Review
Biotechnology & Applied Microbiology
Zeqing Zhao, Jin Liu, Michael D. Weir, Abraham Schneider, Tao Ma, Thomas W. Oates, Hockin H. K. Xu, Ke Zhang, Yuxing Bai
Summary: This review article presents the recent advances in PDLSC-based bone regeneration and periodontal tissue regeneration. By combining PDLSCs with various materials and scaffolds, as well as applying different osteoinductive agents and strategies, PDLSCs demonstrate the potential for bone and periodontal tissue regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Cell Biology
Josefa Alarcon-Apablaza, Ruth Prieto, Mariana Rojas, Ramon Fuentes
Summary: Bone loss often occurs due to trauma, surgery, or congenital malformations. This review examines the potential of mesenchymal stromal cells (MSCs) from the oral cavity for bone regeneration. Different sources of MSCs, such as dental pulp, inflamed dental pulp, exfoliated deciduous teeth, periodontal ligament, periosteal cells, buccal fat pad, and bone-derived MSCs, have been used successfully for repairing bone defects. Additional scaffolds are needed for effective stem cell delivery.
Review
Biochemistry & Molecular Biology
Dusan Holly, Martin Klein, Merita Mazreku, Radoslav Zamborsky, Stefan Polak, L'ubos Danisovic, Maria Csobonyeiova
Summary: Oral and craniofacial bone defects are common, and traditional autologous bone grafting has limitations, making bone tissue engineering combined with stem cell therapy a potential alternative. The number of studies investigating cell-based engineering methods for bone damage reconstruction is increasing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Xinxin Zhang, Xiaorui Jiang, Shan Jiang, Xiyu Cai, Shengji Yu, Guoxian Pei
Summary: This study demonstrated that sensory nerve fibers can promote osteogenesis of tissue-engineered bone grafts (TEBGs) by influencing vascularization and provided cytological evidence for the neurotization of TEBGs.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biotechnology & Applied Microbiology
Siddharth Shanbhag, Ahmad Rashad, Ellen Helgeland Nymark, Salwa Suliman, Catharina de Lange Davies, Andreas Stavropoulos, Anne Isine Bolstad, Kamal Mustafa
Summary: Cell coculture strategies using HUVEC and GPC spheroids in HPLG showed enhanced in vitro sprouting, suggesting a promising approach for promoting angiogenesis in tissue engineering constructs.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
Guang Yang, Bhushan Mahadik, Ji Young Choi, Justine R. Yu, Trevor Mollot, Bin Jiang, Xiaoming He, John P. Fisher
Summary: The study utilized a microfluidic approach to prepare collagen microbeads loaded with human bone marrow derived mesenchymal stem cells for creating engineered bone grafts. The cell-laden microbeads self-assembled into macro-tissues, enabling precise control over cell spatial distribution at the macroscale, offering flexibility for graft volume and architecture. The approach has the potential to create 3D engineered bone grafts with customized vasculature using modular tissue engineering and 3D printing.
ACTA BIOMATERIALIA
(2021)
Article
Cell Biology
Sandra Stamnitz, Agnieszka Krawczenko, Urszula Szalaj, Zaneta Gorecka, Agnieszka Antonczyk, Zdzislaw Kielbowicz, Wojciech Swieszkowski, Witold Lojkowski, Aleksandra Klimczak
Summary: FGF-2 and BMP-2 enhance the osteogenic differentiation potential of MSCs grown on a scaffold, making them promising tools for bone regeneration. This was confirmed through in vitro and in vivo experiments.
Review
Cell Biology
Bo Li, Takehito Ouchi, Yubin Cao, Zhihe Zhao, Yi Men
Summary: MSCs can be found in mammalian teeth from various dental sources, with similar multipotent potential and immunomodulatory functions, showing promising applications in injury repair and disease treatment.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Qiao Ruan, Shenglong Tan, Li Guo, Dandan Ma, Jun Wen
Summary: Prevascularization techniques have been proposed to solve the challenge of rapid vascularization in pulp regeneration. These techniques involve coculturing endothelial cells and pericytes to induce intercellular communication, and then introducing them into a customized artificial vascular bed or inducing self-assembly. However, there are still unresolved problems regarding cell sources, intercellular communication, and the construction of prevascularization systems for pulp regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Engineering, Biomedical
Peng Guo, Xizhe Liu, Penghui Zhang, Zhongyuan He, Zhen Li, Mauro Alini, R. Geoff Richards, Sibylle Grad, Martin J. Stoddart, Guangqian Zhou, Xuenong Zou, Danny Chan, Wei Tian, Dafu Chen, Manman Gao, Zhiyu Zhou, Shaoyu Liu
Summary: This study maps the transcriptional landscapes of different osteogenic microenvironments for mesenchymal stromal cells (MSCs) at single-cell resolution, providing insights into the regulation of bone regeneration by three-dimensional hydroxyapatite (HA) scaffolds and osteogenic medium (OM). The research reveals that HA scaffolds activate osteogenic-related signaling pathways and inhibit adipogenesis and fibrosis, while OM mediates osteogenesis through the ZBTB16 and WNT signaling pathways. The study also demonstrates the heterogeneity of MSCs and provides new directions for improving osteogenic biomaterials.
