Article
Biochemistry & Molecular Biology
Sebastian Reiprich, Elif Akova, Attila Aszodi, Veronika Schonitzer
Summary: During biomineralization, cells can sense external physical stimuli and adjust their protein composition accordingly. Hyaluronan synthases are proposed to be crucial for cell communication in biomaterials. Fluid shear stress can enhance the expression and activity of HA in bone mineralization pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Meik Neufurth, Shunfeng Wang, Heinz C. Schroeder, Bilal Al-Nawas, Xiaohong Wang, Werner E. G. Mueller
Summary: Three-dimensional printing processes are recognized as important fabrication techniques in tissue engineering. This study proposes a bio-ink formulation enriched with polyphosphate (polyP) to increase the viability and migration propensity of mesenchymal stem cells (MSC). The bio-ink also stimulates the growth and differentiation of MSC to mineral depositing osteoblasts.
Article
Biochemistry & Molecular Biology
Andrea Sorrentino, Emil Malucelli, Francesca Rossi, Concettina Cappadone, Giovanna Farruggia, Claudia Moscheni, Ana J. Perez-Berna, Jose Javier Conesa, Chiara Colletti, Norberto Roveri, Eva Pereiro, Stefano Iotti
Summary: Biomineralization is the process by which living organisms generate organized mineral crystals, especially in human cells the mechanism and propagation of the mineral remain largely unexplained and controversial. This study provides insight into the calcium depositions in human bone mesenchymal stem cells, suggesting a potentially redefined model for biomineralization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Naoki Ishiuchi, Ayumu Nakashima, Satoshi Maeda, Yoshie Miura, Kisho Miyasako, Kensuke Sasaki, Toshio Uchiki, Ayano Sasaki, Shogo Nagamatsu, Naoki Nakao, Masataka Nagao, Takao Masaki
Summary: Fibrosis is a common histological feature in chronic organ injury, leading to organ failure. Adipose-derived mesenchymal stem cells (ASCs) have been studied as a therapeutic tool to prevent organ failure. This study compared the therapeutic effects of ASCs from superficial and deep layers and found that both types of ASCs are equally effective and safe for treating organ injury.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Biochemistry & Molecular Biology
Alexandra Calle, Maria Zamora-Ceballos, Juan Barcena, Esther Blanco, Miguel angel Ramirez
Summary: This study reports the isolation and characterization of new rabbit mesenchymal stem cell (rMSC) lines from different tissue sources. The rMSC lines showed similar morphology, expression of cell surface and intracellular markers, as well as differentiation capacity under different conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Chiung-Chyi Shen, Shan-hui Hsu, Kai-Bo Chang, Chun-An Yeh, Hsiang-Chun Chang, Cheng-Ming Tang, Yi-Chin Yang, Hsien-Hsu Hsieh, Huey-Shan Hung
Summary: This study demonstrates the superior biological properties and biocompatibility of PEG-HA-AuNP nanocomposites for cell behavior in both MC3T3-E1 cells and MSCs, as well as their potential in promoting anti-inflammatory response and endothelialization when implanted into rats subcutaneously for a period of 1 month.
Article
Chemistry, Multidisciplinary
Hiroki Miyajima, Hiroki Touji, Kazutoshi Iijima
Summary: Bone-like hydroxyapatite (HAp) was prepared from 1.5SBF with different pH values, resulting in micro-sized particles with higher Ca/P ratio and crystallinity at pH 7.4, and nano-sized particles at pH 8.0 and 8.4. A mixture of micro-sized and nano-sized particles was obtained at pH 7.7. Monitoring Ca2+ concentrations showed a drastic decrease at pH 7.4, slightly decreased at higher pH, and higher at pH 7.7. The cytotoxicity of the obtained precipitates to mesenchymal stem cells was lower than that of synthetic HAp.
Article
Dentistry, Oral Surgery & Medicine
Cristiane Duque, Hebatullah Hussein, Janaina Bortolatto, Anuradha Prakki, Anil Kishen
Summary: This study evaluated the effects of taxifolin and EGCG on stem cells from the apical papilla. The results showed that at lower concentrations, taxifolin and EGCG were compatible with the cells and increased the expression of mineralization markers.
