Article
Oncology
Fangyu Chen, Bing Xu, Jie Li, Xi Yang, Junjie Gu, Xijuan Yao, Xinchen Sun
Summary: The study revealed that hypoxic tumor cell-derived exosomal miR-340-5p contributes to radioresistance in OSCC by targeting KLF10/UVRAG. The findings suggest that miR-340-5p could be a potential biomarker and therapeutic target for enhancing radiosensitivity in OSCC. Moreover, metformin was found to increase KLF10 expression and improve radiosensitivity in OSCC, offering a promising therapeutic option for treatment.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2021)
Article
Biotechnology & Applied Microbiology
Laiya Lu, Jingyi Wang, Aoyuan Fan, Pei Wang, Runzhi Chen, Laibing Lu, Feng Yin
Summary: The study demonstrates that SMSC-EVs deliver miR-26a-5p into chondrocytes to up-regulate miR-26a-5p and inhibit PTEN, thereby inhibiting apoptosis and inflammation, ameliorating cartilage damage of OA.
JOURNAL OF GENE MEDICINE
(2021)
Article
Environmental Sciences
Yifei Qin, Jing Zhang, Ricardo David Avellan-Llaguno, Xu Zhang, Qiansheng Huang
Summary: DEHP exposure promotes the secretion of miR-26a-5p in sEV, subsequently enhancing the EMT, migration, and invasion ability in neighboring normal cells.
ENVIRONMENTAL POLLUTION
(2021)
Article
Cell Biology
Dan Zhang, Jian Du, Min Yu, Linna Suo
Summary: The specific effects and underlying mechanism of urine-derived stem cells-extracellular vesicles (USCs-EVs) on osteoporosis in diabetes rats were studied. It was found that USCs-EVs carrying miR-26a-5p activated the HIF-1α/VEGFA pathway through HDAC4 inhibition to prevent diabetic osteoporosis (DOP).
CELL BIOLOGY AND TOXICOLOGY
(2023)
Article
Pharmacology & Pharmacy
Jing Zhang, Juan Gao, Xianlong Li, Dengna Lin, Zhihui Li, Jialei Wang, Junfeng Chen, Zhiliang Gao, Bingliang Lin
Summary: The efficacy of human bone marrow MSC-derived sEVs in treating ALF was investigated, and the molecular mechanisms involved in regulating hepatocyte proliferation and apoptosis were explored. The results showed that BMSC-sEVs improved survival rates and reduced liver injury in ALF mice. This effect was achieved by upregulating miR-20a-5p, which targeted the PTEN/AKT signaling pathway to reduce hepatocyte apoptosis and promote cell proliferation.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Immunology
Jiaxin Duan, Hao Li, Changyuan Wang, Jialin Yao, Yue Jin, Jianyu Zhao, Yukun Zhang, Mozhen Liu, Huijun Sun
Summary: This study investigated the role of EVs-encapsulated miR-16-5p from BMSCs in osteogenic differentiation. The results showed that EVs-encapsulated miR-16-5p from BMSCs could promote osteogenic differentiation. Additionally, miR-16-5p mediated its effects by regulating Axin2, a scaffolding protein of GSK3 beta. This study provides evidence that EVs-encapsulated miR-16-5p from BMSCs could promote osteogenic differentiation by repressing Axin2.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Cell Biology
Meiling Wu, Xuemei Liu, Zihan Li, Xiaoyao Huang, Hao Guo, Xiaohe Guo, Xiaoxue Yang, Bei Li, Kun Xuan, Yan Jin
Summary: The study demonstrated that SHED aggregate-derived exosomes significantly improved pulp tissue regeneration and angiogenesis by regulating miR-26a and the TGF-beta/SMAD2/3 signaling pathway to enhance vascular formation in SHED and HUVECs.
CELL PROLIFERATION
(2021)
Article
Oncology
Dennis Gerloff, Stefanie Kewitz-Hempel, Gerd Hause, Jovine Ehrenreich, Linda Golle, Tim Kingreen, Cord Sunderkoetter
Summary: Extracellular vesicles (EVs) play an important role in intercellular communication, influencing the function and phenotype of different cell types within the tumor microenvironment and promoting tumor progression. This study identified miRNAs enriched in both melanoma cells and melanoma cell-derived EVs, and showed that these miRNAs were also elevated in serum-derived EVs of patients with metastatic melanoma, suggesting their potential as prognostic or diagnostic liquid biopsy markers in melanoma.
