Article
Biochemistry & Molecular Biology
Zhiming Yang, Tianxiang Xiao, Kai Lu
Summary: In this study, it was found that inhibiting the activity of UGT enzyme effectively increased the toxicity of chlorpyrifos to the chlorpyrifos-resistant strain of Nilaparvata lugens. The resistant strain showed higher content of UGT compared to the susceptible strain. Four UGT genes (UGT386H2, UGT386J2, UGT386N2, and UGT386P1) were significantly overexpressed in the resistant strain and could be induced by chlorpyrifos. Knockdown of UGT386H2 or UGT386P1 increased the susceptibility of the resistant strain to chlorpyrifos. These findings suggest that overexpression of these two UGT genes contributes to chlorpyrifos resistance in N. lugens.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Entomology
Kaikai Mao, Zhijie Ren, Wenhao Li, Tingwei Cai, Xueying Qin, Hu Wan, Byung Rae Jin, Shun He, Jianhong Li
Summary: Carboxylesterases (CarEs) are an important detoxification enzyme family in insecticide resistance, with specific genes in the insect Nilaparvata lugens being identified and shown to be induced by various insecticides. Tissue-specific expression analysis showed certain CarE genes are highly expressed in the midgut and fat body, while others are more highly expressed in the head. The NlCarE1 gene was identified as a main candidate gene for nitenpyram resistance, with evidence suggesting a cooperative relationship with NlCarE19 in resistance formation. These findings provide a foundation for further understanding of insecticide resistance mediated by CarE in N. lugens.
Article
Agronomy
Xin-Yu Song, Yu-Xuan Peng, Li-Xiang Wang, Wen-Nan Ye, Xin-Guo Pei, Yan-Chao Zhang, Shuai Zhang, Cong-Fen Gao, Shun-Fan Wu
Summary: Nilaparvata lugens has developed a high level of resistance to pymetrozine. Pymetrozine-resistant brown planthopper showed cross-resistance with some neonicotinoids such as dinotefuran, nitenpyram, and sulfoxaflor. The resistance to pymetrozine in N. lugens was found to be polygenic, autosomal, and incompletely dominant. The fitness costs associated with pymetrozine resistance can be utilized in insect resistance management strategies to prolong the effectiveness of pymetrozine for controlling N. lugens in China.
PEST MANAGEMENT SCIENCE
(2022)
Article
Environmental Sciences
Kai Lu, Yimin Li, Tianxiang Xiao, Zhongxiang Sun
Summary: This study analyzed the expression patterns of CarE genes in chlorpyrifos-resistant strains, showing that CarE3, CarE17, and CarE19 were overexpressed. Knockdown of CarE17 or its upstream transcription factors Lim1β and C15 reduced the resistance of N. lugens to chlorpyrifos.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Agronomy
Juel Datta, Qi Wei, Qixin Yang, Pin-Jun Wan, Jia-Chun He, Wei-Xia Wang, Feng-Xiang Lai, Md Panna Ali, Qiang Fu
Summary: The study revealed high levels of resistance in Chinese populations of Nilaparvata lugens to imidacloprid, thiamethoxam, while the first report of insecticide resistance in Bangladesh showed similarly high levels of resistance to these insecticides. Significant differences in resistance levels to certain insecticides between the two countries were observed in 2020.
Article
Agriculture, Multidisciplinary
Yixi Zhang, Baojun Yang, Zhiming Yang, Lu Kai, Zewen Liu
Summary: Two P450 enzymes, CYP4C62 and CYP6BD12, in Nilaparvata lugens were found to bioactivate chlorpyrifos into its active ingredient chlorpyrifos-oxon in vivo and in vitro. Knockdown of these two genes reduced sensitivity to chlorpyrifos and the formation of chlorpyrifos-oxon in N. lugens. This study revealed a novel mechanism of insecticide resistance due to the reduction in bioactivation.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agronomy
Li-Xiang Wang, Sha Tao, Yan-Chao Zhang, Xin-Guo Pei, Yang Gao, Xin-Yu Song, Zhi-Tao Yu, Cong-Fen Gao
Summary: Upregulation of NlMdr49-like gene contributes to imidacloprid resistance in field populations of brown planthopper, as indicated by transcriptome analysis. Functional enrichment analysis revealed that the differentially expressed genes are mainly linked to metabolic process and transmembrane transport, highlighting the importance of NlMdr49-like in resistance development.