ARCHIVES OF ORAL BIOLOGY
(2022)
Article
Engineering, Biomedical
Ebrahim Rahmani-Moghadam, Tahereh Talaei-Khozani, Vahideh Zarrin, Zahra Vojdani
Summary: The study found that the combination of TQ and hydroxyapatite has a synergistic effect on the differentiation of mesenchymal stem cells. Alginate can slow down scaffold degradation rate and enhance mechanical strength.
BIOMEDICAL ENGINEERING ONLINE
(2021)
Article
Dentistry, Oral Surgery & Medicine
Alaa M. Mansour, Sarah Yahia, Hassan Reda Hassan Elsayed, Saied A. E. El-Attar, Mohammed E. Grawish, Youssry M. El-Hawary, Ibrahim M. El-Sherbiny
Summary: The newly developed PCL-based nanofibrous composite scaffold showed enhanced periodontal wound healing/regeneration of class II furcation defects. Moreover, the combination of ADSCs seeded onto the scaffold further enhanced the periodontal tissues regeneration, demonstrating significant improvements in defect dimensions and tissue quality compared to the control group.
CLINICAL ORAL INVESTIGATIONS
(2022)
Article
Materials Science, Paper & Wood
Mehdi Sadat-Shojai, Milad Asadnia, Nehleh Zarei-fard, Amir-Reza Arvaneh
Summary: This study aimed to fabricate defect-free fiber mats using electrospinning technique, with naturally occurring cellulose from banana pseudo-stem as a starting material. Hydroxyapatite nanoparticles were incorporated into the cellulose to enhance its mechanical properties and bioactivity, resulting in a promising scaffold for bone regeneration applications.
Article
Cell Biology
Aijia Cai, Zeng-Ming Zheng, Marcus Himmler, Dirk W. Schubert, Thomas A. Fuchsluger, Volker Weisbach, Raymund E. Horch, Andreas Arkudas
Summary: This study investigates the use of co-cultured Schwann cells (SCs) with myoblasts/adipogenic mesenchymal stromal cells (Mb/ADSC) for skeletal muscle tissue engineering. The results suggest that SCs promote the myogenic differentiation of Mb/ADSC, and the co-culture on electrospun nanofibers shows promising potential for future clinical applications.
Article
Cell Biology
Jeevithan Elango, Rodion Bushin, Artiom Lijnev, Piedad N. De Aza, Carlos Perez-Albacete Martinez, Jose Manuel Granero Marin, Ana Belen Hernandez, Luis Ramon Meseguer Olmo, Jose Eduardo Mate Sanchez De Val
Summary: Hydroxyapatite (HA) is a mineral component used for repair and regeneration of mineralized tissues. The addition of germanium (Ge) improves the properties of HA and shows biocompatibility with BM-MSCs.
Article
Biotechnology & Applied Microbiology
Sonali Rawat, Krishan Gopal Jain, Deepika Gupta, Pawan Kumar Raghav, Rituparna Chaudhuri, Pinky, Adeeba Shakeel, Varun Arora, Harshita Sharma, Debika Debnath, Ankarao Kalluri, Ashwini K. Agrawal, Manjeet Jassal, Amit K. Dinda, Prabir Patra, Sujata Mohanty
Summary: The study successfully differentiated mesenchymal stem cells into dopaminergic neurons using PCL-G nanocomposites, showing promising potential. The newly differentiated neurons exhibited unique features, including enhanced neuronal arborization, intracellular Ca2+ influx, and dopamine secretion.
Review
Biochemistry & Molecular Biology
Yessica Zamudio-Cuevas, Ricardo Plata-Rodriguez, Javier Fernandez-Torres, Karina Martinez Flores, Victor Hugo Cardenas-Soria, Anell Olivos-Meza, Adriana Hernandez-Rangel, Carlos Landa-Solis
Summary: This review examines the general aspects of the synovial membrane and the specific aspects of synovial membrane mesenchymal stem cells (S-MSCs), with a focus on their composition and location. The review also discusses the current clinical applications of S-MSCs in joint tissue repair, including their regenerative properties and immunomodulatory mechanisms. Despite limited interest in new clinical trials, there is evidence supporting the use of S-MSCs for cartilage repair.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Chemistry, Multidisciplinary
Xiangyu Liu, Mei Yang, Fang Lei, Yaru Wang, Mingying Yang, Chuanbin Mao
Summary: Filamentous phages engineered to display RGD peptide on silk protein microparticles can promote adhesion, proliferation, and differentiation of neural stem cells, stimulate angiogenesis and neurogenesis, and lead to brain-tissue regeneration and functional recovery in stroke therapy.