BIOACTIVE MATERIALS
(2022)
Review
Nanoscience & Nanotechnology
Qi You, Minxun Lu, Zhuangzhuang Li, Yong Zhou, Chongqi Tu
Summary: Congenital or acquired bone defects present a challenge to the global healthcare system, and novel cell sheet technology shows promise for promoting bone regeneration. This review introduces and explores the application of cell sheet technology in bone regeneration, highlights the current state of research, and offers perspectives on translating this knowledge to clinical practice.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2022)
Review
Biotechnology & Applied Microbiology
Rongkai Cao, Beibei Chen, Qianru Li, Piaopiao Qiu, Xiaojie Liang, Yujie Cao
Summary: This study summarizes the characteristics of periosteal cells (PCs) for bone and cartilage regeneration and compares their efficacy with bone marrow stromal cells (BMSCs). The results demonstrate the beneficial effects of PCs in bone regeneration and the need for further research on the cartilage formation capacity of BMSCs.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Kengo Abe, Akihiro Yamashita, Miho Morioka, Nanao Horike, Yoshiaki Takei, Saeko Koyamatsu, Keisuke Okita, Shuichi Matsuda, Noriyuki Tsumaki
Summary: Allogeneic iPSC-derived cartilage organoids survive and integrate with surrounding native cartilage without immune reactions in a primate model of chondral defects in the knee joints, being remodeled and functioning as articular cartilage.
NATURE COMMUNICATIONS
(2023)
Article
Cell & Tissue Engineering
Che-Chang Tu, Nai-Chen Cheng, Jiashing Yu, Yi-Xuan Pan, Wei-Chiu Tai, Yin-Chuan Chen, Po-Chun Chang
Summary: This study evaluates the potential of self-assembled adipose-derived stem cell spheroids (ADsp) in microbial transglutaminase cross-linked gelatin hydrogel (mTG) for treating diabetic periodontal wounds and craniofacial defects. The results show that osteogenically pretreated ADsp-mTG can promote the regeneration of diabetic osseous defects. This suggests that ADsp-mTG can facilitate diabetic periodontal wound healing and craniofacial osseous defect regeneration.
STEM CELL RESEARCH & THERAPY
(2023)
Review
Medicine, Research & Experimental
Mengyuan Wang, Ying Li, Hongqiao Wang, Meng Li, Xiaomin Wang, Rongzhen Liu, Daijun Zhang, Wenhua Xu
Summary: Corneal epithelial defects and excessive wound healing can cause severe complications. Stem cells offer a promising solution for regenerating the corneal epithelium and treating severe corneal epithelial injury. Biological scaffolds such as the amniotic membrane, fibrin, and hydrogels provide the necessary signals for stem cell proliferation and differentiation. Researchers have evaluated these scaffolds as potential therapeutic interventions for corneal disorders, identifying both benefits and drawbacks. This study provides a comprehensive overview of the history and use of stem cells in corneal repair, focusing on biological scaffolds used in stem cell transplantation and innovative materials under investigation.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Materials Science, Biomaterials
Masumeh Staji, Negar Sadeghzadeh, Soheila Zamanlui, Mojgan Azarani, Ali Golchin, Masoud Soleimani, Abdolreza Ardeshirylajimi, Arash Khojasteh, Simzar Hosseinzadeh
Summary: This study investigated the synergistic effect of an electrospun scaffold made of polyurethane, gelatin, and human amniotic polymers in the co-culture of mesenchymal stem cells and human keratinocytes. The results showed that this scaffold promoted cell proliferation and epithelial differentiation, and had a significant influence in mice experiments.
INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS
(2023)
Article
Polymer Science
Reza Samanipour, Sina Farzaneh, Javad Ranjbari, Sheida Hashemi, Arash Khojasteh, Simzar Hosseinzadeh
Summary: In this study, an oxygen-releasing PCL/SP-PVA scaffold was developed and used as a platform for osteogenic differentiation of dental pulp stem cells. The scaffold showed no inhibitory effect on cell growth and provided a suitable matrix for osteogenic differentiation. The results support the use of oxygen-releasing scaffolds as a potential strategy to accelerate bone regeneration.
