Article
Engineering, Biomedical
Zhengbo Wen, Yuxin Chen, Peilin Liao, Fengyu Wang, Weiping Zeng, Shoupei Liu, Haibing Wu, Ning Wang, Lorenzo Moroni, Minmin Zhang, Yuyou Duan, Honglin Chen
Summary: In this study, an in situ cell electrospinning system was developed as an attractive approach for stem cell delivery, which could promote cutaneous wound healing through direct deposition of bioactive fish gelatin fibers and MSCs onto wound sites, leading to a synergistic therapeutic effect.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Nanoscience & Nanotechnology
Jin-Oh Jeong, Young Min Ju, Hyun-Wook Kang, Anthony Atala, James J. Yoo, Sang Jin Lee
Summary: The development of innovative vascular substitutes is increasingly important due to the prevalence of vascular diseases. In this study, a biofunctionalized electrospun vascular scaffold was designed by chemically conjugating heparin molecules and an endothelial cell (EC)-specific antibody. The findings showed that a higher degree of heparin conjugation improved the antithrombotic properties of the vascular scaffold.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Engineering, Biomedical
Isabel Mayoral, Elisa Bevilacqua, Gorka Gomez, Abdelkrim Hmadcha, Ignacio Gonzalez-Loscertales, Esther Reina, Julio Sotelo, Antonia Dominguez, Pedro Perez-Alcantara, Younes Smani, Patricia Gonzalez-Puertas, Ana Mendez, Sergio Uribe, Tarik Smani, Antonio Ordonez, Israel Valverde
Summary: In this study, a patient-specific patch was designed using a hybrid 3D printing technique and VSMC differentiation. Through computational modeling and experimental evaluation, the patch demonstrated favorable hemodynamic characteristics, mechanical properties, cell infiltration, viability, and functionality. This innovative 3D biotechnology holds great potential for applications in regenerative medicine and heart disease prevention.
MATERIALS TODAY BIO
(2022)
Review
Pharmacology & Pharmacy
Yasaman Aghazadeh, Safwat T. Khan, Blessing Nkennor, Sara S. Nunes
Summary: Cell-based vascularization strategies show clinical potential but face challenges and obstacles.
PHARMACOLOGY & THERAPEUTICS
(2022)
Article
Biochemistry & Molecular Biology
Mattia Albiero, Marianna D'Anna, Benedetta Maria Bonora, Gaia Zuccolotto, Antonio Rosato, Marco Giorgio, Elisabetta Iori, Angelo Avogaro, Gian Paolo Fadini
Summary: For the first time, this study explores the hematopoietic and nonhematopoietic roles of p66Shc in regulating HSPC traffic and ischemic responses. Hematopoietic deletion of p66Shc is sufficient to rescue HSPC mobilization and homing in diabetes after ischemia and improves blood flow recovery. Inhibiting p66Shc in blood cells may be a novel strategy to counter PAD in diabetes.
ANTIOXIDANTS & REDOX SIGNALING
(2022)
Article
Materials Science, Multidisciplinary
Jing Wang, Chunxiang Feng, Yunpeng Zhu, Zhixian Wang, Xiang Ren, Xing Li, Yisheng Ying, Yiqun Tian, Kai Yu, Shiliang Liu, Chang Liu, Xiaoyong Zeng
Summary: Bladder tissue engineering using adipose tissue extract (ATE) shows potential for promoting pluripotent marker proteins in stem cells and enhancing proliferation, migration, and angiogenesis in endothelial cells. The integration of electrospun nanofibers and hyaluronic acid colloidal solution in a patch demonstrates feasibility and effectiveness in repairing bladder defects in a rat model, highlighting its potential for tissue engineering and regenerative medicine.