ADVANCED MATERIALS
(2022)
Article
Nanoscience & Nanotechnology
Ruyin Lyu, Yuping Chen, Yajun Shuai, Jie Wang, Leihao Lu, Qichao Cheng, Jiangfeng Cai, Chuanbin Mao, Mingying Yang
Summary: We demonstrate a universal strategy for discovering peptides that can bind to and functionalize biomaterials using a Bombyx mori silk fibroin membrane as a model. By fusing two peptides, one screened for binding to the biomaterial and another derived from a growth factor, a new chimeric peptide is created to efficiently bind to the silk fibroin membrane. The chimeric peptide enables the membrane to induce osteogenic differentiation of human mesenchymal stem cells and stimulate bone regeneration in a rat model without causing inflammation.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Engineering, Biomedical
Hao Zhang, Ren Wang, Chao Wu, Wei Feng, Qixin Zhong, Xianfu Chen, Tao Wang, Chuanbin Mao
Summary: This study reports the self-assembly of milk casein and rice protein into protein nanoparticles with controllable release profiles. These nanoparticles can be efficiently uptaken by breast cancer cells and effectively kill cancer cells. The solid core of the nanoparticles completely inhibits tumor growth. This research opens up a new avenue for producing biomaterials with controllable interior topologies relevant to drug release profiles through diffusion-mediated nanoscale carving.
Article
Materials Science, Multidisciplinary
Qing Bao, Ying Zhang, Xiangyu Liu, Tao Yang, Hui Yue, Mingying Yang, Chuanbin Mao
Summary: Due to the complexity and heterogeneity of cancer, there is a high demand for clear and accurate tumor imaging and image-guided multimodal synergistic therapy. In this work, bismuth-based mesoporous silica-coated nanoparticles (BMSNs) were successfully fabricated with a thin silica shell and modified with an MCF-7 breast cancer-targeting peptide. The resulting nanoparticles showed enhanced drug loading and aggregation in the tumor site, enabling enhanced computed tomography imaging and synergistic chemo-photothermal therapy.
ADVANCED OPTICAL MATERIALS
(2023)
Article
Materials Science, Biomaterials
Yingfan Chen, Xiangyu Liu, Mingying Yang, Weilian Sun, Chuanbin Mao
Summary: Peripheral nerve injury (PNI) leads to persistent abnormalities in sensory function. The current treatment methods for large nerve defects are not effective. In this study, a genetically engineered virus nanofiber was developed as an artificial nerve fiber graft, which promoted nerve regeneration and neurogenesis in a rat model. This phage-based biomaterial shows potential as a substitute for autologous nerve grafts (ANGs) in treating PNI.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Engineering, Biomedical
Chen Zhuang, Guanglin Zhu, Yingjun Wang, Lin Wang, Xuetao Shi, Chuanbin Mao
Summary: Insoluble metal bisphosphonates (BPs) are developed through crystallization on a solid calcium hydroxyapatite (HA)-based substrate, presenting high purity, regular morphologies and excellent biodegradability. These CaBPs can induce osteogenic differentiation of adipose-derived mesenchymal stem cells in vitro and stimulate bone formation effectively in a femur defect rabbit model, showing great potential as biomaterials for tissue regeneration.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Nanoscience & Nanotechnology
Chuanbin Mao, Songyuan Yao, Lin Wang, Penghe Qiu
Summary: Considerable progress has been made on the synthetic chemistry and antibacterial application of tricobalt tetroxide (Co3O4) nanomaterials. However, the current approaches to synthesizing Co3O4 nanomaterials are complex. In this study, a one-pot strategy was developed to synthesize Co3O4 nanowires with antibacterial activity using engineered bacterial flagella as a biotemplate. The flagella displaying negatively charged peptides effectively induced the nucleation of Co3O4 nanoparticles on their surface to form polycrystalline nanowires. The E20-flagella showed higher effectiveness than the E10-flagella.
