Article
Food Science & Technology
Yi Yang, Milos Mihajlovic, Manoe J. Janssen, Rosalinde Masereeuw
Summary: This study investigated the role of uremic toxin indoxyl sulfate (IS) in accelerating cellular senescence and promoting kidney fibrosis. The findings suggest that IS accelerates senescence in proximal tubule epithelial cells through TNF-alpha and NF-kappa B signaling early on, and the epithelial-mesenchymal transition process at later time points.
Review
Cell Biology
Ruchi Kumari, Parmjit Jat
Summary: Cellular senescence is a stable cell cycle arrest triggered in response to various stimuli, characterized by dynamic changes in senescent cells. It has both positive effects on tissue repair and anti-cancer mechanisms, but can also negatively impact organismal health. Multiple pathways, including p53/p21 and p16/pRB, play central roles in regulating this process.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Priyanka Banerjee, Niyanshi Gaddam, Tej K. Pandita, Sanjukta Chakraborty
Summary: Cellular senescence, driven by various mechanisms and senescence-associated secretory phenotype (SASP), is an important area of research in age-related diseases. The impact of senescence and SASP on different cell types, the immune system, and the vascular system has been widely discussed, but the impact on lymphatic biology and pathological lymphangiogenesis remains underexplored. In this review, the role of senescence on lymphatic pathobiology, its impact on cancer, and potential therapeutic interventions are discussed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Genetics & Heredity
Kunihiko Futami, Kako Aoyama, Kazuki Fukuda, Masashi Maita, Takayuki Katagiri
Summary: The study showed that DNA demethylation with 5-Aza-dC can induce senescence in telomere-independent fish cell line EPC. Overexpression of HRas gene contributes to early senescence in EPC cells, but may not be sufficient for full senescence. The limited mechanism of Ras-induced senescence may play a role in the senescence-resistance of fish cell lines.
Article
Biochemistry & Molecular Biology
Zimei Yi, Le Ren, Yu Wei, Siyi Chen, Jiechen Zhao, Jiayu Zhu, Junhua Wu
Summary: By building a p21-3MR mouse model, we were able to study the contribution of cells with high p21 expression to tissue dysfunction in aging, obesity, and other pathological conditions, and found that clearing these cells improved multi-organ toxicity. This study is of great importance in further understanding the biology of cell senescence.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Yuki Saito, Maki Miyajima, Sena Yamamoto, Tsukasa Sato, Norihiro Miura, Mineko Fujimiya, Takako S. Chikenji
Summary: Neuropsychiatric manifestations are common in systemic lupus erythematosus (SLE), with depression being observed in a significant percentage of patients. Accumulation of senescent neural cells in the hippocampus contributes to neuropsychiatric SLE, and treatment targeting senescent cells shows promise in alleviating depressive symptoms in SLE mice.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Cell Biology
Runjiu Zhu, Haoyang Wan, Hong Yang, Mingrui Song, Yu Chai, Bin Yu
Summary: With the increasing proportion of older individuals in most countries, the prevalence of osteoporosis has been on the rise, leading scientists to focus on the pathogenic mechanisms of this condition. In recent years, research on cellular senescence has shed light on the role of the senescent microenvironment in osteoporosis. Senescent cells in the bone marrow secrete senescence-associated secretory phenotype (SASP) factors, which can impact both their own function and the surrounding healthy cells, exacerbating the aging process. The composition of SASP may vary depending on the cause of osteoporosis. This review summarizes the relationship between SASP factors and osteoporosis and provides new insights for the investigation of its mechanism.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Cell Biology
Vivekananda Budamagunta, Thomas C. Foster, Daohong Zhou
Summary: Immunosenescence is a complex phenomenon that leads to age-related immune dysfunction, resulting in various pathologies such as decreased ability to clear senescent and cancerous cells, increased autoimmune response, reduced ability to combat pathogens, and diminished response to vaccination. Cellular senescence and immunosenescence have not been extensively explored in terms of their relationship, yet in the context of the ongoing pandemic, immunosenescence has become a topic of great importance.