MATERIALS & DESIGN
(2022)
Article
Engineering, Multidisciplinary
Yifan Wu, Lili Song, Muhammad Shafiq, Hiroyuki Ijima, Soo Hyun Kim, Ran Wei, Deling Kong, Xiumei Mo, Kai Wang
Summary: Cardiovascular injuries have significant global morbidity and mortality rates. Synthetic biodegradable polymers offer a promising platform for arterial reconstructions, but current graft options have limitations. This study explored the use of VEGF-binding peptide and SDF-1a peptide to enhance neovascularization and stem/progenitor cell mobilization for vascular regeneration. The peptides were successfully conjugated with a polymer and blended with a high molecular weight polymer to create bioactive vascular grafts. In vivo tests showed that the dual peptide modified grafts had superior patency and tissue regeneration compared to control grafts. These findings have implications for the in situ regeneration of artificial blood vessels.
COMPOSITES PART B-ENGINEERING
(2023)
Article
Hematology
Liming Zhao, Andrew S. Lee, Koki Sasagawa, Jan Sokol, Yuting Wang, Ryan C. Ransom, Xin Zhao, Chao Ma, Holly M. Steininger, Lauren S. Koepke, Mimi R. Borrelli, Rachel E. Brewer, Lorene L. Y. Lee, Xianxi Huang, Thomas H. Ambrosi, Rahul Sinha, Malachia Y. Hoover, Jun Seita, Irving L. Weissman, Joseph C. Wu, Derrick C. Wan, Jun Xiao, Michael T. Longaker, Patricia K. Nguyen, Charles K. F. Chan
Summary: The study identified two populations of vascular stem/progenitor cells (VSPCs) from adipose-derived mesenchymal stromal cells. Cotransplantation of VSPC1 and VSPC2 could form functional vessels and improve perfusion in the mouse hindlimb ischemia model.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2023)
Article
Engineering, Biomedical
Lingling Shang, Ziqi Liu, Baojin Ma, Jinlong Shao, Bing Wang, Chenxi Ma, Shaohua Ge
Summary: The study developed a dual-functional structure, DMOG/nSi-PLGA fibrous membranes, through electrospinning for periodontal tissue regeneration. These membranes exhibited good mechanical properties and biocompatibility, enhanced osteogenesis and angiogenesis, and regenerated the cementum-ligament-bone complex in periodontal defects.
BIOACTIVE MATERIALS
(2021)
Review
Cardiac & Cardiovascular Systems
Bruna B. J. Leal, Naohiro Wakabayashi, Kyohei Oyama, Hiroyuki Kamiya, Daikelly I. Braghirolli, Patricia Pranke
Summary: Cardiovascular disease is the leading cause of death globally, with vascular grafts being a treatment option. Research has shown the urgent need for the development of small-caliber synthetic vascular grafts to overcome the limitations of autologous grafts.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Engineering, Biomedical
Zhengchao Yuan, Dandan Sheng, Liping Jiang, Muhammad Shafiq, Atta ur Rehman Khan, Rashida Hashim, Yujie Chen, Baojie Li, Xianrui Xie, Jun Chen, Yosry Morsi, Xiumei Mo, Shiyi Chen
Summary: This study developed VEGF-binding aligned electrospun PCL/Gel scaffolds by incorporating BP sequence and demonstrated their potential in promoting ligament regeneration. The functionalized scaffolds accumulated VEGF, enhanced tubular formation of HUVECs and wound healing in vitro, and facilitated the regeneration of matured collagen in patellar ligament in vivo.
ACTA BIOMATERIALIA
(2022)
Article
Pharmacology & Pharmacy
Rossella Dorati, Silvia Pisani, Enrica Chiesa, Ida Genta, Giovanna Bruni, Tiziana Modena, Bice Conti
Summary: Polymeric tubular vascular grafts manufactured using electrospinning technique have the potential to serve as an alternative to autologous vascular grafts for treating peripheral artery occlusive disease. By combining different biocompatible and biodegradable polymers in various design approaches, researchers were able to produce prototypes with optimal mechanical behavior and cell interaction for tissue regeneration purposes.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Engineering, Biomedical
Atsushi Mahara, Manabu Shirai, Raghav Soni, Hue Thi Le, Kaito Shimizu, Yoshiaki Hirano, Tetsuji Yamaoka
Summary: This study found that blood-circulating monocytes reconstruct vascular-like tissues through integrin signaling. Using a modified acellular graft, captured monocytes expressed CD163, CD14, and CD16 and exhibited proliferative and migratory abilities. Although this finding provides clear evidence for the early mechanisms of vascular remodeling, the mechanisms of vascular tissue regeneration remain incompletely understood.