PEST MANAGEMENT SCIENCE
(2022)
Article
Agronomy
Yan-Chao Zhang, Yang Gao, Wen-Nan Ye, Yu-Xuan Peng, Kun Yan Zhu, Cong-Fen Gao
Summary: In this study, the CRISPR/Cas9 system was used to disrupt the P450 gene NlCYP6CS1 and its role in insecticide resistance in brown planthopper was elucidated. The knockout strain Nl6CS1-KO showed increased susceptibility to certain insecticides compared to the background resistant strain Nl-R. Life table analysis revealed differences in reproductive capacity, nymph survival rate, and body weight between Nl6CS1-KO and Nl-R. This study provides insights into the mechanism of insecticide resistance in brown planthopper and demonstrates the application of CRISPR/Cas9 system in studying similar insects.
PEST MANAGEMENT SCIENCE
(2023)
Review
Biotechnology & Applied Microbiology
Bin Tang, Kangkang Xu, Yongkang Liu, Zhongshi Zhou, Sengodan Karthi, Hong Yang, Can Li
Summary: Although insecticides are widely used in agriculture for pest control, they have resulted in various side effects and challenges such as resistance, resurgence, and residue. Understanding the physiological metabolic resistance of pests like the brown planthopper to insecticides is crucial for developing more effective and harmless alternatives. Mutations in metabolic enzymes and target sites play a significant role in the insensitivity of pests to insecticides.
Article
Agronomy
Kakeru Yokoi, Yuki Nakamura, Akiya Jouraku, Gaku Akiduki, Miwa Uchibori-Asano, Seigo Kuwazaki, Yoshitaka Suetsugu, Takaaki Daimon, Kimiko Yamamoto, Hiroaki Noda, Sachiyo Sanada-Morimura, Masaya Matsumura, Le Quoc Cuong, Ho Van Chien, Gerardo F. Estoy, Tetsuro Shinoda
Summary: The study found that the overexpression of the CYP6ER1 gene played a key role in imidacloprid resistance in the brown planthopper. Two simple and convenient PCR-based molecular diagnostic methods were established to detect the CYP6ER1 gene with a three-nucleotide deletion, showing a relationship between imidacloprid resistance and the CYP6ER1 gene locus.
PEST MANAGEMENT SCIENCE
(2021)
Article
Entomology
Xinyan Liang, Lin Chen, Xiaoying Lan, Guangrong Liao, Ling Feng, Jitong Li, Wenyan Fan, Shuang Wang, Jinglan Liu
Summary: Drought stress impacts the physiology and population growth of brown planthoppers (BPH). BPH feeding on drought-stressed rice have lower population numbers and exhibit negative physiological changes, including decreased water content and osmotic pressure, as well as altered glucose content and glutathione S-transferase (GST) activity. The expression of reproductive-related genes is also affected. Flight muscle size and mitochondrial abundance in BPH are decreased under drought stress.
Article
Biochemistry & Molecular Biology
Zhijie Ren, Tingwei Cai, Yue Wan, Qinghong Zeng, Chengyue Li, Junjie Zhang, Kangsheng Ma, Shun He, Jianhong Li, Hu Wan
Summary: In this study, the structure and function of commensal microbiome in Nilaparvata lugens were observed after exposure to target insecticides, non-target insecticides, and fungicides. The results showed that both non-target insecticides and fungicides can disrupt the structure of the microbiome, affecting the symbiotic bacteria and fungi in different ways.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yiguang Lin, Huijun Ji, Xiaocong Cao, Yongjie Cen, Yumei Chen, Shuangshun Ji, Sichun Zheng
Summary: Insecticides are commonly used to control the serious pest Nilaparvata lugens in rice fields, but their repeated application can have negative effects. A study found that AMP-activated protein kinase (AMPK) plays a crucial role in maintaining energy metabolism and responding to insecticide treatment in N. lugens. Knockdown of NlAMPK alpha significantly enhanced the insecticidal efficiency of pymetrozine against N. lugens.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yixi Zhang, Baojun Yang, Na Yu, Guanghua Luo, Haoli Gao, Xumin Lin, Zewen Liu
Summary: NlGSTs1 and NlGSTs2 in rice brown planthopper play important roles in resistance and defense by enhancing antioxidation and reducing H2O2 levels. The antioxidation activity of these GSTs is higher than their direct detoxification, supporting their ability to remedy oxidative stress. The development of insecticide resistance may enhance the insects' ability to counter oxidative stress, thus reducing the resistance level of rice against the brown planthopper.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Entomology
Xin-Yu Song, Yu-Xuan Peng, Yang Gao, Yan-Chao Zhang, Wen-Nan Ye, Pin-Xuan Lin, Cong-Fen Gao, Shun-Fan Wu
Summary: By utilizing two different methods, this study evaluated the effects of pymetrozine on the fecundity of N. lugens and determined the resistance levels of different populations. The results showed that pymetrozine significantly inhibited the reproductive behavior of N. lugens, but only a few populations exhibited moderate or low levels of resistance, indicating that pymetrozine can still achieve effective control on the next generation of N. lugens.