Article
Biochemistry & Molecular Biology
Linshan Xu, Yuyang Wang, Jianping Wang, Jianglong Zhai, Li Ren, Guoying Zhu
Summary: Cellular senescence and its associated secretory phenotype (SASP) are considered potential therapeutic targets for aging-related diseases. Inducing cellular senescence in osteocyte-like cells led to increased expression of senescence and SASP markers, disrupting the differentiation potential of bone marrow mesenchymal stem cells (BMSCs) through paracrine signaling.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Giorgia Manni, Sandra Buratta, Maria Teresa Pallotta, Davide Chiasserini, Alessandro Di Michele, Carla Emiliani, Stefano Giovagnoli, Luisa Pascucci, Rita Romani, Ilaria Bellezza, Lorena Urbanelli, Francesca Fallarino
Summary: Extracellular vesicles (EVs) are membrane-enclosed particles secreted by cells and circulating in body fluids. They not only serve as a tool for waste disposal, but also transmit cell signals. Aging is characterized by genomic instability, telomere erosion, epigenetic alteration, and defective proteostasis. Studies have shown that EVs released in cell models are associated with these aging hallmarks and can spread senescence signals. Furthermore, EVs have great potential as anti-aging tools. Although this is a recent field, altered EV secretion in aging suggests it as an emerging hallmark of aging.
Review
Cell Biology
Yun-Shan Wu, Shan Liang, Dong-Yi Li, Jun-Hao Wen, Ji-Xin Tang, Hua-Feng Liu
Summary: Precise regulation of cell cycle is crucial for tissue homeostasis and development, while dysregulation of renal cell cycle is closely associated with renal fibrosis. Understanding and treating cell cycle dysregulation in renal cells is essential for the potential therapeutic strategies to halt or reverse renal fibrosis progression.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Kaori Suzuki, Etsuo A. Susaki, Isao Nagaoka
Summary: This review discusses the roles of cellular senescence and bacterial infection in the pathogenesis of atherosclerosis. The study finds that bacterial infections and cellular senescence occur at the sites of atherosclerotic lesions, and lipopolysaccharide from Gram-negative bacteria can induce and enhance cellular senescence and the associated inflammatory properties, thus contributing to the development of atherosclerosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Xuan Zhang, Ying Dong, Wan-chen Li, Bi-xi Tang, Jia Li, Yi Zang
Summary: This study investigated the potential of roxithromycin in treating pulmonary fibrosis by targeting senescent cells. Results showed that roxithromycin could selectively kill senescent cells, inhibit senescent cell-induced lung fibroblast activation, attenuate lung injury, inflammation, and fibrosis, and achieve these effects by inhibiting the NOX4 pathway.
ACTA PHARMACOLOGICA SINICA
(2021)
Article
Biochemistry & Molecular Biology
Na Zhang, Mengjie Shang, Hongxin Li, Lan Wu, Meichen Dong, Baiqu Huang, Jun Lu, Yu Zhang
Summary: Chemotherapy induces tumor cell senescence but also triggers tumor recurrence. This study found that dual inhibition of H3K9 and H3K27 methylation can effectively induce senescence of highly metastatic tumor cells without triggering SASP, offering a new therapeutic approach.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biotechnology & Applied Microbiology
Yuhao Wu, Shiwei Shen, Yifeng Shi, Naifeng Tian, Yifei Zhou, Xiaolei Zhang
Summary: In this article, the involvement of senescence in intervertebral disc degeneration (IVDD) is reviewed, and the potential of senolytics in eliminating senescent disc cells and alleviating IVDD is discussed. A table listing potential drugs for IVDD therapy is also provided.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Cell & Tissue Engineering
Frits Hulshof, Carolien Schophuizen, Milos Mihajlovic, Clemens van Blitterswijk, Rosalinde Masereeuw, Jan de Boer, Dimitrios Stamatialis
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2018)
Article
Cell & Tissue Engineering
N. V. Chevtchik, M. Mihajlovic, M. Fedecostante, L. Bolhuis-Versteeg, J. Sastre Torano, R. Masereeuw, D. Stamatialis
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2018)
Article
Biochemistry & Molecular Biology
Marko Mihajlovic, Milos Mihajlovic, Patricia Y. W. Dankers, Rosalinde Masereeuw, Rint P. Sijbesma
MACROMOLECULAR BIOSCIENCE
(2019)
Review
Engineering, Biomedical
Cecile Legallais, Dooli Kim, Sylvia M. Mihaila, Milos Mihajlovic, Marina Figliuzzi, Barbara Bonandrini, Simona Salerno, Fjodor A. Yousef Yengej, Maarten B. Rookmaaker, Natalia Sanchez Romero, Pilar Sainz-Arnal, Ulysse Pereira, Mattia Pasqua, Karin G. F. Gerritsen, Marianne C. Verhaar, Andrea Remuzzi, Pedro M. Baptista, Loredana De Bartolo, Rosalinde Masereeuw, Dimitrios Stamatialis
ADVANCED HEALTHCARE MATERIALS
(2018)
Review
Pharmacology & Pharmacy
Milos Mihajlovic, Kimberley E. Wever, Thom K. van der Made, Rob B. M. de Vries, Luuk B. Hilbrands, Rosalinde Masereeuw
PHARMACOLOGY & THERAPEUTICS
(2019)
Article
Chemistry, Medicinal
Emma van Groesen, Cornelis J. Slingerland, Paolo Innocenti, Milos Mihajlovic, Rosalinde Masereeuw, Nathaniel Martin
Summary: Vancomycin binds to lipid II to exert its antimicrobial effect, but cannot pass through the outer membrane of Gram-negative bacteria. Conjugation of vancomycin with OM disrupting PMEN results in hybrid vancomyxins with improved activity against Gram-negative strains and maintained antimicrobial effect against Gram-positive strains. The hybrid antibiotics show reduced nephrotoxicity compared to clinically used polymyxin antibiotics.