Article
Chemistry, Applied
Payam Baei, Hamed Daemi, Fatemeh Aramesh, Hossein Baharvand, Mohamadreza Baghaban Eslaminejad
Summary: The purpose of cartilage tissue engineering is to create artificial constructs that mimic native tissue in terms of biological functions and mechanical features, with the aim of improving tissue regeneration. Polysaccharides, due to their structural similarity to the cartilage extracellular matrix (ECM), have attracted attention for developing biomimetic materials. The mechanical properties of constructs and the addition of bioactive molecules play crucial roles in cartilage tissue regeneration. This article discusses polysaccharide-based constructs for cartilage regeneration, with a focus on newly developed biomimetic materials, tuning the mechanical properties of constructs, designing carriers loaded with chondroinductive agents, and the development of appropriate bioinks for bioprinting.
CARBOHYDRATE POLYMERS
(2023)
Article
Engineering, Biomedical
Hannaneh Safiaghdam, Hanieh Nokhbatolfoghahaei, Saeed Farzad-Mohajeri, Mohammad Mehdi Dehghan, Hekmat Farajpour, Hossein Aminianfar, Zeinab Bakhtiari, Massoumeh Jabbari Fakhr, Simzar Hosseinzadeh, Arash Khojasteh
Summary: In this study, the researchers evaluated the effect of adding NanoMgO to PCL/β-TCP scaffolds on bone regeneration. The results suggest that the PCL/β-TCP/nanoMgO scaffold is more suitable as a bone substitute in critical-sized calvarial defects compared to PCL/β-TCP scaffolds.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2023)
Article
Biochemistry & Molecular Biology
Mahsa Ghorbaninejad, Maliheh Khademi-Shirvan, Samaneh Hosseini, Anna Meyfour, Maryam Shahhoseini, Mohamadreza Baghaban Eslaminejad
Summary: In this study, the effect of Curcumin on the expression of EZH2 in osteoblast differentiation was investigated. The results demonstrated that Curcumin could enhance osteogenic differentiation by downregulating the expression of EZH2.
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
(2023)
Article
Chemistry, Applied
Maryam Dehghan-Niri, Ebrahim Vasheghani-Farahani, Mohamadreza Baghaban Eslaminejad, Moslem Tavakol, Fatemeh Bagheri
Summary: Nanocomposite hydrogels based on tyramine conjugated gum tragacanth, PVA, and HNTs were prepared and characterized. The incorporation of HNTs into gum tragacanth was confirmed by FTIR, 1H NMR, and TGA results. The mechanical strength of hydrogels increased with HNTs content, and they showed potential for bone tissue engineering based on biocompatibility and osteogenic activity tested with rabbit bone marrow mesenchymal stem cells.
CARBOHYDRATE POLYMERS
(2023)
Article
Cell Biology
Leila Taghiyar, Hamideh Asadi, Mohamadreza Baghaban Eslaminejad
Summary: This study aims to develop a new bioscaffold of decellularized osteochondral tissue (DOT) that is recellularized by bone marrow-derived mesenchymal stem cells (BM-MSCs), as a biphasic allograft, to preserve the interface between the cartilage section and subchondral bone of the joint. The results showed that the cells successfully penetrated the bone and cartilage lacunas in implanted grafts, proliferated, differentiated into osteoblasts and chondrocytes, and started ECM secretion. The integrity of the cartilage-to-bone border was largely preserved, indicating that ECM-sheeted DOT could be used as a useful scaffold for the regeneration of osteochondral defects.
CELL AND TISSUE BANKING
(2023)
Article
Materials Science, Multidisciplinary
Fereshte Ghafari, Saeed Karbasi, Mohamadreza Baghaban Eslaminejad
Summary: Electrospun nanofiber scaffolds with added alumina nanowires showed enhanced cell adhesion, proliferation, and migration. Various tests were conducted to evaluate the structure, chemical bonds, crystallinity, and mechanical properties of the scaffolds. The presence of alumina significantly improved the tensile strength and functional performance of the scaffolds. These findings suggest that the electrospun PHB-K/AL2O3 scaffolds have great potential for bone tissue engineering.