MATERIALS TODAY BIO
(2023)
Review
Medicine, Research & Experimental
Hongbing Lin, Huishan Chen, Xuetao Zhao, Zhen Chen, Peipei Zhang, Yue Tian, Yawei Wang, Tong Ding, Lijing Wang, Yuqin Shen
Summary: Periodontitis is a chronic inflammatory disease that poses a challenge for complete regeneration in clinics. Mesenchymal stem cells (MSCs) show potential for regenerating periodontal tissue, but their direct use faces drawbacks such as low survival rate and host immune reactions. MSC culture conditioned medium (CM) offers a promising indirect treatment approach with the potential to overcome these challenges for periodontal tissue regeneration.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Agnieszka Krawczenko, Aleksandra Klimczak
Summary: MSCs, known for their regenerative and immunomodulatory properties, are typically isolated from bone marrow and adipose tissue. This review focuses on MSCs derived from adipose tissue and their secretome in regeneration processes, with a special emphasis on their contribution to angiogenic processes. Various clinical trials have been registered to explore the application of AT-MSCs and their secretome in treating different medical conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Chemistry, Analytical
Yanli Wang, Wenjing Qin, Xiaoyu Hu, Zhongsheng Liu, Zhixin Ren, Huanqi Cao, Baigang An, Xiang Zhou, Muhammad Shafiq, Shougen Yin, Zunfeng Liu
Summary: Researchers have developed a flexible sensor that can monitor human motion and be applied in the field of soft robotics. The sensor exhibits high sensitivity, good linearity, fast response time, and stability. Additionally, they have manufactured a soft robot with integrated sensors for real-time status detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Engineering, Biomedical
Yujie Chen, Wei Xu, Muhammad Shafiq, Daiying Song, Tao Wang, Zhengchao Yuan, Xianrui Xie, Xiao Yu, Yihong Shen, Binbin Sun, Yu Liu, Xiumei Mo
Summary: This study fabricated injectable gelatin/poly(L-lactide)-based nanofibrous microspheres (TMS) and metal phenolic networks (TSMS) to enhance the bioactivity for osteoarthritis (OA) therapy. TSMS exhibited stable porous structure and sustained release of TA, showing good cytocompatibility and anti-inflammatory properties. TSMS also demonstrated significant repair outcome and inhibition of cartilage degradation in vivo.
ACTA BIOMATERIALIA
(2023)
Article
Materials Science, Biomaterials
Mingyue Liu, Xiangsheng Wang, Jie Cui, Hongsheng Wang, Binbin Sun, Jufang Zhang, Bernd Rolauffs, Muhammad Shafiq, Xiumei Mo, Zhanyong Zhu, Jinglei Wu
Summary: In this study, a magnesium-doped silica bioactive glass (SiO2/MgO) nanofiber membrane was prepared by electrospinning. The results showed that the SiO2/MgO nanofiber membrane had good flexibility and hydrophilicity, enabling intimate contact with wound beds. In vitro assessments demonstrated its cytocompatibility, bioactivity, antibacterial activity, and ability to modulate cellular inflammatory response, enhancing cell proliferation and angiogenesis. In a mouse model, it effectively inhibited bacterial infection, down-regulated pro-inflammatory factors, up-regulated anti-inflammatory factors, and enhanced angiogenesis, contributing to the rapid healing of infected wounds.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Review
Chemistry, Multidisciplinary
Jie Cui, Xiao Yu, Yihong Shen, Binbin Sun, Wanxin Guo, Mingyue Liu, Yujie Chen, Li Wang, Xingping Zhou, Muhammad Shafiq, Xiumei Mo
Summary: Tissue engineering has gained widespread attention as a method of producing patient-specific tissue constructs for tissue repair. Different types of scaffold materials have been developed, and the choice of materials should consider their mechanical properties, biodegradability, biocompatibility, and bioresorbability. Inorganic materials, with their broad range of physico-chemical properties, can be used as scaffold fillers and offer a good alternative for tissue engineering. This review focuses on the use of inorganic components in electrospun membranes and emphasizes the biological advantages of incorporating inorganic materials in tissue repair.