ACS INFECTIOUS DISEASES
(2021)
Article
Biochemistry & Molecular Biology
Marko Mihajlovic, Margot Rikkers, Milos Mihajlovic, Martina Viola, Gerke Schuiringa, Blessing C. Ilochonwu, Rosalinde Masereeuw, Lucienne Vonk, Jos Malda, Keita Ito, Tina Vermonden
Summary: This study investigated the fabrication of chondroitin sulfate/hyaluronic acid-based DN hydrogels and demonstrated the tunability of their viscoelasticity and dynamic character through composition changes. The DN hydrogels, with their reversible, dynamic covalent bonds, are suitable for bioprinting and injectability, while also mimicking the mechanical properties of cartilage tissue.
Article
Biochemistry & Molecular Biology
Milos Mihajlovic, Merle M. Krebber, Yi Yang, Sabbir Ahmed, Valeria Lozovanu, Daria Andreeva, Marianne C. Verhaar, Rosalinde Masereeuw
Summary: This study demonstrated that PBUTs induce inflammasome-mediated IL-1 beta production in proximal tubule cells via oxidative stress and NF-kappa B signaling, suggesting their involvement in disease-associated inflammatory processes.
Article
Biochemistry & Molecular Biology
Milos Mihajlovic, Mathieu Vinken
Summary: Drug-induced liver injury is often associated with mitochondrial perturbation and dysfunction. While many methods have been developed to study mitochondrial function, there is a need for more liver physiology-based models and approaches to predict potential toxicity and reduce clinical complications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Toxicology
Bruna dos Santos Rodrigues, Kaat Leroy, Milos Mihajlovic, Sybren De Boever, Sarah Vanbellingen, Bruno Cogliati, Joeri L. Aerts, Mathieu Vinken
Summary: Currently, there are no validated in vitro assays for testing the non-genotoxic carcinogenic potential of chemicals. Therefore, the two-year rodent bioassay remains the standard method for identifying these chemicals. Transcriptomic and proteomic analyses have provided a comprehensive understanding of non-genotoxic carcinogenesis, but functional changes at the transcriptional and translational levels have not been addressed. This study tested proposed in vitro biomarkers of non-genotoxic hepatocarcinogenicity using a 3-dimensional model, and found that spheroid cultures of human liver cells may be suitable tools for investigating chemical-induced hepatocarcinogenicity, but the mechanisms and read-outs studied are not eligible biomarkers for detecting non-genotoxic carcinogens.
ARCHIVES OF TOXICOLOGY
(2023)
Article
Food Science & Technology
Yi Yang, Milos Mihajlovic, Manoe J. Janssen, Rosalinde Masereeuw
Summary: This study investigated the role of uremic toxin indoxyl sulfate (IS) in accelerating cellular senescence and promoting kidney fibrosis. The findings suggest that IS accelerates senescence in proximal tubule epithelial cells through TNF-alpha and NF-kappa B signaling early on, and the epithelial-mesenchymal transition process at later time points.
Review
Biochemistry & Molecular Biology
Yi Yang, Milos Mihajlovic, Rosalinde Masereeuw
Summary: Senescence of kidney tubule cells plays a significant role in the progression of CKD, and the accumulation of PBUTs may promote senescence in these cells, leading to kidney fibrosis.
Meeting Abstract
Cell & Tissue Engineering
Milos Mihajlovic, Merle Krebber, Koen Westphal, Silvia Mihaila, Rolf Sparidans, Danilo Donato, Karin Gerritsen, Markus Storr, Rosalinde Masereeuw
TISSUE ENGINEERING PART A
(2022)
Meeting Abstract
Cell & Tissue Engineering
Yi Yang, Milos Mihajlovic, Floris Valentijn, Tri Q. Nguyen, Roel Goldschmeding, Rosalinde Masereeuw
TISSUE ENGINEERING PART A
(2022)