MATERIALS CHEMISTRY AND PHYSICS
(2023)
Article
Materials Science, Biomaterials
Tahmineh Ghahri, Zeinab Salehi, Sareh Aghajanpour, Mohamadreza Baghaban Eslaminejad, Niloofar Kalantari, Mohammad Akrami, Rassoul Dinarvand, Hae Lin Jang, Mehdi Esfandyari-Manesh
Summary: Despite challenges in fabricating a scaffold for large bone defects, a method utilizing coaxial extrusion-based bioprinting was proposed to mimic the structural unit of compact bone, osteon. The scaffold mimicked the spatial pattern of human umbilical vein endothelial cells (HUVECs) and human mesenchymal stem cells (hMSCs) to imitate Haversian canal and lamella, respectively. The 3D implant scaffold-cell construct showed high cell viability, proliferation, and osteoblastic differentiation, indicating its potential as a bone graft for regeneration.
BIOMATERIALS ADVANCES
(2023)
Article
Biotechnology & Applied Microbiology
Saeed Moghadam Deymeh, Sameereh Hashemi-Najafabadi, Mohamadreza Baghaban-Eslaminejad, Fatemeh Bagheri
Summary: This study co-cultivated human bone marrow mesenchymal stem cells and human umbilical vein endothelial cells on treated electrospun scaffolds in a perfusion bioreactor to investigate the effects of different flow patterns on osteogenesis and angiogenesis. The results showed that the multilayer scaffolds performed better in terms of osteogenesis in the vertical flow mode.
BIOTECHNOLOGY LETTERS
(2023)
Article
Cell Biology
Maryam Hosseinzadeh, Amir Kamali, Mohamadreza Baghaban Eslaminejad, Samaneh Hosseini
Summary: This study investigates the chondrogenic potential and therapeutic effect of extracellular vesicles (EVs) derived from MSCs and chondrocytes in the treatment of osteoarthritis (OA). The results show that EVs obtained from co-cultures of chondrocytes and MSCs have improved matrix production and functional differentiation. In a rat OA model, the group with an increased ratio of chondrocyte-derived EVs to MSC-derived EVs showed better recovery from OA compared to other groups.
CELL AND TISSUE RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Fereshte Ghafari, Saeed Karbasi, Mohamadreza Baghaban Eslaminejad, Forough Azam Sayahpour, Niloofar Kalantari
Summary: This study investigated the impact of alumina nanowire on the physical and biological properties of a polyhydroxybutyratekeratin (PHB-K) electrospun scaffold. PHB-K/alumina nanowire nanocomposite scaffolds were made with an optimal concentration of 3 wt% alumina nanowire using the electrospinning method. The nanocomposite scaffold showed improved degradation rate, bioactivity, cell viability, and gene expression compared to other scaffolds, making it a potential construct for osteogenic induction in bone tissue engineering.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Cell & Tissue Engineering
Mahnaz Babaahmadi, Behnoosh Tayebi, Nima Makvand Gholipour, Mehrnaz Tayebi Kamardi, Sahel Heidari, Hossein Baharvand, Mohamadreza Baghaban Eslaminejad, Ensiyeh Hajizadeh-Saffar, Seyedeh-Nafiseh Hassani
Summary: Rheumatoid arthritis is a chronic and systemic autoimmune disease for which there is currently no cure. MSCs have shown promise in modulating the immune system and could be used as a potential tool for treating autoimmune diseases.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Cell Biology
Behnoosh Tayebi, Mohammad Molazem, Mahnaz Babaahmadi, Erfan Ebrahimi, Mostafa Hajinasrollah, Farhad Mostafaei, Nima Makvand Gholipour, Hossein Baharvand, Mohamadreza Baghaban Eslaminejad, Seyedeh-Nafiseh Hassani, Ensiyeh Hajizadeh-Saffar
Summary: This study compared the advantages of ultrasound-guided percutaneous approach and open-surgery method in the tumor necrosis factor-alpha induced disc degeneration model. The results showed that the ultrasound-guided method can better mimic the chronic characteristics of low back pain and is more ethically accepted.
Article
Pharmacology & Pharmacy
Simzar Hosseinzadeh, Forough Shams, Roya Fattahi, Ghader Nuoroozi, Elnaz Rostami, Lida Shahghasempour, Nasim Salehi-Nik, Mahboubeh Bohlouli, Arash Khojasteh, Nazanin Ghasemi, Habibollah Peiravi
Summary: A hemocompatible substrate enables vascular endothelial cells to produce nitric oxide (NO) in response to inflammation after injuries, inhibiting platelet aggregation in small-diameter vessels. By modifying the surface of polyurethane (PU) films, the concentrations of gelatin-aspirin and gelatin-heparin were optimized for enhanced biocompatibility and bioactivity.
ADVANCED PHARMACEUTICAL BULLETIN
(2023)