Article
Biophysics
Muhammad Shafiq, Zhengchao Yuan, Muhammad Rafique, Shinichi Aishima, Hou Jing, Liang Yuqing, Hiroyuki Ijima, Shichao Jiang, Xiumei Mo
Summary: Skin regeneration is hindered by poor vascularization, inflammation, and scar tissue formation. This study uses multiple bioactive peptides to promote wound healing in both healthy and diabetic rat models. The deployment of these peptides in core/shell fibers effectively induces blood vessel regeneration, resolves inflammation, and recruits host cells for skin repair.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Engineering, Multidisciplinary
Yifan Wu, Lili Song, Muhammad Shafiq, Hiroyuki Ijima, Soo Hyun Kim, Ran Wei, Deling Kong, Xiumei Mo, Kai Wang
Summary: Cardiovascular injuries have significant global morbidity and mortality rates. Synthetic biodegradable polymers offer a promising platform for arterial reconstructions, but current graft options have limitations. This study explored the use of VEGF-binding peptide and SDF-1a peptide to enhance neovascularization and stem/progenitor cell mobilization for vascular regeneration. The peptides were successfully conjugated with a polymer and blended with a high molecular weight polymer to create bioactive vascular grafts. In vivo tests showed that the dual peptide modified grafts had superior patency and tissue regeneration compared to control grafts. These findings have implications for the in situ regeneration of artificial blood vessels.
COMPOSITES PART B-ENGINEERING
(2023)
Article
Materials Science, Multidisciplinary
Mina Shahriari-Khalaji, Muhammad Shafiq, Haitao Cui, Ran Cao, Meifang Zhu
Summary: Cardiovascular diseases (CVDs) are the leading cause of health issues and death globally. Although autografts are the standard treatment, their effectiveness is limited due to various factors. Synthetic vascular grafts have potential for large-diameter blood vessel reconstruction, but lack success for small-diameter substitutes. However, advancements in fabrication technologies have shown promise for small diameter blood vessel manufacturing, offering potential future clinical applications.
APPLIED MATERIALS TODAY
(2023)
Article
Engineering, Biomedical
Yimeng Shu, Ming Ma, Xiaoxia Pan, Muhammad Shafiq, Huizhu Yu, Hangrong Chen
Summary: A multifunctional nano-self-assembly was developed in this study by combining gold nanoparticles, superparamagnetic iron oxide nanoparticles, and cobalt protoporphyrin IX. This nanoassembly can effectively label mesenchymal stem cells and has potential for high-efficient therapy for pulmonary fibrosis.