FRONTIERS IN ONCOLOGY
(2022)
Article
Medicine, Research & Experimental
Jinli Ding, Yan Zhang, Xiaopeng Cai, Yi Zhang, Sisi Yan, Jiayu Wang, Sainan Zhang, Tailang Yin, Chaogang Yang, Jing Yang
Summary: The study revealed that M1 macrophages suppressed trophoblast EMT and reduced their migration and invasion abilities by secreting extracellular vesicles (EVs). miR-146a-5p and miR-146b-5p were identified as the most upregulated miRNAs in trophoblasts treated with M1-EVs, playing a role in inhibiting trophoblast EMT by directly suppressing TNF receptor-associated factor 6 (TRAF6) expression. Aberrant expression of miR-146a-5p, miR-146b-5p, and TRAF6 was found in placental villous tissues from patients with RSA, with negative correlations between miR-146a-5p/miR-146b-5p and TRAF6 expression levels.
Article
Medicine, Research & Experimental
Xiaoying Wang, Lili Jiang, Qifang Liu
Summary: This study demonstrated that human mesenchymal stem cell-derived extracellular vesicles (hMSC-EVs) inhibit ovarian cancer cell growth, migration, invasion, and chemotherapy resistance by carrying miR-18a-5p. miR-18a-5p targets NACC1, and the activation of the AKT/mTOR pathway is involved in this process.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Engineering, Biomedical
Yanhua Lan, Huizhi Xie, Qianrui Jin, Xiaomin Zhao, Yang Shi, Yanyan Zhou, Zihe Hu, Yi Ye, Xiaoyuan Huang, Yingjia Sun, Zhuo Chen, Zhijian Xie
Summary: This study reveals that extracellular vesicles (EVs) derived from Nell1-modified bone marrow stem cells (BMSCs) have a stronger ability to promote BMSC osteogenesis due to the downregulation of miR-25-5p. Additionally, the 3D-Nell1/EV hydrogel system is shown to be beneficial for bone regeneration in vivo, possibly because of the slow and continuous release of EVs at a high concentration in the bone defect area.
BIOACTIVE MATERIALS
(2022)
Article
Cell Biology
Yiqing Xu, Yulong Ge, Xuming Chen, Yingzi Zhang, Huanliang Chen, Dongli Liu, Yue Lu, Yong Liu, Wenzhi Tu
Summary: This study investigated the bystander effect of hypoxia on radiation-induced rectal injury. The results showed that secreted factors from hypoxic HIEC cells promoted apoptosis in normoxic HIEC cells. Further analysis revealed that miR-122-5p played a critical role in radiation-induced rectal injury. This study provides potential therapeutic targets for the treatment of radiation-induced rectal injury.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Yizhong Peng, Xuanzuo Chen, Sheng Liu, Wei Wu, Hongyang Shu, Shuo Tian, Yan Xiao, Kanglu Li, BaiChuan Wang, Hui Lin, Xiangcheng Qing, Zengwu Shao
Summary: The senescence of nucleus pulposus stem cells (NPSCs) plays a critical role in the progression of intervertebral disc degeneration (IDD). Stem cell-derived extracellular vesicles (EV) alleviate cellular senescence, but the underlying mechanism is unclear. RGD, a tripeptide that strongly binds integrins expressed on EV membranes, is used to develop an RGD-complexed nucleus pulposus matrix hydrogel (RGD-DNP) that enhances the therapeutic effects of small EV (sEV).
Article
Cell Biology
Kyoung Soo Lee, Jeongmi Lee, Hark Kyun Kim, Seung Ho Yeom, Chang Hee Woo, Youn Jae Jung, Ye Eun Yun, So Young Park, Jihoon Han, Eunae Kim, Jae Hoon Sul, Jae Min Jung, Jae Hyung Park, Ji Suk Choi, Yong Woo Cho, Dong-Gyu Jo
Summary: EVs derived from adipose tissue-derived stem cells (ASGEVs) show promising therapeutic effects on osteoporosis by containing rich growth factors and cytokines related to bone metabolism and MSC migration. They attenuate bone loss, inhibit osteoclast differentiation, and promote MSC migration. High expression of OPG in ASCEVs is critical for the therapeutic effects.
JOURNAL OF EXTRACELLULAR VESICLES
(2021)
Article
Biotechnology & Applied Microbiology
Chongzhi Pan, Wenzhou Huang, Qi Chen, Jiu Xu, Guoyu Yao, Bin Li, Tianlong Wu, Changchang Yin, Xigao Cheng
Summary: The study demonstrates that EVs derived from hMSCs overexpressing lncRNA malat-1 can reduce inflammation and apoptosis in chondrocytes, promoting their proliferation and migration while preventing cartilage degeneration in an osteoarthritis model. These findings suggest that hMSCs(malat-1)-EVs could be a potential therapeutic option for patients with OA.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)