ACS APPLIED NANO MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Leihao Lu, Qing Bao, Yao Miao, Qichao Cheng, Yuyin Chen, Shuxu Yang, Chuanbin Mao, Mingying Yang
Summary: Thrombotic disease is a serious health threat that requires targeted therapy and precise thrombus localization. A nanoparticle called Mp-Bi@SiO2-GK has been developed to address the limitations of traditional drugs. It combines thrombus targeting, computed tomography (CT) imaging, photothermal therapy, and chemotherapy to enhance thrombolysis efficiency without harming tissues or affecting organ metabolism.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Biophysics
Jinxiu Hou, Xuejia Qian, Yi Xu, Zhirui Guo, Benjamin Thierry, Chih-Tsung Yang, Xin Zhou, Chuanbin Mao
Summary: A rapid and ultrasensitive optical biosensing scheme was developed to detect avian influenza virus H9N2. The scheme used a genetically engineered filamentous M13 phage probe to enhance the detection signal. The phage-based strategy could detect H9N2 viruses in real samples within 10 minutes, even at low concentrations.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Yueyi Sun, Xuewei Qu, Penghe Qiu, Chuanbin Mao
Summary: Molecular beacons (MBs) are DNA-based probes used for detecting DNA or RNA fragments. A nanoparticle-based MB (NPMB) utilizing upconversion nanoparticles (UCNPs) as fluorophores is proposed to avoid background autofluorescence and enable the detection of small RNA from complex clinical samples. The NPMB demonstrates ultrasensitive detection of small nucleic acid biomarkers in a range of clinical samples.
Article
Chemistry, Multidisciplinary
Yuping Chen, Ruyin Lyu, Jie Wang, Qichao Cheng, Yanfang Yu, Shuxu Yang, Chuanbin Mao, Mingying Yang
Summary: This study presents a novel nanoplatform constructed using zeolitic imidazolate framework-8 (ZIF-8) mediated by silk fibroin (SF), modified with a newly discovered MCF-7 breast tumor-targeting peptide (AR peptide). The nanoplatform loaded with indocyanine green (ICG) and doxorubicin (DOX) enables targeted and controlled drug delivery for multimodal therapy, including photodynamic therapy (PDT) and photothermal therapy (PTT), as well as chemotherapy. The results demonstrate the promising potential of the nanoplatform for targeted and effective treatment of breast tumors with minimal side effects.
Article
Chemistry, Multidisciplinary
Na Li, Ying Zhang, Tiancheng Wei, Tao Yang, Qing Bao, Qichao Cheng, Chuanbin Mao, Yajun Shuai, Mingying Yang
Summary: We designed a fluorescence-based aptamer biosensor for the fast, simple, low-cost, and sensitive detection of Staphylococcus aureus. The aptasensor performed well in real food samples (milk) and chicken muscles for S. aureus detection.
Article
Nanoscience & Nanotechnology
Jie Guo, Zihao Wu, Yin Jia, Hongwei Cao, Qin Qin, Tingting Sun, Xinru Zhou, Xue Pan, Cheng Hua, Chuanbin Mao, Shanrong Liu
Summary: A Bayesian neural network model (BNN-CRC15) trained on routine laboratory data was developed for colorectal cancer prediction. BNN-CRC15 provided quantified prediction uncertainty, allowing identification of a confident subset of participants, for which BNN-CRC15 exhibited superior performance, mimicking the diagnostic process of physicians. These findings suggest that BNN-CRC15 could be a valuable tool for improving colorectal cancer diagnosis and prevention.
Article
Chemistry, Multidisciplinary
Lihua Li, Lingling Chen, Yao Lu, Binglin Li, Rong Hu, Ling Huang, Tao Zhang, Xiaoming Wei, Zhongmin Yang, Chuanbin Mao
Summary: This study reports a method of using macrophages loaded with aggregated carbon dots to treat sepsis. These carbon dots have enhanced antibacterial ability and anti-inflammatory effects, making them effective in treating infections caused by multidrug-resistant bacteria.
Article
Chemistry, Multidisciplinary
Xiangyu Liu, Puxian Xiong, Lejing Li, Mei Yang, Mingying Yang, Chuanbin Mao
Summary: In this study, a near-infrared mechanoluminescent material was used to modify artificial vascular grafts, allowing for real-time monitoring of cardiovascular disease models. The material exhibited good blood and tissue compatibility and the near-infrared signals emitted were linearly correlated with the severity of the diseases.
MATERIALS HORIZONS
(2022)
