-quantitative method for in situ imaging of bacteria and multiplex cell phenotypes on the same solid tumor tissue sections was developed. The findings reveal that breast cancer cells are commonly enriched with Gram-negative bacteria and almost absent of Gram-positive bacteria, regardless of the cancer subtypes. The presence of Gram-negative bacteria has a significant correlation with CD8+ T-cells in HER2+ breast cancer.
Article
Immunology
Xuezheng Xu, Zhizhong Liu, Yi Li, Liqing Fan, Shuang Wang, Jie Guo, Yanwei Luo, Hao Bo
Summary: This study comprehensively delineates the intra-tumoral heterogeneity and tumor microenvironment of testicular embryonic rhabdomyosarcoma (ERMS) using single-nuclear RNA sequencing. Different subtypes with varying proliferative and migratory potentials were identified. Differentially expressed genes were found to be closely related to cell adhesion and extracellular matrix signaling pathways. Additionally, the collagen-related gene COL6A1 played a crucial role in the initiation and progression of ERMS.
JOURNAL OF INFLAMMATION RESEARCH
(2022)