BIOACTIVE MATERIALS
(2023)
Review
Chemistry, Multidisciplinary
Muhammad Shafiq, Muhammad Rafique, Yingkun Cui, Li Pan, Chi-Wai Do, Emmanuel A. Ho
Summary: Currently, eye diseases are mainly treated with topical eye drops, but there are challenges such as multiple administrations and poor penetration. Therefore, it is necessary to design ocular drug delivery systems that can penetrate the cornea effectively, have good bioavailability, and can target specific regions. Nanocarriers are a promising choice to overcome these barriers and enhance drug penetration into the eye. They can also be modified to improve targeting and stability through functionalization and cell-mediated approaches.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Pharmacology & Pharmacy
Zhengchao Yuan, Lixiang Zhang, Hui Zheng, Muhammad Shafiq, Jiahui Song, Binbin Sun, Mohamed EL-Newehy, Hany EL-Hamshary, Yosry Morsi, Chao Huang, Xiumei Mo, Yuan Xu
Summary: This research explored the beneficial effect of electrospun membranes containing zinc oxide nanoparticles (ZnO NPs) on infectious wound healing. The results showed that these membranes had good cytocompatibility, hemostatic effect, and antibacterial effect. They induced rapid hemostasis, wound regeneration, and reduced wound area in animal models. These findings suggest the potential benefits of electrospun membranes containing ZnO NPs for infectious wound healing.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Article
Materials Science, Biomaterials
Wanxin Guo, Binan Zhao, Muhammad Shafiq, Xiao Yu, Yihong Shen, Jie Cui, Yujie Chen, Pengfei Cai, Zhengchao Yuan, Mohamed EL-Newehy, Hany EL-Hamshary, Yosry Morsi, Binbin Sun, Jianfeng Pan, Xiumei Mo
Summary: The objective of this research was to screen for the effect of different types of bio-elastomers as hemostatic dressings. A PU-TA/Gel sponge was prepared, which showed promising potential in terms of porosity, water absorption ratio, cytocompatibility, hemolysis, clotting time, and wound healing. The sponge rapidly induced stable blood clots and good skin regeneration.
REGENERATIVE BIOMATERIALS
(2023)
Review
Chemistry, Multidisciplinary
Abdul Qadeer Khan, Muhammad Shafiq, Jiatian Li, Kaiqing Yu, Zunfeng Liu, Xiang Zhou, Meifang Zhu
Summary: It is desirable to develop fiber materials with high strength and toughness, but increasing fiber strength often leads to a decrease in toughness. Spider silk, with its excellent combination of high strength and toughness, has inspired the development of artificial fibers mimicking its material and spinning process. This review summarizes the preparation methods of artificial spider silk from different materials, including nonrecombinant proteins, recombinant proteins, polypeptides, synthetic polymers, and polymer nanocomposites. It also discusses various spinning approaches and novel applications of artificial spider silk, such as artificial muscles, sensing, and smart fibers.
Correction
Materials Science, Multidisciplinary
Yi Chen, Zhengchao Yuan, Weiyan Sun, Muhammad Shafiq, Jun Zhu, Jiafei Chen, Hai Tang, Ling Hu, Weikang Lin, Yanxi Zeng, Long Wang, Lei Zhang, Yunlang She, Hui Zheng, Guofang Zhao, Dong Xie, Xiumei Mo, Chang Chen
ADVANCED FIBER MATERIALS
(2023)
Article
Materials Science, Multidisciplinary
Yi Chen, Zhengchao Yuan, Weiyan Sun, Muhammad Shafiq, Jun Zhu, Jiafei Chen, Hai Tang, Ling Hu, Weikang Lin, Yanxi Zeng, Long Wang, Lei Zhang, Yunlang She, Hui Zheng, Guofang Zhao, Dong Xie, Xiumei Mo, Chang Chen
Summary: The researchers developed intelligent multifunctional scaffolds that can recruit VEGF and promote wound healing through angiogenesis and immunomodulation. The scaffolds showed promising results in promoting regeneration in rat wounds, with improved skin appendage neogenesis, sensory function, and collagen remodeling.
ADVANCED FIBER MATERIALS
(2023)
Article
Materials Science, Multidisciplinary
Abdul Qadeer Khan, Kaiqing Yu, Jiatian Li, Xueqi Leng, Meilin Wang, Xuesong Zhang, Baigang An, Bin Fei, Wei Wei, Huichuan Zhuang, Muhammad Shafiq, Lili Bao, Zunfeng Liu, Xiang Zhou
Summary: Smart textiles have the ability to adapt to irregular surfaces and can contract and expand based on the structure of twisted fibers. This technology can be used to create intelligent textiles that can conform to irregular surfaces.
ADVANCED FIBER MATERIALS
(2022)